Cited by 49Bacterial biofilms and quorum sensingAntimicrobial agents and applicationsNanocomposite Films for Food Packaging

Antibacterial Action of Nitric Oxide-Releasing Chitosan Oligosaccharides against Pseudomonas aeruginosa under Aerobic and Anaerobic Conditions

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Reighard, Katelyn P., Schoenfisch, Mark H.·2015·Antimicrobial Agents and Chemotherapy

Abstract

Chitosan oligosaccharides were modified with N -diazeniumdiolates to yield biocompatible nitric oxide (NO) donor scaffolds. The minimum bactericidal concentrations and MICs of the NO donors against Pseudomonas aeruginosa were compared under aerobic and anaerobic conditions. Differential antibacterial activities were primarily the result of NO scavenging by oxygen under aerobic environments and not changes in bacterial physiology. Bacterial killing was also tested against nonmucoid and mucoid biofilms and compared to that of tobramycin. Smaller NO payloads were required to eradicate P. aeruginosa biofilms under anaerobic versus aerobic conditions. Under oxygen-free environments, the NO treatment was 10-fold more effective at killing biofilms than tobramycin. These results demonstrate the potential utility of NO-releasing chitosan oligosaccharides under both aerobic and anaerobic environments.

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  • Biodegradable polymers such as citrate-based or saccharide-based polymers (dextran, chitosan) are attractive scaffolds for NO delivery because of natural occurrence, biodegradability, tolerability to mammalian cells, and accessibility for NO donor functionalization.

    “Lastly, several biodegradable polymers, such as citrate-based225 or saccharide-based polymers (e.g., dextran227,244 and chitosan86,87,220) provide very attractive scaffolds for NO delivery because of their natural occurrence, biodegradability, tolerability to mammalian cells and accessibility for NO donor functionalization reactions.220,244,245”

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Authors
Reighard, K. P., & Schoenfisch, M. H.
Affiliations
University of North Carolina at Chapel Hill (US)
Venue
Antimicrobial Agents and Chemotherapy
Published
2015-10
Volume
59
Issue
10
Pages
6506-6513
ISSN
  • 0066-4804 (ISSN-L)
  • 1098-6596
OpenAlex
W1959736416
Cited by
49
References
62
Citations by year
2026: 2 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 8 · 2020: 8 · 2019: 10 · 2018: 3 · 2017: 4 · 2016: 2 · 2015: 1
FWCI
1.45
Type
journal article
Language
en
Open access
Locations
  • Antimicrobial Agents and Chemotherapy (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Carolina Digital Repository (University of North Carolina at Chapel Hill) (submittedVersion)
  • UNC Libraries
Funders
  • National Institutes of Health
  • University of North Carolina at Chapel Hill
  • National Institute of Allergy and Infectious Diseases (R21AI112029, AI 112029)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
  • Physical Sciences › Materials Science › Biomaterials › Nanocomposite Films for Food Packaging
Keywords
nitric oxide donors, chitooligosaccharides, N-diazeniumdiolates, Pseudomonas aeruginosa, antibacterial activity, biofilm, mucoid biofilm, aerobic and anaerobic conditions, oxygen scavenging, tobramycin, minimum bactericidal concentration
MeSH terms
Aerobiosis, Anaerobiosis, Anti-Bacterial Agents, Azo Compounds, Glycosaminoglycans, Microbial Sensitivity Tests, Nitric Oxide, Oxygen, Pseudomonas aeruginosa, Tobramycin, Biofilms, Nitric Oxide Donors, Chitosan

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