Fate of Escherichia coli O157:H7 during silage fermentation
Open accessAbstract
The survival characteristics of Escherichia coli O157:H7 in silage derived from contaminated grass were investigated. The survival of other enteric bacteria was also investigated to determine if E. coli O157:H7 demonstrates enhanced acid tolerance in comparison. Samples of chopped grass were treated as follows: (i) no additive (control); (ii) inoculation with E. coli O157:H7 to a final concentration of log10 4.0 CFU g(-1); (iii) addition of an 85% solution of formic acid at 3.0 ml kg(-1) grass; and (iv) addition of both E. coli O157:H7 and formic acid, at the above concentrations. Treated 6-kg grass samples were packed into laboratory silos, sealed, and stored at 15 degrees C for up to 180 days. Individual replicate silos were removed from storage periodically and subjected to microbiological and chemical analyses. Chemical analyses of the silage samples indicated that lactic acid-dominant fermentations, with a rapid drop in pH, occurred. Numbers of enteric bacteria decreased from log10 7.0 to 8.0 CFU g(-1) to undetectable levels within 19 days' storage. E. coli O157:H7 did not survive the silage fermentation process, with numbers declining from approximately log10 4.0 CFU g(-1) to undetectable levels within 19 days of ensiling. The pattern of decline in numbers of E. coli O157:H7 was the same as that for the enteric bacteria, indicating that under the conditions tested, the acid tolerance of E. coli O157:H7 was not significantly different from the acid tolerance of other enteric bacteria. This study found that E. coli O157:H7 did not survive a good silage fermentation process, indicating that properly ensiled grass that is correctly stored is unlikely to be a vector for the transmission of the pathogen among cattle.
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All studies reviewed indicate that ensiling in well-managed silages efficiently eliminates E. coli O157:H7.
“Nevertheless, all studies we reviewed on the subject indicate that the ensiling process in well-managed silages is efficient at eliminating E. coli O157:H7 (Bach et al., 2002;Byrne et al., 2002;Chen et al., 2005).”
From Control of Escherichia coli O157:H7 in corn silage with or without various inoculants: Efficacy and mode of action (2010) · cites this paper as Byrne et al., 2002
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Self-citation
- Authors
- Byrne, C. M., O'kiely, P., Bolton, D. J., Sheridan, J. J., Mcdowell, D. A., & Blair, I. S.
- Affiliations
- Teagasc - The Irish Agriculture and Food Development Authority (IE)
- University of Ulster (GB)
- Corresponding author
- Byrne, C.M.
- Venue
- Journal of Food Protection
- Publisher
- Elsevier BV
- Published
- 2002-12
- Volume
- 65
- Issue
- 12
- Pages
- 1854-1860
- ISSN
- 0362-028X (ISSN-L)
- OpenAlex
- W121377907
- Cited by
- 28
- References
- 31
- Citations by year
- 2025: 1 · 2021: 2 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 1 · 2013: 4
- FWCI
- 1.04
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Food Protection (publishedVersion)
- PubMed (publishedVersion)
- Funders
- European Commission
- Teagasc
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Listeria monocytogenes in Food Safety
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Keywords
- Escherichia coli O157:H7, silage fermentation, acid resistance, enteric bacteria, formic acid, silage additives, lactic acid fermentation, pathogen survival, cattle feed contamination, pathogen transmission
- MeSH terms
- Fermentation, Food Contamination, Food Handling, Food Microbiology, Food Preservation, Hydrogen-Ion Concentration, Lactobacillus, Silage, Time Factors, Escherichia coli O157
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