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Cited by 92Pneumonia and Respiratory InfectionsInfluenza Virus Research StudiesEar Surgery and Otitis Media

Effect of experimental influenza A virus infection on isolation of Streptococcus pneumoniae and other aerobic bacteria from the oropharynges of allergic and nonallergic adult subjects

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Wadowsky, R M, Mietzner, S M, Skoner, D P, Doyle, W J, Fireman, P·1995·Infection and Immunity
DOI 10.1128/iai.63.4.1153-1157.1995PMC PMC173127
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Abstract

Intranasal challenge with both influenza A virus and Streptococcus pneumoniae promotes otitis media with S. pneumoniae in chinchillas. We investigated whether influenza A virus infection promotes oropharyngeal colonization with S. pneumoniae and other middle ear pathogens by selectively inhibiting commensal bacteria. On study day 0, 12 allergic and 15 nonallergic adult subjects were intranasally inoculated with influenza A/Kawasaki (H1N1) virus. Every subject was infected with the virus as demonstrated by nasal shedding or seroconversion. Average upper respiratory symptom scores and nasal secretion weights from the entire subject group were elevated between days 2 and 6 (acute phase) and were not significantly different between allergic and nonallergic subjects. S. pneumoniae was not isolated from any subject prior to the virus challenge but was isolated in heavy density from 4 (15%) subjects on day 6 (P = 0.055). Staphylococcus aureus was isolated more frequently from the nonallergic subjects than from the allergic subjects on days 2 (80 versus 25%, respectively) 4, (67 versus 17%, respectively), and 6 (73 versus 25%, respectively) (P < 0.05). The isolation rates of other middle ear pathogens were not significantly different before virus challenge and during the acute and resolution phases (days 27 to 30) of the experimental infection for the entire subject group or either the allergic or nonallergic subgroup. Densities and isolation rates of commensal bacteria from the entire subject group were similar throughout the observational period. These results suggest that the virus infection promoted S. pneumoniae colonization of the oropharynx and that nonallergic persons may be more vulnerable to colonization with S. aureus than allergic persons. The altered colonization rates were not attributed to inhibition of commensal bacteria.

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Crossref · 10.1128/iai.63.4.1153-1157.1995

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  • In experimental in vitro models, viral infections increase the colonization rates of various bacteria in the URT, including S. pneumoniae and H. influenzae.

    “In experimental in vitro models, viral infections increase the colonization rates of various bacteria in the URT (90–95), including S. pneumoniae and H. influenzae (86–88).”

    From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [92]

  • In adult subjects inoculated with influenza, after 6 days 15% were heavily colonized by S. pneumoniae.

    “After 6 days 15% of the subjects were heavily colonized by S. pneumoniae (Wadowsky et al., 1995).”

    From Secondary bacterial infections associated with influenza pandemics (2017) · cites this paper as Wadowsky et al., 1995

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Authors
Wadowsky, R. M., Mietzner, S. M., Skoner, D. P., Doyle, W. J., & Fireman, P.
Affiliations
University of Pittsburgh (US)
ORCID
Venue
Infection and Immunity
Publisher
American Society for Microbiology
Published
1995-04
Volume
63
Issue
4
Pages
1153-1157
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
DOI
10.1128/iai.63.4.1153-1157.1995
PMC
PMC173127
OpenAlex
W2101621320
Cited by
92
References
34
Citations by year
2023: 1 · 2022: 3 · 2021: 5 · 2020: 1 · 2019: 3 · 2018: 3 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 11 · 2013: 4 · 2012: 4
FWCI
1.72
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Infection and Immunity (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Allergy and Infectious Diseases (R01AI019262, AI19262)
Topics
  • Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
  • Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
  • Health Sciences › Medicine › Otorhinolaryngology › Ear Surgery and Otitis Media
Keywords
influenza A virus, Streptococcus pneumoniae, Staphylococcus aureus, oropharyngeal colonization, commensal bacteria, otitis media, intranasal challenge, H1N1 influenza, upper respiratory symptoms, nasal shedding
MeSH terms
Acute Disease, Adult, Female, Humans, Hypersensitivity, Influenza, Human, Male, Middle Aged, Oropharynx, Influenza A virus, Pneumococcal Infections

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