Cited by 3,544T-cell and B-cell ImmunologyImmunotherapy and Immune Responsesvaccines and immunoinformatics approaches

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Phenotypic Analysis of Antigen-Specific T Lymphocytes

Altman, John D., Moss, Paul A. H., Goulder, Philip J. R., Barouch, Dan H., McHeyzer-Williams, Michael G., Bell, John I., McMichael, Andrew J., Davis, Mark M.·1996·Science

DOI 10.1126/science.274.5284.94

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Abstract

Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays. This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens.

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“A newly developed approach based on T-cell receptor interaction with antigenic complexes in the form of tetramers (41) may allow detection of low numbers of (viral) peptide-specific CTLs.”
Method
Interaction between the CD8 Coreceptor and Major Histocompatibility Complex Class I Stabilizes T Cell Receptor-Antigen Complexes at the Cell Surface
Wooldridge, L., van den Berg, H. A., Glick, M., Emma Gostick, D., Laugel, B., Hutchinson, S., Milicic, A., Brenchley, J. M., Douek, D. C., Price, D. A., et al. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m500555200 · Cited by 168
Identification of NY-ESO-1 Peptide Analogues Capable of Improved Stimulation of Tumor-Reactive CTL
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“HLA class I-peptide tetrameric complexes (tetramers) were synthesized, as previously described (18).”
Method
Peptide–MHC-based nanomedicines for autoimmunity function as T-cell receptor microclustering devices
Singha, S., Shao, K., Yang, Y., Clemente‐Casares, X., Solé, P., Clemente, A., Blanco, J., Dai, Q., Song, F., Liu, S. W., et al. (2017) · Nature Nanotechnology · doi:10.1038/nnano.2017.56 · Cited by 166
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“The tetramers were prepared by us as described previously (1).”
Method
Transregulation of memory CD8 T-cell proliferation by IL-15Rα+ bone marrow–derived cells
Schluns, K. S., Klonowski, K. D., & Lefrançois, L. (2003) · Blood · doi:10.1182/blood-2003-08-2814 · Cited by 165
Transfer of Specificity for Human Immunodeficiency Virus Type 1 into Primary Human T Lymphocytes by Introduction of T-Cell Receptor Genes
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“It is often difficult to isolate CTL from individuals with advanced disease with expended CTL responses (10), as well as individuals receiving prolonged therapy with highly active antiretroviral therapy, due to the fall in precursor frequency of CTL to HIV following the reduction in viral burden (2).”
Background
A PR1-human leukocyte antigen-A2 tetramer can be used to isolate low-frequency cytotoxic T lymphocytes from healthy donors that selectively lyse chronic myelogenous leukemia.
Molldrem, J. J., Lee, P. P., Wang, C., Champlin, R. E., & Davis, M. M. (1999) · PubMed · Cited by 165
Self-citation
Strategies for immunotherapy of cancer
Melief, C. J. M., Toes, R. E. M., Medema, J. P., van der Burg, S. H., OSSENDORP, F. A., & Offringa, R. (2000) · Advances in immunology · doi:10.1016/s0065-2776(00)75006-1 · Cited by 167
Increased CD95/Fas-Induced Apoptosis of HIV-Specific CD8+ T Cells
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Self-citation
CD8 + T cells specific for conserved coronavirus epitopes correlate with milder disease in patients with COVID-19
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“antibodies to stain epitope-specific CD8 T cells as described previously (4, 19).”
BackgroundSelf-citation
B‐cell responses to HIV infection
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Self-citation
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“Taking advantage of constructs previously designed for other CD1 proteins, we produced transmembrane-truncated, biotinylated CD1a proteins zippered to β -2 microglobulin ( β 2 M) with high yields in HEK293 cells 15,16 (Fig.”
BackgroundSelf-citation
IL-12 and type I interferon prolong the division of activated CD8 T cells by maintaining high-affinity IL-2 signaling in vivo
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“MHC class I tetramers (Kb) specific for OVA257–264 were prepared using published protocols (Altman et al., 1996; Busch et al., 1998).”
Method
Viral and Bacterial Infections Induce Expression of Multiple NK Cell Receptors in Responding CD8+ T Cells
McMahon, C. W., Zajac, A. J., Jamieson, A. M., Corral, L. G., Hammer, G. E., Ahmed, R., & Raulet, D. H. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.169.3.1444 · Cited by 162
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“More recently developed immunologic methods, including major histocompatibility complex (MHC)-tetramers, intracellular cytokine detection and ELISPOT assays, have greatly improved sensitivity to enumerate antigen-specific T cells; however, these newer methods do not assess the cytolytic function of antigen-specific CT…”
BackgroundSelf-citation
Flow-cytometric analysis of rare antigen-specific T cells
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“of peptide-MHC complexes to increase the relative binding avidity (22).”
Background
Circulating CD8 T Lymphocytes in Human Immunodeficiency Virus-Infected Individuals Have Impaired Function and Downmodulate CD3ζ, the Signaling Chain of the T-Cell Receptor Complex
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Expression of Costimulatory Ligand CD70 on Steady-State Dendritic Cells Breaks CD8+ T Cell Tolerance and Permits Effective Immunity
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“Flow Cytometry Tetrameric complexes of biotinylated murine MHC class I H-2D or H-2K heavy chain, b2 microglobulin, and relevant peptide were prepared as described (Altman et al., 1996), labeled with PE- or APC-conjugated streptavidin, and used in combination with CD8a mAb 53-6.”
MethodInfluential
Novel application of a whole blood intracellular cytokine detection assay to quantitate specific T-cell frequency in field studies
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MethodSelf-citation
CD8+ T-cell selection, function, and death in the primary immune response in vivo
Callan, M. F. C., Fazou, C., Yang, H., Rostron, T., Poon, K., Hatton, C., & McMichael, A. J. (2000) · Journal of Clinical Investigation · doi:10.1172/jci10590 · Cited by 158
“Soluble HLA/peptide tetrameric complexes were produced as described previously (4, 27).”
MethodSelf-citation
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Self-citation
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“HLA class I tetramers have been used to identify HLA-specific primary T cells in peripheral blood but the affinity of the KIR/HLA interaction is low and binding of HLA-C tetramers to primary NK cells has not been described (23).”
Method
Interplay of Cellular and Humoral Immune Responses against BK Virus in Kidney Transplant Recipients with Polyomavirus Nephropathy
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“plasmid HLA-A*0201/glyser/BirA substrate peptide (1) and refolded in vitro”
Bystander T cells in cancer immunology and therapy
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Tetramer visualization of gut-homing gluten-specific T cells in the peripheral blood of celiac disease patients
Ráki, M., Fallang, L.-E., Brottveit, M., Bergseng, E., Quarsten, H., Lundin, K. E. A., & Sollid, L. M. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0608610104 · Cited by 156
Protein kinase inhibitors substantially improve the physical detection of T-cells with peptide-MHC tetramers
Lissina, A., Ladell, K., Skowera, A., Clement, M., Edwards, E. S. J., Seggewiss, R., van den Berg, H. A., Emma Gostick, D., Gallagher, K., Jones, E., et al. (2008) · Journal of Immunological Methods · doi:10.1016/j.jim.2008.09.014 · Cited by 154
“their visualization, enumeration, phenotypic characterization and isolation from ex vivo samples (Altman et al., 1996; Chattopadhyay et al., 2006).”
BackgroundInfluential
Longitudinal Phenotypic Analysis of Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Lymphocytes: Correlation with Disease Progression
Ogg, G. S., Kostense, S., Klein, M. R., Jurriaans, S., Hamann, D., McMichael, A. J., & Miedema, F. F. (1999) · Journal of Virology · doi:10.1128/jvi.73.11.9153-9160.1999 · Cited by 156
Self-citation
The Role of Antigen in the Localization of Naive, Acutely Activated, and Memory CD8+ T Cells to the Lung During Influenza Pneumonia
Topham, D. J., Castrucci, M. R., Wingo, F. S., Belz, G. T., & Doherty, P. C. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.167.12.6983 · Cited by 155
Spontaneous Regression of Primary Autoreactivity during Chronic Progression of Experimental Autoimmune Encephalomyelitis and Multiple Sclerosis
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“The actual level of self-recognition plasticity may best be estimated by serial assessment of the autoreactive changes to overlapping peptides of several myelin proteins implicated in MS autoreactivity, such as PLP, MBP, and MOG, as well as by the incorporation of more sensitive assays such as the ELISPOT capable of p…”
Background
Antigen-specific activation and cytokine-facilitated expansion of naive, human CD8+ T cells
Wölfl, M., & Greenberg, P. D. (2014) · Nature Protocols · doi:10.1038/nprot.2014.064 · Cited by 154
Identification of Five MAGE-A1 Epitopes Recognized by Cytolytic T Lymphocytes Obtained by In Vitro Stimulation with Dendritic Cells Transduced with MAGE-A1
Chaux, P., Luiten, R. M., Demotte, N., Vantomme, V., Stroobant, V., Traversari, C., Russo, V. E. A., Schultz, E. S., Cornelis, G. R., Boon, T., et al. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.5.2928 · Cited by 156
“One promising possibility, which nevertheless restricts the analysis to certain epitopes, is the use of a set of relevant peptides in combination with soluble HLA tetramers to label TCRs directly (50, 51).”
Background
Adoptive tumor therapy with T lymphocytes enriched through an IFN-γ capture assay
Becker, C., Pohla, H., Frankenberger, B., Schüler, T., Assenmacher, M., Schendel, D. J., & Blankenstein, T. J. F. (2001) · Nature Medicine · doi:10.1038/nm1001-1159 · Cited by 154
Competition Between CTL Narrows the Immune Response Induced by Prime-Boost Vaccination Protocols
Palmowski, M. J., Choi, E. M.-L., Hermans, I. F., Gilbert, S. C., Chen, J., Gileadi, U., Salio, M., Pel, A. V., Man, S., Bonin, E., et al. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.9.4391 · Cited by 154
“Tetrameric A2/peptide complexes were synthesized as previously described (1).”
Method
Environmental cues dictate the fate of individual CD8+ T cells responding to infection
Plumlee, C. R., Sheridan, B., Cicek, B. B., & Lefrançois, L. (2013) · Immunity · doi:10.1016/j.immuni.2013.07.014 · Cited by 160
“Flow Cytometry OVA- and N-specific T cells were stained with the H-2Kb tetramer containing the OVA peptide (SIINFEKL) and the H-2Kb tetramer containing the N peptide (RGYVYQGL) generated in our laboratory as previously described (Altman et al., 1996; Masopust et al., 2001).”
Developmental Regulation of Lck Targeting to the CD8 Coreceptor Controls Signaling in Naive and Memory T Cells
Bachmann, M. F., Gallimore, A., Linkert, S., Cerundolo, V., Lanzavecchia, A., Köpf, M., & Viola, A. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.189.10.1521 · Cited by 153
“Soluble, biotinylated class I monomers, comprising the murine D b molecule, human b 2-microglobulin, and lymphocytic choriomeningitis virus (LCMV) 1 peptide p33, were generated as described previously (24, 25).”
Method
T cell antigen discovery
Joglekar, A. V., & Li, G. (2020) · Nature Methods · doi:10.1038/s41592-020-0867-z · Cited by 152
Efficient detection and immunomagnetic sorting of specific T cells using multimers of MHC class I and peptide with reduced CD8 binding
Bodinier, M., Peyrat, M., Tournay, C., Davodeau, F., Romagné, F., Bonneville, M., & Lang, F. (2000) · Nature Medicine · doi:10.1038/76292 · Cited by 152
Human T lymphocytes transduced by lentiviral vectors in the absence of TCR activation maintain an intact immune competence
Cavalieri, S., Cazzaniga, S., Geuna, M., Magnani, Z. I., Bordignon, C., Naldini, L., & Bonini, C. (2003) · Blood · doi:10.1182/blood-2003-01-0297 · Cited by 152
Risks and benefits of structured antiretroviral drug therapy interruptions in HIV-1 infection
Bonhoeffer, S., Rembiszewski, M., Ortiz, G. M., & Nixon, D. F. (2000) · AIDS · doi:10.1097/00002030-200010200-00012 · Cited by 155
“Consequently, for the evaluation of the clinical data from STI trials it is crucial to obtain accurate quantitative measurements of the effector cell populations (both armed and memory effector cells) before, during, and after therapy, using assays such as the limiting dilution and/or chromium release assays [12,26] t…”
Method
Thymic Selection Generates a Large T Cell Pool Recognizing a Self-Peptide in Humans
Zippelius, A., Pittet, M. J., Batard, P., Rufer, N., Smedt, M. D., Guillaume, P., Ellefsen, K., Valmori, D., Líénard, D., Plum, J. R., et al. (2002) · The Journal of Experimental Medicine · doi:10.1084/jem.20011658 · Cited by 156
Conditional MHC class I ligands and peptide exchange technology for the human MHC gene products HLA-A1, -A3, -A11, and -B7
Bakker, A., Hoppes, R., Linnemann, C., Toebes, M., Rodenko, B., Berkers, C. R., Hadrup, S. R., van Esch, W. J. E., Heemskerk, M. H. M., Ovaa, H., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0709717105 · Cited by 151
Increasing functional avidity of TCR-redirected T cells by removing defined N -glycosylation sites in the TCR constant domain
Kuball, J. H. E., Hauptrock, B., Malina, V., Antunes, E., Voss, R.-H., Wölfl, M., Strong, R. K., Theobald, M., & Greenberg, P. D. (2009) · The Journal of Experimental Medicine · doi:10.1084/jem.20082487 · Cited by 151
The role of CD4+ T helper cells in the cytotoxic T lymphocyte response to HIV-1
Altfeld, M. A., & Rosenberg, E. S. (2000) · Current Opinion in Immunology · doi:10.1016/s0952-7915(00)00103-5 · Cited by 151
IL-21–mediated Foxp3 suppression leads to enhanced generation of antigen-specific CD8+ cytotoxic T lymphocytes
Li, Y., & Yee, C. (2007) · Blood · doi:10.1182/blood-2007-05-089375 · Cited by 150
Dynamics of T Cell Responses in HIV Infection
Appay, V., Papagno, L., Spina, C. A., Hansasuta, P., King, A. S., Jones, L. E., Ogg, G. S., Little, S. J., McMichael, A. J., Richman, D. D., et al. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.7.3660 · Cited by 152
“The HLA molecule H chain cDNAs were modified by substitution of the transmembrane and cytosolic regions with a sequence encoding the BirA biotinylation enzyme recognition site, as previously described (18).”
Self-citation
Immunity to Listeria infection
Edelson, B. T., & Unanue, E. R. (2000) · Current Opinion in Immunology · doi:10.1016/s0952-7915(00)00112-6 · Cited by 151
Identification of Major Epitopes of Mycobacterium tuberculosis AG85B That Are Recognized by HLA-A*0201-Restricted CD8+ T Cells in HLA-Transgenic Mice and Humans
Geluk, A., van Meijgaarden, K. E., Franken, K. L. M. C., Drijfhout, J. W., D’Souza, S., Necker, A., Huygen, K., & Ottenhoff, T. H. M. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.11.6463 · Cited by 151
“MHC/peptide tetramers were produced as described previously (32), but to abrogate CD8 binding, a mutated HLA-A*0201 heavy chain (denoted A2 m) was used, containing the mutation A2453V.”
Method
CD160 and PD-1 Co-Expression on HIV-Specific CD8 T Cells Defines a Subset with Advanced Dysfunction
Peretz, Y., He, Z., Shi, Y., Yassine‐Diab, B., Goulet, J.-P., Bordi, R., Filali‐Mouhim, A., Loubert, J.-B., Far, M. E., Dupuy, F. P., et al. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1002840 · Cited by 150
“Plasma viral loads were measured with the Amplicor HIV-1 Monitor Ultrasensitive method with a limit of detection of 50 HIV-1 RNA copies/mL of plasma (Roche Diagnostics, Mississauga, Canada).”
MethodInfluential
A MAGE-A3 peptide presented by HLA-DP4 is recognized on tumor cells by CD4+ cytolytic T lymphocytes.
Schultz, E. S., Lethé, B., Cambiaso, C. L., Snick, J. V., Chaux, P., Corthals, J., Heirman, C., Thielemans, K., Boon, T., & van der Bruggen, P. (2000) · PubMed · Cited by 150
Putative Immunodominant Human Immunodeficiency Virus-Specific CD8 + T-Cell Responses Cannot Be Predicted by Major Histocompatibility Complex Class I Haplotype
Betts, M. R., Casazza, J. P., Patterson, B. A., Waldrop, S. L., Trigona, W. L., Fu, T., Kern, F., Picker, L. J., & Koup, R. A. (2000) · Journal of Virology · doi:10.1128/jvi.74.19.9144-9151.2000 · Cited by 150
“With the development of major histocompatibility complex (MHC) class I tetramer technology (1), studies have quantified the CD8(1) T-cell populations specific for individual HIV peptides and correlated these findings with various HIV disease parameters (21, 22).”
Background
New Generation Vaccine Induces Effective Melanoma-Specific CD8+ T Cells in the Circulation but Not in the Tumor Site
Appay, V., Jandus, C., Voelter, V., Reynard, S., Coupland, S. E., Rimoldi, D., Líénard, D., Guillaume, P., Krieg, A. Μ., Cerottini, J., et al. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.177.3.1670 · Cited by 152
Tumor Rejection Induced by CD70-mediated Quantitative and Qualitative Effects on Effector CD8+ T Cell Formation
Arens, R., Schepers, K., Nolte, M. A., van Oosterwijk, M. F., van Lier, R. A. W., Schumacher, T. N., & van Oers, M. H. J. (2004) · The Journal of Experimental Medicine · doi:10.1084/jem.20031111 · Cited by 149
Correlation between tumor regression and T cell responses in melanoma patients vaccinated with a MAGE antigen
Lonchay, C., van der Bruggen, P., Connerotte, T., Hanagiri, T., Coulie, P. G., Colau, D., Lucas, S., Pel, A. V., Thielemans, K., van Baren, N., et al. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0405743101 · Cited by 149
The Human CD8 Coreceptor Effects Cytotoxic T Cell Activation and Antigen Sensitivity Primarily by Mediating Complete Phosphorylation of the T Cell Receptor ζ Chain
Purbhoo, M. A., Boulter, J. M., Price, D. A., Vuidepot, A., Hourigan, C. S., Dunbar, P. R., Olson, K., Dawson, S. J., Phillips, R. E., Jakobsen, B. K., et al. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m102498200 · Cited by 149
Self-citation
Discovery of rare cells from voluminous single cell expression data
Jindal, A., Gupta, P., Jayadeva, & Sengupta, D. (2018) · Nature Communications · doi:10.1038/s41467-018-07234-6 · Cited by 148
Direct Analysis of the Dynamics of the Intestinal Mucosa CD8 T Cell Response to Systemic Virus Infection
Masopust, D., Jiang, J. K., Shen, H., & Lefrançois, L. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.4.2348 · Cited by 148
“MHC tetramers were produced essentially as previously described (14, 31).”
Method
Positive selection of MHC class Ib–restricted CD8+ T cells on hematopoietic cells
Urdahl, K. B., Sun, J. C.-Y., & Bevan, M. J. (2002) · Nature Immunology · doi:10.1038/ni814 · Cited by 148
“H-2K b tetramers bound to the OVA-derived peptide SIINFEKL were generated as describe”
Method
Oligoclonal Expansions of CD8+ T Cells in Chronic HIV Infection Are Antigen Specific
Wilson, J., Ogg, G. S., Allen, R. L., Goulder, P., Kelleher, A. D., Sewell, A. K., O’Callaghan, C. A., Rowland‐Jones, S., Callan, M. F. C., & McMichael, A. J. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.188.4.785 · Cited by 148
“Tetrameric MHC–peptide complexes, which bind specifically to appropriate MHC–peptide-specific T cells (14) has been used to show that the large CD8 1 T cell expansions during the acute phase of EBV and lymphocytic choriomeningitis virus are largely antigen specific (15, 16).”
MethodSelf-citation
Proliferation Requirements of Cytomegalovirus-Specific, Effector-Type Human CD8+ T Cells
van Leeuwen, E. M. M., Gamadia, L. E., Baars, P. A., Remmerswaal, E. B. M., ten Berge, I. J. M., & van Lier, R. A. W. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.169.10.5838 · Cited by 147
CD8+Lymphocytes from Simian Immunodeficiency Virus-Infected Rhesus Macaques Recognize 14 Different Epitopes Bound by the Major Histocompatibility Complex Class I Molecule Mamu-A*01: Implications for Vaccine Design and Testing
Allen, T. M., Mothé, B. R., Sidney, J., Jing, P., Dzuris, J. L., Liebl, M. E., Vogel, T. U., O’Connor, D. H., Wang, X., Wussow, M. C., et al. (2001) · Journal of Virology · doi:10.1128/jvi.75.2.738-749.2001 · Cited by 147
“Gag_CM9 peptide complexes were constructed as previously described (3, 5).”
MethodSelf-citation
Characterization of two TCR transgenic mouse lines specific for herpes simplex virus
Mueller, S. N., Heath, W. R., McLain, J. D., Carbone, F., & Jones, C. M. (2002) · Immunology and Cell Biology · doi:10.1046/j.1440-1711.2002.01071.x · Cited by 147
4-1BB and OX40 Act Independently to Facilitate Robust CD8 and CD4 Recall Responses
Dawicki, W., Bertram, E. M., Sharpe, A. H., & Watts, T. H. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.10.5944 · Cited by 147
Broad, Intense Anti-Human Immunodeficiency Virus (HIV) Ex Vivo CD8 + Responses in HIV Type 1-Infected Patients: Comparison with Anti-Epstein-Barr Virus Responses and Changes during Antiretroviral Therapy
Dalod, M., Dupuis, M., Deschemin, J., Sicard, D., Salmon, D., Delfraissy, J., Venet, A., Sinet, M., & Guillet, J. (1999) · Journal of Virology · doi:10.1128/jvi.73.9.7108-7116.1999 · Cited by 147
“Total anti-HIV (X) and anti-EBV (1) responses calculated as the sum of the SFC numbers for all peptides recognized are also indicated.”
BackgroundResult
Epstein–Barr virus-specific CD8+ T cells that re-express CD45RA are apoptosis-resistant memory cells that retain replicative potential
Dunne, P. J., Faint, J. M., Gudgeon, N. H., Fletcher, J. M., Plunkett, F. J., Soares, M. V. D., Hislop, A. D., Annels, N. E., Rickinson, A. B., Salmon, M., et al. (2002) · Blood · doi:10.1182/blood-2002-01-0160 · Cited by 144
Antigen-Specific CD8+ T Cells Persist in the Upper Respiratory Tract Following Influenza Virus Infection
Wiley, J. A., Hogan, R. J., Woodland, D. L., & Harmsen, A. G. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.167.6.3293 · Cited by 146
“These tetrameric complexes were synthesized by the Molecular Core Unit at the Trudeau Institute according to previously described procedures (14).”
Method
The Liver: A Special Case in Transplantation Tolerance
Benseler, V., McCaughan, G. W., Schlitt, H. J., Bishop, G. A., Bowen, D. G., & Bertolino, P. (2007) · Seminars in Liver Disease · doi:10.1055/s-2007-979471 · Cited by 146
Kupffer Cell-Dependent Hepatitis Occurs during Influenza Infection
Polakos, N. K., Cornejo, J. C., Murray, D. A., Wright, K. O., Treanor, J. J., Crispe, I. N., Topham, D. J., & Pierce, R. H. (2006) · American Journal Of Pathology · doi:10.2353/ajpath.2006.050875 · Cited by 146
CpG Are Efficient Adjuvants for Specific CTL Induction Against Tumor Antigen-Derived Peptide
Miconnet, I., Koenig, S., Speiser, D. E., Krieg, A. Μ., Guillaume, P., Cerottini, J., & Romero, P. J. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.3.1212 · Cited by 146
“5–1 10(6)) were prepared from peripheral blood, inguinal and paraaortic lymph nodes, and spleen from immunized mice and were stained with PE-coupled HLA-A2/ELA26–35 (ELAGIGILTV) tetramer synthesized as previously described (26) at doses indicated in the text in the presence of anti-FcR Ab (clone 2.”
Method
Engagement of CD160 receptor by HLA-C is a triggering mechanism used by circulating natural killer (NK) cells to mediate cytotoxicity
Bouteiller, P. P. L., Barakonyi, A., Giustiniani, J., Lenfant, F., Marie‐Cardine, A., Aguerre‐Girr, M., Rabot, M., Hilgert, I., Mami‐Chouaib, F., Tabiasco, J., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.012681099 · Cited by 146
Evolution of the T Cell Repertoire During Primary, Memory, and Recall Responses to Viral Infection
Blattman, J. N., Sourdive, D. J. D., Murali‐Krishna, K., Ahmed, R., & Altman, J. D. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.11.6081 · Cited by 146
“MHC I tetramers were prepared and refolded with the appropriate peptide and b2-microglobulin as previously described (35).”
MethodSelf-citation
Cell-surface exposure of phosphatidylserine correlates with the stage of fludarabine-induced apoptosis in chronic lymphocytic leukemia and expression of apoptosis-regulating genes
Clodi, K., Kliche, K.-O., Zhao, S., Weidner, D. A., Schenk, T., Consoli, U., Jiang, S., Snell, V., & Andreeff, M. (2000) · Cytometry · doi:10.1002/(sici)1097-0320(20000501)40:1 · Cited by 50
Human cytomegalovirus tropism for endothelial/epithelial cells: scientific background and clinical implications
Revello, M. G., & Gerna, G. (2010) · Reviews in Medical Virology · doi:10.1002/rmv.645 · Cited by 145
Melanoma-specific tumor-infiltrating lymphocytes but not circulating melanoma-specific T cells may predict survival in resected advanced-stage melanoma patients
Haanen, J. B. A. G., Baars, A., Gomez, R., Weder, P., Smits, M., de Gruijl, T. D., Blomberg, B. M. E. V., Bloemena, E., Scheper, R. J., van Ham, S. M., et al. (2005) · Cancer Immunology Immunotherapy · doi:10.1007/s00262-005-0018-5 · Cited by 152
“HLA class I tetrameric complexes were prepared as previously described with some modifications [17].”
Method
Functional Subsets of Memory T Cells Identified by CCR7 Expression
Sallusto, F., Langenkamp, A., Geginat, J., & Lanzavecchia, A. (2000) · Current topics in microbiology and immunology · doi:10.1007/978-3-642-57276-0_21 · Cited by 145
Induction and Visualization of Mucosal Memory CD8 T Cells Following Systemic Virus Infection
Kim, S.-K., Schluns, K. S., & Lefrançois, L. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.8.4125 · Cited by 145
Immunologic Control of HIV-1
Gandhi, R. T., & Walker, B. D. (2002) · Annual Review of Medicine · doi:10.1146/annurev.med.53.082901.104011 · Cited by 143
In vivo proliferation of naïve and memory influenza-specific CD8 + T cells
Flynn, K. J., Riberdy, J. M., Christensen, J. P., Altman, J. D., & Doherty, P. C. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.15.8597 · Cited by 145
Self-citation
The CIMT-monitoring panel: a two-step approach to harmonize the enumeration of antigen-specific CD8+ T lymphocytes by structural and functional assays
Britten, C. M., Gouttefangeas, C., Welters, M. J. P., Pawelec, G., Koch, S. D., Ottensmeier, C. H. H., Mander, A., Walter, S., Paschen, A., Müller‐Berghaus, J., et al. (2007) · Cancer Immunology Immunotherapy · doi:10.1007/s00262-007-0378-0 · Cited by 143
“The enzyme-linked immunospot (ELI-SPOT) assay [10, 11], staining with HLA-peptide multimers [ 12 ] and intracellular cytokine staining (ICS) [13, 14] are technologies used commonly for the monitoring of antigen-speciWc immune responses.”
Method

Authors: Altman, J. D., Moss, P. A. H., Goulder, P. J. R., Barouch, D. H., McHeyzer-Williams, M. G., Bell, J. I., McMichael, A. J., & Davis, M. M.
Affiliations: Stanford Medicine (US)
John Radcliffe Hospital (GB)
J. D. Altman and M. G. McHeyzer-Williams, Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305-5428, USA.
Howard Hughes Medical Institute (US)
Corresponding author: Davis, Mark M.
ORCID: Altman, John D.
Moss, Paul A. H.
Goulder, Philip J. R.
Barouch, Dan H.
Bell, John I.
McMichael, Andrew J.
Davis, Mark M.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 1996-10-04
Volume: 274
Issue: 5284
Pages: 94-96
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.274.5284.94
OpenAlex: W2067069532
Cited by: 3,544
References: 39
Citations by year: 2026: 44 · 2025: 41 · 2024: 45 · 2023: 59 · 2022: 43 · 2021: 68 · 2020: 59 · 2019: 71 · 2018: 70 · 2017: 42 · 2016: 62 · 2015: 73 · 2014: 69 · 2013: 101 · 2012: 93
FWCI: 35.24
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Funders: Wellcome Trust
Topics: Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
Keywords: antigen-specific T cells, peptide-MHC tetramers, HLA-A2, cytotoxic T lymphocytes, cytotoxicity assay, T cell phenotyping, HIV infection, tumor-specific T cells, autoantigen-specific T cells
MeSH terms: Amino Acid Sequence, Epitopes, Antigens, Viral, Base Sequence, Cell Line, Coloring Agents, Flow Cytometry, HIV Seropositivity, Humans, Molecular Sequence Data, Peptide Fragments, Phenotype, RNA-Directed DNA Polymerase, T-Lymphocytes, Cytotoxic, Viral Matrix Proteins, Gene Products, gag, HLA-A2 Antigen, CD8-Positive T-Lymphocytes

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Phenotypic Analysis of Antigen-Specific T Lymphocytes

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