Fate of Escherichia coli during Ensiling of Wheat and Corn
Open accessAbstract
A recombinant Escherichia coli strain carrying a plasmid with an antibiotic resistance marker and expressing the green fluorescent protein was inoculated at a concentration of 3.8 x 10(8) CFU/g into direct-cut wheat (348 g of dry matter kg(-1)), wilted wheat (450 g of dry matter kg(-1)), and corn (375 g of dry matter kg(-1)). The forages were ensiled in mini-silos. The treatments included control (no E. coli added), application of tagged E. coli, and delayed sealing of the inoculated wheat. Three silos per treatment were sampled on predetermined dates, and the numbers of E. coli were determined on Chromocult TBX medium with or without kanamycin. Colonies presumptively identified as E. coli were also tested for fluorescence activity. Addition of E. coli at the time of ensiling resulted in a more rapid decrease in the pH but had almost no effect on the chemical composition of the final silages or their aerobic stability. E. coli disappeared from the silages when the pH decreased below 5.0. It persisted longer in silages of wilted wheat, in which the pH declined more slowly. Control silages of all crops also contained bacteria, presumptively identified as E. coli, that were resistant to the antibiotic, which suggests that some epiphytic strains are naturally resistant to antibiotics.
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E. coli O157:H7 was eliminated from ensiled, contaminated wheat and corn forage when pH fell below 5.0.
“Escherichia coli O157:H7 was eliminated from ensiled, contaminated wheat and corn forage when the pH decreased below 5.0 (Chen et al., 2005).”
From Control of Escherichia coli O157:H7 in corn silage with or without various inoculants: Efficacy and mode of action (2010) · cites this paper as Chen et al., 2005
All studies reviewed indicate that ensiling in well-managed silages efficiently eliminates E. coli O157:H7.
“Nevertheless, all studies we reviewed on the subject indicate that the ensiling process in well-managed silages is efficient at eliminating E. coli O157:H7 (Bach et al., 2002;Byrne et al., 2002;Chen et al., 2005).”
From Control of Escherichia coli O157:H7 in corn silage with or without various inoculants: Efficacy and mode of action (2010) · cites this paper as Chen et al., 2005
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- Authors
- Chen, Y., Sela, S., Gamburg, M., Pinto, R., & Weinberg, Z. G.
- Affiliations
- Agricultural Research Organization (IL)
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2005-09
- Volume
- 71
- Issue
- 9
- Pages
- 5163-5170
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC1214677
- OpenAlex
- W2004283815
- Cited by
- 27
- References
- 15
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 2 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 3 · 2013: 4
- FWCI
- 2.63
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- Escherichia coli, silage inoculation, wheat silage, corn silage, silage pH, aerobic stability, silage chemical composition, antibiotic resistance, green fluorescent protein, wilted forage, epiphytic bacteria, silage microbiology
- MeSH terms
- Zea mays, Culture Media, Escherichia coli, Fermentation, Hydrogen-Ion Concentration, Recombinant Fusion Proteins, Silage, Triticum, Colony Count, Microbial, Drug Resistance, Bacterial, Green Fluorescent Proteins
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