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Cited by 14Dermatology and Skin DiseasesAcne and Rosacea Treatments and EffectsOral microbiology and periodontitis research

Changes in the skin microbiome during male maturation from 0 to 25 years of age

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Hu, Xinyue, Tang, Meng, Dong, Kun, Zhou, Jin, Wang, Dexian, Song, Liya·2023·Skin Research and Technology
DOI 10.1111/srt.13432PMC PMC10493343
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Abstract

Background: Skin microorganisms co‐develop with the human body and age influences the skin microenvironment and thus the skin bacterial community. Objectives: To investigate the changes in the skin microbiota during male development. Methods: High‐throughput 16S ribosomal RNA pyrosequencing was utilized to analyze the differences in bacterial composition of the skin in healthy males aged 0–25 years. Results: There were significant differences in facial skin bacterial diversity (Shannon index) and richness (Chao index) among the 4 groups of subjects ( p < 0.05). Streptococcus, Staphylococcus, Cutibacterium are dominant in males during growth, and regular changes occur with age after birth. Further analysis of skin bacteria between the 4 groups showed that the bacterial abundance of Cutibacterium acnes and Staphylococcus epidermidis tended to increase with age, and the bacterial abundance of Streptococcus, Rothia mucilaginosa, and Staphylococcus hominis tended to decrease with age. Conclusions: There are some changes in cheek skin bacterial diversity during male development, and there is a relationship between skin bacterial changes and skin development processes.

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  • During organism growth, bacterial populations gradually adapt to the skin environment, and microbiota colonization continues until equilibrium is reached in adulthood.

    “During the organism’s growth, the bacterial populations gradually adapt to the skin environment, and the microbiota colonization continues until equilibrium is reached in adulthood [8,9,10].”

    From Recreating Human Skin In Vitro: Should the Microbiota Be Taken into Account? (2024) · cites this paper as [10]

  • Skin PAMPAs lack stratified epidermal tissue, skin macromolecules, cell populations, and appendages, and their stratum corneum-mimicking lipid mixture does not contain all lipid subclasses normally present in human skin.

    “In particular, they lack the stratified epidermal tissue and all the wide set of skin macromolecules, cell populations, and appendages, while the lipid mixture mimicking the stratum corneum does not contain all the lipid subclasses normally present in the human skin [9,10].”

    From Recreating Human Skin In Vitro: Should the Microbiota Be Taken into Account? (2024) · cites this paper as [10]

    The Discussion describes changes in the facial skin microbiome during male maturation, not the composition or limitations of skin PAMPAs. Searches for stratified epidermal tissue and for missing stratum corneum lipid subclasses found no passage addressing the claim. Page numbers were not available in our copy. (LLM, conf=0.77)

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Research works printed but not linked: 27

  1. [1]Gallo RL. Human skin is the largest epithelial surface for interaction with microbes. J Invest Dermatol. 2017; 137: 1213– 1214. 10.1016/j.jid.2016.11.045 28395897 PMC5814118 · DOI
  2. [2]Gao Z, Tseng CH, Pei ZH, Blaser MJ. Molecular analysis of human forearm superficial skin bacterial biota. Proc Nat Acad Sci USA. 2007; 104: 2927– 2932. 10.1073/pnas.0607077104 17293459 PMC1815283 · DOI
  3. [3]Grice EA, Kong HH, Renaud G, et al. A diversity profile of the human skin microbiota. Genome Res. 2008; 18: 1043– 1050. 10.1101/gr.075549.107 18502944 PMC2493393 · DOI
  4. [4]Costello EK, Lauber CL, Hamady M, Fierer N, Gordon JI, Knight R. Bacterial community variation in human body habitats across space and time. Science. 2009; 326: 1694– 1697. 10.1126/science.1177486 19892944 PMC3602444 · DOI · Paper page
  5. [5]Grice EA, Kong HH, Conlan S, et al. Topographical and temporal diversity of the human skin microbiome. Science. 2009; 324: 1190– 1192. 10.1126/science.1171700 19478181 PMC2805064 · DOI · Paper page
  6. [6]Dreno B, Araviiskaia E, Berardesca E, et al. Microbiome in healthy skin, update for dermatologists. J Eur Acad Dermatol Venereol. 2016; 30: 2038– 2047. 10.1111/jdv.13965 27735094 PMC6084363 · DOI · Paper page
  7. [7]Dominguez‐Bello MG, Costello EK, Contreras M, et al. Delivery mode shapes the acquisition and structure of the initial microbiota across multiple body habitats in newborns. Proc Natl Acad Sci USA. 2010; 107: 11971– 11975. 10.1073/pnas.1002601107 20566857 PMC2900693 · DOI · Paper page
  8. [9]Ladizinski B, McLean R, Lee KC, Elpern DJ, Eron L. The human skin microbiome. Int J Dermatol. 2014; 53: 1177– 1179. 10.1111/ijd.12609 25070027 · DOI
  9. [10]Mueller NT, Bakacs E, Combellick J, Grigoryan Z, Dominguez‐Bello MG. The infant microbiome development: mom matters. Trends Mol Med. 2015; 21: 109– 117. 10.1016/j.molmed.2014.12.002 25578246 PMC4464665 · DOI · Paper page
  10. [11]Mathieu A, Delmont TO, Vogel TM, Robe P, Nalin R, Simonet P. Life on human surfaces: skin metagenomics. PLoS One. 2013; 8: e65288. doi: ARTN e6528. 10.1371/journal.pone.0065288 23776466 PMC3680502 · DOI
  11. [12]Leyden JJ, McGinley KJ, Mills OH, Kligman AM. Age‐related changes in the resident bacterial flora of the human face. J Invest Dermatol. 1975; 65: 379– 381. 10.1111/1523-1747.ep12607630 1176788 · DOI · Paper page
  12. [13]Somerville DA. The normal flora of the skin in different age groups. Br J Dermatol. 1969; 81: 248– 258. 10.1111/j.1365-2133.1969.tb13976.x 5778713 · DOI · Paper page
  13. [14]Zheng Y, Wang Q, Ma L, et al. Alterations in the skin microbiome are associated with disease severity and treatment in the perioral zone of the skin of infants with atopic dermatitis. Eur J Clin Microbiol Infect Dis. 2019; 38: 1677– 1685. 10.1007/s10096-019-03598-9 31152265 · DOI
  14. [15]Park J, Schwardt NH, Jo J, et al. Shifts in the skin bacterial and fungal communities of healthy children transitioning through puberty. J Invest Dermatol. 2022; 142(1): 212– 219. 10.1016/j.jid.2021.04.034 34252398 PMC8688298 · DOI
  15. [16]Giusti F, Martella A, Bertoni L, Seidenari S. Skin barrier, hydration, and pH of the skin of infants under 2 years of age. Pediatr Dermatol. 2001; 18: 93– 96. 10.1046/j.1525-1470.2001.018002093.x 11358544 · DOI
  16. [17]Powers CE, McShane DB, Gilligan PH, Burkhart CN, Morrell DS. Microbiome and pediatric atopic dermatitis. J Dermatol. 2015; 42: 1137– 1142. 10.1111/1346-8138.13072 26388516 · DOI
  17. [18]Kim G, Kim M, Kim M, et al. Spermidine‐induced recovery of human dermal structure and barrier function by skin microbiome. Commun Biol. 2021; 4: 231. 10.1038/s42003-020-01619-4 33608630 PMC7895926 · DOI
  18. [19]Leung MHY, Wilkins D, Lee PKH. Insights into the pan‐microbiome: skin microbial communities of Chinese individuals differ from other racial groups. Sci Rep. 2015; 5: 11845. 10.1038/srep11845 26177982 PMC4503953 · DOI
  19. [20]Wang R, Farhat M, Na J, Li R, Wu Y. Bacterial and fungal microbiome characterization in patients with rosacea and healthy controls. Br J Dermatol. 2020; 183: 1112– 1114. 10.1111/bjd.19315 32533846 · DOI
  20. [21]Zheng Y, Hunt RL, Villaruz AE, et al. Commensal Staphylococcus epidermidis contributes to skin barrier homeostasis by generating protective ceramides. Cell Host Microbe. 2022; 30: 301– 313.e9. 10.1016/j.chom.2022.01.004 35123653 PMC8917079 · DOI
  21. [22]Cogen AL, Yamasaki K, Muto J, et al. Staphylococcus epidermidis antimicrobial delta‐toxin (phenol‐soluble modulin‐gamma) cooperates with host antimicrobial peptides to kill group A Streptococcus. PLoS One. 2010; 5: e8557. 10.1371/journal.pone.0008557 20052280 PMC2796718 · DOI · Paper page
  22. [23]Naik S, Bouladoux N, Wilhelm C, et al. Compartmentalized control of skin immunity by resident commensals. Science. 2012; 337: 1115– 1119. 10.1126/science.1225152 22837383 PMC3513834 · DOI · Paper page
  23. [24]Kurokawa I, Danby FW, Ju Q, et al. New developments in our understanding of acne pathogenesis and treatment. Exp Dermatol. 2009; 18: 821– 832. 10.1111/j.1600-0625.2009.00890.x 19555434 · DOI · Paper page
  24. [25]Bhate K, Williams HC. Epidemiology of acne vulgaris. Br J Dermatol. 2013; 168: 474– 485. 10.1111/bjd.12149 23210645 · DOI
  25. [26]Iinuma K, Sato T, Akimoto N, et al. Involvement of Propionibacterium acnes in the augmentation of lipogenesis in hamster sebaceous glands in vivo and in vitro. J Invest Dermatol. 2009; 129: 2113– 2119. 10.1038/jid.2009.46 19282842 · DOI
  26. [27]Zouboulis CC, Jourdan E, Picardo M. Acne is an inflammatory disease and alterations of sebum composition initiate acne lesions. J Eur Acad Dermatol Venereol. 2014; 28: 527– 532. 10.1111/jdv.12298 24134468 · DOI · Paper page
  27. [28]Giacomoni PU, Mammone T, Teri M. Gender‐linked differences in human skin. J Dermatol Sci. 2009; 55: 144– 149. 10.1016/j.jdermsci.2009.06.001 19574028 · DOI

Cited by

Sorted by citations

Authors
Hu, X., Tang, M., Dong, K., Zhou, J., Wang, D., & Song, L.
Affiliations
Beijing Technology and Business University (CN)
Corresponding author
Zhou, Jin
ORCID
Venue
Skin Research and Technology
Publisher
Wiley
Published
2023-09
Volume
29
Issue
9
Pages
e13432-e13432
ISSN
  • 0909-752X (ISSN-L)
  • 1600-0846
DOI
10.1111/srt.13432
PMC
PMC10493343
OpenAlex
W4386615087
Cited by
14
References
1
Citations by year
2026: 6 · 2025: 3 · 2024: 3 · 2023: 2
FWCI
3.86
Type
journal article
Language
en
Open access
Licence
Locations
  • Skin Research and Technology (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
  • Health Sciences › Medicine › Dermatology › Acne and Rosacea Treatments and Effects
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
Keywords
skin microbiome, male maturation, age-related changes, bacterial diversity, Propionibacterium acnes, Staphylococcus epidermidis, Streptococcus, Rothia mucilaginosa, Staphylococcus hominis, skin development
MeSH terms
Adolescent, Adult, Child, Child, Preschool, Humans, Infant, Infant, Newborn, Male, Skin, Staphylococcus hominis, Young Adult, Microbiota

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