The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Attomole Protein Characterization by Capillary Electrophoresis-Mass Spectrometry
Abstract
Electrospray ionization with an ultralow flow rate (≤4 nanoliters per minute) was used to directly couple capillary electrophoresis with tandem mass spectrometry for the analysis and identification of biomolecules in mixtures. A Fourier transform mass spectrometer provided full spectra (>30 kilodaltons) at a resolving power of ≈60,000 for injections of 0.7 × 10 −18 to 3 × 10 −18 mole of 8- to 29-kilodalton proteins with errors of <1 dalton in molecular mass. Using a crude isolate from human blood, a value of 28,780.6 daltons (calculated, 28,780.4 daltons) was measured for carbonic anhydrase, representing 1 percent by weight of the protein in a single red blood cell. Dissociation of molecular ions from 9 × 10 −18 mole of carbonic anhydrase gave nine sequence-specific fragment ions, more data than required for unique retrieval of this enzyme from the protein database.
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Cites
Electrospray Ionization for Mass Spectrometry of Large Biomolecules
Fenn, J. B., Mann, M., Meng, C. K., Wong, S. F., & Whitehouse, C. M. (1989) · Science · doi:10.1126/science.2675315 · Cited by 7,634
Roepstorff, P., & Fohlman, J. (1984) · Biological Mass Spectrometry · doi:10.1002/bms.1200111109 · Cited by 2,633
Error-Tolerant Identification of Peptides in Sequence Databases by Peptide Sequence Tags
Mann, M., & Wilm, M. (1994) · Analytical Chemistry · doi:10.1021/ac00096a002 · Cited by 1,468
Capillary Zone Electrophoresis
Jorgenson, J. W., & Lukacs, K. D. (1983) · Science · doi:10.1126/science.6623076 · Cited by 1,289
Electrospray and Taylor-Cone theory, Dole's beam of macromolecules at last?
Wilm, M. S., & Mann, M. (1994) · International Journal of Mass Spectrometry and Ion Processes · doi:10.1016/0168-1176(94)04024-9 · Cited by 974
Interpreting mass spectra of multiply charged ions
Mann, M., Meng, C. K., & Fenn, J. B. (1989) · Analytical Chemistry · doi:10.1021/ac00190a023 · Cited by 588
Micro-electrospray mass spectrometry: Ultra-high-sensitivity analysis of peptides and proteins
Emmett, M. R., & Caprioli, R. M. (1994) · Journal of the American Society for Mass Spectrometry · doi:10.1016/1044-0305(94)85001-1 · Cited by 510
Curl, R. F., & Smalley, R. E. (1988) · Science · doi:10.1126/science.242.4881.1017 · Cited by 469
Senko, M. W., Beu, S. C., & McLaffertycor, F. W. (1995) · Journal of the American Society for Mass Spectrometry · doi:10.1016/1044-0305(95)00017-8 · Cited by 451
Purification and properties of bovine erythrocyte carbonic anhydrase
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Cited by
Mass spectrometry-based proteomics
Aebersold, R., & Mann, M. (2003) · Nature · doi:10.1038/nature01511 · Cited by 6,955
Electron Capture Dissociation of Multiply Charged Protein Cations. A Nonergodic Process
Zubarev, R. A., Kelleher, N. L., & McLafferty, F. W. (1998) · Journal of the American Chemical Society · doi:10.1021/ja973478k · Cited by 1,881
Self-citationProgress in Top-Down Proteomics and the Analysis of Proteoforms
Toby, T. K., Fornelli, L., & Kelleher, N. L. (2016) · Annual Review of Analytical Chemistry · doi:10.1146/annurev-anchem-071015-041550 · Cited by 576
Self-citationSenko, M. W., Hendrickson, C. L., Emmett, M. R., Shi, S. D. H., & Marshall, A. G. (1997) · Journal of the American Society for Mass Spectrometry · doi:10.1016/s1044-0305(97)00126-8 · Cited by 462
Lombard‐Banek, C., Moody, S. A., & Nemes, P. (2016) · Angewandte Chemie International Edition · doi:10.1002/anie.201510411 · Cited by 214
Metadata
- Authors
- Valaskovic, G. A., Kelleher, N. L., & McLafferty, F. W.
- Venue
- Science
- Published
- 1996-08-30
- Volume
- 273
- Issue
- 5279
- Pages
- 1199-1202
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1968746767
- Cited by
- 398
- References
- 25
- FWCI
- 23.50
- Type
- journal article
- Language
- en
- Open access
- closed
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- Valaskovic, G. A., Kelleher, N. L., & McLafferty, F. W. (1996). Attomole Protein Characterization by Capillary Electrophoresis-Mass Spectrometry. Science. https://doi.org/10.1126/science.273.5279.1199
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
