Quantifying the confounding effect of pigmentation on measured skin tissue optical properties: a comparison of colorimetry with spatial frequency domain imaging
Open accessAbstract
Significance: Spatial frequency domain imaging (SFDI) is a wide-field diffuse optical imaging technique for separately quantifying tissue reduced scattering (μs ′ ) and absorption (μa) coefficients at multiple wavelengths, providing wide potential utility for clinical applications such as burn wound characterization and cancer detection. However, measured μs ′ and μa can be confounded by absorption from melanin in patients with highly pigmented skin. This issue arises because epidermal melanin is highly absorbing for visible wavelengths and standard homogeneous light–tissue interaction models do not properly account for this complexity. Tristimulus colorimetry (which quantifies pigmentation using the L * “lightness” parameter) can provide a point of comparison between μa, μs ′ , and skin pigmentation. Aim: We systematically compare SFDI and colorimetry parameters to quantify confounding effects of pigmentation on measured skin μs ′ and μa. We assess the correlation between SFDI and colorimetry parameters as a function of wavelength. Approach: μs ′ and μa from the palm and ventral forearm were measured for 15 healthy subjects with a wide range of skin pigmentation levels (Fitzpatrick types I to VI) using a Reflect RS® (Modulim, Inc., Irvine, California) SFDI instrument (eight wavelengths, 471 to 851 nm). L * was measured using a Chroma Meter CR-400 (Konica Minolta Sensing, Inc., Tokyo). Linear correlation coefficients were calculated between L * and μs ′ and between L * and μa at all wavelengths. Results: For the ventral forearm, strong linear correlations between measured L * and μs ′ values were observed at shorter wavelengths (R > 0.92 at ≤659 nm), where absorption from melanin confounded the measured μs ′ . These correlations were weaker for the palm (R < 0.59 at ≤659 nm), which has less melanin than the forearm. Similar relationships were observed between L * and μa. Conclusions: We quantified the effects of epidermal melanin on skin μs ′ and μa measured with SFDI. This information may help characterize and correct pigmentation-related inaccuracies in SFDI skin measurements.
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The penetration depth of light for imaging purposes and the results from medical wearable devices are affected by skin color.
“the penetration depth of light for imaging purposes and the results from medical wearable devices are affected by skin color.7–10”
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Cites
The validity and practicality of sun-reactive skin types I through VI
Fitzpatrick, T. B. (1988) · Archives of Dermatology · doi:10.1001/archderm.1988.01670060015008 · Cited by 4,339 · see citing works
MCML—Monte Carlo modeling of light transport in multi-layered tissues
Wang, L. V., Jacques, S. L., & Zheng, L. (1995) · Computer Methods and Programs in Biomedicine · doi:10.1016/0169-2607(95)01640-f · Cited by 3,330 · see citing works
Racial bias in pulse oximetry measurement
Sjoding, M. W., Dickson, R. P., Iwashyna, T. J., Gay, S. E., & Valley, T. S. (2020) · New England Journal of Medicine · doi:10.1056/nejmc2029240 · Cited by 1,114 · see citing works
Quantitation and mapping of tissue optical properties using modulated imaging
Cuccia, D. J., Bevilacqua, F. P., Durkin, A. J., Ayers, F. R., & Tromberg, B. J. (2009) · Journal of Biomedical Optics · doi:10.1117/1.3088140 · Cited by 659 · see citing works
Self-citationZonios, G., Bykowski, J., & Kollias, N. (2001) · Journal of Investigative Dermatology · doi:10.1046/j.0022-202x.2001.01577.x · Cited by 477 · see citing works
Ly, B. C. K., Dyer, E. B., Feig, J. L., Chien, A. L., & Del Bino, S. (2020) · Journal of Investigative Dermatology · doi:10.1016/j.jid.2019.11.003 · Cited by 453 · see citing works
Cuccia, D. J., Bevilacqua, F. P., Durkin, A. J., & Tromberg, B. J. (2005) · Optics Letters · doi:10.1364/ol.30.001354 · Cited by 430 · see citing works
Self-citationSkin Color Measurements in Terms of CIELAB Color Space Values
Weatherall, I. L., & Coombs, B. D. (1992) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12616156 · Cited by 366 · see citing works
Multilayer model of photon diffusion in skin
Schmitt, J. M., Wall, R. T., Zhou, G. X., & Walker, E. C. (1990) · Journal of the Optical Society of America A · doi:10.1364/josaa.7.002141 · Cited by 206 · see citing works
ANALYTICAL MODELING FOR THE OPTICAL PROPERTIES OF THE SKIN WITH IN VITRO AND IN VIVO APPLICATIONS
Wan, S., Anderson, R. R., & Parrish, J. A. (1981) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.1981.tb09391.x · Cited by 198 · see citing works
Zonios, G. I., & Dimou, A. D. (2006) · Optics Express · doi:10.1364/oe.14.008661 · Cited by 197 · see citing works
Determination of tissue optical properties by steady-state spatial frequency-domain reflectometry
D�gnitz, N., & Wagni�res, G. (1998) · Lasers in Medical Science · doi:10.1007/bf00592960 · Cited by 96 · see citing works
Saager, R. B., Balu, M., Crosignani, V., Sharif, A., Durkin, A. J., Kelly, K. M., & Tromberg, B. J. (2015) · Journal of Biomedical Optics · doi:10.1117/1.jbo.20.6.066005 · Cited by 76 · see citing works
Self-citationSaager, R. B., Truong, A., Durkin, A. J., & Cuccia, D. J. (2011) · Journal of Biomedical Optics · doi:10.1117/1.3597621 · Cited by 66 · see citing works
Self-citationRetrieval of optical properties of skin from measurement and modeling the diffuse reflectance
Douven, L. F. A., & Lucassen, G. W. (2000) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.388058 · Cited by 61 · see citing works
Fredriksson, I., Burdakov, O. P., Larsson, M., & Tomas Strömberg, A. (2013) · Journal of Biomedical Optics · doi:10.1117/1.jbo.18.12.127004 · Cited by 60 · see citing works
Practical applications of cutaneous colorimetry
Andreassi, L., & Flori, L. (1995) · Clinics in Dermatology · doi:10.1016/0738-081x(95)00069-r · Cited by 59 · see citing works
Jonasson, H., Fredriksson, I., Bergstrand, S., Östgren, C. J., Larsson, M., & Strömberg, T. (2018) · Journal of Biomedical Optics · doi:10.1117/1.jbo.23.12.121608 · Cited by 52 · see citing works
Skin Color and Pigmentation in Ethnic Skin
Visscher, M. O. (2016) · Facial Plastic Surgery Clinics of North America · doi:10.1016/j.fsc.2016.08.011 · Cited by 50 · see citing works
Spatial frequency domain spectroscopy of two layer media
Yudovsky, D., & Durkin, A. J. (2011) · Journal of Biomedical Optics · doi:10.1117/1.3640814 · Cited by 46 · see citing works
Self-citationVerdel, N., Marin, A., Milanič, M., & Majaron, B. (2019) · Biomedical Optics Express · doi:10.1364/boe.10.000944 · Cited by 45 · see citing works
Phan, T., Rowland, R., Ponticorvo, A., Le, B. C., Wilson, R. H., Sharif, S. A., Kennedy, G. T., Bernal, N. P., & Durkin, A. J. (2021) · Journal of Biomedical Optics · doi:10.1117/1.jbo.26.2.026001 · Cited by 38 · see citing works
Self-citationVasefi, F., MacKinnon, N. B., Saager, R. B., Kelly, K. M., Maly, T. J., Booth, N., Durkin, A. J., & Farkas, D. L. (2016) · Journal of Biomedical Optics · doi:10.1117/1.jbo.21.11.114001 · Cited by 31 · see citing works
Self-citationZonios, G. I., Dimou, A. D., Carrara, M., et al. (2009) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2009.00630.x · Cited by 31 · see citing works
Saager, R. B., Sharif, A., Kelly, K. M., & Durkin, A. J. (2016) · Journal of Biomedical Optics · doi:10.1117/1.jbo.21.5.057001 · Cited by 30 · see citing works
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Hidalgo, D. C., Olusanya, O., & Harlan, E. (2021) · The Lancet Respiratory Medicine · doi:10.1016/s2213-2600(21)00102-8 · Cited by 21 · see citing works
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Philip, K. E. J., Tidswell, R., & McFadyen, C. (2021) · BMJ · doi:10.1136/bmj.n298 · Cited by 16 · see citing works
Stier, A. C., Goth, W., Hurley, A., Brown, T., Feng, X., Zhang, Y., Lopes, F. C. P. S., Sebastian, K., Ren, P. Y., Fox, M. C., et al. (2021) · Journal of Biomedical Optics · doi:10.1117/1.jbo.26.9.096007 · Cited by 15 · see citing works
Horan, S. T., Gardner, A. R., Saager, R. B., Durkin, A. J., & Venugopalan, V. (2018) · Journal of Biomedical Optics · doi:10.1117/1.jbo.24.7.071607 · Cited by 14 · see citing works
Self-citationJonasson, H., Pettersson, A., Larsson, M., & Tomas Strömberg, A. (2011) · Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · doi:10.1117/12.873134 · Cited by 11 · see citing works
Jin, W., Rowland, R. A., Wilson, R. H., Phan, T. P., Le, B. C., Kennedy, G. T., & Durkin, A. J. (2021) · Photonics in Dermatology and Plastic Surgery · doi:10.1117/12.2579019 · Cited by 2 · see citing works
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Vasudevan, S., Vogt, W. C., Weininger, S., & Pfefer, T. J. (2024) · Communications Medicine · doi:10.1038/s43856-024-00550-7 · Cited by 46 · see citing works
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Shugar, A. L., Konger, R. L., Rohan, C. A., Travers, J. B., & Kim, Y. L. (2024) · Experimental Dermatology · doi:10.1111/exd.15076 · Cited by 1 · see citing works
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Hsieh, M.-C., LAI, R. H., Lin, Y., Hsu, C., Hsieh, P.-Y., Chen, W., Ching, C. T. S., & Liao, L. (2026) · iScience · doi:10.1016/j.isci.2026.117032
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- Authors
- Phan, T., Rowland, R. A., Ponticorvo, A., Le, B. C., Sharif, S. A., Kennedy, G. T., Wilson, R. H., & Durkin, A. J.
- Affiliations
- University of California, Irvine (US)
- Corresponding author
- Phan, Thinh
- Venue
- Journal of Biomedical Optics
- Publisher
- SPIE-Intl Soc Optical Eng
- Published
- 2022-03-23
- Volume
- 27
- Issue
- 03
- Pages
- 1-14
- ISSN
- 1083-3668 (ISSN-L)
- 1560-2281
- PMC
- PMC8942554
- OpenAlex
- W4221041716
- Cited by
- 18
- References
- 31
- Citations by year
- 2026: 7 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 1
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Biomedical Optics (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- eScholarship (California Digital Library) (submittedVersion, cc-by)
- Funders
- National Institute of Biomedical Imaging and Bioengineering (R21AG06600, P41EB015890, R01GM108634)
- National Institute of General Medical Sciences (2R01GM108634-05A1)
- Air Force Office of Scientific Research (FA9550-20-1-0052)
- National Institutes of Health (P41EB015890, R01GM108634)
- NIH (P41EB015890)
- NIH/NIBIB (P41EB015890)
- Beckman Foundation and the NIH (P41EB015890)
- NIH (NIBIB) Laser Microbeam and Medical Program (P41EB015890)
- NIBIB Biomedical Technology Research Center LAMMP (P41EB015890)
- NIH NIBIB Biomedical Technology Research Center LAMMP (P41EB015890)
- NIH/NIBIB funded LAMMP (P41EB015890)
- US National Institutes of Health (P41EB015890)
- Arnold & Mabel Beckman Foundation (P41EB015890)
- National Institute of Biomedical Imaging Bioengineering (P41EB015890)
- NIH NIBIB (P41EB015890)
- NIH/NIGMS (R01GM108634)
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
- Health Sciences › Medicine › Oncology › Cutaneous Melanoma Detection and Management
- Physical Sciences › Engineering › Biomedical Engineering › Optical Coherence Tomography Applications
- Keywords
- skin pigmentation, epidermal melanin, spatial frequency domain imaging, diffuse optical imaging, reduced scattering coefficient, absorption coefficient, tristimulus colorimetry, Fitzpatrick skin type, wavelength dependence
- MeSH terms
- Colorimetry, Epidermis, Humans, Skin, Skin Pigmentation, Optical Imaging
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