Rapid redistribution of phosphatidylinositol-(4,5)-bisphosphate and septins during the Candida albicans response to caspofungin
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We previously showed that phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] and septin regulation play major roles in maintaining Candida albicans cell wall integrity in response to caspofungin and other stressors. Here, we establish a link between PI(4,5)P2 signaling and septin localization and demonstrate that rapid redistribution of PI(4,5)P2 and septins is part of the natural response of C. albicans to caspofungin. First, we studied caspofungin-hypersusceptible C. albicans irs4 and inp51 mutants, which have elevated PI(4,5)P2 levels due to loss of PI(4,5)P2-specific 5'-phosphatase activity. PI(4,5)P2 accumulated in discrete patches, rather than uniformly, along surfaces of mutants in yeast and filamentous morphologies, as visualized with a green fluorescent protein (GFP)-pleckstrin homology domain. The patches also contained chitin (calcofluor white staining) and cell wall protein Rbt5 (Rbt5-GFP). By transmission electron microscopy, patches corresponded to plasma membrane invaginations that incorporated cell wall material. Fluorescently tagged septins Cdc10 and Sep7 colocalized to these sites, consistent with well-described PI(4,5)P2-septin physical interactions. Based on expression patterns of cell wall damage response genes, irs4 and inp51 mutants were firmly positioned within a group of caspofungin-hypersusceptible, septin-regulatory protein kinase mutants. irs4 and inp51 were linked most closely to the gin4 mutant by expression profiling, PI(4,5)P2-septin-chitin redistribution and other phenotypes. Finally, sublethal 5-min exposure of wild-type C. albicans to caspofungin resulted in redistribution of PI(4,5)P2 and septins in a manner similar to those of irs4, inp51, and gin4 mutants. Taken together, our data suggest that the C. albicans Irs4-Inp51 5'-phosphatase complex and Gin4 function upstream of PI(4,5)P2 and septins in a pathway that helps govern responses to caspofungin.
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Functional Genomic and Chemical Biology Analysis of Echinocandin Resistance in Candida albicans
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Cytoplasmic Membrane Homeostasis is Important for the Pathogenicity of Pathogenic FungiPreprint
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Busch, R. J., Doty, C., Mills, C. A., Latifi, F., Herring, L. E., Konjufca, V. H., Carvallo, F., Boluarte, T., & Vargas-Muñiz, J. M. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.02.25.640155
Septins in the Middle—Makers and Breakers of Membrane Contact Sites
Holt, T. C., & Spiliotis, E. T. (2026) · Journal of Neurochemistry · doi:10.1111/jnc.70495
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- Authors
- Badrane, H., Nguyen, M. H., Blankenship, J. R., Cheng, S., Hao, B., Mitchell, A. P., & Clancy, C. J.
- Affiliations
- University of Pittsburgh (US)
- Carnegie Mellon University (US)
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2012-09
- Volume
- 56
- Issue
- 9
- Pages
- 4614-4624
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC3421880
- OpenAlex
- W2132219294
- Cited by
- 38
- References
- 61
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 5 · 2022: 1 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 2 · 2014: 4 · 2013: 2 · 2012: 1
- FWCI
- 1.16
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Pfizer
- National Institute of Dental and Craniofacial Research (R56DE020831)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- Candida albicans, phosphatidylinositol 4,5-bisphosphate, septins, caspofungin, antifungal susceptibility, cell wall integrity, plasma membrane invaginations, chitin, Cdc10
- MeSH terms
- Caspofungin, Antifungal Agents, Biological Transport, Candida albicans, Cell Wall, Chitin, Fungal Proteins, Phosphoric Monoester Hydrolases, Stress, Physiological, Signal Transduction, Gene Expression Regulation, Fungal, Genes, Reporter, Phosphatidylinositol Phosphates, Cyclin-Dependent Kinases, Green Fluorescent Proteins, Echinocandins, Insulin Receptor Substrate Proteins, Lipopeptides, Septins
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