Cited by 102CRISPR and Genetic EngineeringGene Regulatory Network AnalysisAdvanced biosensing and bioanalysis techniques

Emerging applications for DNA writers and molecular recorders

Open access
Farzadfard, Fahim, Lu, Timothy K.·2018·Science

Abstract

Natural life is encoded by evolvable, DNA-based memory. Recent advances in dynamic genome-engineering technologies, which we collectively refer to as in vivo DNA writing, have opened new avenues for investigating and engineering biology. This Review surveys these technological advances, outlines their prospects and emerging applications, and discusses the features and current limitations of these technologies for building various genetic circuits for processing and recording information in living cells.

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Crossref · 10.1126/science.aat9249

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  • Amplified material was pure and ready for re-encapsulation into silica particles, which could be re-introduced directly back into the data pool and thereby represents a complete write-store-access-read cycle that in principle may be applied to larger-scale datasets, with periodic renewal of single-stranded DNA barcodes and bacterial replication of DNA data following reading.

    “Amplified material was pure and ready for re-encapsulation into silica particles, which could be re-introduced directly back into the data pool and thereby represents a complete write-store-access-read cycle that in principle may be applied larger-scale datasets, with periodic renewal of single-stranded DNA barcodes and bacterial replication of DNA data following reading25–27.”

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Research works printed but not linked: 2

  1. [6]Farzadfard F., bioRxiv 263657 [Preprint]. 16 2 2018.
  2. [16]Meyer AJ, Segall-Shapiro TH, Voigt CA, bioRxiv 285866 [Preprint]. 10 4 2018.

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Authors
Farzadfard, F., & Lu, T. K.
Affiliations
Massachusetts Institute of Technology (US)
Venue
Science
Published
2018-08-31
Volume
361
Issue
6405
Pages
870-875
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2888880210
Cited by
102
References
28
Citations by year
2026: 7 · 2025: 11 · 2024: 8 · 2023: 10 · 2022: 11 · 2021: 20 · 2020: 15 · 2019: 19 · 2018: 1
FWCI
4.89
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of General Medical Sciences (P50GM098792)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
Keywords
genome editing, synthetic gene circuits, synthetic biology, living cells, information processing in cells
MeSH terms
Gene Editing, CRISPR-Associated Protein 9, RNA, Guide, CRISPR-Cas Systems, Animals, Bacterial Proteins, Brain Mapping, DNA, Endonucleases, Genetic Engineering, Humans, Recombination, Genetic, Biosensing Techniques, Information Storage and Retrieval, Genome, Directed Molecular Evolution, Cell Engineering

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