The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins
Abstract
An ultrasensitive method for detecting protein analytes has been developed. The system relies on magnetic microparticle probes with antibodies that specifically bind a target of interest [prostate-specific antigen (PSA) in this case] and nanoparticle probes that are encoded with DNA that is unique to the protein target of interest and antibodies that can sandwich the target captured by the microparticle probes. Magnetic separation of the complexed probes and target followed by dehybridization of the oligonucleotides on the nanoparticle probe surface allows the determination of the presence of the target protein by identifying the oligonucleotide sequence released from the nanoparticle probe. Because the nanoparticle probe carries with it a large number of oligonucleotides per protein binding event, there is substantial amplification and PSA can be detected at 30 attomolar concentration. Alternatively, a polymerase chain reaction on the oligonucleotide bar codes can boost the sensitivity to 3 attomolar. Comparable clinically accepted conventional assays for detecting the same target have sensitivity limits of ∼3 picomdar, six orders of magnitude less sensitive than what is observed with this method.
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Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
A DNA-based method for rationally assembling nanoparticles into macroscopic materials
Mirkin, C. A., Letsinger, R. L., Mucic, R. C., & Storhoff, J. J. (1996) · Nature · doi:10.1038/382607a0 · Cited by 6,683
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Cao, Y. C., Jin, R., & Mirkin, C. A. (2002) · Science · doi:10.1126/science.297.5586.1536 · Cited by 2,971
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MacBeath, G., & Schreiber, S. L. (2000) · Science · doi:10.1126/science.289.5485.1760 · Cited by 2,767
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
Bustin, S. (2002) · Journal of Molecular Endocrinology · doi:10.1677/jme.0.0290023 · Cited by 2,546
Scanometric DNA Array Detection with Nanoparticle Probes
Taton, T. A., Mirkin, C. A., & Letsinger, R. L. (2000) · Science · doi:10.1126/science.289.5485.1757 · Cited by 2,411
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Zhu, H., Bilgin, M., Bangham, R., Hall, D., Casamayor, A., Bertone, P., Lan, N., Jansen, R., Bidlingmaier, S., Houfek, T., et al. (2001) · Science · doi:10.1126/science.1062191 · Cited by 1,884
Demers, L. M., Mirkin, C. A., Mucic, R. C., Reynolds, R. A., Letsinger, R. L., Elghanian, R., & Viswanadham, G. (2000) · Analytical Chemistry · doi:10.1021/ac0006627 · Cited by 1,109
Self-citationHaab, B. B., Dunham, M. J., & Brown, P. O. (2001) · Genome Biology · doi:10.1186/gb-2001-2-2-research0004 · Cited by 892
Immuno-PCR: Very Sensitive Antigen Detection by Means of Specific Antibody-DNA Conjugates
Sano, T., Smith, C. L., & Cantor, C. R. (1992) · Science · doi:10.1126/science.1439758 · Cited by 861
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Metadata
- Authors
- Nam, J.-M., Thaxton, C. S., & Mirkin, C. A.
- Venue
- Science
- Published
- 2003-09-26
- Volume
- 301
- Issue
- 5641
- Pages
- 1884-1886
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1971428641
- Cited by
- 2,390
- References
- 16
- FWCI
- 30.24
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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