Antifungal Combinations against Candida albicans Biofilms In Vitro
Open accessAbstract
Candida biofilms display increased resistance to most antifungal agents. We have evaluated the efficacy of combinations of fluconazole (FLC), amphotericin B, and caspofungin (CSP) against Candida albicans biofilms in vitro. Indifference was observed for all the combinations of paired antifungal agents when a checkerboard titration method was used. Time-kill experiments revealed an antagonistic effect of high FLC doses with CSP.
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Previous findings have indicated little or no effect for treatment of mature biofilms when FLU is used in combination with other antifungals, including AmB and CAS.
“Previous findings have indicated little or no effect for treatment of mature biofilms when FLU is used in combination with other antifungals, including AmB and CAS [10], whereas synergistic effects (similar to the enhanced effect reported in this study) have been observed when FLU is combined with calcium channel inhibitors [11] and doxycycline [12], among others.”
From In vitro analysis of flufenamic acid activity against Candida albicans biofilms (2014) · cites this paper as [10]
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- Authors
- Bachmann, S. P., Ramage, G., VandeWalle, K., Patterson, T. F., Wickes, B. L., & López-Ribot, J. L.
- Affiliations
- Division of Infectious Diseases, Department of Medicine
- The University of Texas at San Antonio Health Science Center (US)
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2003-11
- Volume
- 47
- Issue
- 11
- Pages
- 3657-3659
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC253764
- OpenAlex
- W2159121924
- Cited by
- 89
- References
- 12
- Citations by year
- 2025: 2 · 2024: 1 · 2021: 3 · 2020: 1 · 2019: 1 · 2018: 2 · 2017: 4 · 2016: 5 · 2015: 6 · 2014: 10 · 2013: 4 · 2012: 6
- FWCI
- 2.72
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- Funders
- National Institute of Dental and Craniofacial Research (5 RO1 DE11381-04, R01DE011381)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
- Keywords
- Candida albicans, biofilm, fluconazole, amphotericin B, caspofungin, checkerboard titration, time-kill kinetics, drug antagonism, antifungal resistance
- MeSH terms
- Caspofungin, Amphotericin B, Antifungal Agents, Candida albicans, Drug Combinations, Peptides, Peptides, Cyclic, Tetrazolium Salts, Fluconazole, Biofilms, Echinocandins, Lipopeptides
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