Persistence of Escherichia coli O157:H7 in barley silage: Effect of a bacterial inoculant
Abstract
AIMS: The effect of a lactic acid producing bacterial (LAB) inoculant on the elimination of Escherichia coli O157:H7 from barley forage was assessed. METHODS AND RESULTS: Triplicate mini-silos were prepared for four treatments and six sampling times (1, 3, 7, 15, 30 and 42 d post-ensiling). The treatments were (i) 10(5) cfu g(-1) Pediococcus pentosaceus and Propionibacterium jenzenii (P2); (ii) 10(5) cfu g(-1) E. coli O157:H7 strain 3081 and 10(5) cfu g(-1) E. coli Biotype 1 strains 719IE10, 719IE14 and 614ME49 (EC); (iii) P2 + EC; and (iv) the control (sterile distilled water). Triplicate mini-silos were opened at each sampling time for pH, volatile fatty acid (VFA) and lactate determinations and E. coli, E. coli O157:H7 and LAB were enumerated. On d 3 and 7, numbers of E. coli O157:H7 in P2 + EC were significantly lower than in EC (P < 0;05). Escherichia coli O157:H7 was not detected in P2 + EC and EC at 7 and 15 d post-ensiling, respectively. On d 15 through 42, E. coli Biotype 1 was not detected in P2 + EC or EC. Populations of LAB were higher in P2 and P2 + EC than in the control and EC on d 3 and 7 (P < 0.05). After 3 d of ensiling, lactate levels were higher (P < 0.05) and pH was lower (P < 0.05) in P2 and P2 + EC as compared to the control and EC. Bacteriocins of P2 were not found to be inhibitory to E. coli O157:H7 using the agar-spot procedure. Escherichia coli O157:H7 inoculated into the control silage at a level of 10(3) cfu g(-1) and exposed to aerobic conditions at 22 degrees C was not detected after 1 d and remained undetectable for the 28 d exposure period. CONCLUSIONS: Silage inoculant P2 increased lactate levels and decreased pH more rapidly during ensiling, which appeared to hasten the elimination of E. coli O157:H7 from the silage. SIGNIFICANCE AND IMPACT OF THE STUDY: Results emphasize the importance of adequate ensiling since E. coli O157:H7 may be maintained and spread among cattle through feed.
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Treatment with a bacterial inoculant that accelerated the pH decrease hastened elimination of the pathogen.
“Elimination of the pathogen has been hastened by treatment with a bacterial inoculant that accelerated the pH decrease (Bach et al., 2002).”
From Control of Escherichia coli O157:H7 in corn silage with or without various inoculants: Efficacy and mode of action (2010) · cites this paper as Bach et al., 2002
All studies reviewed indicate that ensiling in well-managed silages efficiently eliminates E. coli O157:H7.
“Nevertheless, all studies we reviewed on the subject indicate that the ensiling process in well-managed silages is efficient at eliminating E. coli O157:H7 (Bach et al., 2002;Byrne et al., 2002;Chen et al., 2005).”
From Control of Escherichia coli O157:H7 in corn silage with or without various inoculants: Efficacy and mode of action (2010) · cites this paper as Bach et al., 2002
Cites
Hemorrhagic Colitis Associated with a Rare Escherichia coli Serotype
Riley, L. W., Remis, R. S., Helgerson, S. D., McGee, H. B., Wells, J. G., DAVIS, B. R., Hebert, R. J., Olcott, E. S., Johnson, L., Hargrett, N. T., et al. (1983) · New England Journal of Medicine · doi:10.1056/nejm198303243081203 · Cited by 2,669 · see citing works
Griffin, P. M., & Tauxe, R. V. (1991) · Epidemiologic Reviews · doi:10.1093/oxfordjournals.epirev.a036079 · Cited by 1,824 · see citing works
Elder, R., Keen, J., Siragusa, G., Barkocy-Gallagher, G. A., Koohmaraie, M., & Laegreid, W. (2000) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.97.7.2999 · Cited by 952 · see citing works
Wells, J. G., Shipman, L. D., Greene, K. D., Sowers, E. G., Green, J. H., Cameron, D. N., Downes, F. P., Martin, M. L., Griffin, P. M., & Ostroff, S. M. (1991) · Journal of Clinical Microbiology · doi:10.1128/jcm.29.5.985-989.1991 · Cited by 518 · see citing works
A 1-year study of Escherichia coli O157 in cattle, sheep, pigs and poultry
CHAPMAN, P. A., Siddons, C. A., MALO, A. T. C., & HARKIN, M. A. (1997) · Epidemiology and Infection · doi:10.1017/s0950268897007826 · Cited by 444 · see citing works
The prevalence ofEscherichia coliO157.H7 in dairy and beef cattle in Washington State
Hancock, D. D., Besser, T. E., Kinsel, M. L., Tarr, P. I., Rice, D. H., & Paros, M. G. (1994) · Epidemiology and Infection · doi:10.1017/s0950268800051633 · Cited by 399 · see citing works
Prevalence of enterohemorrhagic Escherichia coli O157:H7 in a survey of dairy herds
Zhao, T., Doyle, M. P., Shere, J. A., & Garber, L. P. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.4.1290-1293.1995 · Cited by 398 · see citing works
Acid tolerance of enterohemorrhagic Escherichia coli
Benjamin, M. M., & Datta, A. R. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.4.1669-1672.1995 · Cited by 346 · see citing works
Cai, Y., Benno, Y., Ogawa, M., & Kumai, S. (1999) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(99)75263-x · Cited by 328 · see citing works
Spelhaug, S. R., & Harlander, S. K. (1989) · Journal of Food Protection · doi:10.4315/0362-028x-52.12.856 · Cited by 196 · see citing works
Survival of verocytotoxigenicEscherichia coliO157 in soil, water and on surfaces
Maule, A. F. (2000) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2000.tb05334.x · Cited by 183 · see citing works
Kudo, H., Cheng, K.-J., & Costerton, J. W. (1987) · Canadian Journal of Microbiology · doi:10.1139/m87-041 · Cited by 166 · see citing works
Elder, R. O. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.060024897 · Cited by 161 · see citing works
The fate ofEscherichia coliandE. coliO157 in cattle slurry after application to land
Fenlon, D. R., Ogden, I. D., Vinten, A. J. A., & Svoboda, I. F. (2000) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2000.tb05343.x · Cited by 133 · see citing works
Gansheroff, L. J., & O’Brien, A. D. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.7.2959 · Cited by 118 · see citing works
Chateau, N., Castellanos, I., & Deschamps, A. M. (1993) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1993.tb02993.x · Cited by 100 · see citing works
The Occurrence and Replication of Escherichia coli in Cattle Feeds
Lynn, T. V., Hancock, D. D., Besser, T. E., Harrison, J. H., Rice, D. H., Stewart, N. T., & Rowan, L. L. (1998) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(98)75672-3 · Cited by 98 · see citing works
McAllister, T. A., Selinger, L. B., McMahon, L. R., Bae, H. D., Lysyk, T. J., Oosting, S. J., & Cheng, K.-J. (1995) · Canadian Journal of Animal Science · doi:10.4141/cjas95-062 · Cited by 79 · see citing works
Assessment of Pediococcus acidilactici as a Potential Silage Inoculant
Fitzsimons, A., Duffner, F. M., Curtin, D., Brophy, G., O’Kiely, P., & O'Connell, M. (1992) · Applied and Environmental Microbiology · doi:10.1128/aem.58.9.3047-3052.1992 · Cited by 52 · see citing works
Fenlon, D. R., & Wilson, J. C. (2000) · Letters in Applied Microbiology · doi:10.1046/j.1472-765x.2000.00679.x · Cited by 50 · see citing works
Bacteria in manure and on manured and NPK-fertilised silage crops
Östling, C. E., & Lindgren, S. E. (1991) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2740550409 · Cited by 47 · see citing works
The influence of bacterial inoculants on the microbial ecology of aerobic spoilage of barley silage
Inglis, G. D., Yanke, L. J., Kawchuk, L. M., & McAllister, T. A. (1999) · Canadian Journal of Microbiology · doi:10.1139/w98-207 · Cited by 38 · see citing works
The numbers of epiphytic bacteria on grass at ensilage on commercial farms
Rooke, J. A. (1990) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.2740510409 · Cited by 26 · see citing works
(2000) · Cited by 22 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Enterohaemorrhagic Escherichia coli : emerging issues on virulence and modes of transmission
Caprioli, A., Morabito, S., Brug�re, H., & Oswald, É. (2005) · Veterinary Research · doi:10.1051/vetres:2005002 · Cited by 667 · see citing works
Silage processing and strategies to prevent persistence of undesirable microorganisms.
Dunière, L., Sindou, J., Chaucheyras-Durand, F., Chevallier, I., & Thévenot-Sergentet, D. (2013) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2013.04.006 · Cited by 335 · see citing works
Silage review: Animal and human health risks from silage
Driehuis, F., Wilkinson, J. M., Jiang, Y., Ogunade, I. M., & Adesogan, A. T. (2018) · Journal of Dairy Science · doi:10.3168/jds.2017-13836 · Cited by 265 · see citing works
Silage review: Foodborne pathogens in silage and their mitigation by silage additives
Queiroz, O. C. M., Ogunade, I. M., Weinberg, Z., & Adesogan, A. T. (2018) · Journal of Dairy Science · doi:10.3168/jds.2017-13901 · Cited by 213 · see citing works
Diet,Escherichia coliO157:H7, and Cattle: A Review After 10 Years
Callaway, T. R., Carr, M. A., Edrington, T. S., Anderson, R. C., & Nisbet, D. J. (2009) · Current Issues in Molecular Biology · doi:10.21775/cimb.011.067 · Cited by 190 · see citing works
Silage and the safety and quality of dairy foods: A review
Driehuis, F. (2013) · Agricultural and Food Science · doi:10.23986/afsci.6699 · Cited by 150 · see citing works
Transmission and control of Escherichia coli O157:H7 — A review
Bach, S. J., McAllister, T. A., Veira, D. M., Gannon, V. P. J., & Holley, R. A. (2002) · Canadian Journal of Animal Science · doi:10.4141/a02-021 · Cited by 146 · see citing works
Ogunade, I., Jiang, Y., Kim, D.-H., Pech-Cervantes, A., Arriola, K., Vyas, D., Weinberg, Z., Jeong, K., & Adesogan, A. (2017) · Journal of Dairy Science · doi:10.3168/jds.2016-11745 · Cited by 88 · see citing works
Berry, E. D., & Wells, J. E. (2010) · Advances in food and nutrition research · doi:10.1016/s1043-4526(10)60004-6 · Cited by 84 · see citing works
Wang, X., Haruta, S., Wang, P., Ishii, M., Igarashi, Y., & Cui, Z. (2006) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2006.00099.x · Cited by 83 · see citing works
Land application of treated sewage sludge: quantifying pathogen risks from consumption of crops
Gale, P. (2005) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2004.02482.x · Cited by 81 · see citing works
Selection of tropical lactic acid bacteria for enhancing the quality of maize silage
Santos, A. O., Ávila, C. L. S., & Schwan, R. F. (2013) · Journal of Dairy Science · doi:10.3168/jds.2013-6782 · Cited by 72 · see citing works
Control of Escherichia coli O157:H7 in contaminated alfalfa silage: Effects of silage additives.
Ogunade, I. M., Kim, D. H., Jiang, Y., Weinberg, Z. G., Jeong, K. C., & Adesogan, A. T. (2016) · Journal of Dairy Science · doi:10.3168/jds.2015-10766 · Cited by 66 · see citing works
Chaves, A. V., Baah, J., Wang, Y., McAllister, T. A., & Benchaar, C. (2011) · Journal of the Science of Food and Agriculture · doi:10.1002/jsfa.4669 · Cited by 45 · see citing works
Pedroso, A. F., Adesogan, A. T., Queiroz, O. C. M., & Williams, S. K. (2010) · Journal of Dairy Science · doi:10.3168/jds.2009-2433 · Cited by 43 · see citing works
Reduction in Fusarium Toxin Levels in Corn Silage with Low Dry Matter and Storage Time
Boudra, H., & Morgavi, D. P. (2008) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf800267k · Cited by 39 · see citing works
The microflora of fermented nixtamalized corn
Sefa‐Dedeh, S., Cornelius, B., Amoa‐Awua, W. K., Sakyi‐Dawson, E., & Afoakwa, E. O. (2004) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(03)00114-4 · Cited by 28 · see citing works
Fate of <i>Escherichia coli</i> during Ensiling of Wheat and Corn
Chen, Y., Sela, S., Gamburg, M., Pinto, R., & Weinberg, Z. G. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.9.5163-5170.2005 · Cited by 27 · see citing works
The effect of sewage irrigation on safety and hygiene of forage crops and silage
Weinberg, Z. G., Ashbell, G., Chen, Y., Gamburg, M., & Sela, S. (2004) · Animal Feed Science and Technology · doi:10.1016/j.anifeedsci.2004.07.009 · Cited by 26 · see citing works
Callaway, T. R., Edrington, T. S., Loneragan, G. H., Carr, M. A., & Nisbet, D. J. (2013) · Cited by 25 · see citing works
Dunière, L., Gleizal, A., Chaucheyras‐Durand, F., Chevallier, I., & Thévenot-Sergentet, D. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.06320-11 · Cited by 24 · see citing works
Ravva, S. V., & Korn, A. M. (2007) · Applied and Environmental Microbiology · doi:10.1128/aem.02213-06 · Cited by 22 · see citing works
Arasu, M. V., Jung, M., Kim, D., Park, H. S., Soundharrajan, I., Al-Dhabi, N. A., & Choi, K. C. (2014) · Annals of Microbiology · doi:10.1007/s13213-014-0830-2 · Cited by 22 · see citing works
Utilization of Sugarcane Industrial Residues as Animal Food and Probiotic Medium
Apás, A. L., Arena, M. E., Draksler, D., & González, S. (2008) · Journal of Bioscience and Bioengineering · doi:10.1263/jbb.106.363 · Cited by 17 · see citing works
Barley forages for modern global ruminant agriculture: A review
Nikkhah, A. (2013) · Russian Agricultural Sciences · doi:10.3103/s1068367413030038 · Cited by 14 · see citing works
Passive immunization by recombinant ferric enterobactin protein (FepA) from Escherichia coli O157.
Larrie-Bagha, S. M., Rasooli, I., Gargari, S. L. M., Rasooli, Z., & Nazarian, S. (2013) · Europe PMC (PubMed Central) · Cited by 13 · see citing works
Fluctuations in the occurrence of Escherichia coli O157:H7 on a Norwegian farm*
Wasteson, Y., Johannessen, G. S., Bruheim, T., Urdahl, A. M., O'Sullivan, K., & Rørvik, L. M. (2005) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2005.01673.x · Cited by 12 · see citing works
Perspectives on Food-Safety Issues of Animal-Derived Foods
(2010) · University of Arkansas Press eBooks · doi:10.2307/j.ctt1ffjftf · Cited by 9 · see citing works
Prevalence and Molecular Detection of Intimin (eaeA) Virulence Gene in E. coli O157:H7 in Calves
Yousif, A. A. (2015) · Research Journal for Veterinary Practitioners · doi:10.14737/journal.rjvp/2015/3.3.47.52 · Cited by 8 · see citing works
Nyambe, S., Burgess, C., Whyte, P., O'Kiely, P., & Bolton, D. (2017) · Journal of Applied Microbiology · doi:10.1111/jam.13431 · Cited by 7 · see citing works
Avery, S. M., Walters, L. D., & Hutchison, M. L. (2005) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2004.01640.x · Cited by 6 · see citing works
Shiga toxin-producing Escherichia coli, faecal coliforms and coliphage in animal feeds
Hutchison, M. L., Thomas, D. J. I., Walters, L. D., & Avery, S. M. (2006) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.2006.01929.x · Cited by 5 · see citing works
Verotoxinogenic Escherichia coli O157:H7 in Swedish cattle and pigs
Eriksson, E. (2010) · Epsilon Open Archive (Sveriges lantbruksuniversitet biblioteket (Swedish University of Agricultural Sciences)) · Cited by 4 · see citing works
Arroyo‐López, F. N., Bautista‐Gallego, J., Romero‐Gil, V., Baquero, J., Garcı́a, P., Jiménez‐Díaz, R., López‐López, A., Rodríguez‐Gómez, F., & Garrido-Fernández, A. (2012) · CRC Press eBooks · doi:10.1201/b12055-20 · Cited by 3 · see citing works
Fané, S., Vale, P., Bajón-Fernández, Y., Cartmell, E., Nocker, A., Harris, J., & Tyrrel, S. (2019) · Waste and Biomass Valorization · doi:10.1007/s12649-019-00865-w · Cited by 3 · see citing works
Perez, H. G., Seidel, D. S., & Callaway, T. R. (2025) · Foodborne Pathogens and Disease · doi:10.1177/15353141251386418 · Cited by 2 · see citing works
Fate of inoculated Escherichia coli in hay
Weinberg, Z. G., Chen, Y., Pinto, R., & Sela, S. (2006) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2006.03192.x · Cited by 2 · see citing works
Evaluating the microbial safety status of products from sustainable organic agriculture
Elumalai, S. D. (2016) · doi:10.31274/etd-180810-4737 · Cited by 2 · see citing works
Vegetable Fermentation with Economical Significance in South America
Arena, M. E., Apás, A. L., & González, S. (2012) · CRC Press eBooks · doi:10.1201/b12055-24 · Cited by 1 · see citing works
Wu, B., Cao, X., Fu, M., Bao, Y., Wu, T., Liu, K., Wen, S., Gao, F., Wang, H., Mei, H., et al. (2025) · Agronomy · doi:10.3390/agronomy15081888
Il'ina, L. A., Филиппова, В., Yildirim, E. A., Sklyarov, S., & Laptev, G. Y. (2025) · Lecture notes in networks and systems · doi:10.1007/978-3-031-98127-2_37
- Authors
- Bach, S. J., McAllister, T. A., Baah, J., Yanke, L. J., Veira, D. M., Gannon, V. P. J., & Holley, R. A.
- Affiliations
- Agriculture and Agri-Food Canada (CA)
- Health Canada (CA)
- University of Manitoba (CA)
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2002-08
- Volume
- 93
- Issue
- 2
- Pages
- 288-294
- ISSN
- 1364-5072 (ISSN-L)
- 1365-2672
- OpenAlex
- W1982251046
- Cited by
- 42
- References
- 24
- Citations by year
- 2025: 3 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 1 · 2013: 6 · 2012: 2
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Listeria monocytogenes in Food Safety
- Keywords
- Escherichia coli O157:H7, barley silage, lactic acid bacteria inoculant, Pediococcus pentosaceus, lactate, silage pH, volatile fatty acids, bacteriocins
- MeSH terms
- Aerobiosis, Animals, Hordeum, Cattle, Fatty Acids, Volatile, Feces, Silage, Colony Count, Microbial, Lactic Acid, Escherichia coli O157
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