Early Time Course of Akt Phosphorylation after Endurance and Resistance Exercise
Abstract
PURPOSE: The aim of this study was to determine the early time course of exercise-induced signaling after divergent contractile activity associated with resistance and endurance exercise. METHODS: Sixteen male subjects were randomly assigned to either a cycling (CYC; n = 8, 60 min, 70% V˙O2peak) or resistance (REX; n = 8, 8 x 5 leg extension, 80% one-repetition maximum, 3-min recovery) exercise group. Serial muscle biopsies were obtained from vastus lateralis at rest before, immediately after, and after 15, 30, and 60 min of passive recovery to determine early signaling responses after exercise. RESULTS: There were comparable increases from rest in Akt(Thr308/Ser473) and mTOR(Ser2448) phosphorylation during the postexercise time course that peaked 30-60 min after both CYC and REX (P < 0.05). There were also similar patterns in p70S6K(Thr389) and 4E-BP1(Thr37/46) phosphorylation, but a greater magnitude of effect was observed for REX and CYC, respectively (P < 0.05). However, AMPK(Thr172) phosphorylation was only significantly elevated after CYC (P < 0.05), and we observed divergent responses for glycogen synthase(Ser641) and AS160 phosphorylation that were enhanced after CYC but not REX (P < 0.05). CONCLUSIONS: We show a similar time course for Akt-mTOR-S6K phosphorylation during the initial 60-min recovery period after divergent contractile stimuli. Conversely, enhanced phosphorylation status of proteins that promote glucose transport and glycogen synthesis only occurred after endurance exercise. Our results indicate that endurance and resistance exercise initiate translational signaling, but high-load, low-repetition contractile activity failed to promote phosphorylation of pathways regulating glucose metabolism.
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The peak elevation in signalling proteins pivotal for increasing initiation of translation of muscle proteins appears to peak between 30 and 60 min after exercise.
“the peak elevation in signalling proteins shown to be pivotal for increasing the initiation of translation of muscle proteins, which for the most part appears to peak between 30 and 60 min after exercise [71].”
From International Society of Sports Nutrition Position Stand: protein and exercise (2017) · cites this paper as [71]
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- Authors
- CAMERA, D. M., EDGE, J., SHORT, M. J., HAWLEY, J. A., & COFFEY, V. G.
- Affiliations
- RMIT University (AU)
- University of Auckland (NZ)
- Massey University (NZ)
- Venue
- Medicine & Science in Sports & Exercise
- Published
- 2010-10
- Volume
- 42
- Issue
- 10
- Pages
- 1843-1852
- ISSN
- 0195-9131 (ISSN-L)
- OpenAlex
- W2027102393
- Cited by
- 154
- References
- 38
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 5 · 2023: 6 · 2022: 16 · 2021: 6 · 2020: 6 · 2019: 4 · 2018: 14 · 2017: 9 · 2016: 14 · 2015: 9 · 2014: 17 · 2013: 15 · 2012: 11
- FWCI
- 5.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Medicine & Science in Sports & Exercise (publishedVersion)
- PubMed (publishedVersion)
- Research Bank (Australian Catholic University) (submittedVersion)
- RMIT Research Repository (RMIT University Library) (submittedVersion)
- Figshare (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- Akt phosphorylation, mTOR signaling, p70S6K, 4E-BP1, AMPK, glycogen synthase, AS160, endurance exercise, resistance training, post-exercise recovery, vastus lateralis, glucose transport
- MeSH terms
- Adult, Blood Glucose, Glycogen Synthase, Humans, Insulin, Male, Muscle Contraction, Phosphoproteins, Phosphorylation, Physical Endurance, Muscle, Skeletal, Cell Cycle Proteins, Lactic Acid, GTPase-Activating Proteins, Ribosomal Protein S6 Kinases, 70-kDa, Adaptor Proteins, Signal Transducing, Proto-Oncogene Proteins c-akt, Resistance Training, AMP-Activated Protein Kinases, TOR Serine-Threonine Kinases
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