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Cytosine, but not adenine, base editors induce genome-wide off-target mutations in rice
Abstract
Spotting off-targets from gene editing Unintended genomic modifications limit the potential therapeutic use of gene-editing tools. Available methods to find off-targets generally do not work in vivo or detect single-nucleotide changes. Three papers in this issue report new methods for monitoring gene-editing tools in vivo (see the Perspective by Kempton and Qi). Wienert et al. followed the recruitment of a DNA repair protein to DNA breaks induced by CRISPR-Cas9, enabling unbiased detection of off-target editing in cellular and animal models. Zuo et al. identified off-targets without the interference of natural genetic heterogeneity by injecting base editors into one blastomere of a two-cell mouse embryo and leaving the other genetically identical blastomere unedited. Jin et al. performed whole-genome sequencing on individual, genome-edited rice plants to identify unintended mutations. Cytosine, but not adenine, base editors induced numerous single-nucleotide variants in both mouse and rice. Science , this issue p. 286 , p. 289 , p. 292 ; see also p. 234
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“they create new formats of off-target effects such as RNA editing and sgRNA-independent DNA editing”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Jin et al., 2019
In the citing paper
“Although CBE and ABE greatly reduce the classic off-target effects of CRISPR/Cas9 systems”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Jin et al., 2019
In the citing paper
“Previously it has been shown that cytidine but not adenine base editors result in significant, genome-wide, off-target effects57,69.”
From One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids (2023) · cites this paper as [57]
Citations
Cites
The Sequence Alignment/Map format and SAMtools
Li, H., Handsaker, B., Wysoker, A., Fennell, T., Ruan, J., Homer, N., Marth, G., Abecasis, G., Durbin, R., & 1000 Genome Project Data Processing Subgroup (2009) · Bioinformatics · doi:10.1093/bioinformatics/btp352 · Cited by 62,041
Fast and accurate short read alignment with Burrows-Wheeler transform
Li, H., & Durbin, R. (2009) · Bioinformatics · doi:10.1093/bioinformatics/btp324 · Cited by 58,607
The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data
McKenna, A., Hanna, M., Banks, E., Sivachenko, A., Cibulskis, K., Kernytsky, A., Garimella, K., Altshuler, D., Gabriel, S., Daly, M., et al. (2010) · Genome Research · doi:10.1101/gr.107524.110 · Cited by 27,895
BEDTools: a flexible suite of utilities for comparing genomic features
Quinlan, A. R., & Hall, I. M. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btq033 · Cited by 27,437
HISAT: a fast spliced aligner with low memory requirements
Kim, D., Langmead, B., & Salzberg, S. L. (2015) · Nature Methods · doi:10.1038/nmeth.3317 · Cited by 24,112
Fast and accurate long-read alignment with Burrows-Wheeler transform.
Li, H., & Durbin, R. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btp698 · Cited by 12,063
Cingolani, P., Platts, A., Wang, L. L., Coon, M., Nguyen, T., Wang, L., Land, S. J., Lu, X., & Ruden, D. M. (2012) · Fly · doi:10.4161/fly.19695 · Cited by 11,816
Circos: An information aesthetic for comparative genomicsPreprint
Krzywinski, M. I., Schein, J. E., Birol, İ., Connors, J. M., Gascoyne, R. D., Horsman, D. E., Jones, S. J. M., & Marra, M. A. (2009) · Genome Research · doi:10.1101/gr.092759.109 · Cited by 11,029
Integrative Genomics Viewer (IGV): high-performance genomics data visualization and exploration
Thorvaldsdottir, H., Robinson, J. T., & Mesirov, J. P. (2013) · Briefings in Bioinformatics · doi:10.1093/bib/bbs017 · Cited by 9,049
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437
Flagged first, then by citations
Cited by
Sorted by citations
Items 101–200 of 669
Comprehensive analysis of prime editing outcomes in human embryonic stem cells
Habib, O., Habib, G., Hwang, G., & Bae, S. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab1295 · Cited by 85
Tabassum, J., Ahmad, S., Hussain, B., Mawia, A. M., Zeb, A., & Ju, L. (2021) · Agronomy · doi:10.3390/agronomy11071359 · Cited by 88
Lopes, R., & Prasad, M. K. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1339189 · Cited by 82
Enabling large-scale genome editing at repetitive elements by reducing DNA nicking
Smith, C. J., Castanon, O., Said, K., Volf, V., Khoshakhlagh, P., Hornick, A., Ferreira, R., Wu, C., Güell, M., Garg, S., et al. (2020) · Nucleic Acids Research · doi:10.1093/nar/gkaa239 · Cited by 83
Malabarba, J., Chevreau, E., Dousset, N., Veillet, F., Moizan, J., & Vergne, E. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms22010319 · Cited by 80
Li, C., Zong, Y., Jin, S., Zhu, H., Lin, D., Li, S., Qiu, J., Wang, Y., & Gao, C. (2020) · Genome biology · doi:10.1186/s13059-020-02051-x · Cited by 80
Lee, S., Ding, N., Sun, Y., Yuan, T., Li, J., Yuan, Q., Liu, L., Yang, J., Wang, Q., Kolomeisky, A. B., et al. (2020) · Science Advances · doi:10.1126/sciadv.aba1773 · Cited by 81
CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops
Verma, V., Kumar, A., Partap, M., Thakur, M., & Bhargava, B. (2023) · Frontiers in Plant Science · doi:10.3389/fpls.2023.1122940 · Cited by 84
Cui, Y., Cao, Q., Li, Y., He, M., & Liu, X. (2023) · Journal of Experimental Botany · doi:10.1093/jxb/erad248 · Cited by 77
He, Y., Zhou, X., Chang, C. H., Chen, G., Liu, W., Li, G., Fan, X., Sun, M., Miao, C., Huang, Q., et al. (2024) · Molecular Cell · doi:10.1016/j.molcel.2024.01.021 · Cited by 79
Broadening the GMO risk assessment in the EU for genome editing technologies in agriculture
Kawall, K., Cotter-Howells, J. D., & Then, C. (2020) · Environmental Sciences Europe · doi:10.1186/s12302-020-00361-2 · Cited by 84
Genetic Variation and Unintended Risk in the Context of Old and New Breeding Techniques
Singer, S. D., Laurie, J. D., Bilichak, A., Kumar, S., & Singh, J. (2021) · Critical Reviews in Plant Sciences · doi:10.1080/07352689.2021.1883826 · Cited by 77
Deep learning models to predict the editing efficiencies and outcomes of diverse base editors
Kim, N., Choi, S.-C., Kim, S., Song, M., Seo, J. H., Min, S., Park, J., Cho, S., & Kim, S. (2023) · Nature Biotechnology · doi:10.1038/s41587-023-01792-x · Cited by 78
Precise genome-editing in human diseases: mechanisms, strategies and applications
Zheng, Y., Li, Y., Zhou, K., Li, T., VanDusen, N. J., & Hua, Y. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01750-2 · Cited by 91
Expanding the genome-targeting scope and the site selectivity of high-precision base editors
Tan, J., Zhang, F., Karcher, D., & Bock, R. (2020) · Nature Communications · doi:10.1038/s41467-020-14465-z · Cited by 79
Evolution of plant mutagenesis tools: a shifting paradigm from random to targeted genome editing
Shelake, R. M., Pramanik, D., & Kim, J. (2019) · Plant Biotechnology Reports · doi:10.1007/s11816-019-00562-z · Cited by 79
CRISPR-Cas System: An Approach With Potentials for COVID-19 Diagnosis and Therapeutics
Kumar, P., Malik, Y. S., Ganesh, B., Rahangdale, S., Saurabh, S., Natesan, S., Srivastava, A., Sharun, K., Yatoo, M. I., Tiwari, R., et al. (2020) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2020.576875 · Cited by 76
Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering
Huang, T., & Puchta, H. (2021) · Transgenic Research · doi:10.1007/s11248-021-00238-x · Cited by 77
Principles of and strategies for germline gene therapy
Wolf, D. P., Mitalipov, P. A., & Mitalipov, S. M. (2019) · Nature Medicine · doi:10.1038/s41591-019-0473-8 · Cited by 75
Zhang, E., Neugebauer, M. E., Krasnow, N. A., & Liu, D. R. (2024) · Nature Communications · doi:10.1038/s41467-024-45969-7 · Cited by 68
A Cas-embedding strategy for minimizing off-target effects of DNA base editors
Liu, Y., Zhou, C., Huang, S., Dang, L., Yu, W., He, J., Zhou, Y., Mao, S., Tao, W., Zhang, Y., et al. (2020) · Nature Communications · doi:10.1038/s41467-020-19690-0 · Cited by 70
Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform
Gaillochet, C., Fernández, A. P., Goossens, V., D’Halluin, K., Drozdzecki, A., Shafie, M., Duyse, J. V., Isterdael, G. V., Gonzalez, C., Vermeersch, M., et al. (2023) · Genome biology · doi:10.1186/s13059-022-02836-2 · Cited by 78
Regulation of Shoot Apical Meristem and Axillary Meristem Development in Plants
Xue, Z., Liu, L., & Zhang, C. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21082917 · Cited by 73
Panel), E. P. O. G. M. O. (. G., Naegeli, H., Bresson, J., Dalmay, T., Dewhurst, I. C., Epstein, M. M., Firbank, L. G., Guerche, P., Hejátko, J., Moreno, F. J., et al. (2020) · EFSA Journal · doi:10.2903/j.efsa.2020.6299 · Cited by 77
New prospects on the horizon: Genome editing to engineer plants for desirable traits
Iqbal, Z., Iqbal, M. S., Ahmad, A., Memon, A. G., & Ansari, M. I. (2020) · Current Plant Biology · doi:10.1016/j.cpb.2020.100171 · Cited by 70
Wang, G., Xu, Z., Wang, F., Huang, Y., Xin, Y., Liang, S., Li, B., Si, H., Sun, L., Wang, Q., et al. (2022) · BMC Biology · doi:10.1186/s12915-022-01232-3 · Cited by 71
CRISPR-Cas9 Gene Therapy for Duchenne Muscular Dystrophy
Mbakam, C. H., Lamothe, G., Tremblay, G., & Tremblay, J. P. (2022) · Neurotherapeutics · doi:10.1007/s13311-022-01197-9 · Cited by 81
Genome editing technology and application in soybean improvement
Bao, A., Zhang, C., Huang, Y., Chen, H., Zhou, X., & Cao, D. (2020) · Oil Crop Science · doi:10.1016/j.ocsci.2020.03.001 · Cited by 68
Gene editing and its applications in biomedicine
Li, G., Li, X., Zhuang, S., Wang, L., Zhu, Y., Chen, Y., Sun, W. C., Wu, Z., Zhou, Z. L., Chen, J., et al. (2022) · Science China Life Sciences · doi:10.1007/s11427-021-2057-0 · Cited by 66
Liang, Y., Xie, J., Zhang, Q., Wang, X., Gou, S., Lin, L., Chen, T., Ge, W., Zhuang, Z., Lian, M., et al. (2022) · Nucleic Acids Research · doi:10.1093/nar/gkac353 · Cited by 68
Efficient CRISPR-mediated base editing in Agrobacterium spp.
Rodrigues, S. D., Karimi, M., Impens, L., Lerberge, E. V., Coussens, G., Aesaert, S., Rombaut, D., Holtappels, D., Ibrahim, H. M. M., MONTAGU, M. V., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2013338118 · Cited by 66
Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity
Li, J., Yu, W., Huang, S., Wu, S., Li, L., Zhou, J., Cao, Y., Huang, X., & Qiao, Y. (2021) · Nature Communications · doi:10.1038/s41467-021-22519-z · Cited by 72
One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids
Geurts, M. H., Gandhi, S., Boretto, M. G., Akkerman, N., Derks, L. L. M., van Son, G., Celotti, M., Harshuk-Shabso, S., Peci, F., Begthel, H., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-40701-3 · Cited by 66
The use of new CRISPR tools in cardiovascular research and medicine
Nishiga, M., Liu, C., Qi, L. S., & Wu, J. C. (2022) · Nature Reviews Cardiology · doi:10.1038/s41569-021-00669-3 · Cited by 71
Gao, R., Fu, Z.-C., Li, X., Wang, Y., Wei, J., Li, G., Wang, L., Wu, J., Xue, W., Huang, X., et al. (2022) · The CRISPR Journal · doi:10.1089/crispr.2021.0080 · Cited by 74
Detect-seq reveals out-of-protospacer editing and target-strand editing by cytosine base editors
Lei, Z., Meng, H., Lv, Z., Liu, M., Zhao, H., Wu, H., Zhang, X., Liu, L., Zhuang, Y., Yin, K., et al. (2021) · Nature Methods · doi:10.1038/s41592-021-01172-w · Cited by 67
Perturbing proteomes at single residue resolution using base editing
Després, P. C., Dubé, A. K., Seki, M., Yachie, N., & Landry, C. R. (2020) · Nature Communications · doi:10.1038/s41467-020-15796-7 · Cited by 65
ACBE, a new base editor for simultaneous C-to-T and A-to-G substitutions in mammalian systems
Xie, J., Huang, X., Wang, X., Gou, S., Liang, Y., Chen, F., Li, N., Ouyang, Z., Zhang, Q., Ge, W., et al. (2020) · BMC Biology · doi:10.1186/s12915-020-00866-5 · Cited by 67
Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing
Li, J., Hong, S., Chen, W., Zuo, E., & Yang, H. (2019) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2019.11.002 · Cited by 66
Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation
Wang, X., Liu, Z., Li, G., Dang, L., Huang, S., He, L., Ma, Y., Li, C., Liu, M., Yang, G., et al. (2020) · Molecular Therapy · doi:10.1016/j.ymthe.2019.11.022 · Cited by 89
Applying gene editing to tailor precise genetic modifications in plants
Eck, J. M. V. (2020) · Journal of Biological Chemistry · doi:10.1074/jbc.rev120.010850 · Cited by 69
McGrath, E. L., Shin, H., Zhang, L., Phue, J., Wu, W. W., Shen, R., Jang, Y.-Y., Revollo, J. R., & Ye, Z. (2019) · Nature Communications · doi:10.1038/s41467-019-13342-8 · Cited by 63
Lea, R. A., & Niakan, K. K. (2019) · Nature Cell Biology · doi:10.1038/s41556-019-0424-0 · Cited by 62
The CRISPR/Cas9 revolution continues: From base editing to prime editing in plant science
Li, Y., Li, W., & Li, J. (2021) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2021.05.001 · Cited by 67
CRISPR nuclease off-target activity and mitigation strategies
Wienert, B., & Cromer, M. K. (2022) · Frontiers in Genome Editing · doi:10.3389/fgeed.2022.1050507 · Cited by 66
Annunziato, S., Lutz, C., Henneman, L., Bhin, J., Wong, K., Siteur, B., van Gerwen, B., de Korte‐Grimmerink, R., Zafra, M. P., Schatoff, E. M., et al. (2020) · The EMBO Journal · doi:10.15252/embj.2019102169 · Cited by 62
CRISPR /Cas‐based tools for the targeted control of plant viruses
Robertson, G., Burger, J. T., & Campa, M. (2022) · Molecular Plant Pathology · doi:10.1111/mpp.13252 · Cited by 62
Functional correction of CFTR mutations in human airway epithelial cells using adenine base editors
Krishnamurthy, S., Traore, S., Cooney, A. L., Brommel, C. M., Kulhánková, K., Sinn, P. L., Newby, G. A., Liu, D. R., & McCray, P. B. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab788 · Cited by 63
Huang, W., Zhang, Y., Shen, L., Fang, Q., Liu, Q., Gong, C., Zhang, C., Zhou, Y., Mao, C., Zhu, Y., et al. (2020) · New Phytologist · doi:10.1111/nph.16769 · Cited by 60
Delivery Approaches for Therapeutic Genome Editing and Challenges
Ates, I., Rathbone, T., Stuart, C., Bridges, P. H., & Cottle, R. N. (2020) · Genes · doi:10.3390/genes11101113 · Cited by 63
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Recent advances in therapeutic gene-editing technologies
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Chen, Y., Zhi, S., Liu, W., Wen, J., Hu, S., Cao, T., Sun, H., Li, Y., Huang, L. T., Liu, Y., et al. (2020) · Small Methods · doi:10.1002/smtd.202000309 · Cited by 57
Wu, Y., Ren, Q., Zhong, Z., Liu, G., Han, Y., Bao, Y., Liu, L., Xiang, S., Liu, S., Tang, X., et al. (2022) · Plant Biotechnology Journal · doi:10.1111/pbi.13838 · Cited by 56
CRISPR/Cas systems: opportunities and challenges for crop breeding
Biswas, S., Zhang, D., & Shi, J. (2021) · Plant Cell Reports · doi:10.1007/s00299-021-02708-2 · Cited by 58
Improvement of base editors and prime editors advances precision genome engineering in plants
Hua, K., Han, P., & Zhu, J. (2021) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiab591 · Cited by 62
Predicting base editing outcomes using position-specific sequence determinants
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Cytosine base editor 4 but not adenine base editor generates off-target mutations in mouse embryos
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Molecular mechanisms, off‐target activities, and clinical potentials of genome editing systems
Zheng, N., Li, L., & Wang, X. (2020) · Clinical and Translational Medicine · doi:10.1002/ctm2.34 · Cited by 59
A precise and efficient adenine base editor
Tu, T., Song, Z., Liu, X., Wang, S., He, X., Xi, H., Wang, J., Yan, T., Chen, H., Zhang, Z., et al. (2022) · Molecular Therapy · doi:10.1016/j.ymthe.2022.07.010 · Cited by 55
Jin, S., Lin, Q., Gao, Q., & Gao, C. (2022) · Nature Protocols · doi:10.1038/s41596-022-00773-9 · Cited by 56
Fan, T., Cheng, Y., Wu, Y., Liu, S., Tang, X. D., He, Y., Liao, S., Zheng, X., Zhang, T., Qi, Y., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-49473-w · Cited by 59
Highly efficient single base editing in Aspergillus niger with CRISPR/Cas9 cytidine deaminase fusion
Huang, L., Dong, H., Zheng, J., Wang, B., & Pan, L. (2019) · Microbiological Research · doi:10.1016/j.micres.2019.03.007 · Cited by 59
Cytosine base editing systems with minimized off-target effect and molecular size
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Yuan, T., Yan, N., Fei, T., Zheng, J., Meng, J., Li, N., Liu, J., Zhang, H., Xie, L., Ying, W., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-25217-y · Cited by 55
Methods and applications of CRISPR/Cas system for genome editing in stem cells
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Integrating machine learning and genome editing for crop improvement
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Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions
Liu, Z., Chen, S., Shan, H., Jia, Y., Chen, M., Song, Y., Lai, L., & Li, Z. (2020) · BMC Biology · doi:10.1186/s12915-020-00849-6 · Cited by 53
Crop bioengineering via gene editing: reshaping the future of agriculture
Atia, M. A. M., Jiang, W., Sedeek, K., Butt, H., & Mahfouz, M. M. (2024) · Plant Cell Reports · doi:10.1007/s00299-024-03183-1 · Cited by 59
DNA base editing in nuclear and organellar genomes
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Li, S., & Xia, L. (2019) · aBIOTECH · doi:10.1007/s42994-019-00009-7 · Cited by 55
Precision Breeding Made Real with CRISPR: Illustration through Genetic Resistance to Pathogens
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CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants
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Genome- and transcriptome-wide off-target analyses of an improved cytosine base editor
Randall, L. B., Sretenovic, S., Wu, Y., Yin, D., Zhang, T., Eck, J. M. V., & Qi, Y. (2021) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiab264 · Cited by 51
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Han, W., Qiu, H.-Y., Sun, S., Fu, Z.-C., Wang, G., Qian, X., Wang, L., Zhai, X., Jia, W. P., Wang, Y., et al. (2023) · Cell stem cell · doi:10.1016/j.stem.2023.10.007 · Cited by 53
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Basu, U., Riaz Ahmed, S., Bhat, B. A., Anwar, Z., Ali, A., Ijaz, A., Gulzar, A., Bibi, A., Tyagi, A., Nebapure, S. M., et al. (2023) · Frontiers in Genetics · doi:10.3389/fgene.2022.866976 · Cited by 51
Ethical issues related to research on genome editing in human embryos
Niemiec, E., & Howard, H. C. (2020) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2020.03.014 · Cited by 59
Guyon, A., Rousseau, J., Bégin, F.-G., Bertin, T., Lamothe, G., & Tremblay, J. P. (2021) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2021.02.032 · Cited by 55
Kondo, K., & Taguchi, C. (2022) · Food Safety · doi:10.14252/foodsafetyfscj.d-21-00016 · Cited by 49
Lyu, P., Lu, Z., Cho, S.-I., Yadav, M., Yoo, K. W., Atala, A., Kim, J., & Lu, B. (2021) · The CRISPR Journal · doi:10.1089/crispr.2020.0095 · Cited by 50
Highly efficient base editing with expanded targeting scope using SpCas9‐NG in rabbits
Liu, Z., Shan, H., Chen, S., Chen, M., Song, Y., Lai, L., & Li, Z. (2019) · The FASEB Journal · doi:10.1096/fj.201901587r · Cited by 46
Monsur, M. B., Shao, G., Lv, Y., Ahmad, S., Wei, X., Hu, P., & Tang, S. (2020) · Genes · doi:10.3390/genes11040466 · Cited by 48
Genome editing in the treatment of ocular diseases
Choi, E. H., Suh, S., Sears, A. E., Hołubowicz, R., KEDHAR, S. R., Browne, A., & Palczewski, K. (2023) · Experimental & Molecular Medicine · doi:10.1038/s12276-023-01057-2 · Cited by 51
An adenine base editor variant expands context compatibility
Xiao, Y., Wu, Y., & Tang, W. (2024) · Nature Biotechnology · doi:10.1038/s41587-023-01994-3 · Cited by 48
Current trends in gene recovery mediated by the CRISPR-Cas system
Jang, H., Song, B., Hwang, G., & Bae, S. (2020) · Experimental & Molecular Medicine · doi:10.1038/s12276-020-0466-1 · Cited by 49
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Efficient Correction of a Hypertrophic Cardiomyopathy Mutation by ABEmax-NG
Ma, S., Jiang, W., Liu, X., Lu, W., Qi, T., Wei, J., Wu, F., Chang, Y., Zhang, S., Song, Y., et al. (2021) · Circulation Research · doi:10.1161/circresaha.120.318674 · Cited by 45
Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture
Wang, G., Wang, F., Xu, Z., Wang, Y., Zhang, C., Zhou, Y., Hui, F., Yang, X., Nie, X., Zhang, X., et al. (2024) · Genome biology · doi:10.1186/s13059-024-03189-8 · Cited by 46
Dandage, R., Després, P. C., Yachie, N., & Landry, C. R. (2019) · Genetics · doi:10.1534/genetics.119.302089 · Cited by 46
Multiplex genome editing using a dCas9-cytidine deaminase fusion in Streptomyces
Zhao, Y., Tian, J., Zheng, G., Chen, J., Sun, C., Yang, Z., Zimin, A. A., Jiang, W., Deng, Z., Wang, Z., et al. (2019) · Science China Life Sciences · doi:10.1007/s11427-019-1559-y · Cited by 50
Controllable genome editing with split-engineered base editors
Berríos, K. N., Evitt, N. H., DeWeerd, R. A., Ren, D., Luo, M., Barka, A., Wang, T., Bartman, C., Lan, Y., Green, A. M., et al. (2021) · Nature Chemical Biology · doi:10.1038/s41589-021-00880-w · Cited by 44
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Site-specific CRISPR-based mitochondrial DNA manipulation is limited by gRNA import
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C-to-G editing generates double-strand breaks causing deletion, transversion and translocation
Huang, M. E., Qin, Y., Shang, Y., Hao, Q., Zhan, C., Lian, C., Luo, S., Liu, L. D., Zhang, S., Zhang, Y., et al. (2024) · Nature Cell Biology · doi:10.1038/s41556-023-01342-2 · Cited by 45
Technologies and Computational Analysis Strategies for CRISPR Applications
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CRISPR‐BETS: a base‐editing design tool for generating stop codons
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In Vivo Base Editing of Scn5a Rescues Type 3 Long QT Syndrome in Mice
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Base Editors for Citrus Gene Editing
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Metadata
- Authors
- Jin, S., Zong, Y., Gao, Q., Zhu, Z., Wang, Y., Qin, P., Liang, C., Wang, D., Qiu, J.-L., Zhang, F., & Gao, C.
- Affiliations
- Chinese Academy of Sciences (CN)
- Sichuan Agricultural University (CN)
- University of Minnesota (US)
- Corresponding author
- Jin, Shuai
- Zong, Yuan
- Gao, Qiang
- Gao, Caixia
- ORCID
- Venue
- Science
- Published
- 2019-04-19
- Volume
- 364
- Issue
- 6437
- Pages
- 292-295
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2917855738
- Cited by
- 665
- References
- 34
- Citations by year
- 2026: 49 · 2025: 59 · 2024: 74 · 2023: 85 · 2022: 91 · 2021: 98 · 2020: 124 · 2019: 83
- FWCI
- 32.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31788103)
- Chinese Academy of Sciences (QYZDY-SSW-SMC030)
- National Key Research and Development Program of China (2016YFD0101804)
- college of biological sciences, University of Minnesota startup funding
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Genetic and Mutation Studies
- Keywords
- base editors, cytosine base editors, adenine base editors, off-target mutations, single nucleotide variants, CRISPR/Cas9, genome editing, rice, single-cell whole-genome sequencing
- MeSH terms
- Gene Editing, Whole Genome Sequencing, Adenine, Cytosine, DNA Mutational Analysis, Mutation, Oryza, Polymorphism, Single Nucleotide, Agrobacterium
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