Association of Beta-Glucan Endogenous Production with Increased Stress Tolerance of Intestinal Lactobacilli
Open accessAbstract
The exopolysaccharide beta-glucan has been reported to be associated with many health-promoting and prebiotic properties. The membrane-associated glycosyltransferase enzyme (encoded by the gtf gene), responsible for microbial beta-glucan production, catalyzes the conversion of sugar nucleotides into beta-glucan. In this study, the gtf gene from Pediococcus parvulus 2.6 was heterologously expressed in Lactobacillus paracasei NFBC 338. When grown in the presence of glucose (7%, wt/vol), the recombinant strain (pNZ44-GTF(+)) displayed a "ropy" phenotype, while scanning electron microscopy (SEM) revealed strands of polysaccharide-linking neighboring cells. Beta-glucan biosynthesis was confirmed by agglutination tests carried out with Streptococcus pneumoniae type 37-specific antibodies, which specifically detect glucan-producing cells. Further analysis showed a approximately 2-fold increase in viscosity in broth media for the beta-glucan-producing strain over 24 h compared to the control strain, which did not show any significant increase in viscosity. In addition, we analyzed the ability of beta-glucan-producing Lactobacillus paracasei NFBC 338 to survive both technological and gastrointestinal stresses. Heat stress assays revealed that production of the polysaccharide was associated with significantly increased protection during heat stress (60-fold), acid stress (20-fold), and simulated gastric juice stress (15-fold). Bile stress assays revealed a more modest but significant 5.5-fold increase in survival for the beta-glucan-producing strain compared to that of the control strain. These results suggest that production of a beta-glucan exopolysaccharide by strains destined for use as probiotics may afford them greater performance/protection during cultivation, processing, and ingestion. As such, expression of the gtf gene may prove to be a straightforward approach to improve strains that might otherwise prove sensitive in such applications.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Overexpression of genes related to EPS production was demonstrated in S. thermophilus and Lcb. paracasei when stressing conditions are encountered.
“Other studies demonstrated this overexpression of genes related to EPS production in other species such as S. thermophilus [80] and Lcb. paracasei [81] when stressing conditions are encountered.”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [81]
Cites
Hoffman, C. S., & Winston, F. (1987) · Gene · doi:10.1016/0378-1119(87)90131-4 · Cited by 2,492 · see citing works
Probiotics and their fermented food products are beneficial for health
Parvez, S., Malik, K. A., Ah Kang, S., & Kim, H.-Y. (2006) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2006.02963.x · Cited by 1,435 · see citing works
Surviving the Acid Test: Responses of Gram-Positive Bacteria to Low pH
Cotter, P. D., & Hill, C. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.3.429-453.2003 · Cited by 1,378 · see citing works
Controlled gene expression systems for Lactococcus lactis with the food-grade inducer nisin
de Ruyter, P. G. G. A., Kuipers, O. P., & de Vos, W. M. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.10.3662-3667.1996 · Cited by 865 · see citing works
Novel and established applications of microbial polysaccharides
Sutherland, I. W. (1998) · Trends in biotechnology · doi:10.1016/s0167-7799(97)01139-6 · Cited by 678 · see citing works
Stress responses in lactic acid bacteria.
van de Guchte, M., Serror, P., Chervaux, C., Smokvina, T., Ehrlich, S. D., & Maguin, E. (2002) · PubMed · Cited by 668 · see citing works
Stress responses in lactic acid bacteria
van de Guchte, M., Serror, P., Chervaux, C., Smokvina, T., Ehrlich, S. D., & Maguin, E. (2002) · Antonie van Leeuwenhoek · doi:10.1023/a:1020631532202 · Cited by 648 · see citing works
An overview of the functionality of exopolysaccharides produced by lactic acid bacteria
Ruas‐Madiedo, P., Hugenholtz, J., & Zoon, P. (2002) · International Dairy Journal · doi:10.1016/s0958-6946(01)00160-1 · Cited by 625 · see citing works
Protection from gastrointestinal diseases with the use of probiotics
Marteau, P. R., de Vrese, M., Cellier, C., & Schrezenmeir, J. (2001) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.2.430s · Cited by 596 · see citing works
Acid and Bile Tolerance and Cholesterol Removal Ability of Lactobacilli Strains
Liong, M., & Shah, N. P. (2005) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(05)72662-x · Cited by 538 · see citing works
Dietary modulation of immune function by β-glucans
Volman, J. J., Ramakers, J. D., & Plat, J. (2007) · Physiology & Behavior · doi:10.1016/j.physbeh.2007.11.045 · Cited by 523 · see citing works
Water-soluble dietary fibers and cardiovascular disease
Theuwissen, E., & Mensink, R. P. (2008) · Physiology & Behavior · doi:10.1016/j.physbeh.2008.01.001 · Cited by 488 · see citing works
Ruas-Madiedo, P., & de los Reyes-Gavilán, C. G. (2005) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(05)72750-8 · Cited by 456 · see citing works
Market potential for probiotics
Stanton, C., Gardiner, G. E., Meehan, H., Collins, K. J., Fitzgerald, G. F., Lynch, P. B., & Ross, R. P. (2001) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.2.476s · Cited by 416 · see citing works
Self-citationGardiner, G. E., O'Sullivan, E., Kelly, J., Auty, M. A. E., Fitzgerald, G. F., Collins, J. K., Ross, R. P., & Stanton, C. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.6.2605-2612.2000 · Cited by 414 · see citing works
Self-citationEffects of ß-glucans on the immune system
Akramienė, D., Kondrotas, A. J., Didžiapetrienė, J., & Kėvelaitis, E. (2007) · Medicina · doi:10.3390/medicina43080076 · Cited by 377 · see citing works
Bifidobacteria: research and development in Japan.
Ishibashi, N., & Shimamura, S. (1993) · Food technology · Cited by 331 · see citing works
Improved survival of Lactobacillus paracasei NFBC 338 in spray-dried powders containing gum acacia
Desmond, C., Ross, R. P., O’Callaghan, E. J., Fitzgerald, G. F., & Stanton, C. (2002) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2002.01782.x · Cited by 317 · see citing works
Self-citationSurvival of bifidobacteria after spray-drying
Lian, W. (2002) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(01)00733-4 · Cited by 301 · see citing works
Effects of beta-glucans on the immune system.
Akramienė, D., Kondrotas, A. J., Didžiapetrienė, J., & Kėvelaitis, E. (2007) · PubMed · Cited by 297 · see citing works
Overcoming the technological hurdles in the development of probiotic foods
Ross, R. P., Desmond, C., Fitzgerald, G. F., & Stanton, C. (2005) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2005.02654.x · Cited by 296 · see citing works
Self-citationVinderola, G., Perdigón, G., Duarte, J., Farnworth, E. R., & Matar, C. (2006) · Cytokine · doi:10.1016/j.cyto.2007.01.003 · Cited by 291 · see citing works
Biosynthesis, characterisation, and design of bacterial exopolysaccharides from lactic acid bacteria
Laws, A. P., Gu, Y., & Marshall, V. M. (2001) · Biotechnology Advances · doi:10.1016/s0734-9750(01)00084-2 · Cited by 279 · see citing works
International Dairy Federation
Urueña, R. (2010) · Brill | Nijhoff eBooks · doi:10.1163/ej.9789004163300.i-1081.591 · Cited by 256 · see citing works
Desmond, C., Stanton, C., Fitzgerald, G. F., Collins, K. J., & Ross, R. P. (2001) · International Dairy Journal · doi:10.1016/s0958-6946(01)00121-2 · Cited by 244 · see citing works
Self-citation(2006) · Wiley eBooks · doi:10.1002/9780470995785 · Cited by 240 · see citing works
β-Glucans in promoting health: Prevention against mutation and cancer
Mantovani, M. S., Bellini, M. F., Angeli, J. P. F., Oliveira, R. J., Silva, A. F., & Ribeiro, L. R. (2008) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2007.07.002 · Cited by 240 · see citing works
Identification ofLactobacillus plantarumGenes That Are Induced in the Gastrointestinal Tract of Mice
Bron, P. A., Grangette, C., Mercenier, A., de Vos, W. M., & Kleerebezem, M. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.17.5721-5729.2004 · Cited by 239 · see citing works
Korakli, M., Gänzle, M. G., & Vogel, R. F. (2002) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2002.01607.x · Cited by 237 · see citing works
Technology aspects related to microorganisms in functional foods
Knorr, D. (1998) · Trends in Food Science & Technology · doi:10.1016/s0924-2244(98)00051-x · Cited by 235 · see citing works
Prasad, J., McJarrow, P., & Gopal, P. K. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.2.917-925.2003 · Cited by 231 · see citing works
Tsoni, S. V., & Brown, G. D. (2008) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1443.019 · Cited by 207 · see citing works
Kitazawa, H., Harata, T., Uemura, J., Saito, T., Kaneko, T., & Itoh, T. (1998) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(98)00030-0 · Cited by 205 · see citing works
Structural analysis of the exopolysaccharide produced by Pediococcus damnosus 2.6
Dueñas, M. T., Rodríguez-Carvajal, M. Á., Tejero‐Mateo, P., Franco-Rodríguez, G., Espartero, J. L., Irastorza, A., & Serrano, A. M. G. (1997) · Carbohydrate Research · doi:10.1016/s0008-6215(97)00192-4 · Cited by 205 · see citing works
Desmond, C., Fitzgerald, G. F., Stanton, C., & Ross, R. P. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.10.5929-5936.2004 · Cited by 198 · see citing works
Self-citationBegley, M., Gahan, C. G. M., & Hill, C. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.12.6005-6012.2002 · Cited by 196 · see citing works
Life Under Stress: The Probiotic Stress Response and How it may be Manipulated
Corcoran, B., Stanton, C., Fitzgerald, G. F., & Ross, R. P. (2008) · Current Pharmaceutical Design · doi:10.2174/138161208784480225 · Cited by 185 · see citing works
Self-citationSnart, J., Bibiloni, R., Grayson, T., Lay, C., Zhang, H., Allison, G. E., Laverdiere, J. K., Temelli, F., Vasanthan, T., Bell, R. C., et al. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.72.3.1925-1931.2006 · Cited by 174 · see citing works
Effect of orally administered β-glucan on macrophage function in mice
Suzuki, I., Tanaka, H., Kinoshita, A., et al. (1990) · International Journal of Immunopharmacology · doi:10.1016/0192-0561(90)90105-v · Cited by 172 · see citing works
Effects of an exopolysaccharide (kefiran) on lipids, blood pressure, blood glucose, and constipation
Maeda, H., Zhu, X., Omura, K., Suzuki, S., & Kitamura, S. (2004) · BioFactors · doi:10.1002/biof.5520220141 · Cited by 167 · see citing works
Probiotics and inflammatory bowel diseases
Schultz, M. (2000) · The American Journal of Gastroenterology · doi:10.1016/s0002-9270(99)00812-6 · Cited by 165 · see citing works
Characterization of a gram-positive broad-host-range plasmid isolated from Lactobacillus hilgardii
Josson, K., Scheirlinck, T., Michiels, F., Platteeuw, C., Stanssens, P. E., Joos, H., D’Haese, P. C., Zabeau, M., & Mahillon, J. (1989) · Plasmid · doi:10.1016/0147-619x(89)90082-6 · Cited by 143 · see citing works
Role of Nutrients and Bacterial Colonization in the Development of Intestinal Host Defense
Walker, W. A. (2000) · Journal of Pediatric Gastroenterology and Nutrition · doi:10.1097/00005176-200000002-00002 · Cited by 142 · see citing works
Bron, P. A., Marco, M. L., Hoffer, S. M., Mullekom, E. V., de Vos, W. M., & Kleerebezem, M. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.23.7829-7835.2004 · Cited by 139 · see citing works
Corcoran, B. M., Stanton, C., Fitzgerald, G. F., & Ross, R. P. (2006) · Microbiology · doi:10.1099/mic.0.28966-0 · Cited by 136 · see citing works
Self-citationChiu, C.-H., Lu, T.-Y., Tseng, Y., & Pan, T. (2005) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-005-0145-0 · Cited by 128 · see citing works
Assessment of Stress Response of the Probiotic Lactobacillus acidophilus
Kim, W. S., Perl, L., Park, J. H., Tandianus, J. E., & Dunn, N. W. (2001) · Current Microbiology · doi:10.1007/s002840010314 · Cited by 127 · see citing works
Binding of Free Bile Acids by Cells of Yogurt Starter Culture Bacteria
Pigeon, R. M., Cuesta, E. P., & Gilliland, S. E. (2002) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(02)74357-9 · Cited by 110 · see citing works
Tsukada, C., Yokoyama, H., Miyaji, C., Ishimoto, Y., Kawamura, H., & Abo, T. (2003) · Cellular Immunology · doi:10.1016/s0008-8749(03)00061-3 · Cited by 109 · see citing works
van Kranenburg, R., Boels, I. C., Kleerebezem, M., & de Vos, W. M. (1999) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(99)00017-8 · Cited by 107 · see citing works
Bile Affects the Synthesis of Exopolysaccharides by <i>Bifidobacterium animalis</i>
Ruas-Madiedo, P., Gueimonde, M., Arigoni, F., de los Reyes-Gavilán, C. G., & Margolles, A. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.00908-08 · Cited by 103 · see citing works
Structure of an exocellular β-d-glucan from Pediococcus sp., a wine lactic bacteria
Llaubères, R., Richard, B., Lonvaud‐Funel, A., Dubourdieu, D., & Fournet, B. (1990) · Carbohydrate Research · doi:10.1016/0008-6215(90)80049-9 · Cited by 103 · see citing works
Dols‐Lafargue, M., Lee, H. Y., Marrec, C. L., Heyraud, A., Chambat, G., & Lonvaud‐Funel, A. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.00673-08 · Cited by 103 · see citing works
Lee, H.-S., Han, S.-Y., Bae, E., Huh, C., Ahn, Y., Lee, J., & Kim, D. (2008) · International Immunopharmacology · doi:10.1016/j.intimp.2008.01.009 · Cited by 100 · see citing works
Werning, M. L., Ibarburu, I., Dueñas, M. T., Irastorza, A., Navas, J., & López, P. (2006) · Journal of Food Protection · doi:10.4315/0362-028x-69.1.161 · Cited by 97 · see citing works
de Palencia, P. F., Werning, M. L., Sierra‐Filardi, E., Dueñas, M. T., Irastorza, A., Corbí, A. L., & López, P. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.00394-09 · Cited by 95 · see citing works
Walling, E., Gindreau, E., & Lonvaud‐Funel, A. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.05.016 · Cited by 90 · see citing works
Simpson, P. J., Stanton, C., Fitzgerald, G. F., & Ross, R. P. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.2.765-771.2002 · Cited by 85 · see citing works
Self-citationA toxicological assessment of curdlan
Spicer, E. J. F., Goldenthal, E. I., & Ikeda, T. (1999) · Food and Chemical Toxicology · doi:10.1016/s0278-6915(99)00013-7 · Cited by 83 · see citing works
Corcoran, B. M., Ross, R. P., Fitzgerald, G. F., Dockery, P., & Stanton, C. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.02626-05 · Cited by 81 · see citing works
Self-citationLlull, D., Garcı́a, E., & López, R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m010287200 · Cited by 77 · see citing works
Wilson, T. A., Nicolosi, R. J., Chadwell, K., et al. (2004) · Journal of Nutrition · doi:10.1093/jn/134.10.2617 · Cited by 77 · see citing works
Evaluation of the response of Lactobacillus rhamnosus VTT E-97800 to sucrose-induced osmotic stress
Sunny‐Roberts, E. O., & Knorr, D. (2007) · Food Microbiology · doi:10.1016/j.fm.2007.05.003 · Cited by 71 · see citing works
The groESL Chaperone Operon of Lactobacillus johnsonii
Walker, D. C., Girgis, H. S., & Klaenhammer, T. R. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.7.3033-3041.1999 · Cited by 70 · see citing works
Lee, K.-B., Lee, H., & Choi, Y. (2008) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2008.07.1788 · Cited by 66 · see citing works
Role of Nutrients and Bacterial Colonization in the Development of Intestinal Host Defense
Walker, W. A. (2000) · Journal of Pediatric Gastroenterology and Nutrition · doi:10.1097/00005176-200003002-00002 · Cited by 58 · see citing works
The effects of diets enriched in beta-glucans on blood lipoprotein concentrations
Chen, J., & Huang, X. (2009) · Journal of clinical lipidology · doi:10.1016/j.jacl.2009.04.054 · Cited by 51 · see citing works
Effects of Exopolysaccharide-Producing Probiotic Strains on Experimental Colitis in Rats
Şengül, N., Aslím, B., Uçar, G., Yücel, N., Işk, S., Bozkurt, H., Sakaoğullarí, Z., & Atalay, F. (2006) · Diseases of the Colon & Rectum · doi:10.1007/s10350-005-0267-6 · Cited by 50 · see citing works
Antiosteoporotic effect of Polycan, β-glucan from Aureobasidium, in ovariectomized osteoporotic mice
Shin, H. D., Yang, K. J., Park, B. R., Son, C. W., Jang, H. J., & Ku, S. (2007) · Nutrition · doi:10.1016/j.nut.2007.08.011 · Cited by 48 · see citing works
Cytokine induction by a linear 1,3-glucan, curdlan-oligo, in mouse leukocytes in vitro
Hida, T. H., Ishibashi, K., Miura, N. N., Adachi, Y., Shirasu, Y., & Ohno, N. (2009) · Inflammation Research · doi:10.1007/s00011-008-8141-3 · Cited by 46 · see citing works
Fernandez, K., Dueñas, M. T., Irastorza, A., Bilbao, A., & del Campo, G. (1996) · Journal of Food Protection · doi:10.4315/0362-028x-59.1.35 · Cited by 40 · see citing works
Production of Vitamins, Exopolysaccharides and Bacteriocins by Probiotic Bacteria
O’Connor, E. B., Barrett, E., Fitzgerald, G. F., Hill, C., Stanton, C., & Ross, R. P. (2006) · doi:10.1002/9780470995785.ch8 · Cited by 35 · see citing works
Self-citationUse of Probiotic Bacteria for Prevention and Therapy of Allergic Diseases
Felice, G. D., Barletta, B., Butteroni, C., et al. (2008) · Journal of Clinical Gastroenterology · doi:10.1097/mcg.0b013e318169c463 · Cited by 30 · see citing works
Heterologous Expression of a Position 2-Substituted (1→3)-β- d -Glucan in Lactococcus lactis
Werning, M. L., Corrales, M. A., Prieto, A., de Palencia, P. F., Navas, J., & López, P. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.00463-08 · Cited by 30 · see citing works
Microencapsulation of Probiotic Strains for Swine Feeding
Ross, G. R., Gusils, C., & González, S. (2008) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.31.2121 · Cited by 26 · see citing works
(2003) · PubMed · Cited by 24 · see citing works
Gu, Y., Fujimiya, Y., Itokawa, Y., et al. (2008) · Nutrition and Cancer · doi:10.1080/01635580802030884 · Cited by 16 · see citing works
Slime Production by Brewery Strains of Pediococcus Cerevisiae
Oevelen, D. V., & Verachtert, H. (1979) · Journal of the American Society of Brewing Chemists · doi:10.1094/asbcj-37-0034 · Cited by 15 · see citing works
(2008) · PubMed · Cited by 14 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Caggianiello, G., Kleerebezem, M., & Spano, G. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7471-2 · Cited by 396 · see citing works
Ryan, P. M., Ross, R. P., Fitzgerald, G. F., Caplice, N. M., & Stanton, C. (2015) · Food & Function · doi:10.1039/c4fo00529e · Cited by 221 · see citing works
Self-citationBiopolymers from Lactic Acid Bacteria. Novel Applications in Foods and Beverages
Torino, M. I., Font de Valdez, G., & Mozzi, F. (2015) · Frontiers in Microbiology · doi:10.3389/fmicb.2015.00834 · Cited by 201 · see citing works
Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods
Prete, R., Alam, M. K., Perpetuini, G., Perla, C., Pittia, P., & Corsetti, A. (2021) · Foods · doi:10.3390/foods10071653 · Cited by 194 · see citing works
“paracasei [81] when stressing conditions are encountered.”
Beta-Glucans Improve Growth, Viability and Colonization of Probiotic Microorganisms
Russo, P., López, P., Capozzi, V., De Palencia, P. F., Dueñas, M. T., Spano, G., & Fiocco, D. (2012) · International Journal of Molecular Sciences · doi:10.3390/ijms13056026 · Cited by 176 · see citing works
Bove, P., Russo, P., Capozzi, V., Gallone, A., Spano, G., & Fiocco, D. (2013) · Microbiological Research · doi:10.1016/j.micres.2013.01.004 · Cited by 105 · see citing works
Lactobacillus-produced exopolysaccharides and their potential health benefits: A review
Patten, D. A., & Laws, A. P. (2015) · Beneficial Microbes · doi:10.3920/bm2014.0117 · Cited by 100 · see citing works
Kathiriya, M. R., Vekariya, Y. V., & Hati, S. (2023) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-023-10104-3 · Cited by 50 · see citing works
Lindström, C., Holst, O., Nilsson, L., Öste, R., & Andersson, K. E. (2012) · AMB Express · doi:10.1186/2191-0855-2-66 · Cited by 38 · see citing works
Functional food addressing heart health: do we have to target the gut microbiota?
Ryan, P. M., Ross, R. P., Fitzgerald, G. F., Caplice, N. M., & Stanton, C. (2015) · Current Opinion in Clinical Nutrition and Metabolic Care · doi:10.1097/mco.0000000000000224 · Cited by 28 · see citing works
Self-citation
- Authors
- Stack, H. M., Kearney, N., Stanton, C., Fitzgerald, G. F., & Ross, R. P.
- Affiliations
- Teagasc - The Irish Agriculture and Food Development Authority (IE)
- University College Cork (IE)
- Corresponding author
- Ross, R. Paul
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2010-01-15
- Volume
- 76
- Issue
- 2
- Pages
- 500-507
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2805207
- OpenAlex
- W2119656012
- Cited by
- 145
- References
- 79
- Citations by year
- 2026: 3 · 2025: 4 · 2023: 10 · 2022: 6 · 2021: 13 · 2020: 11 · 2019: 10 · 2018: 8 · 2017: 17 · 2016: 7 · 2015: 17 · 2014: 10 · 2013: 5 · 2012: 10
- FWCI
- 12.19
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Teagasc
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Health Sciences › Nursing › Nutrition and Dietetics › Microbial Metabolites in Food Biotechnology
- Keywords
- β-glucan, exopolysaccharides, glycosyltransferases, Lactobacillus paracasei, Pediococcus parvulus, heterologous expression, heat stress, acid stress, bile stress, simulated gastric fluid, viscosity
- MeSH terms
- Bile, Hot Temperature, Intestines, Lactobacillus, Sodium Chloride, Stress, Physiological, Glycosyltransferases, Probiotics, beta-Glucans
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
