Cited by 802Estrogen and related hormone effectsGenomics and Chromatin DynamicsDiffusion and Search Dynamics

The glucocorticoid receptor: rapid exchange with regulatory sites in living cells

McNally, James G., Müller, Waltraud G., Walker, Dawn, Wolford, Ronald, Hager, Gordon L.·2000·Science

Abstract

Steroid receptors bind to site-specific response elements in chromatin and modulate gene expression in a hormone-dependent fashion. With the use of a tandem array of mouse mammary tumor virus reporter elements and a form of glucocorticoid receptor labeled with green fluorescent protein, targeting of the receptor to response elements in live mouse cells was observed. Photobleaching experiments provide direct evidence that the hormone-occupied receptor undergoes rapid exchange between chromatin and the nucleoplasmic compartment. Thus, the interaction of regulatory proteins with target sites in chromatin is a more dynamic process than previously believed.

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Crossref · 10.1126/science.287.5456.1262

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32 of 32 references verified0 citation errors · 0 not checkable

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  • Once in the nucleus, liganded glucocorticoid receptors do not bind DNA stably but constantly shuttle between the nucleoplasm and glucocorticoid-responsive elements.

    “Once in the nucleus, liganded GRs do not bind to DNA in a stable manner. They rather constantly shuttle between the nucleoplasm and GC-responsive elements (GRE) located in the promoter or enhancer regions of GC-responsive genes (80, 81).”

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Authors
McNally, J. G., Müller, W. G., Walker, D., Wolford, R., & Hager, G. L.
Affiliations
  • National Institutes of Health (US)
  • Laboratory of Receptor Biology and Gene Expression, Building 41, Room B602, National Cancer Institute, Bethesda, MD 20892–5055, USA.
Corresponding author
  • McNally, James G.
  • Hager, Gordon L.
Venue
Science
Published
2000-02-18
Volume
287
Issue
5456
Pages
1262-1265
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2096594220
Cited by
802
References
32
Citations by year
2026: 17 · 2025: 13 · 2024: 11 · 2023: 13 · 2022: 16 · 2021: 21 · 2020: 17 · 2019: 13 · 2018: 15 · 2017: 24 · 2016: 28 · 2015: 27 · 2014: 29 · 2013: 31 · 2012: 20
FWCI
35.70
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Diffusion and Search Dynamics
Keywords
glucocorticoid receptor, chromatin, response elements, nucleoplasm, photobleaching, green fluorescent protein tagging, chromatin dynamics, transcriptional regulation
MeSH terms
Animals, Binding Sites, Cell Line, Transformed, Cell Nucleus, Chromatin, Dexamethasone, Ligands, Luminescent Proteins, Mammary Tumor Virus, Mouse, Microscopy, Fluorescence, Nucleosomes, Receptors, Glucocorticoid, In Situ Hybridization, Fluorescence, Microscopy, Confocal, Terminal Repeat Sequences, Response Elements, Green Fluorescent Proteins, Mice

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