Signaling Responses After Varying Sequencing of Strength and Endurance Training in a Fed State

Abstract

Purpose: To compare anabolic signaling responses to differing sequences of concurrent strength and endurance training in a fed state. Methods: Eighteen resistance-trained men were randomly assigned to the following experimental conditions: strength training (ST), strength followed by endurance training (ST-END), or endurance followed by strength training (END-ST). Muscle tissue samples were taken from the vastus lateralis before each exercise protocol, on cessation of exercise, and 1 h after cessation of strength training. Tissue was analyzed for total and phosphorylated (p-) signaling proteins linked to the mTOR and AMPK networks. Results: Strength-training performance was similar between ST, ST-END, and END-ST. p-S6k1 was elevated from baseline 1 h posttraining in ST and ST-END (both P < .05). p-4E-BP1 was significantly lower than baseline post-ST ( P = .01), whereas at 1 h postexercise in the ST-END condition p-4E-BP1 was significantly greater than postexercise ( P = .04). p-ACC was elevated from baseline both postexercise and 1 h postexercise (both P < .05) in the END-ST condition. AMPK, mTOR, p38, PKB, and eEF2 responded similarly to ST, ST-END, and END-ST. Signaling responses to ST, ST-END, and END were largely similar. As such it cannot be ascertained which sequence of concurrent strength and endurance training is most favorable in promoting anabolic signaling. Conclusions: In the case of the current study an acute bout of concurrent training of differing sequences elicited similar responses of the AMPK and mTOR networks.