Cited by 3,544T-cell and B-cell ImmunologyImmunotherapy and Immune Responsesvaccines and immunoinformatics approaches

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Phenotypic Analysis of Antigen-Specific T Lymphocytes

Altman, John D., Moss, Paul A. H., Goulder, Philip J. R., Barouch, Dan H., McHeyzer-Williams, Michael G., Bell, John I., McMichael, Andrew J., Davis, Mark M.·1996·Science

DOI 10.1126/science.274.5284.94

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Abstract

Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays. This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens.

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Handbook of Experimental Immunology.
Denman, A. (1968) · Annals of Internal Medicine · doi:10.7326/0003-4819-68-6-1376_1 · Cited by 2,414
HANDBOOK OF EXPERIMENTAL IMMUNOLOGY
Holborow, E. J. (1968) · Journal of Clinical Pathology · doi:10.1136/jcp.21.4.534-c · Cited by 678
HLA-A2-peptide complexes: refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigenic peptides.
Garboczi, D. N., Hung, D. T., & Wiley, D. C. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.8.3429 · Cited by 671
Use of Peptide Libraries to Map the Substrate Specificity of a Peptide-Modifying Enzyme: A 13 Residue Consensus Peptide Specifies Biotinylation in Escherichia coli
Schatz, P. J. (1993) · Nature Biotechnology · doi:10.1038/nbt1093-1138 · Cited by 585
Antigen-Specific Development of Primary and Memory T Cells in Vivo
McHeyzer‐Williams, M. G., & Davis, M. M. (1995) · Science · doi:10.1126/science.7535476 · Cited by 439
Self-citation
Ia determinants on stimulated human T lymphocytes. Occurrence on mitogen- and antigen-activated T cells.
Ko, H. S., Fu, S. M., WINCHESTER, R. J., Yu, D. T. Y., & Kunkel, H. G. (1979) · The Journal of Experimental Medicine · doi:10.1084/jem.150.2.246 · Cited by 439
HIV-1 gag-specific cytotoxic T lymphocytes defined with recombinant vaccinia virus and synthetic peptides
Nixon, D. F., Townsend, A., Elvin, J. G., Rizza, C. R., Gallwey, J., & McMichael, A. J. (1988) · Nature · doi:10.1038/336484a0 · Cited by 431
Self-citation
Kinetics of T-cell receptor binding to peptide/I-Ek complexes: correlation of the dissociation rate with T-cell responsiveness.
Matsui, K., Boniface, J. J., Steffner, P., Reay, P. A., & Davis, M. M. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.26.12862 · Cited by 425
Self-citation
Quantitative analysis of the human immunodeficiency virus type 1 (HIV-1)-specific cytotoxic T lymphocyte (CTL) response at different stages of HIV-1 infection: differential CTL responses to HIV-1 and Epstein-Barr virus in late disease.
Carmichael, A. J., Jin, X., Sissons, P., & Borysiewicz, L. K. (1993) · The Journal of Experimental Medicine · doi:10.1084/jem.177.2.249 · Cited by 418
Unusually high frequencies of HIV-specific cytotoxic T lymphocytes in humans.
Hoffenbach, A., Langlade‐Demoyen, P., Dadaglio, G., VILMER, E., Michel, F., Mayaud, C. M., Autran, B., & Plata, F. (1989) · The Journal of Immunology · doi:10.4049/jimmunol.142.2.452 · Cited by 304

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Hayakawa K., Ishii R., Yamasaki K., Kishimoto T., Hardy R. R., ibid. 84, 1379 (1987);
Schatz P. J., Biotechnology 11, 1138 (1993).
Johnson R. P., et al., ibid. 147, 1512 (1991).
Koup R. A., et al., ibid. 174, 1593 (1991).
Tripp R. A., Hou S., Doherty P. C., ibid. 154, 5870 (1995);

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“pMHC tetramers are comprised of the actual ligands recognized by the TCRs of epitope-specific T cells, so in theory they should bind all epitope-specific T cells within a tissue sampl”
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Sorafenib, but not sunitinib, affects function of dendritic cells and induction of primary immune responses
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Lee, K.-H., Wang, E., Nielsen, M.-B., Wunderlich, J. R., Migueles, S. A., Connors, M., Steinberg, S. M., Rosenberg, S. A., & Marincola, F. M. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.11.6292 · Cited by 285
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Impaired function of circulating HIV-specific CD8+ T cells in chronic human immunodeficiency virus infection
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“The phenoytpe of T cells recovered from our model is very similar to that of cells that have been activated in vivo in response to Ag (24, 39, 40).”
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“Tetramers were generated, as previously described (32, 33).”
Method
What is Good’s syndrome? Immunological abnormalities in patients with thymoma
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Understanding the drivers of MHC restriction of T cell receptors
Gruta, N. L. L., Gras, S., Daley, S. R., Thomas, P. G., & Rossjohn, J. (2018) · Nature reviews. Immunology · doi:10.1038/s41577-018-0007-5 · Cited by 283
Immunotherapy through TCR gene transfer
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Autoreactive CD8 T cells associated with β cell destruction in type 1 diabetes
Pinkse, G. G. M., Tysma, O. M. H., van Bergen, C. A. M., Kester, M. G. D., OSSENDORP, F. A., van Veelen, P. A., Keymeulen, B., Pipeleers, D., Drijfhout, J. W., & Roep, B. O. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0508621102 · Cited by 282
Tetramer-based quantification of cytomegalovirus (CMV)–specific CD8+ T lymphocytes in T-cell–depleted stem cell grafts and after transplantation may identify patients at risk for progressive CMV infection
Gratama, J. W., van Esser, J. W. J., Lamers, C. H. J., Tournay, C., Löwenberg, B., Bolhuis, R. L. H., & Cornelissen, J. J. (2001) · Blood · doi:10.1182/blood.v98.5.1358 · Cited by 277
Cytomegalovirus (CMV) Phosphoprotein 65 Makes a Large Contribution to Shaping the T Cell Repertoire in CMV‐Exposed Individuals
Kern, F., Bunde, T., Faulhaber, N., Kiecker, F., Khatamzas, E., Rudawski, I., Prüß, A., Gratama, J.-W. C., Volkmer, R., Ewert, R., et al. (2002) · The Journal of Infectious Diseases · doi:10.1086/340637 · Cited by 277
Abundant Tax protein expression in CD4+ T cells infected with human T-cell lymphotropic virus type I (HTLV-I) is prevented by cytotoxic T lymphocytes
Hanon, E., Hall, S., Taylor, G. P., Saito, M., Davis, R., Tanaka, Y., Usuku, K., Osame, M., Weber, J. N., & Bangham, C. R. M. (2000) · Blood · doi:10.1182/blood.v95.4.1386.004k22_1386_1392 · Cited by 276
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“tetramers were prepared as previously described (3) and included tetramers”
Method
Attrition of T Cell Memory
Selin, L. K., Lin, M. Y., Kraemer, K. A., Pardoll, D. M., Schneck, J. P., Varga, S. M., Santolucito, P. A., Pinto, A. K., & Welsh, R. M. (1999) · Immunity · doi:10.1016/s1074-7613(00)80147-8 · Cited by 275
T-cell-receptor gene therapy
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“Because TCR-modified T cells can readily be visualized with MHC TETRAMERS”
Background
Microbiota-Induced Type I Interferons Instruct a Poised Basal State of Dendritic Cells
Schaupp, L., Muth, S., Rogell, L., Kofoed-Branzk, M., Melchior, F., Lienenklaus, S., Ganal-Vonarburg, S. C., Klein, M., Guendel, F., Hain, T., et al. (2020) · Cell · doi:10.1016/j.cell.2020.04.022 · Cited by 263
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“PE- or allophycocyanin-labeled M27-MHC-tetramer and G154-MHC-tetramers were produced in the immune monitoring laboratory at the Fred Hutchinson Cancer Center based on previously described protocols (14).”
Method
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Method
Frequency of Class I HLA-Restricted Anti-HIV CD8+ T Cells in Individuals Receiving Highly Active Antiretroviral Therapy (HAART)
Gray, C. M., Lawrence, J., Schapiro, J., Altman, J. D., Winters, M. A., Crompton, M., Loi, M., Kundu‐Raychaudhuri, S., Davis, M. M., & Merigan, T. C. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.162.3.1780 · Cited by 270
“With the introduction of peptide/MHC tetrameric complexes (5), it became apparent that the frequency of Ag-specific cells was consistently higher than that shown by limiting dilution analysis.”
BackgroundMethodInfluentialSelf-citation
Critical Role for Cd8 in T Cell Receptor Binding and Activation by Peptide/Major Histocompatibility Complex Multimers
Daniëls, M. A., & Jameson, S. C. (2000) · The Journal of Experimental Medicine · doi:10.1084/jem.191.2.335 · Cited by 269
“Using standard procedures, K b /peptide multimeric complexes were synthesized bearing each of these peptides (16, 18).”
BackgroundMethod
Maintenance of Large Numbers of Virus-Specific CD8+ T Cells in HIV-Infected Progressors and Long-Term Nonprogressors
Gea‐Banacloche, J., Migueles, S. A., Martino, L., Shupert, W. L., McNeil, A. C., Sabbaghian, M. S., Ehler, L. A., Prussin, C., Stevens, R. A., Lambert, L., et al. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.2.1082 · Cited by 267
“cells specific for a given peptide (13).”
ResultSelf-citation
Immediate Cytotoxicity But Not Degranulation Distinguishes Effector and Memory Subsets of CD8+ T Cells
Wolint, P., Betts, M. R., Koup, R. A., & Oxenius, A. (2004) · The Journal of Experimental Medicine · doi:10.1084/jem.20031799 · Cited by 262
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Kuroda, M. J., Schmitz, J. E., Charini, W. A., Nickerson, C. E., Lifton, M. A., Lord, C. I., Forman, M. A., & Letvin, N. L. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.162.9.5127 · Cited by 265
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Chen, W., Norbury, C. C., Cho, Y., Yewdell, J. W., & Bennink, J. R. (2001) · The Journal of Experimental Medicine · doi:10.1084/jem.193.11.1319 · Cited by 264
“The rekindled interest is also due to technological advances in T cell quantitation, such as the introduction of intracellular cytokine staining (ICS; reference 13) and MHC-peptide tetramers (14).”
Background
Tetrameric HLA class I–minor histocompatibility antigen peptide complexes demonstrate minor histocompatibility antigen-specific cytotoxic T lymphocytes in patients with graft-versus-host disease
Mutis, T., Gillespie, G. M. A., Schrama, E., Falkenburg, J. H. F., Moss, P. A. H., & Goulmy, E. (1999) · Nature Medicine · doi:10.1038/10563 · Cited by 264
“Folded monomeric complexes were biotinylated using BirA enzyme and were then purified by gel filtration and ion exchange as describe”
MethodSelf-citation
An inverse relationship between T cell receptor affinity and antigen dose during CD4 + T cell responses in vivo and in vitro
Rees, W. A., Bender, J., Teague, T. K., Kedl, R. M., Crawford, F., Marrack, P., & Kappler, J. W. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.17.9781 · Cited by 263
A New Look at T Cells
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Self-citation
Analysis of Gag-specific Cytotoxic T Lymphocytes in Simian Immunodeficiency Virus–infected Rhesus Monkeys by Cell Staining with a Tetrameric Major Histocompatibility Complex Class I–Peptide Complex
Kuroda, M. J., Schmitz, J. E., Barouch, D. H., Craiu, A., Allen, T. M., Sette, A., Watkins, D. I., Forman, M. A., & Letvin, N. L. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.187.9.1373 · Cited by 262
“who described a homogeneous phenotypic memory type profile (CD45RO1 and CD62L2) for their MHC class I–peptide binding cells (1).”
BackgroundInfluentialSelf-citation
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“Developments in the identification of antigen-specific T cell response”
Background
Determinant spreading associated with clinical response in dendritic cell-based immunotherapy for malignant melanoma.
Butterfield, L. H., Ribas, A., Dissette, V. B., Amarnani, S. N., Vu, H. T., Oseguera, D. K., Wang, H.-J., Elashoff, R. M., McBride, W. H., Mukherji, B., et al. (2003) · PubMed · Cited by 255
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Mongkolsapaya, J., Duangchinda, T., Dejnirattisai, W., Vasanawathana, S., Avirutnan, P., Jairungsri, A., Khemnu, N., Tangthawornchaikul, N., Chotiyarnwong, P., Sae-Jang, K., et al. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.176.6.3821 · Cited by 253
“Tetramers were prepared as described previously (15).”
Self-citation
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Method
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“Extracellular antibody-induced clustering Valitutti et al. (1995) observed that at low antigen densi-of TCR complexes (Bekoff et al., 1986; Janeway, 1995) ties, single MHC-peptide complexes serially engagedor of chimeric constructs carrying TCR cytoplasmic domultiple TCR molecules, using…”
Spontaneous cytotoxic T-cell responses against survivin-derived MHC class I-restricted T-cell epitopes in situ as well as ex vivo in cancer patients.
Andersen, M. H., Pedersen, L. Ø., Capeller, B., Bröcker, E. B., Becker, J. C., & Straten, P. T. (2001) · PubMed · Cited by 255
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Self-citation
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Leishman, A. J., Naidenko, O. V., Attinger, A., Koning, F., Lena, C. J., Xiong, Y., Chang, H., Reinherz, E. L., Kronenberg, M., & Cheroutre, H. (2001) · Science · doi:10.1126/science.1063564 · Cited by 255
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“Soluble HLA-A2 tetramers were prepared with immunizing peptides and 2-microglobulin as described (25), conjugated to phycoerythrin, and validated using peptide-specific CTLs as described (14).”
Method
A Previously Unrecognized H-2Db-Restricted Peptide Prominent in the Primary Influenza A Virus-Specific CD8+T-Cell Response Is Much Less Apparent following Secondary Challenge
Belz, G. T., Xie, W., Altman, J. D., & Doherty, P. C. (2000) · Journal of Virology · doi:10.1128/jvi.74.8.3486-3493.2000 · Cited by 253
“Virus-specific CD8(1) T cells were identified using tetrameric complexes of the H-2D glycoprotein and peptides (1) derived from the NP ASNENMETM (23) and PA SSLENFRAYV,”
BackgroundSelf-citation
Direct evidence that leukemic cells present HLA-associated immunogenic peptides derived from the BCR-ABL b3a2 fusion protein
Clark, R. E., Dodi, I. A., Hill, S. C., Lill, J. R., Aubert, G., Macintyre, A. R., Rojas, J. M., Bourdon, A., Bonner, P. L. R., Wang, L., et al. (2001) · Blood · doi:10.1182/blood.v98.10.2887 · Cited by 253
IL-4-secreting CD4+ T cells are crucial to the development of CD8+ T-cell responses against malaria liver stages
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“SYVPSAEQI-specific H2K d tetramer was obtained from the NIH Tetramer Facility or prepared as describe”
Method
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Ebert, P. J. R., Jiang, S., Xie, J., Li, Q.-J., & Davis, M. M. (2009) · Nature Immunology · doi:10.1038/ni.1797 · Cited by 251
“ATPase–I-E k and GP–I-E k monomers were prepared, biotinylated and made into tetramers as describe”
Self-citation
Induction of AIDS Virus-Specific CTL Activity in Fresh, Unstimulated Peripheral Blood Lymphocytes from Rhesus Macaques Vaccinated with a DNA Prime/Modified Vaccinia Virus Ankara Boost Regimen
Allen, T. M., Vogel, T. U., FULLER, D. H., Mothé, B. R., Steffen, S. R., Boyson, J. E., Shipley, T., Fuller, J., Hanke, T., Sette, A., et al. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.164.9.4968 · Cited by 251
“Once the PCR products were cloned into an expression vector using NdeI and BamHI cloning sites (52), the rhesus MHC molecules were expressed and then folded with human b2m and peptide.”
MethodSelf-citation
T-Scan: A Genome-wide Method for the Systematic Discovery of T Cell Epitopes
Kula, T., Dezfulian, M. H., Wang, C. I., Abdelfattah, N. S., Hartman, Z. C., Wucherpfennig, K. W., Lyerly, H. K., & Elledge, S. J. (2019) · Cell · doi:10.1016/j.cell.2019.07.009 · Cited by 250
“Classic approaches for understanding T cell specificity rely on readouts of T cell function, which include assays for cytotoxicity, cytokine release, and proliferation in the presence of candidate antigens (Sharma and Holt, 2014), augmented by peptide-MHC tetramers for antigen-specific populations (Altman et al., 1996…”
Background
Direct visualization of antigen-specific T cells: HTLV-1 Tax11–19- specific CD8 + T cells are activated in peripheral blood and accumulate in cerebrospinal fluid from HAM/TSP patients
Greten, T. F., Slansky, J. E., Kubota, R., Soldan, S. S., Jaffee, E. M., Leist, T. P., Pardoll, D. M., Jacobson, S., & Schneck, J. P. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.13.7568 · Cited by 247
Effects of Interleukin-12 on the Immune Response to a Multipeptide Vaccine for Resected Metastatic Melanoma
Lee, P. P., Wang, F., Kuniyoshi, J., Rubio, V., Stuge, T. B., Groshen, S. G., Gee, C., Lau, R., Jeffery, G., Margolin, K. A., et al. (2001) · Journal of Clinical Oncology · doi:10.1200/jco.2001.19.18.3836 · Cited by 245
Immunology of hepatitis B infection
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Cytomegalovirus Infection: A Driving Force in Human T Cell Immunosenescence
KOCH, S., LARBI, A., ÖZCELIK, D., SOLANA, R., GOUTTEFANGEAS, C., ATTIG, S., WIKBY, A., STRINDHALL, J., FRANCESCHI, C., & PAWELEC, G. (2007) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1396.043 · Cited by 242
Large Numbers of Dysfunctional CD8+ T Lymphocytes Bearing Receptors for a Single Dominant CMV Epitope in the Very Old
Ouyang, Q., Wagner, W. M., Wikby, A., Walter, S., Aubert, G., Dodi, A. I., Travers, P. J., & Pawelec, G. (2003) · Journal of Clinical Immunology · doi:10.1023/a:1024580531705 · Cited by 239
“HLA class I/peptide tetramers were synthesized as described previously by others ( 18 ).”
Accelerated immune senescence and HIV-1 infection
APPAY, V, ALMEIDA, J., SAUCE, D., AUTRAN, B., & PAPAGNO, L. (2007) · Experimental Gerontology · doi:10.1016/j.exger.2006.12.003 · Cited by 236
Age-associated accumulation of CMV-specific CD8+ T cells expressing the inhibitory killer cell lectin-like receptor G1 (KLRG1)
Ouyang, Q., Wagner, W. M., Voehringer, D., Wikby, A., Klatt, T., Walter, S., Müller, C. A., Pircher, H., & Pawelec, G. (2003) · Experimental Gerontology · doi:10.1016/s0531-5565(03)00134-7 · Cited by 238
T cell receptor αβ diversity inversely correlates with pathogen-specific antibody levels in human cytomegalovirus infection
Wang, G. C., Dash, P., McCullers, J. A., Doherty, P. C., & Thomas, P. G. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3003647 · Cited by 238
Antigen-Antibody Immune Complexes Empower Dendritic Cells to Efficiently Prime Specific CD8+ CTL Responses In Vivo
Schuurhuis, D. H., Ioan‐Facsinay, A., Nagelkerken, B., Schip, J. J. V. D. P.-V., Sedlik, C., Melief, C. J. M., Verbeek, J. S., & OSSENDORP, F. A. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.5.2240 · Cited by 238
Recognition of the Class Ib Molecule Qa-1b by Putative Activating Receptors Cd94/Nkg2c and Cd94/Nkg2e on Mouse Natural Killer Cells
Vance, R. E., Jamieson, A. M., & Raulet, D. H. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.190.12.1801 · Cited by 238
“1804 Recognition of Qa-1 b by CD94/NKG2C and CD94/NKG2E were generated and used as described previously (9, 39), except”
Method
Directed evolution of a stable scaffold for T-cell receptor engineering
Shusta, E. V., Holler, P. D., Kieke, M. C., Kranz, D. M., & Wittrup, K. D. (2000) · Nature Biotechnology · doi:10.1038/77325 · Cited by 237
“In an enzyme-linked immunosorbent assay (ELISA)-based multivalent pMH”
Method
On the Composition of the Preimmune Repertoire of T Cells Specific for Peptide–Major Histocompatibility Complex Ligands
Jenkins, M. K., Chu, H., McLachlan, J. B., & Moon, J. J. (2010) · Annual Review of Immunology · doi:10.1146/annurev-immunol-030409-101253 · Cited by 256
Identification of MAGE-3 Epitopes Presented by HLA-DR Molecules to CD4+ T Lymphocytes
Chaux, P., Vantomme, V., Stroobant, V., Thielemans, K., Corthals, J., Luiten, R. M., Eggermont, A. M. M., Boon, T., & van der Bruggen, P. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.189.5.767 · Cited by 234
“Another possibility, which narrows the analysis to certain epitopes, is the use of a set of relevant peptides that can be used either to select and amplify peptide-specific T cells in vitro or to label directly T cell receptors with soluble HLA tetramers presenting the relevant peptide (50, 51).”
Background
Loss of CD127 Expression Defines an Expansion of Effector CD8+ T Cells in HIV-Infected Individuals
Paiardini, M., Cervasi, B., Albrecht, H., Muthukumar, A., Dunham, R. M., Gordon, S. N., Radziewicz, H., Piedimonte, G., Magnani, M., Montroni, M., et al. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.174.5.2900 · Cited by 233
“Tetrameric HLA class I molecules were produced as previously described (25).”
MethodSelf-citation
Prediction of spontaneous autoimmune diabetes in NOD mice by quantification of autoreactive T cells in peripheral blood
Trudeau, J. D., Kelly-Smith, C., Verchere, C. B., Elliott, J. F., Dutz, J. P., Finegood, D. T., Santamaría, P., & Tan, R. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200316409 · Cited by 232
Mucosal AIDS vaccine reduces disease and viral load in gut reservoir and blood after mucosal infection of macaques
Belyakov, I. M., Hel, Z., Kelsall, B. L., Kuznetsov, V. A., Ahlers, J. D., Nacsa, J., Watkins, D. I., Allen, T. M., Sette, A., Altman, J. D., et al. (2001) · Nature Medicine · doi:10.1038/nm1201-1320 · Cited by 232
“Soluble tetrameric Mamu-A a 01/CM9 complex conjugated to PE-labeled streptavidin prepared as describe”
Self-citation
[26] Biotinylation of proteins in vivo and in vitro using small peptide tags
Cull, M. G., & Schatz, P. J. (2000) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(00)26068-0 · Cited by 230
Characterization of the Peptide Binding Motif of a Rhesus MHC Class I Molecule (Mamu-A*01) That Binds an Immunodominant CTL Epitope from Simian Immunodeficiency Virus
Allen, T. M., Sidney, J., del Guercio, M., Glickman, R. L., Lensmeyer, G. L., Wiebe, D. A., DeMars, R., Pauza, C. D., Johnson, R. P., Sette, A., et al. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.160.12.6062 · Cited by 230
“This epitope can now be used to develop epitope-based vaccines, to detect viral escape mutants, or to form soluble peptide-MHC dimers or tetramers (77, 78) for the identification and characterization of peptide-specific CTL.”
The cancer antigenome
Heemskerk, B., Kvistborg, P., & Schumacher, T. N. (2012) · The EMBO Journal · doi:10.1038/emboj.2012.333 · Cited by 239
“The detection of antigen-speciﬁc Tcells independent of their functional capacities ﬁrst became possible when Altman et al (1996) demonstrated that ﬂuorescently labelled multimers of MHC molecules containing a peptide of interest could be used to speciﬁcally detect T cells that recognize this…”
CD8+ T-Cell Responses to Trypanosoma cruzi Are Highly Focused on Strain-Variant trans-Sialidase Epitopes
Martin, D. L., Weatherly, D. B., Laucella, S. A., Cabinian, M. A., Crim, M. T., Sullivan, S., Heiges, M., Craven, S. H., Rosenberg, C. S., Collins, M. H., et al. (2006) · PLoS Pathogens · doi:10.1371/journal.ppat.0020077 · Cited by 228
“focused on a small number of epitopes [2–4], and examination”
Background
The Role of Neoantigens in Naturally Occurring and Therapeutically Induced Immune Responses to Cancer
Ward, J. P., Gubin, M. M., & Schreiber, R. D. (2016) · Advances in immunology · doi:10.1016/bs.ai.2016.01.001 · Cited by 232
“…human tumors identified Coulie et al. (1995) and Wolfel et al. (1995) 1996 Use of peptide–MHC tetramers to analyze antigen-specific T cells Altman et al. (1996) Antitumor activity of anti-CTLA-4 demonstrated in mice Leach, Krummel, and Allison (1996) 1997 Use of SEREX to identify the…”
Background
Bim/Bcl-2 balance is critical for maintaining naive and memory T cell homeostasis
Wojciechowski, S., Tripathi, P., Bourdeau, T., Acero, L. F., Grimes, H. L., Katz, J. D., Finkelman, F. D., & Hildeman, D. A. (2007) · The Journal of Experimental Medicine · doi:10.1084/jem.20070618 · Cited by 227
PD-1-Expressing SARS-CoV-2-Specific CD8+ T Cells Are Not Exhausted, but Functional in Patients with COVID-19
Rha, M., Jeong, H. W., Ko, J. H., Choi, S. J., Seo, I., Lee, J. S., Sa, M., Kim, A. R., Joo, E., Ahn, J. Y., et al. (2020) · Immunity · doi:10.1016/j.immuni.2020.12.002 · Cited by 227
“The limitation of stimulation-based functional assays can be overcome by using major histocompatibility complex class I (MHC class I) multimer techniques (Altman et al., 1996).”
Dressed to kill? A review of why antiviral CD8 T lymphocytes fail to prevent progressive immunodeficiency in HIV-1 infection
Lieberman, J., Shankar, P., Manjunath, N., & Andersson, J. (2001) · Blood · doi:10.1182/blood.v98.6.1667 · Cited by 226
Single-cell mass cytometry for analysis of immune system functional states
Bjornson, Z. B., Nolan, G. P., & Fantl, W. J. (2013) · Current Opinion in Immunology · doi:10.1016/j.coi.2013.07.004 · Cited by 227
“The tetramer can then be detected by flow cytometry via the fluorescent label [82,83].”
In Vivo Killing Capacity of Cytotoxic T Cells Is Limited and Involves Dynamic Interactions and T Cell Cooperativity
Halle, S., Keyser, K. A., Stahl, F. R., Busche, A., Marquardt, A., Zheng, X., Galla, M., Heissmeyer, V., Heller, K., Boelter, J., et al. (2016) · Immunity · doi:10.1016/j.immuni.2016.01.010 · Cited by 231
“After 6–8 days, MCMV-specific CTLs were isolated by tetramer staining (Altman et al., 1996).”
Progression of Armed CTL from Draining Lymph Node to Spleen Shortly After Localized Infection with Herpes Simplex Virus 1
Coles, R. M., Mueller, S. N., Heath, W. R., Carbone, F., & Brööks, A. G. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.2.834 · Cited by 225
CTL Fail to Accumulate at Sites of HIV-1 Replication in Lymphoid Tissue
Connick, E., Mattila, T. L., Folkvord, J. M., Schlichtemeier, R., Meditz, A. L., Ray, M., McCarter, M. D., MaWhinney, S., Hage, A., White, C., et al. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.178.11.6975 · Cited by 224
Selection of T Cell Clones Expressing High-Affinity Public TCRs within Human Cytomegalovirus-Specific CD8 T Cell Responses
Trautmann, L., Rimbert, M., Echasserieau, K., Saulquin, X., Neveu, B., Déchanet‐Merville, J., Cerundolo, V., & Bonneville, M. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.9.6123 · Cited by 224
“Soluble pMHC monomer NLV/A2 was generated as described (24).”
Method
Antigen sensitivity is a major determinant of CD8+ T-cell polyfunctionality and HIV-suppressive activity
Almeida, J. R., Sauce, D., Price, D. A., Papagno, L., Shin, S. Y., Moris, A., Larsen, M., Pancino, G., Douek, D. C., Autran, B., et al. (2009) · Blood · doi:10.1182/blood-2009-02-206557 · Cited by 224
The Role of β7 Integrins in CD8 T Cell Trafficking During an Antiviral Immune Response
Lefrançois, L., Parker, C. M., Olson, S., Müller, W., Wagner, N., & Puddington, L. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.189.10.1631 · Cited by 224
Direct isolation, phenotyping and cloning of low-frequency antigen-specific cytotoxic T lymphocytes from peripheral blood
Dunbar, P. R., Ogg, G. S., Chen, J., Rust, N. A., van der Bruggen, P., & Cerundolo, V. (1998) · Current Biology · doi:10.1016/s0960-9822(98)70161-7 · Cited by 224
“Complexes were biotinylated and bound to phycoerythrin (PE)-labelled streptavidin at a 4:1 ratio, to form an A2–matrix tetramer [1].”
Method
Quantitative and functional analysis of PDC‐E2‐specific autoreactive cytotoxic T lymphocytes in primary biliary cirrhosis
Kita, H., Matsumura, S., He, X.-S., Ansari, A. A., Lian, Z.-X., Van de Water, J., Coppel, R. L., Kaplan, M. M., & Gershwin, M. E. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0214698 · Cited by 222
Specific Cytotoxic T Lymphocyte Responses Against Melan-A/MART1, Tyrosinase and Gp100 in Vitiligo by the Use of Major Histocompatibility Complex/Peptide Tetramers: the Role of Cellular Immunity in the Etiopathogenesis of Vitiligo
Palermo, B., Campanelli, R., Garbelli, S., Mantovani, S., Lantelme, E., Brazzelli, V., Ardigò, M., Borroni, G., Martinetti, M., Badulli, C., et al. (2001) · Journal of Investigative Dermatology · doi:10.1046/j.1523-1747.2001.01408.x · Cited by 222
Differentiation of Human CD8+ T Cells from a Memory to Memory/Effector Phenotype
Tomiyama, H., Matsuda, T., & Takiguchi, M. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.11.5538 · Cited by 220
“HLA class I-peptide tetrameric complexes were synthesized as previously described (30).”
Method
Quantitative and functional analysis of PDC-E2–specific autoreactive cytotoxic T lymphocytes in primary biliary cirrhosis
Kita, H., Matsumura, S., He, X.-S., Ansari, A. A., Lian, Z., de Water, J. V., Coppel, R. L., Kaplan, M. M., & Gershwin, M. E. (2002) · Journal of Clinical Investigation · doi:10.1172/jci14698 · Cited by 222
“PDC-E2159-167-HLA A2 tetramers were generated as described (17).”
A monoclonal cytolytic T-lymphocyte response observed in a melanoma patient vaccinated with a tumor-specific antigenic peptide encoded by gene MAGE-3
Coulie, P. G., Karanikas, V., Colau, D., Lurquin, C., Landry, C., Marchand, M., Dorval, T., Brichard, V. G., & Boon, T. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.161260098 · Cited by 221
Conserved T Cell Receptor Repertoire in Primary and Memory CD8 T Cell Responses to an Acute Viral Infection
Sourdive, D. J. D., Murali‐Krishna, K., Altman, J. D., Zajac, A. J., Whitmire, J. K., Pannetier, C., Kourilsky, P., Evavold, B. D., Sette, A., & Ahmed, R. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.188.1.71 · Cited by 219
“We took advantage of this feature to identify virus-specific T cells by staining with Ld-NP118–126 MHC class I tetramers (25, 46).”
MethodSelf-citation
Differential Antigen Presentation Regulates the Changing Patterns of CD8+ T Cell Immunodominance in Primary and Secondary Influenza Virus Infections
Crowe, S. R., Turner, S. J., Miller, S. C., Roberts, A. D., Rappolo, R. A., Doherty, P. C., Ely, K. H., & Woodland, D. L. (2003) · The Journal of Experimental Medicine · doi:10.1084/jem.20022151 · Cited by 218
“ated by the Molecular Biology Core Facility at the Trudeau Institute as described previously ( 25 ).”
Method
Respiratory syncytial virus infection suppresses lung CD8+ T-cell effector activity and peripheral CD8+ T-cell memory in the respiratory tract
Chang, J., & Braciale, T. J. (2002) · Nature Medicine · doi:10.1038/nm0102-54 · Cited by 218
“MHC class I–peptide tetramers were produced as describe”
Method
EBV-Specific CD8+ T Cell Memory: Relationships Between Epitope Specificity, Cell Phenotype, and Immediate Effector Function
Hislop, A. D., Gudgeon, N. H., Callan, M. F. C., Fazou, C., Hasegawa, H., Salmon, M., & Rickinson, A. B. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.167.4.2019 · Cited by 218
“I/peptide tetramers (15) and on peptide-induced cytokine release”
BackgroundMethod
Detection of T-Cell Degranulation: CD107a and b
Betts, M. R., & Koup, R. A. (2004) · Methods in cell biology · doi:10.1016/s0091-679x(04)75020-7 · Cited by 215
T cell vaccines for microbial infections
Robinson, H. L., & Amara, R. R. (2005) · Nature Medicine · doi:10.1038/nm1212 · Cited by 218
“The tetramer assay directly tests samples for the frequency of CD8 T cells with a specific T cell recepto”
Method
Differentiation of cytomegalovirus-specific CD8+ T cells in healthy and immunosuppressed virus carriers
Gamadia, L. E., Rentenaar, R. J., Baars, P. A., Remmerswaal, E. B. M., Surachno, S., Weel, J. F. L., Toebes, M., Schumacher, T. N., ten Berge, I. J. M., & van Lier, R. A. W. (2001) · Blood · doi:10.1182/blood.v98.3.754 · Cited by 215
Induction of Rapid T Cell Activation and Tolerance by Systemic Presentation of an Orally Administered Antigen
Gütgemann, I., Fahrer, A. M., Altman, J. D., Davis, M. M., & Chien, Y. (1998) · Immunity · doi:10.1016/s1074-7613(00)80571-3 · Cited by 212
Self-citation
Reversible MHC multimer staining for functional isolation of T-cell populations and effective adoptive transfer
Knabel, M., Franz, T. J., Schiemann, M., Wulf, A., Villmow, B., Schmidt, B., Bernhard, H., Wagner, H., & Busch, D. H. (2002) · Nature Medicine · doi:10.1038/nm0602-631 · Cited by 211
“and major histocompatibility complex (MHC) multimer procedure”
Cytomegalovirus reactivation following allogeneic stem cell transplantation is associated with the presence of dysfunctional antigen-specific CD8+ T cells
Özdemir, E., John, L. S. S., Gillespie, G. M. A., Rowland‐Jones, S., Champlin, R. E., Molldrem, J. J., & Komanduri, K. V. (2002) · Blood · doi:10.1182/blood-2002-05-1387 · Cited by 211
Programmed death 1 protects from fatal circulatory failure during systemic virus infection of mice
Frebel, H., Nindl, V., Schuepbach, R. A., Braunschweiler, T., Richter, K., Vogel, J., Wagner, C. A., Loffing‐Cueni, D., Kurrer, M. O., Ludewig, B., et al. (2012) · The Journal of Experimental Medicine · doi:10.1084/jem.20121015 · Cited by 218
“as previously described (Altman et al., 1996).”
Method
Functional leukemia-associated antigen-specific memory CD8+ T cells exist in healthy individuals and in patients with chronic myelogenous leukemia before and after stem cell transplantation
Rezvani, K., Grube, M., Brenchley, J. M., Sconocchia, G., Fujiwara, H., Price, D. A., Emma Gostick, D., YAMADA, K., Melenhorst, J. J., Childs, R. W., et al. (2003) · Blood · doi:10.1182/blood-2003-01-0150 · Cited by 211

Authors: Altman, J. D., Moss, P. A. H., Goulder, P. J. R., Barouch, D. H., McHeyzer-Williams, M. G., Bell, J. I., McMichael, A. J., & Davis, M. M.
Affiliations: Stanford Medicine (US)
John Radcliffe Hospital (GB)
J. D. Altman and M. G. McHeyzer-Williams, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5428, USA.
Howard Hughes Medical Institute (US)
Corresponding author: Davis, Mark M.
ORCID: Altman, John D.
Moss, Paul A. H.
Goulder, Philip J. R.
Barouch, Dan H.
Bell, John I.
McMichael, Andrew J.
Davis, Mark M.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 1996-10-04
Volume: 274
Issue: 5284
Pages: 94-96
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.274.5284.94
OpenAlex: W2067069532
Cited by: 3,544
References: 39
Citations by year: 2026: 44 · 2025: 41 · 2024: 45 · 2023: 59 · 2022: 43 · 2021: 68 · 2020: 59 · 2019: 71 · 2018: 70 · 2017: 42 · 2016: 62 · 2015: 73 · 2014: 69 · 2013: 101 · 2012: 93
FWCI: 35.24
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Funders: Wellcome Trust
Topics: Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
Keywords: antigen-specific T cells, peptide-MHC tetramers, HLA-A2, cytotoxic T lymphocytes, cytotoxicity assay, T cell phenotyping, HIV infection, tumor-specific T cells, autoantigen-specific T cells
MeSH terms: Amino Acid Sequence, Epitopes, Antigens, Viral, Base Sequence, Cell Line, Coloring Agents, Flow Cytometry, HIV Seropositivity, Humans, Molecular Sequence Data, Peptide Fragments, Phenotype, RNA-Directed DNA Polymerase, T-Lymphocytes, Cytotoxic, Viral Matrix Proteins, Gene Products, gag, HLA-A2 Antigen, CD8-Positive T-Lymphocytes

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Phenotypic Analysis of Antigen-Specific T Lymphocytes

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