The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Mechanisms of Bactericidal Action of Cinnamaldehyde against Listeria monocytogenes and of Eugenol against L. monocytogenes and Lactobacillus sakei
Open accessAbstract
The spice oil components eugenol and cinnamaldehyde possess activity against both gram-positive and gram-negative bacteria, but the mechanisms of action remain obscure. In broth media at 20 degrees C, 5 mM eugenol or 30 mM cinnamaldehyde was bactericidal (>1-log reduction in the number of CFU per milliliter in 1 h) to Listeria monocytogenes. At a concentration of 6 mM eugenol was bactericidal to Lactobacillus sakei, but treatment with 0.5 M cinnamaldehyde had no significant effect. To investigate the role of interference with energy generation in the mechanism of action, the cellular and extracellular ATP levels of cells in HEPES buffer at 20 degrees C were measured. Treatment of nonenergized L. monocytogenes with 5 mM eugenol, 40 mM cinnamaldehyde, or 10 microM carbonyl cyanide m-chlorophenylhydrazone (CCCP) for 5 min prevented an increase in the cellular ATP concentration upon addition of glucose. Treatment of energized L. monocytogenes with 40 mM cinnamaldehyde or 10 microM CCCP caused a rapid decline in cellular ATP levels, but 5 mM eugenol had no effect on cellular ATP. Treatment of L. sakei with 10 mM eugenol prevented ATP generation by nonenergized cells and had no effect on the cellular ATP of energized cells. CCCP at a concentration of 100 microM had no significant effect on the cellular ATP of L. sakei. No significant changes in extracellular ATP were observed. Due to their rapidity, effects on energy generation clearly play a major role in the activity of eugenol and cinnamaldehyde at bactericidal concentrations. The possible mechanisms of inhibition of energy generation are inhibition of glucose uptake or utilization of glucose and effects on membrane permeability.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“An analysis of the intracellular and extracellular ATP levels of cells treated with eugenol, cinnamaldehyde and carvacrol suggested that these compounds might inhibit the ATPase activity of bacterial cells.”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [45]
In the citing paper
“Some components of the EOs, such as eugenol, carvacrol and cinnamaldehyde, are capable of inhibiting the membrane-bound ATPase activity of E. coli and L. monocytogenes.”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [45]
Citations
Cites
Bergey's Manual of Systematic Bacteriology
(1990) · Annals of Internal Medicine · doi:10.7326/0003-4819-112-5-391_3 · Cited by 15,633
Lambert, R. J. W., Skandamis, P. N., Coote, P. J., & Nychas, G.-J. E. (2001) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2001.01428.x · Cited by 2,449
Characterization of the action of selected essential oil components on Gram-negative bacteria
Helander, I. M., Alakomi, H.-L., Latva-Kala, K., Mattila-Sandholm, T., Pol, I., Smid, E. J., Gorris, L. G. M., & von Wright, A. (1998) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf980154m · Cited by 1,664
Ultee, A., Bennik, M. H. J., & Moezelaar, R. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.4.1561-1568.2002 · Cited by 1,602
Preservative Agents in Foods: Mode of Action and Microbial Resistance Mechanisms
Brul, S. (1999) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(99)00072-0 · Cited by 1,041
Juven, B. J., Kanner, J., Schved, F., & Weisslowicz, H. (1994) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1994.tb01661.x · Cited by 833
Mechanisms of Action of Carvacrol on the Food-Borne Pathogen Bacillus cereus
Ultee, A., Kets, E. P. W., & Smid, E. J. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.10.4606-4610.1999 · Cited by 781
ANTIMICROBIAL EFFECTS OF SPICES1
Shelef, L. A. (1984) · Journal of Food Safety · doi:10.1111/j.1745-4565.1984.tb00477.x · Cited by 540
Activity and mechanisms of action of selected biocidal agents on Gram-positive and-negative bacteria
Walsh, S. E., Maillard, J.-Y., Russell, A. D., Catrenich, C. E., Charbonneau, D. L., & Bartolo, R. G. (2003) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2003.01825.x · Cited by 527
Wendakoon, C. N., & Sakaguchi, M. (1995) · Journal of Food Protection · doi:10.4315/0362-028x-58.3.280 · Cited by 377 · cited 3×
“5 mM, histidine decarboxylase) (29) is unlikely because of the rapidity of ATP inhibition or depletion.”
Background
Research works printed but not linked: 2
- Seeliger, H., and D. Jones. 1986. Genus Listeria, p. 1235-1245. In P. H. A. Sneath, N. S. Mair, M. E. Sharpe, and J. G. Holt (ed.), Bergey's manual of systematic bacteriology vol. 2. Williams and Wilkins, Baltimore, Md.
- Shelef, L. A. 1983. Antimicrobial effects of spices. J. Food Saf.6:29-44.
Cited by
Sorted by citations
Items 401–441 of 441
Romero‐Gil, V., Garrido-Fernández, A., & Arroyo‐López, F. N. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.01370 · Cited by 1
Essential oils as modifiers of rumen metabolism and reducers of methane gas production
Cruz, A., Pineda, C., & Moreno, G. (2023) · Veterinarska stanica · doi:10.46419/vs.55.4.1 · Cited by 1
The effects of sugar substitution with cinnamon powder in moringa jelly candy
Sadek, N. F., & Lie, D. B. (2024) · IOP Conference Series Earth and Environmental Science · doi:10.1088/1755-1315/1338/1/012026 · Cited by 1
IN-VITRO ANTIBACTERIAL EFFICACY OF COMMON SPICES AND THEIR EFFECT ON PERMEABILITY OF MEMBRANE
Vani, K., Lakshmi, O. B., Naidu, S. D., & Maha, V. (2014) · International Journal of Pharma and Bio Sciences · Cited by 1
Kukla, R., Mazurová, J., Krovakova, I., Slehová, E., Sleha, R., Rozkot, M., & Opletal, L. (2019) · Military Medical Science Letters · doi:10.31482/mmsl.2019.018 · Cited by 1
Chen, C. H., Marchello, J. A., Friedman, M., & Ravishankar, S. (2021) · Food and Nutrition Sciences · doi:10.4236/fns.2021.122014 · Cited by 1
Phytochemicals as Antimicrobial Agents
Oladele, O. T., Aladejana, O. M., Adewole, T. S., Olowookere, B. D., & Oladele, J. O. (2022) · Advances in medical diagnosis, treatment, and care (AMDTC) book series · doi:10.4018/978-1-6684-5129-8.ch011 · Cited by 1
Ma, Y.-H. (2016) · TSpace (University of Toronto) · Cited by 1
Adams, C. (2020) · ScholarWorks@UMassAmherst (University of Massachusetts Amherst) · doi:10.7275/6052814.0
Effects of Water Activity and Smoke Compounds on the Growth of Listeria monocytogenes in Raw Ham
Goto, S., Watanabe, I., Omori, Y., Fuchu, H., Miake, K., Morioka, Y., Kotani, K., Kosai, K., Kamisaki-Horikoshi, N., Sameshima, T., et al. (2014) · NIPPON SHOKUHIN KOGYO GAKKAISHI · doi:10.3136/nskkk.61.9
Hernández, S., & Jaime, M. C. (2014)
Pulan, S., Kaplan, S., & Ulusoy, S. (2015) · DergiPark (Istanbul University)
Therapie der unkomplizierten Zystitis der Frau ohne Antibiotika
el Zellner, M. (2018) · gynäkologie + geburtshilfe · doi:10.1007/s15013-018-1430-1
Rebh, M. Y., & Alhasi, S. M. (2020) · Mağallaẗ al-ʿulūm wa-al-dirāsāt al-insāniyyaẗ. · doi:10.37376/1571-000-034-009
Shi, C. (2018) · The Atrium (University of Guelph)
Zheng, Y., Mao, Y.-D., Zhang, L.-P., & Jin, Q. (2020) · IOP Conference Series Earth and Environmental Science · doi:10.1088/1755-1315/559/1/012023
Akiyama, Y., Kondo, H., Kimura, S., & Fujii, Y. (1995) · PubMed
Do, J., In, M., & Kim, D. C. (2022) · Journal of Applied Biological Chemistry · doi:10.3839/jabc.2022.024
Cinnamon oil value addition in natural rubber latex‐organic peroxide vulcanization system
Abeygunawardana, H. N. A. B., Perera, I. C., & Kuruwita‐Mudiyanselage, T. D. (2024) · Journal of Applied Polymer Science · doi:10.1002/app.56447
In vitro inhibition of the bacterial honey bee pathogen Melissococcus plutonius with essential oils
Çifçioğlu, F. N., Kaplan, E., Özakar, E., Öz, C., Özakar, R. S., Kaplan, M. F., Cengiz, S., & Adıgüzel, M. C. (2025) · Journal of Apicultural Research · doi:10.1080/00218839.2025.2483014
Khongsai, L., & Singh, C. B. (2025) · Springer eBooks · doi:10.1007/978-981-96-4640-1_8
Abdelaziz, A. M., Imane, A., & Mustapha, B. (2025) · E3S Web of Conferences · doi:10.1051/e3sconf/202563201001
Antimicrobial Activity of Clove Oil Extract against A. baumanii and its Impact on Gene Expression
Al-Dulaimi, T. H., Abdul-Husin, I. F., Al-Musawi, A. M.-S., Al-Jayid, R. D., Kamil, S. S., Kataa, B. R., Sabr, R. A., Ibraheam, I. A., & Al-Bermani, O. K. (2025) · Medical Journal of Babylon · doi:10.4103/mjbl.mjbl_666_24
El-Gamal, S. M. A., Rashad, E. M., Saber, W. I. A., Al-Askar, A. A., Helmy, Y. A., Ghoneem, K. M., & Ibrahim, A. A. (2025) · Physiology and Molecular Biology of Plants · doi:10.1007/s12298-025-01650-x
Lepoutre, A., Debonne, E., Genechten, W. V., Martini, S., Dijck, P. V., & Devlieghere, F. (2025) · Foods · doi:10.3390/foods14213604
Pourkhosravani, E., Dehghan-Nayeri, F., & Mohammadi Bazargani, M. (2021) · Figshare · doi:10.6084/m9.figshare.16880156
Do, D. N., & Xuan-Tien, L. E. (2026) · Sustainable Chemistry One World · doi:10.1016/j.scowo.2025.100179
The possibilities of treating inflammatory diseases of the throat with herbal preparations
Ruzhentsova, T. A., Александрович, Г. А., Bayrakova, A. L., & Popova, I. A. (2026) · Medical alphabet · doi:10.33667/2078-5631-2025-29-50-54
H. Bouchiha, R. R. (2015) · International Journal of Biosciences (IJB) · doi:10.12692/ijb/7.4.150-156
Kumar, D., Srivastava, V., & Das, D. (2026) · Polymer Bulletin · doi:10.1007/s00289-026-06603-2
Veilumuthu, P., Ramanujam, S., Karthiga, A. R., Subburaj, S., Narayanan, A. B., S, G. V. G., Roy, R., Nair, S. V. S., G, A. A., Rajan, Y. A. P. A. S., et al. (2026) · Next research. · doi:10.1016/j.nexres.2026.102438
Singh, S., Pathak, V., Goswami, M., Venkatesh, A. K. T., Rajkumar, V., & Kumari, R. (2026) · Journal of Food Science and Technology · doi:10.1007/s13197-026-06927-x
Alaasam, B., Alnabari, Y., & Huleihel, M. (2026) · Cells · doi:10.3390/cells15191829
The efficacy of antimicrobial paperboard coated with clove oil in modified starch carriers.
Vanit, S., Suppakul, P., & Jinkarn, T. (2010)
Metadata
- Authors
- Gill, A. O., & Holley, R. A.
- Affiliations
- University of Manitoba (CA)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-10
- Volume
- 70
- Issue
- 10
- Pages
- 5750-5755
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC522076
- OpenAlex
- W2135642891
- Cited by
- 439
- References
- 30
- Citations by year
- 2026: 11 · 2025: 18 · 2024: 24 · 2023: 22 · 2022: 21 · 2021: 28 · 2020: 30 · 2019: 31 · 2018: 35 · 2017: 18 · 2016: 31 · 2015: 31 · 2014: 28 · 2013: 27 · 2012: 18
- FWCI
- 9.40
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Sciences and Engineering Research Council of Canada
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antimicrobial agents and applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Listeria monocytogenes in Food Safety
- Keywords
- cinnamaldehyde, eugenol, Listeria monocytogenes, Lactobacillus sakei, bactericidal mechanism, intracellular ATP, extracellular ATP, CCCP, glucose uptake, membrane permeability
- MeSH terms
- Acrolein, Adenosine Triphosphate, Anti-Bacterial Agents, Energy Metabolism, Eugenol, Flavoring Agents, Food Microbiology, Glucose, Lactobacillus, Listeria monocytogenes, Plant Oils
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
