Alterations in insulin receptor signalling in the rat epitrochlearis muscle upon cessation of voluntary exercise
Abstract
The major purpose of this study was to elucidate mechanisms by which decreasing enhanced physical activity induces decreased insulin sensitivity in skeletal muscle. Rats with access to voluntary running wheels for 3 weeks had their wheels locked for 5 h (WL5), 29 h (WL29), or 53 h (WL53); a separate group of rats never had wheel access (sedentary, SED). Relative to WL5, submaximal insulin-stimulated 2-deoxyglucose uptake into the epitrochlearis muscle was lower in WL53 and SED. Insulin binding, insulin receptor beta-subunit (IRbeta) protein level, submaximal insulin-stimulated IRbeta tyrosine phosphorylation, and glucose transporter-4 protein level were each lower in both WL53 and SED than in WL5 and WL29. Akt/protein kinase B Ser(473) phosphorylation was lower in WL53 and SED than in WL5. Protein levels of protein tyrosine phosphatase-1B, Src homology phosphatase-2, and protein kinase C- did not vary among groups. The amount of protein tyrosine phosphatase-1B, Src homology phosphatase-2, and protein kinase C- associated with IRbeta in insulin-stimulated muscle also did not differ among the four groups. The mean of SED and WL53 had a significantly higher IRbeta-associated protein tyrosine phosphatase-1B than the mean of WL5 and WL29. The enclosure of multiple changes (decreases in insulin binding, IRbeta protein, IRbeta tyrosine phosphorylation, and glucose transporter-4 protein) in the epitrochlearis muscle within the 29th to 53rd hour after cessation of voluntary wheel running raises the possibility that a single regulatory event could be responsible for the coordinated decrease.
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In rats, epididymal fat mass at 53 hours of wheel lock was 25% greater than at 5 hours.
“Further, the epididymal adipose tissue mass at WL53 weighed 25% more than at WL5 in the same rats as studied for insulin sensitivity above.”
From Lack of Exercise Is a Major Cause of Chronic Diseases (2012) · cites this paper as [293]
In rats, 53 hours of wheel lock reduced submaximal insulin sensitivity and increased triglyceride storage in visceral fat.
“Within 53 hrs two major changes in functional capacities were observed, decreased submaximal insulin sensitivity and enhanced storage of TG in visceral adipose tissue.”
From Lack of Exercise Is a Major Cause of Chronic Diseases (2012) · cites this paper as [293]
Rats that cease 3 weeks of voluntary wheel running drop their submaximal insulin-stimulated glucose uptake to sedentary values on the second day of no running in skeletal muscle normally recruited during wheel running.
“Like humans, rats who cease 3 weeks of voluntary wheel running drop their submaximal insulin-stimulated glucose uptake to sedentary values on the 2nd day of no running in skeletal muscle normally recruited during wheel running (293).”
From Lack of Exercise Is a Major Cause of Chronic Diseases (2012) · cites this paper as [293]
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Cites
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Lack of Exercise Is a Major Cause of Chronic Diseases
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Self-citationRole of Inactivity in Chronic Diseases: Evolutionary Insight and Pathophysiological Mechanisms
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- Authors
- Kump, D. S., & Booth, F. W.
- Affiliations
- University of Missouri (US)
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 2005-01-20
- Volume
- 562
- Issue
- 3
- Pages
- 829-838
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- OpenAlex
- W1589632843
- Cited by
- 105
- References
- 4
- Citations by year
- 2024: 3 · 2023: 6 · 2022: 2 · 2021: 1 · 2020: 4 · 2019: 3 · 2018: 1 · 2017: 4 · 2016: 5 · 2015: 10 · 2014: 10 · 2013: 5 · 2012: 10
- FWCI
- 2.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Missouri
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR019393)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Physiology › Physical Activity and Health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Keywords
- insulin receptor signaling, insulin sensitivity, skeletal muscle, voluntary wheel running, exercise cessation, epitrochlearis muscle, insulin receptor beta-subunit, tyrosine phosphorylation, glucose transporter 4, Akt/protein kinase B, PTP1B, protein kinase C, 2-deoxyglucose uptake, sedentary behavior
- MeSH terms
- Adaptation, Physiological, Animals, Deoxyglucose, Exercise Test, Physical Exertion, Insulin, Physical Conditioning, Animal, Rats, Inbred F344, Receptor, Insulin, Rest, Signal Transduction, Muscle, Skeletal, Rats
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