The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Mechanisms of Bactericidal Action of Cinnamaldehyde against Listeria monocytogenes and of Eugenol against L. monocytogenes and Lactobacillus sakei
Open accessAbstract
The spice oil components eugenol and cinnamaldehyde possess activity against both gram-positive and gram-negative bacteria, but the mechanisms of action remain obscure. In broth media at 20 degrees C, 5 mM eugenol or 30 mM cinnamaldehyde was bactericidal (>1-log reduction in the number of CFU per milliliter in 1 h) to Listeria monocytogenes. At a concentration of 6 mM eugenol was bactericidal to Lactobacillus sakei, but treatment with 0.5 M cinnamaldehyde had no significant effect. To investigate the role of interference with energy generation in the mechanism of action, the cellular and extracellular ATP levels of cells in HEPES buffer at 20 degrees C were measured. Treatment of nonenergized L. monocytogenes with 5 mM eugenol, 40 mM cinnamaldehyde, or 10 microM carbonyl cyanide m-chlorophenylhydrazone (CCCP) for 5 min prevented an increase in the cellular ATP concentration upon addition of glucose. Treatment of energized L. monocytogenes with 40 mM cinnamaldehyde or 10 microM CCCP caused a rapid decline in cellular ATP levels, but 5 mM eugenol had no effect on cellular ATP. Treatment of L. sakei with 10 mM eugenol prevented ATP generation by nonenergized cells and had no effect on the cellular ATP of energized cells. CCCP at a concentration of 100 microM had no significant effect on the cellular ATP of L. sakei. No significant changes in extracellular ATP were observed. Due to their rapidity, effects on energy generation clearly play a major role in the activity of eugenol and cinnamaldehyde at bactericidal concentrations. The possible mechanisms of inhibition of energy generation are inhibition of glucose uptake or utilization of glucose and effects on membrane permeability.
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“An analysis of the intracellular and extracellular ATP levels of cells treated with eugenol, cinnamaldehyde and carvacrol suggested that these compounds might inhibit the ATPase activity of bacterial cells.”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [45]
In the citing paper
“Some components of the EOs, such as eugenol, carvacrol and cinnamaldehyde, are capable of inhibiting the membrane-bound ATPase activity of E. coli and L. monocytogenes.”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [45]
Citations
Cites
Bergey's Manual of Systematic Bacteriology
(1990) · Annals of Internal Medicine · doi:10.7326/0003-4819-112-5-391_3 · Cited by 15,633
Lambert, R. J. W., Skandamis, P. N., Coote, P. J., & Nychas, G.-J. E. (2001) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2001.01428.x · Cited by 2,449
Characterization of the action of selected essential oil components on Gram-negative bacteria
Helander, I. M., Alakomi, H.-L., Latva-Kala, K., Mattila-Sandholm, T., Pol, I., Smid, E. J., Gorris, L. G. M., & von Wright, A. (1998) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf980154m · Cited by 1,664
Ultee, A., Bennik, M. H. J., & Moezelaar, R. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.4.1561-1568.2002 · Cited by 1,602
Preservative Agents in Foods: Mode of Action and Microbial Resistance Mechanisms
Brul, S. (1999) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(99)00072-0 · Cited by 1,041
Juven, B. J., Kanner, J., Schved, F., & Weisslowicz, H. (1994) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1994.tb01661.x · Cited by 833
Mechanisms of Action of Carvacrol on the Food-Borne Pathogen Bacillus cereus
Ultee, A., Kets, E. P. W., & Smid, E. J. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.10.4606-4610.1999 · Cited by 781
ANTIMICROBIAL EFFECTS OF SPICES1
Shelef, L. A. (1984) · Journal of Food Safety · doi:10.1111/j.1745-4565.1984.tb00477.x · Cited by 540
Activity and mechanisms of action of selected biocidal agents on Gram-positive and-negative bacteria
Walsh, S. E., Maillard, J.-Y., Russell, A. D., Catrenich, C. E., Charbonneau, D. L., & Bartolo, R. G. (2003) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2003.01825.x · Cited by 527
Wendakoon, C. N., & Sakaguchi, M. (1995) · Journal of Food Protection · doi:10.4315/0362-028x-58.3.280 · Cited by 377 · cited 3×
“5 mM, histidine decarboxylase) (29) is unlikely because of the rapidity of ATP inhibition or depletion.”
BackgroundUse of firefly luciferase in atp-related assays of biomass, enzymes, and metabolites
Lundin, A. (2000) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(00)05499-9 · Cited by 274
ANTONY VAN LEEUWENHOEK AND HIS “LITTLE ANIMALS.”
Dobell, C. (1933) · The American Journal of the Medical Sciences · doi:10.1097/00000441-193308000-00024 · Cited by 260
Antibacterial activity of essential oil components
Moleyar, V., & Narasimham, P. (1992) · International Journal of Food Microbiology · doi:10.1016/0168-1605(92)90035-2 · Cited by 224
Bactericidal activity of carvacrol towards the food-borne pathogen Bacillus cereus
Ultee, Gorris, L. G. M., & Smid (1998) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.1998.00467.x · Cited by 217
Blaszyk, M. (1998) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(97)00134-7 · Cited by 185
Natural Antimicrobials for the Minimal Processing of Foods
Roller, S. C. 2. /. J. (2003) · CRC Press eBooks · doi:10.1201/9780203508244 · Cited by 160
Antimicrobials from herbs and spices
Nychas, G. E., Skandamis, P. N., & Tassou, C. C. (2003) · Elsevier eBooks · doi:10.1533/9781855737037.176 · Cited by 150
Bacteriocidal effects and inhibition of cell separation of cinnamic aldehyde on Bacillus cereus
Kwon, J. A., Yu, C. B., & Park, H. D. (2003) · Letters in Applied Microbiology · doi:10.1046/j.1472-765x.2003.01350.x · Cited by 118
ANTIMICROBIAL ACTIVITY OF SOME FOOD FLAVORING COMPOUNDS
Jay, J. M., & RIVERS, G. M. (1984) · Journal of Food Safety · doi:10.1111/j.1745-4565.1984.tb00609.x · Cited by 111
Carbohydrate Utilization in Lactobacillus sake
Lauret, R., Morel-Deville, F., Berthier, F., Vergès, M.-C. C., Postma, P. W., Ehrlich, S. D., & Zagorec, M. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.6.1922-1927.1996 · Cited by 96
Parker, V. (1965) · PubMed · Cited by 62
Antibotulinal Properties of Selected Aromatic and Aliphatic Aldehydes
Bowles, B. L., & Miller, A. J. (1993) · Journal of Food Protection · doi:10.4315/0362-028x-56.9.788 · Cited by 60
INHIBITORY EFFECTS OF FLAVOR COMPOUNDS ON STAPHYLOCOCCUS AUREUS WRRC B1241
Bowles, B. L., Sackitey, S., & Williams, A. C. (1995) · Journal of Food Safety · doi:10.1111/j.1745-4565.1995.tb00144.x · Cited by 50
Shabala, L., Budde, B. B., Ross, T., Siegumfeldt, H., Jakobsen, M., & McMeekin, T. A. (2002) · Applied and Environmental Microbiology · doi:10.1128/aem.68.4.1794-1802.2002 · Cited by 47
Rico-Munoz, E., Bargiota, E. E., & Davidson, P. M. (1987) · Food Microbiology · doi:10.1016/0740-0020(87)90006-2 · Cited by 41
Ishida, A., Yoshikawa, T., Nakazawa, T., & Kamidate, T. (2002) · Analytical Biochemistry · doi:10.1006/abio.2002.5680 · Cited by 32
Nisin in multifactorial food preservation
Adams, M., & Smid, E. J. (2003) · Elsevier eBooks · doi:10.1533/9781855737037.11 · Cited by 29
Parker, C., & Hutkins, R. W. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.2.543-546.1997 · Cited by 26
Post-process, packaging-induced fermentation of delicatessen meats
Holley, R. A., Doyon, G., Fortin, J., Rodrigue, N., & Carbonneau, M. (1996) · Food Research International · doi:10.1016/0963-9969(95)00055-0 · Cited by 12
Self-citationNaturally occurring antimicrobial systems
Roller, S. D., & R. G. Board (2003) · Springer eBooks · doi:10.1007/978-0-387-30042-9_13 · Cited by 8
Research works printed but not linked: 2
- Seeliger, H., and D. Jones. 1986. Genus Listeria, p. 1235-1245. In P. H. A. Sneath, N. S. Mair, M. E. Sharpe, and J. G. Holt (ed.), Bergey's manual of systematic bacteriology vol. 2. Williams and Wilkins, Baltimore, Md.
- Shelef, L. A. 1983. Antimicrobial effects of spices. J. Food Saf.6:29-44.
Cited by
Sorted by citations
Hyldgaard, M., Mygind, T., & Meyer, R. L. (2012) · Frontiers in Microbiology · doi:10.3389/fmicb.2012.00012 · Cited by 2,191
Effect of essential oils on pathogenic bacteria
Nazzaro, F., Fratianni, F., De Martino, L., Coppola, R., & De Feo, V. (2013) · Pharmaceuticals · doi:10.3390/ph6121451 · Cited by 2,186
“Cinnamaldehyde is generally less powerful than eugenol [45], but when it is used against E.”
BackgroundNegi, P. S. (2012) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2012.03.006 · Cited by 687
Synergy between essential oil components and antibiotics: a review
Langeveld, W. T., Veldhuizen, E. J. A., & Burt, S. A. (2013) · Critical Reviews in Microbiology · doi:10.3109/1040841x.2013.763219 · Cited by 629
Chemical Preservatives and Natural Antimicrobial Compounds
Davidson, P. M., Taylor, T. M., & Schmidt, S. E. (2014) · ASM Press eBooks · doi:10.1128/9781555818463.ch30 · Cited by 572
Metadata
- Authors
- Gill, A. O., & Holley, R. A.
- Affiliations
- University of Manitoba (CA)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2004-10
- Volume
- 70
- Issue
- 10
- Pages
- 5750-5755
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC522076
- OpenAlex
- W2135642891
- Cited by
- 439
- References
- 30
- Citations by year
- 2026: 11 · 2025: 18 · 2024: 24 · 2023: 22 · 2022: 21 · 2021: 28 · 2020: 30 · 2019: 31 · 2018: 35 · 2017: 18 · 2016: 31 · 2015: 31 · 2014: 28 · 2013: 27 · 2012: 18
- FWCI
- 9.40
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Sciences and Engineering Research Council of Canada
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antimicrobial agents and applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Listeria monocytogenes in Food Safety
- Keywords
- cinnamaldehyde, eugenol, Listeria monocytogenes, Lactobacillus sakei, bactericidal mechanism, intracellular ATP, extracellular ATP, CCCP, glucose uptake, membrane permeability
- MeSH terms
- Acrolein, Adenosine Triphosphate, Anti-Bacterial Agents, Energy Metabolism, Eugenol, Flavoring Agents, Food Microbiology, Glucose, Lactobacillus, Listeria monocytogenes, Plant Oils
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