The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Heterochromatic Silencing and HP1 Localization in Drosophila Are Dependent on the RNAi Machinery
Abstract
Genes normally resident in euchromatic domains are silenced when packaged into heterochromatin, as exemplified in Drosophila melanogaster by position effect variegation (PEV). Loss-of-function mutations resulting in suppression of PEV have identified critical components of heterochromatin, including proteins HP1, HP2, and histone H3 lysine 9 methyltransferase. Here, we demonstrate that this silencing is dependent on the RNA interference machinery, using tandem mini-white arrays and white transgenes in heterochromatin to show loss of silencing as a result of mutations in piwi, aubergine , or spindle-E ( homeless ), which encode RNAi components. These mutations result in reduction of H3 Lys 9 methylation and delocalization of HP1 and HP2, most dramatically in spindle-E mutants.
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Cites
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Role of MicroRNAs in Plant and Animal Development
Carrington, J. C., & Ambros, V. (2003) · Science · doi:10.1126/science.1085242 · Cited by 1,767
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Establishment and Maintenance of a Heterochromatin Domain
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ARGONAUTE4 Control of Locus-Specific siRNA Accumulation and DNA and Histone Methylation
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Post-transcriptional gene silencing by double-stranded RNA
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Cited by
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Esteller, M. (2011) · Nature Reviews Genetics · doi:10.1038/nrg3074 · Cited by 4,825
Small silencing RNAs: an expanding universe
Ghildiyal, M., & Zamore, P. D. (2009) · Nature Reviews Genetics · doi:10.1038/nrg2504 · Cited by 2,325
Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs
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A germline-specific class of small RNAs binds mammalian Piwi proteins
Girard, A., Sachidanandam, R., Hannon, G. J., & Carmell, M. A. (2006) · Nature · doi:10.1038/nature04917 · Cited by 1,762
PIWI-interacting small RNAs: the vanguard of genome defence
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Metadata
- Authors
- Pal-Bhadra, M., Leibovitch, B. A., Gandhi, S. G., Rao, M., Bhadra, U., Birchler, J. A., & Elgin, S. C. R.
- Venue
- Science
- Published
- 2004-01-30
- Volume
- 303
- Issue
- 5658
- Pages
- 669-672
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2146865647
- Cited by
- 656
- References
- 20
- FWCI
- 30.94
- Type
- journal article
- Language
- en
- Open access
- closed
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- Pal-Bhadra, M., Leibovitch, B. A., Gandhi, S. G., Rao, M., Bhadra, U., Birchler, J. A., & Elgin, S. C. R. (2004). Heterochromatic Silencing and HP1 Localization in Drosophila Are Dependent on the RNAi Machinery. Science. https://doi.org/10.1126/science.1092653
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