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ARGOS 8 variants generated by CRISPR ‐Cas9 improve maize grain yield under field drought stress conditions
Open accessAbstract
Maize ARGOS8 is a negative regulator of ethylene responses. A previous study has shown that transgenic plants constitutively overexpressing ARGOS8 have reduced ethylene sensitivity and improved grain yield under drought stress conditions. To explore the targeted use of ARGOS8 native expression variation in drought-tolerant breeding, a diverse set of over 400 maize inbreds was examined for ARGOS8 mRNA expression, but the expression levels in all lines were less than that created in the original ARGOS8 transgenic events. We then employed a CRISPR-Cas-enabled advanced breeding technology to generate novel variants of ARGOS8. The native maize GOS2 promoter, which confers a moderate level of constitutive expression, was inserted into the 5'-untranslated region of the native ARGOS8 gene or was used to replace the native promoter of ARGOS8. Precise genomic DNA modification at the ARGOS8 locus was verified by PCR and sequencing. The ARGOS8 variants had elevated levels of ARGOS8 transcripts relative to the native allele and these transcripts were detectable in all the tissues tested, which was the expected results using the GOS2 promoter. A field study showed that compared to the WT, the ARGOS8 variants increased grain yield by five bushels per acre under flowering stress conditions and had no yield loss under well-watered conditions. These results demonstrate the utility of the CRISPR-Cas9 system in generating novel allelic variation for breeding drought-tolerant crops.
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“improving the yield of maize crops under drought stress [181]”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [181]
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“The technique has already been successfully applied to crop plants to alter agriculturally important traits such as disease resistance (Wang et al., 2014) and drought tolerance (Shi et al., 2017).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Shi et al., 2017
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210
The B73 Maize Genome: Complexity, Diversity, and Dynamics
Schnable, P. S., Ware, D., Fulton, R. S., Stein, J. C., Wei, F., Pasternak, S., Liang, C., Zhang, J., Fulton, L., Graves, T., et al. (2009) · Science · doi:10.1126/science.1178534 · Cited by 4,096
Wang, Y., Cheng, X., Shan, Q., Zhang, Y., Liu, J., Gao, C., & Qiu, J.-L. (2014) · Nature Biotechnology · doi:10.1038/nbt.2969 · Cited by 2,072
Li, J., Norville, J. E., Aach, J., McCormack, M., Zhang, D., Bush, J., Church, G. M., & Sheen, J. (2013) · Nature Biotechnology · doi:10.1038/nbt.2654 · Cited by 1,533
Plant Breeding for Stress Environments
Blum, A. (2018) · doi:10.1201/9781351075718 · Cited by 1,309
Jiang, W., Zhou, H., Bi, H., Fromm, M., Yang, B., & Weeks, D. P. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt780 · Cited by 1,282
High-efficiency TALEN-based gene editing produces disease-resistant rice
Li, T., Liu, B., Spalding, M. H., Weeks, D. P., & Yang, B. (2012) · Nature Biotechnology · doi:10.1038/nbt.2199 · Cited by 1,149
Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana
Bleecker, A. B., Estelle, M. A., Somerville, C. R., & Kende, H. (1988) · Science · doi:10.1126/science.241.4869.1086 · Cited by 1,014
Precise genome modification in the crop species Zea mays using zinc-finger nucleases
Shukla, V. K., Doyon, Y., Miller, J. C., DeKelver, R. C., Moehle, E. A., Worden, S. E., Mitchell, J. C., Arnold, N. L., Gopalan, S. M., Meng, X., et al. (2009) · Nature · doi:10.1038/nature07992 · Cited by 983
Zhang, H., Zhang, J., Wei, P., Zhang, B., Gou, F., Feng, Z., Mao, Y., Yang, L., Zhang, H., Xu, N., et al. (2014) · Plant Biotechnology Journal · doi:10.1111/pbi.12200 · Cited by 799
Svitashev, S. K., Young, J. K., Schwartz, C., Gao, H., Falco, S. C., & Cigan, A. M. (2015) · PLANT PHYSIOLOGY · doi:10.1104/pp.15.00793 · Cited by 758
Self-citationTargeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System
Liang, Z., Zhang, K., Chen, K., & Gao, C. (2013) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2013.12.001 · Cited by 671
High-frequency, precise modification of the tomato genome
Čermák, T., Baltes, N. J., Čegan, R., Zhang, Y., & Voytas, D. F. (2015) · Genome biology · doi:10.1186/s13059-015-0796-9 · Cited by 667
Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice
Zhou, H., Liu, B., Weeks, D. P., Spalding, M. H., & Yang, B. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku806 · Cited by 609
Targeted genome modifications in soybean with CRISPR/Cas9
Jacobs, T. B., LaFayette, P. R., Schmitz, R. J., & Parrott, W. A. (2015) · BMC Biotechnology · doi:10.1186/s12896-015-0131-2 · Cited by 594
Zhou, J., Peng, Z., Long, J., Sosso, D., Liu, B., Eom, J., Huang, S., Liu, S., Cruz, C. V., Frommer, W. B., et al. (2015) · The Plant Journal · doi:10.1111/tpj.12838 · Cited by 571
Sun, Y., Zhang, X., Wu, C., He, Y., Ma, Y., Hou, H., Guo, X., Du, W., Zhao, Y., & Xia, L. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.01.001 · Cited by 535
Cas9-Guide RNA Directed Genome Editing in Soybean
Li, Z., Liu, Z.-B., Xing, A., Moon, B. P., Koellhoffer, J. P., Huang, L., Ward, R. T., Clifton, E., Falco, S. C., & Cigan, A. M. (2015) · PLANT PHYSIOLOGY · doi:10.1104/pp.15.00783 · Cited by 528
Nuccio, M. L., Wu, J., Mowers, R., Zhou, H.-P., Meghji, M., Primavesi, L. F., Paul, M. J., Chen, X., Gao, Y., Haque, E., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3277 · Cited by 488
Castiglioni, P., Warner, D., Bensen, R. J., Anstrom, D. C., Harrison, J. M., Stoecker, M., Abad, M. S., Kumar, G., Salvador, S., D’Ordine, R. L., et al. (2008) · PLANT PHYSIOLOGY · doi:10.1104/pp.108.118828 · Cited by 485
The Arabidopsis Auxin-Inducible Gene ARGOS Controls Lateral Organ Size
Hu, Y., Xie, Q., & Chua, N. (2003) · The Plant Cell · doi:10.1105/tpc.013557 · Cited by 456
Breeding drought-tolerant maize hybrids for the US corn-belt: discovery to product
Cooper, M., Gho, C., Leafgren, R., Tang, T., & Messina, C. (2014) · Journal of Experimental Botany · doi:10.1093/jxb/eru064 · Cited by 417
Creation of fragrant rice by targeted knockout of the Os BADH 2 gene using TALEN technology
Shan, Q., Zhang, Y., Chen, K., Zhang, K., & Gao, C. (2015) · Plant Biotechnology Journal · doi:10.1111/pbi.12312 · Cited by 383
Rapid reorganization of resistance gene homologues in cereal genomes
Leister, D., Kurth, J., Laurie, D. A., Yano, M., Sasaki, T., Devos, K. M., Graner, A., & Schulze‐Lefert, P. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.1.370 · Cited by 347
Ameline-Torregrosa, C., Wang, B. B., O’Bleness, M., Deshpande, S., Zhu, H., Roe, B. A., Young, N. D., & Cannon, S. B. (2007) · PLANT PHYSIOLOGY · doi:10.1104/pp.107.104588 · Cited by 299
Hays, D. B., Hwa, J., Mason, R. E., Morgan, G. D., & Finlayson, S. A. (2007) · Plant Science · doi:10.1016/j.plantsci.2007.03.004 · Cited by 294
CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots
Cai, Y., Chen, L., Liu, X., Sun, S., Wu, C., Jiang, B., Han, T., & Hou, W. (2015) · PLoS ONE · doi:10.1371/journal.pone.0136064 · Cited by 270
Efficient targeted mutagenesis in soybean by TALENs and CRISPR/Cas9
Du, H., Zeng, X., Zhao, M., Cui, X., Wang, Q., Yang, H., Cheng, H., & Yu, D. (2015) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2015.11.005 · Cited by 270
Heritable targeted mutagenesis in maize using a designed endonuclease
Gao, H., Smith, J., Yang, M., Jones, S., Djukanovic, V., Nicholson, M. G., West, A., Bidney, D. L., Falco, S. C., Jantz, D., et al. (2009) · The Plant Journal · doi:10.1111/j.1365-313x.2009.04041.x · Cited by 241
Self-citationHabben, J. E., Bao, X., Bate, N. J., DeBruin, J. L., Dolan, D., Hasegawa, D., Helentjaris, T. G., Lafitte, R. H., Lovan, N., Mo, H., et al. (2014) · Plant Biotechnology Journal · doi:10.1111/pbi.12172 · Cited by 226
Self-citationRichly, E., Kurth, J., & Leister, D. (2002) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a003984 · Cited by 224
Shi, J., Habben, J. E., Archibald, R. L., Drummond, B. J., Chamberlin, M. A., Williams, R. W., Lafitte, H. R., & Weers, B. P. (2015) · PLANT PHYSIOLOGY · doi:10.1104/pp.15.00780 · Cited by 223
Self-citationGene Loss and Movement in the Maize GenomePreprint
Lai, J., Ma, J., Swigoňová, Z., Ramakrishna, W., Linton, E. W., Llaca, V., Tanyolaç, B., Park, Y., Jeong, O., Bennetzen, J. L., et al. (2004) · Genome Research · doi:10.1101/gr.2701104 · Cited by 198
Gaffney, J., Schussler, J., Löffler, C., Cai, W., Paszkiewicz, S., Messina, C., Groeteke, J., Keaschall, J., & Cooper, M. (2015) · Crop Science · doi:10.2135/cropsci2014.09.0654 · Cited by 193
Genome-Level Evolution of Resistance Genes in Arabidopsis thaliana
Baumgarten, A. M., Cannon, S. B., Spangler, R., & May, G. (2003) · Genetics · doi:10.1093/genetics/165.1.309 · Cited by 170
Spatial analysis of multi-environment early generation variety trials
Cullis, B., Gogel, B., Verbyla, A., & Thompson, R. (1998) · Biometrics · doi:10.2307/2533991 · Cited by 168
Classification of Maize Environments Using Crop Simulation and Geographic Information Systems
Löffler, C. M., Wei, J., Fast, T., Gogerty, J., Langton, S., Bergman, M. K., Merrill, B., & Cooper, M. (2005) · Crop Science · doi:10.2135/cropsci2004.0370 · Cited by 165
Many or most genes in Arabidopsis transposed after the origin of the order BrassicalesPreprint
Freeling, M., Lyons, E. H., Pedersen, B. S., Alam, M. S., Ming, R., & Lisch, D. R. (2008) · Genome Research · doi:10.1101/gr.081026.108 · Cited by 157
Patterns of Segmental Duplication in the Human Genome
Zhang, L. (2004) · Molecular Biology and Evolution · doi:10.1093/molbev/msh262 · Cited by 132
A Segmental Gene Duplication Generated Differentially Expressed myb-Homologous Genes in Maize
Zhang, P., Chopra, S., & Peterson, T. (2000) · The Plant Cell · doi:10.1105/tpc.12.12.2311 · Cited by 128
Maize ARGOS1 (ZAR1) transgenic alleles increase hybrid maize yield
Guo, M., Rupe, M. A., Wei, J., Winkler, C., Goncalves-Butruille, M., Weers, B. P., Cerwick, S. F., Dieter, J. A., Duncan, K. E., Howard, R. J., et al. (2013) · Journal of Experimental Botany · doi:10.1093/jxb/ert370 · Cited by 122
Gordon-Kamm, W. J., Dilkes, B. P., Lowe, K. S., Hoerster, G., Sun, X., Ross, M., Church, L., Bunde, C., Farrell, J., Hill, P., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.142409899 · Cited by 109
Feng, G., Qin, Z., Yan, J., Zhang, X., & Hu, Y. (2011) · New Phytologist · doi:10.1111/j.1469-8137.2011.03710.x · Cited by 100
Artificial chromosome formation in maize (Zea mays L.)
Ananiev, E. V., Wu, C. C., Chamberlin, M. A., Svitashev, S. K., Schwartz, C. S., Gordon-Kamm, W. J., & Tingey, S. V. (2008) · Chromosoma · doi:10.1007/s00412-008-0191-3 · Cited by 85
Adjacent Sequences Influence DNA Repair Accompanying Transposon Excision in Maize
Scott, L., LaFoe, D., & Weil, C. F. (1996) · Genetics · doi:10.1093/genetics/142.1.237 · Cited by 84
The ARGOS gene family functions in a negative feedback loop to desensitize plants to ethylene
Rai, M. I., Wang, X., Thibault, D. M., Kim, H. J., Bombyk, M. M., Binder, B. M., Shakeel, S. N., & Schaller, G. E. (2015) · BMC Plant Biology · doi:10.1186/s12870-015-0554-x · Cited by 67
Physiological Effects of 1-Methylcyclopropene on Well-Watered and Water-Stressed Cotton Plants
Kawakami, E. M., Oosterhuis, D. M., & Snider, J. L. (2010) · Journal of Plant Growth Regulation · doi:10.1007/s00344-009-9134-3 · Cited by 66
Constitutive expression of the ARGOS gene driven by dahlia mosaic virus promoter in tobacco plants
Кулуев, Б. Р., Knyazev, A. V., Iljassowa, A. A., & Chemeris, A. V. (2011) · Russian Journal of Plant Physiology · doi:10.1134/s1021443711030083 · Cited by 63
de Pater, B. S., van der Mark, F., Rueb, S., Katagiri, F., Chua, N., Schilperoort, R. A., & Hensgens, L. A. M. (1992) · The Plant Journal · doi:10.1046/j.1365-313x.1992.00999.x · Cited by 55
Shi, J., Drummond, B. J., Wang, H., Archibald, R. L., & Habben, J. E. (2016) · PLANT PHYSIOLOGY · doi:10.1104/pp.16.00347 · Cited by 47
Self-citationRamos, O. M., & Ferrier, D. E. K. (2012) · International Journal of Evolutionary Biology · doi:10.1155/2012/846421 · Cited by 42
Wang, D., Yu, C., Zuo, T., Zhang, P., Weber, D. F., & Peterson, T. (2015) · Genetics · doi:10.1534/genetics.115.178210 · Cited by 21
Kawakami, E. M., Oosterhuis, D. M., Snider, J. L., & FitzSimons, T. R. (2013) · American Journal of Plant Sciences · doi:10.4236/ajps.2013.47171 · Cited by 16
Leibman, M., Shryock, J. J., Clements, M. J., Hall, M. A., Loida, P. J., McClerren, A. L., McKiness, Z. P., Phillips, J., Rice, E. A., & Stark, S. B. (2014) · Plant Biotechnology Journal · doi:10.1111/pbi.12202 · Cited by 15
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The Pivotal Role of Ethylene in Plant Growth
Dubois, M., den Broeck, L. V., & Inzé, D. (2018) · Trends in Plant Science · doi:10.1016/j.tplants.2018.01.003 · Cited by 842
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Jaganathan, D., Ramasamy, K., Sellamuthu, G., Jayabalan, S., & Venkataraman, G. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00985 · Cited by 619
Metadata
- Authors
- Shi, J., Gao, H., Wang, H., Lafitte, H. R., Archibald, R. L., Yang, M., Hakimi, S. M., Mo, H., & Habben, J. E.
- Affiliations
- Pioneer Hi-Bred (US)
- Corresponding author
- Shi, Jinrui
- ORCID
- Venue
- Plant Biotechnology Journal
- Publisher
- Wiley
- Published
- 2016-08-17
- Volume
- 15
- Issue
- 2
- Pages
- 207-216
- ISSN
- 1467-7644 (ISSN-L)
- 1467-7652
- PMC
- PMC5258859
- OpenAlex
- W2488393959
- Cited by
- 1,029
- References
- 56
- Citations by year
- 2026: 97 · 2025: 119 · 2024: 99 · 2023: 127 · 2022: 157 · 2021: 145 · 2020: 109 · 2019: 85 · 2018: 47 · 2017: 40 · 2016: 3
- FWCI
- 30.33
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Plant Biotechnology Journal (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Keywords
- maize, drought stress, grain yield, CRISPR/Cas9, ethylene response, promoter replacement, allelic variation, drought tolerance breeding, gene expression
- MeSH terms
- Gene Editing, Base Sequence, Edible Grain, Zea mays, Ethylenes, Plant Proteins, Promoter Regions, Genetic, RNA, Messenger, Stress, Physiological, Polymerase Chain Reaction, Gene Expression Regulation, Plant, Crops, Agricultural, Plants, Genetically Modified, Biodiversity, Droughts, CRISPR-Cas Systems, CRISPR-Associated Proteins
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