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Caffeic Acid and Ferulic Acid Inhibit UVA‐Induced Matrix Metalloproteinase‐1 through Regulation of Antioxidant Defense System in Keratinocyte HaCaT Cells
Abstract
Ultraviolet A (UVA) plays a vital role in the pathogenesis of premature skin aging through keratinocyte cytotoxicity and degradation of collagen, a main component of the extracellular matrix providing structural support. Oxidative stress caused by UVA irradiation can mediate induction of matrix metalloprotease‐1 (MMP‐1), a major enzyme responsible for collagen damage. Protection against UV‐mediated disturbance of antioxidant defense system has been proposed as a possible mechanism by which botanical compounds slow down skin aging process. This study therefore aimed to assess inhibitory effects of caffeic acid (CA) and ferulic acid (FA), powerful plant‐based phenolic antioxidants, on UVA‐induced cytotoxicity and MMP‐1 activity and mRNA level through modulation of antioxidant defense mechanism in immortalized human keratinocyte (HaCaT) cells. Pretreatment of the cells with CA or FA prior to UVA irradiation inhibited cytotoxicity, induction of MMP‐1 activity and mRNA and oxidant formation. Moreover, CA and FA were able to up‐regulate glutathione (GSH) content, γ‐glutamate cysteine ligase (γ‐GCL) mRNA as well as activities and mRNA expression of catalase and glutathione peroxidase (GPx) in irradiated cells. In conclusion, CA and FA provided protective effects on UVA‐mediated MMP‐1 induction in HaCaT cells possibly through restoration of antioxidant defense system at the cellular and molecular level.
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“In addition to its antioxidant properties, ferulic acid inhibits matrix metalloproteinases and promotes the production of tissue inhibitors of metalloproteinases, which likely contributed to the increased skin elasticity observed in this study.26,27”
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“In addition to its antioxidant properties, ferulic acid inhibits matrix metalloproteinases and promotes the production of tissue inhibitors of metalloproteinases, which likely contributed to the increased skin elasticity observed in this study.26,27”
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Metadata
- Authors
- Pluemsamran, T., Onkoksoong, T., & Panich, U.
- Affiliations
- Siriraj Hospital (TH)
- Corresponding author
- Panich, Uraiwan
- ORCID
- Panich, Uraiwan
- Venue
- Photochemistry and Photobiology
- Publisher
- Wiley
- Published
- 2012-04-20
- Volume
- 88
- Issue
- 4
- Pages
- 961-968
- ISSN
- 0031-8655 (ISSN-L)
- 1751-1097
- OpenAlex
- W2045951593
- Cited by
- 140
- References
- 31
- Citations by year
- 2026: 6 · 2025: 14 · 2024: 10 · 2023: 8 · 2022: 8 · 2021: 22 · 2020: 13 · 2019: 13 · 2018: 8 · 2017: 9 · 2016: 7 · 2015: 6 · 2014: 9 · 2013: 6 · 2012: 1
- FWCI
- 8.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Photochemistry and Photobiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Medicine › Biochemistry (medical) › Phytochemicals and Antioxidant Activities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Keywords
- caffeic acid, ferulic acid, UVA, MMP-1, keratinocytes, HaCaT keratinocytes, skin aging, oxidative stress, antioxidant defense, glutathione, gamma-glutamylcysteine synthetase, catalase, glutathione peroxidase, collagen degradation, phenolic antioxidants
- MeSH terms
- Antioxidants, Caffeic Acids, Catalase, Cell Line, Transformed, Coumaric Acids, Glutamate-Cysteine Ligase, Gene Expression Regulation, Glutathione, Glutathione Peroxidase, Humans, RNA, Messenger, Ultraviolet Rays, Skin Aging, Keratinocytes, Oxidative Stress, Matrix Metalloproteinase 1, Matrix Metalloproteinase Inhibitors
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