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Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice
Abstract
Spotting off-targets from gene editing Unintended genomic modifications limit the potential therapeutic use of gene-editing tools. Available methods to find off-targets generally do not work in vivo or detect single-nucleotide changes. Three papers in this issue report new methods for monitoring gene-editing tools in vivo (see the Perspective by Kempton and Qi). Wienert et al. followed the recruitment of a DNA repair protein to DNA breaks induced by CRISPR-Cas9, enabling unbiased detection of off-target editing in cellular and animal models. Zuo et al. identified off-targets without the interference of natural genetic heterogeneity by injecting base editors into one blastomere of a two-cell mouse embryo and leaving the other genetically identical blastomere unedited. Jin et al. performed whole-genome sequencing on individual, genome-edited rice plants to identify unintended mutations. Cytosine, but not adenine, base editors induced numerous single-nucleotide variants in both mouse and rice. Science , this issue p. 286 , p. 289 , p. 292 ; see also p. 234
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“they create new formats of off-target effects such as RNA editing and sgRNA-independent DNA editing”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Jin et al., 2019
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“Although CBE and ABE greatly reduce the classic off-target effects of CRISPR/Cas9 systems”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Jin et al., 2019
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“Previously it has been shown that cytidine but not adenine base editors result in significant, genome-wide, off-target effects57,69.”
From One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids (2023) · cites this paper as [57]
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神先先生の古稀及び定年を迎えられるにあたって (神先秀雄・薄田桂・友松芳郎三教授古稀記念特輯)
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Metadata
- Authors
- Jin, S., Zong, Y., Gao, Q., Zhu, Z., Wang, Y., Qin, P., Liang, C., Wang, D., Qiu, J.-L., Zhang, F., & Gao, C.
- Affiliations
- Chinese Academy of Sciences (CN)
- Sichuan Agricultural University (CN)
- University of Minnesota (US)
- Corresponding author
- Jin, Shuai
- Zong, Yuan
- Gao, Qiang
- Gao, Caixia
- ORCID
- Venue
- Science
- Published
- 2019-04-19
- Volume
- 364
- Issue
- 6437
- Pages
- 292-295
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2917855738
- Cited by
- 665
- References
- 34
- Citations by year
- 2026: 49 · 2025: 59 · 2024: 74 · 2023: 85 · 2022: 91 · 2021: 98 · 2020: 124 · 2019: 83
- FWCI
- 32.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31788103)
- Chinese Academy of Sciences (QYZDY-SSW-SMC030)
- National Key Research and Development Program of China (2016YFD0101804)
- college of biological sciences, University of Minnesota startup funding
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- base editors, cytosine base editors, adenine base editors, off-target mutations, single nucleotide variants, CRISPR/Cas9, genome editing, rice, single-cell whole-genome sequencing
- MeSH terms
- Gene Editing, Whole Genome Sequencing, Adenine, Cytosine, DNA Mutational Analysis, Mutation, Oryza, Polymorphism, Single Nucleotide, Agrobacterium
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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