Cited by 485Autophagy in Disease and TherapyCellular transport and secretionUbiquitin and proteasome pathways

The ATG conjugation systems are important for degradation of the inner autophagosomal membrane

Tsuboyama, Kotaro, Koyama-Honda, Ikuko, Sakamaki, Yuriko, Koike, Masato, Morishita, Hideaki, Mizushima, Noboru·2016·Science

Abstract

Open sesame!: The autophagosome is a double-membraned intracellular structure involved in the disposal of damaged or defunct organelles. Autophagosome formation requires a number of autophagy-related (ATG) proteins. Among them, the key conjugation systems ATG8 and ATG12 are widely exploited in the detection of autophagy in many organisms. However, their precise function in autophagy remains unknown. Tsuboyama et al. identified an unexpected role of ATG3, an important enzyme in the ATG conjugation systems, in efficient degradation and opening of the inner autophagosomal membrane after fusion with lysosomes (see the Perspective by Levine). Their live-imaging system revealed the entire life of an autophagosome in mammalian cells. Science , this issue p. 1036 ; see also p. 968

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Crossref · 10.1126/science.aaf6136

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Authors
Tsuboyama, K., Koyama-Honda, I., Sakamaki, Y., Koike, M., Morishita, H., & Mizushima, N.
Affiliations
  • The University of Tokyo (JP)
  • Department of Biochemistry and Molecular Biology, Graduate School and Faculty of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
  • Tokyo Medical and Dental University (JP)
  • Juntendo University (JP)
Corresponding author
  • Tsuboyama, Kotaro
  • Mizushima, Noboru
Venue
Science
Published
2016-11-25
Volume
354
Issue
6315
Pages
1036-1041
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2533025094
Cited by
485
References
36
Citations by year
2026: 25 · 2025: 30 · 2024: 55 · 2023: 39 · 2022: 50 · 2021: 64 · 2020: 69 · 2019: 57 · 2018: 47 · 2017: 43 · 2016: 6
FWCI
47.84
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
Japan Society for the Promotion of Science
Topics
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
Keywords
macroautophagy, autophagosome formation, STX17, autophagosome-lysosome fusion, autophagy-related proteins
MeSH terms
Autophagosomes, Autophagy-Related Protein 8 Family, Autophagy-Related Protein 12, Autophagy, HeLa Cells, Humans, Intracellular Membranes, Lysosomes, Membrane Fusion, Phosphatidylethanolamines, Qa-SNARE Proteins, HEK293 Cells

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