Activation of an Essential Calcium Signaling Pathway in Saccharomyces cerevisiae by Kch1 and Kch2, Putative Low-Affinity Potassium Transporters

Stefan, Christopher P.; Zhang, Nannan; Sokabe, Takaaki; Rivetta, Alberto; Slayman, Clifford L.; Montell, Craig; Cunningham, Kyle W.

doi:10.1128/ec.00299-12

Cited by 32Ion channel regulation and functionFungal and yeast genetics researchIon Channels and Receptors

Activation of an Essential Calcium Signaling Pathway in Saccharomyces cerevisiae by Kch1 and Kch2, Putative Low-Affinity Potassium Transporters

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Stefan, Christopher P., Zhang, Nannan, Sokabe, Takaaki, Rivetta, Alberto, Slayman, Clifford L., Montell, Craig, Cunningham, Kyle W.·2013·Eukaryotic Cell

DOI 10.1128/ec.00299-12PMC PMC3571297

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Abstract

In the budding yeast Saccharomyces cerevisiae , mating pheromones activate a high-affinity Ca 2+ influx system (HACS) that activates calcineurin and is essential for cell survival. Here we identify extracellular K + and a homologous pair of transmembrane proteins, Kch1 and Kch2 (Prm6), as necessary components of the HACS activation mechanism. Expression of Kch1 and especially Kch2 was strongly induced during the response to mating pheromones. When forcibly overexpressed, Kch1 and Kch2 localized to the plasma membrane and activated HACS in a fashion that depended on extracellular K + but not pheromones. They also promoted growth of trk1 trk2 mutant cells in low K + environments, suggesting they promote K + uptake. Voltage-clamp recordings of protoplasts revealed diminished inward K + currents in kch1 kch2 double-mutant cells relative to the wild type. Conversely, heterologous expression of Kch1 in HEK293T cells caused the appearance of inwardly rectifying K + currents. Collectively, these findings suggest that Kch1 and Kch2 directly promote K + influx and that HACS may electrochemically respond to K + influx in much the same way as the homologous voltage-gated Ca 2+ channels in most animal cell types.

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An upstream activator of calcineurin (KCH1) in S. cerevisiae and C. albicans was present on the list of 36 genes with low tolerance/persistence Z-scores but was not grouped in the calcineurin ontology.
“An upstream activator of calcineurin (KCH1) in S. cerevisiae and C. albicans (34, 35) was also present on this list, but was not grouped in this ontology.”
From Echinocandin tolerance and persistence in vitro are regulated by calcineurin signaling in Candida glabrata (2026) · cites this paper as [34]

Methods in yeast genetics
Sherman, F., Fink, G. R., & Hicks, J. B. (1979) · Cold Spring Harbor Laboratory eBooks · Cited by 7,543 · see citing works
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
Longtine, M. S., Mckenzie, A., DeMarini, D. J., Shah, N. G., Wach, A., Brachat, A., Philippsen, P., & Pringle, J. R. (1998) · Yeast · doi:10.1002/(sici)1097-0061(199807)14:10 · Cited by 5,367 · see citing works
Methods in Yeast Genetics
Jacob, H. (1988) · Bioelectrochemistry and Bioenergetics · doi:10.1016/0302-4598(88)80052-7 · Cited by 4,911 · see citing works
Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles
Roberts, C. J., Nelson, B., Marton, M. J., Stoughton, R. B., Meyer, M. R., Bennett, H. A., He, Y. D., Dai, H. A., Walker, W. L., Hughes, T. R., et al. (2000) · Science · doi:10.1126/science.287.5454.873 · Cited by 855 · see citing works
Ion Channel Voltage Sensors: Structure, Function, and Pathophysiology
Catterall, W. A. (2010) · Neuron · doi:10.1016/j.neuron.2010.08.021 · Cited by 570 · see citing works
A new family of outwardly rectifying potassium channel proteins with two pore domains in tandem
Ketchum, K. A., Joiner, W. J., Sellers, A. J., Kaczmarek, L. K., & Goldstein, S. A. N. (1995) · Nature · doi:10.1038/376690a0 · Cited by 439 · see citing works
Calcineurin-dependent growth control in Saccharomyces cerevisiae mutants lacking PMC1, a homolog of plasma membrane Ca2+ ATPases
Cunningham, K. W., & Fink, G. R. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.124.3.351 · Cited by 432 · see citing works
Self-citation
Calcineurin Inhibits VCX 1-Dependent H + /Ca 2 + Exchange and Induces Ca 2 + ATPases in Saccharomyces cerevisiae
Cunningham, K. W., & Fink, G. R. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.5.2226 · Cited by 431 · see citing works
Self-citation
The protein phosphatase calcineurin is essential for NaCl tolerance of Saccharomyces cerevisiae.
Mendoza, I., Rubio, F., Rodriguez-Navarro, A., & Pardo, J. M. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)37038-2 · Cited by 350 · see citing works
Harnessing calcineurin as a novel anti-infective agent against invasive fungal infections.
Steinbach, W. J., Reedy, J. L., Cramer, R. A., Perfect, J. R., & Heitman, J. (2007) · Nature Reviews Microbiology · doi:10.1038/nrmicro1680 · Cited by 313 · see citing works
Yeast has homologs (CNA1 and CNA2 gene products) of mammalian calcineurin, a calmodulin-regulated phosphoprotein phosphatase
Cyert, M. S., Kunisawa, R., Kaim, D., & Thorner, J. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.16.7376 · Cited by 289 · see citing works
TRK1 and TRK2 encode structurally related K+ transporters in Saccharomyces cerevisiae.
Ko, C. H., & Gaber, R. F. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.8.4266 · Cited by 284 · see citing works
Essential role of calcineurin in response to endoplasmic reticulum stress
Bonilla, M., Nastase, K. K., & Cunningham, K. W. (2002) · The EMBO Journal · doi:10.1093/emboj/21.10.2343 · Cited by 255 · see citing works
Self-citation
The relationship between ATP and an electrogenic pump in the plasma membrane ofNeurospora crassa
Slayman, C. L., Long, W. S., & Lu, C. Y. (1973) · The Journal of Membrane Biology · doi:10.1007/bf01868083 · Cited by 247 · see citing works
Self-citation
Regulatory subunit (CNB1 gene product) of yeast Ca2+/calmodulin-dependent phosphoprotein phosphatases is required for adaptation to pheromone.
Cyert, M., & Thorner, J. W. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.8.3460 · Cited by 236 · see citing works
Independent sorting-out of thousands of duplicated gene pairs in two yeast species descended from a whole-genome duplication.
Scannell, D. R., Frank, A. C., Conant, G. C., Byrne, K. P., Woolfit, M., & Wolfe, K. H. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0608218104 · Cited by 235 · cited 1× · see citing works
“The homologous YJR054w and PRM6 genes, hereafter designated KCH1 and KCH2, respectively, arose from a whole-genome duplication event in an ancestral species of yeast (36) and are predicted to encode closely related transmembrane proteins.”
Background
Essential role for induced Ca2+ influx followed by [Ca2+]i rise in maintaining viability of yeast cells late in the mating pheromone response pathway. A study of [Ca2+]i in single Saccharomyces cerevisiae cells with imaging of fura-2.
Iida, H., Yagawa, Y., & Anraku, Y. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)38311-5 · Cited by 224 · see citing works
MID1, a novel Saccharomyces cerevisiae gene encoding a plasma membrane protein, is required for Ca2+ influx and mating.
Iida, H., Nakamura, H., Ono, T., Okumura, M. S., & Anraku, Y. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.12.8259 · Cited by 218 · see citing works
A Homolog of Voltage-Gated Ca 2+ Channels Stimulated by Depletion of Secretory Ca 2+ in Yeast
Locke, E. G., Bonilla, M., Liang, L., Takita, Y., & Cunningham, K. W. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.18.6686-6694.2000 · Cited by 212 · see citing works
Self-citation
Characterization of the Calcium-mediated Response to Alkaline Stress in Saccharomyces cerevisiae
Viladevall, L., Serrano, R., Ruiz, A., Doménech, G., Giraldo, J., Barceló, A., & Ariño, J. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m403606200 · Cited by 207 · see citing works
12 Pheromone Response and Signal Transduction during the Mating Process of Saccharomyces cerevisiaePreprint
Sprague, G. F., & Thorner, J. W. (1992) · Cold Spring Harbor Monograph Archive · doi:10.1101/087969365.21b.657 · Cited by 201 · see citing works
A Homolog of Mammalian, Voltage-Gated Calcium Channels Mediates Yeast Pheromone-Stimulated Ca 2+ Uptake and Exacerbates the cdc1 (Ts) Growth Defect
Paidhungat, M., & Garrett, S. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.11.6339 · Cited by 191 · see citing works
Ectopic Potassium Uptake in trk1 trk2 Mutants ofSaccharomyces cerevisiae Correlates with a Highly Hyperpolarized Membrane Potential
Madrid, R., Gómez, M. J., Ramos, J., & Rodríguez‐Navarro, A. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.24.14838 · Cited by 190 · see citing works
How might the diversity of potassium channels be generated?
Jan, L. Y., & Jan, Y. N. (1990) · Trends in Neurosciences · doi:10.1016/0166-2236(90)90123-r · Cited by 188 · see citing works
The Saccharomyces cerevisiae CCH1 gene is involved in calcium influx and mating
Fischer, M., Schnell, N., Chattaway, J., Davies, P., Dixon, G., & Sanders, D. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)01466-x · Cited by 185 · see citing works
Prm1p, a Pheromone-Regulated Multispanning Membrane Protein, Facilitates Plasma Membrane Fusion during Yeast Mating
Heiman, M. G., & Walter, P. (2000) · The Journal of Cell Biology · doi:10.1083/jcb.151.3.719 · Cited by 183 · see citing works
Yeast Respond to Hypotonic Shock with a Calcium Pulse
Batiza, A. F., Schulz, T., & Masson, P. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.38.23357 · Cited by 178 · see citing works
Functional Genomic Analysis of Fluconazole Susceptibility in the Pathogenic Yeast Candida glabrata : Roles of Calcium Signaling and Mitochondria
Kaur, R., Castaño, I., & Cormack, B. P. (2004) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.48.5.1600-1613.2004 · Cited by 167 · cited 1× · see citing works
“cerevisiae and Candida glabrata cells during exposure to azole-class compounds (18, 35, 49).”
Background
Structural Models of the KtrB, TrkH, and Trk1,2 Symporters Based on the Structure of the KcsA K+ Channel
Durell, S. R., & Guy, H. R. (1999) · Biophysical Journal · doi:10.1016/s0006-3495(99)76932-8 · Cited by 165 · see citing works
Electrical Properties of Neurospora crassa
Slayman, C. L. (1965) · The Journal of General Physiology · doi:10.1085/jgp.49.1.69 · Cited by 165 · see citing works
Self-citation
Mitogen-activated Protein Kinase Stimulation of Ca2+Signaling Is Required for Survival of Endoplasmic Reticulum Stress in Yeast
Bonilla, M., & Cunningham, K. W. (2003) · Molecular Biology of the Cell · doi:10.1091/mbc.e03-02-0113 · Cited by 165 · see citing works
Self-citation
Hyphal Orientation of Candida albicans Is Regulated by a Calcium-Dependent Mechanism
Brand, A., Shanks, S., Duncan, V. M. S., Yang, M., Mackenzie, K. S., & Gow, N. A. R. (2007) · Current Biology · doi:10.1016/j.cub.2006.12.043 · Cited by 154 · see citing works
Differential Regulation of Two Ca2+ Influx Systems by Pheromone Signaling in Saccharomyces cerevisiae
Muller, E. M., Locke, E., & Cunningham, K. W. (2001) · Genetics · doi:10.1093/genetics/159.4.1527 · Cited by 133 · see citing works
Self-citation
The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5.Preprint
Kranz, J. E., Satterberg, B., & Elion, E. A. (1994) · Genes & Development · doi:10.1101/gad.8.3.313 · Cited by 130 · see citing works
Acidic calcium stores of Saccharomyces cerevisiae
Cunningham, K. W. (2011) · Cell Calcium · doi:10.1016/j.ceca.2011.01.010 · Cited by 120 · see citing works
Self-citation
Multiple Signaling Pathways Regulate Yeast Cell Death during the Response to Mating Pheromones
Zhang, N.-N., Dudgeon, D. D., Paliwal, S., Levchenko, A., Grote, E., & Cunningham, K. W. (2006) · Molecular Biology of the Cell · doi:10.1091/mbc.e06-03-0177 · Cited by 115 · see citing works
Self-citation
Fig1p Facilitates Ca2+ Influx and Cell Fusion during Mating of Saccharomyces cerevisiae
Muller, E. M., Mackin, N. A., Erdman, S. E., & Cunningham, K. W. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m304089200 · Cited by 112 · see citing works
Self-citation
Ca 2+ -Calmodulin Promotes Survival of Pheromone-Induced Growth Arrest by Activation of Calcineurin and Ca 2+ -Calmodulin-Dependent Protein Kinase
Moser, M. J., Geiser, J. R., & Davis, T. N. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.9.4824 · Cited by 104 · see citing works
New Regulators of a High Affinity Ca2+ Influx System Revealed through a Genome-wide Screen in Yeast
Martin, D. C., Kim, H., Mackin, N. A., Maldonado‐Báez, L., Evangelista, C. C., Beaudry, V. G., Dudgeon, D. D., Naiman, D. Q., Erdman, S. E., & Cunningham, K. W. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.177451 · Cited by 93 · see citing works
Self-citation
Alkaline stress triggers an immediate calcium fluctuation in Candida albicans mediated by Rim101p and Crz1p transcription factors
Wang, H., Liang, Y., Zhang, B., Zheng, W. L., Xing, L., & Li, M. (2011) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2011.00730.x · Cited by 85 · see citing works
Domain Architecture of the Regulators of Calcineurin (RCANs) and Identification of a Divergent RCAN in Yeast
Mehta, S., Li, H., Hogan, P. G., & Cunningham, K. W. (2009) · Molecular and Cellular Biology · doi:10.1128/mcb.01197-08 · Cited by 83 · see citing works
Self-citation
Regulatory Subunit (CNB1 Gene Product) of Yeast Ca2+/Calmodulin-Dependent Phosphoprotein Phosphatases Is Required for Adaptation to Pheromone
Cyert, M., & Thorner, J. W. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.8.3460-3469.1992 · Cited by 82 · see citing works
MID1, a Novel Saccharomyces cerevisiae Gene Encoding a Plasma Membrane Protein, Is Required for Ca 2+ Influx and Mating
Iida, H., Nakamura, H., Ono, T., Okumura, M. S., & Anraku, Y. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.12.8259-8271.1994 · Cited by 79 · see citing works
TRK1 and TRK2 Encode Structurally Related K + Transporters in Saccharomyces cerevisiae
Ko, C. H., & Gaber, R. F. (1991) · Molecular and Cellular Biology · doi:10.1128/mcb.11.8.4266-4273.1991 · Cited by 77 · see citing works
Structural correlates of selectivity and inactivation in potassium channels
McCoy, J. G., & Nimigean, C. M. (2011) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2011.09.007 · Cited by 76 · see citing works
Specific induction of Ca2+ transport activity in MATa cells of Saccharomyces cerevisiae by a mating pheromone, alpha factor.
Ohsumi, Y., & Anraku, Y. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)85109-8 · Cited by 75 · see citing works
Measurements of plasma membrane potential changes inSaccharomyces cerevisiaecells reveal the importance of the Tok1 channel in membrane potential maintenance
Marešová, L., Urbánková, E., Gášková, D., & Sychrová, H. (2006) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2006.00140.x · Cited by 68 · see citing works
NSC1: a novel high‐current inward rectifier for cations in the plasma membrane of Saccharomyces cerevisiae
Bihler, H. J., Slayman, C. L., & Bertl, A. (1998) · FEBS Letters · doi:10.1016/s0014-5793(98)00832-1 · Cited by 66 · see citing works
Self-citation
Low-affinity potassium uptake by Saccharomyces cerevisiae is mediated by NSC1, a calcium-blocked non-specific cation channel
Bihler, H. J., Slayman, C. L., & Bertl, A. (2002) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(01)00414-x · Cited by 64 · see citing works
Self-citation
The S. cerevisiae outwardly-rectifying potassium channel (DUK1) identifies a new family of channels with duplicated pore domains.
Reid, J. D., Lukas, W., Shafaatian, R., Bertl, A., Scheurmann-Kettner, C., Guy, H. R., & North, R. A. (1996) · PubMed · Cited by 55 · see citing works
Physiological Characterization of the Yeast Plasma Membrane Outward Rectifying K + Channel, DUK1 (TOK1), In Situ
Bertl, A., Bihler, H. J., Reid, J. D., Kettner, C., & Slayman, C. L. (1998) · The Journal of Membrane Biology · doi:10.1007/s002329900343 · Cited by 54 · see citing works
Self-citation
Cch1 Restores Intracellular Ca2+ in Fungal Cells during Endoplasmic Reticulum Stress
Hong, M.-P., Vu, K., Bautos, J., & Gelli, A. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.056218 · Cited by 54 · see citing works
Prm1 Prevents Contact-Dependent Lysis of Yeast Mating Pairs
Jin, H., Carlile, C., Nolan, S., & Grote, E. (2004) · Eukaryotic Cell · doi:10.1128/ec.3.6.1664-1673.2004 · Cited by 51 · see citing works
Estimation of the electric plasma membrane potential difference in yeast with fluorescent dyes: comparative study of methods
Peña, A., Sánchez, N. S., & Calahorra, M. (2010) · Journal of Bioenergetics and Biomembranes · doi:10.1007/s10863-010-9311-x · Cited by 35 · see citing works
Splitting the Two Pore Domains from TOK1 Results in Two Cationic Channels with Novel Functional Properties
Saldaña, C., Naranjo, D., Coria, R., Peña, A., & Vaca, L. A. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m107957200 · Cited by 11 · see citing works

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Zimmermannová, O., Felcmanová, K., Sácká, L., Colinet, A.-S., Morsomme, P., & Sychrová, H. (2021) · FEMS Yeast Research · doi:10.1093/femsyr/foab015 · Cited by 7 · see citing works
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Remines, M., Schoonover, M. G., Knox, Z., Kenwright, K., Hoffert, K. M., Coric, A., Mead, J., Ampfer, J., Seye, S., & Strome, E. D. (2024) · G3 Genes Genomes Genetics · doi:10.1093/g3journal/jkae002 · Cited by 6 · see citing works
Calmodulin Kinase 1 Regulates the Vacuolar Calcium Exporter Yvc1p in Saccharomyces cerevisiae
Kepler, J. L. (2014) · Fungal Genomics & Biology · doi:10.4172/2165-8056.1000114 · Cited by 6 · see citing works
Interactions Between Monovalent Cations and Nutrient Homeostasis
Canadell, D., & Ariño, J. (2015) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-25304-6_11 · Cited by 6 · see citing works
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Harrington, A. A., Nickels, T. J., & Cunningham, K. W. (2026) · mBio · doi:10.1128/mbio.02546-25 · Cited by 4 · see citing works
“An upstream activator of calcineurin ( KCH1 ) in S. cerevisiae and C. albicans (34, 35) was also present on this list, but was not grouped in this ontology.”
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Wang, D., Zhao, Z., Long, Y., & Fan, R. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241814266 · Cited by 4 · see citing works
Thermodynamic analysis of Kex2 activity: The acylation and deacylation steps are potassium- and substrate-dependent
Antunes, A. A., Jesus, L. D. O. P., Manfredi, M. A., de Souza, A. A., Machado, M. F. M., Silva, P. M. E., Icimoto, M. Y., Juliano, M. A., Juliano, L., & Júdice, W. A. D. S. (2017) · Biophysical Chemistry · doi:10.1016/j.bpc.2017.11.007 · Cited by 3 · see citing works

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Muller, E. M., Locke, E., & Cunningham, K. W. (2001) · Genetics · doi:10.1093/genetics/159.4.1527 · Cited by 133 · see citing works
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Yeast Kch1 and Kch2 membrane proteins play a pleiotropic role in membrane potential establishment and monovalent cation homeostasis regulation
Felcmanová, K., Nevečeřalová, P., Sychrová, H., & Zimmermannová, O. (2017) · FEMS Yeast Research · doi:10.1093/femsyr/fox053 · Cited by 7 · see citing works
The Effect of Potassium Ionophores and Potassium on Cellular Calcium in the Yeast Saccharomyces cerevisiae
Eilam, Y. (1982) · Microbiology · doi:10.1099/00221287-128-11-2611 · Cited by 13 · see citing works
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Martin, D. C., Kim, H., Mackin, N. A., Maldonado‐Báez, L., Evangelista, C. C., Beaudry, V. G., Dudgeon, D. D., Naiman, D. Q., Erdman, S. E., & Cunningham, K. W. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.177451 · Cited by 93 · see citing works
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Muller, E. M., Mackin, N. A., Erdman, S. E., & Cunningham, K. W. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m304089200 · Cited by 112 · see citing works
Self-citation

Authors: Stefan, C. P., Zhang, N., Sokabe, T., Rivetta, A., Slayman, C. L., Montell, C., & Cunningham, K. W.
Affiliations: Johns Hopkins University (US)
Yale University (US)
ORCID: Stefan, Christopher P.
Zhang, Nannan
Sokabe, Takaaki
Rivetta, Alberto
Slayman, Clifford L.
Montell, Craig
Cunningham, Kyle W.
Venue: Eukaryotic Cell
Publisher: American Society for Microbiology
Published: 2013-02
Volume: 12
Issue: 2
Pages: 204-214
ISSN: 1535-9778 (ISSN-L)
1535-9786
DOI: 10.1128/ec.00299-12
PMC: PMC3571297
OpenAlex: W2169212382
Semantic Scholar: 9edf9f23d2db11473ca7a81d84240ca920c8f483
Cited by: 32
References: 55
Citations by year: 2025: 1 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 1 · 2018: 3 · 2017: 3 · 2016: 7 · 2015: 7 · 2014: 2 · 2013: 2
FWCI: 1.41
Type: journal article
Language: en
Open access: diamond
doi.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Eukaryotic Cell (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
National Eye Institute (EY10852, R01EY010852)
National Institute of General Medical Sciences (R01GM053082, GM085335, GM 60696, R01GM085335, GM053082, R01GM060696)
National Institute of Neurological Disorders and Stroke (NS074072, R21NS074072)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
Keywords: potassium transporters, mating pheromone response, calcineurin, inward rectifier potassium current, plasma membrane proteins, Saccharomyces cerevisiae
MeSH terms: Mating Factor, Calcium Chloride, Humans, Hydrogen-Ion Concentration, Membrane Potentials, Peptides, Potassium, Saccharomyces cerevisiae, Calcium Channels, Gene Expression, Patch-Clamp Techniques, Calcium Signaling, Protein Transport, Cation Transport Proteins, Saccharomyces cerevisiae Proteins, Gene Knockout Techniques, HEK293 Cells

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Activation of an Essential Calcium Signaling Pathway in Saccharomyces cerevisiae by Kch1 and Kch2, Putative Low-Affinity Potassium Transporters

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