Cited by 141Phytoestrogen effects and researchGut microbiota and healthBiochemical and biochemical processes

Conversion of daidzein and genistein by an anaerobic bacterium newly isolated from the mouse intestine

Open access
Matthies, Anastasia, Clavel, Thomas, Gütschow, Michael, Engst, Wolfram, Haller, Dirk, Blaut, Michael, Braune, Annett·2008·Applied and Environmental Microbiology

Abstract

The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the Coriobacteriaceae. Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones.

Integrity signals

Registry

Crossref · 10.1128/aem.00555-08

Cites

56 of 56 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

1 of the 57 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Matthies, A., Clavel, T., Gütschow, M., Engst, W., Haller, D., Blaut, M., & Braune, A.
Affiliations
  • German Institute of Human Nutrition (DE)
  • Technical University of Munich (DE)
  • Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, An der Immenburg 4, D-53121 Bonn, Germany
  • Department of Gastrointestinal Microbiology
Corresponding author
Braune, Annett
Venue
Applied and Environmental Microbiology
Published
2008-08
Volume
74
Issue
15
Pages
4847-4852
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2067334985
Cited by
141
References
57
Citations by year
2026: 7 · 2025: 4 · 2024: 16 · 2023: 9 · 2022: 15 · 2021: 9 · 2020: 10 · 2019: 11 · 2018: 5 · 2017: 5 · 2016: 6 · 2015: 8 · 2014: 5 · 2013: 8 · 2012: 6
FWCI
4.02
Type
journal article
Language
en
Open access
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Phytoestrogen effects and research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
Keywords
daidzein, genistein, isoflavone metabolism, equol, dihydrodaidzein, gut microbiota, Coriobacteriaceae, anaerobic bacteria, enzyme induction, soy isoflavones, mouse intestine, metabolic activation
MeSH terms
Animals, Bacteria, Anaerobic, Biotransformation, Chromatography, High Pressure Liquid, Female, Gram-Positive Bacteria, Ileum, Isoflavones, Mice, Inbred C57BL, Molecular Sequence Data, Genistein, Spectrometry, Mass, Electrospray Ionization, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review