Expression of the Candida albicans Gene ALS1 in Saccharomyces cerevisiae Induces Adherence to Endothelial and Epithelial Cells

Fu, Yue; Rieg, Günter; Fonzi, William A.; Belanger, Paul H.; Edwards, John E.; Filler, Scott G.

doi:10.1128/iai.66.4.1783-1786.1998

Cited by 159Antifungal resistance and susceptibilityFungal and yeast genetics researchFungal Infections and Studies

Expression of the Candida albicans Gene ALS1 in Saccharomyces cerevisiae Induces Adherence to Endothelial and Epithelial Cells

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Fu, Yue, Rieg, Günter, Fonzi, William A., Belanger, Paul H., Edwards, John E., Filler, Scott G.·1998·Infection and Immunity

DOI 10.1128/iai.66.4.1783-1786.1998PMC PMC108121

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Abstract

To identify genes encoding adhesins that mediate the binding of Candida albicans to endothelial cells, a genomic library from this organism was constructed and used to transform Saccharomyces cerevisiae . These transformed organisms were screened for adherence to endothelial cells, and a highly adherent clone was identified. The adherence of this clone to endothelial cells was over 100-fold greater than that of control S. cerevisiae transformed with the empty plasmid. This clone also exhibited enhanced adherence to epithelial cells. The C. albicans gene contained within this clone was found to be ALS1 . These results indicate that ALS1 may encode a candidal adhesin.

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Als1 is an adhesin.
“even though previous studies have clearly demonstrated that Als1 is an adhesin [36,37].”
From Als3 Is a Candida albicans Invasin That Binds to Cadherins and Induces Endocytosis by Host Cells (2007) · cites this paper as [37]
The abstract (p. 1) reports that expressing ALS1 in S. cerevisiae greatly increased adherence to endothelial cells and also enhanced epithelial-cell adherence. But it concludes only that ALS1 “may encode a candidal adhesin”; the discussion (p. 3) likewise calls this a supported hypothesis. The citing sentence overstates that qualified conclusion as a clear demonstration. (LLM, conf=0.88)
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“Recently, Gaur and Klotz (4) described another member of the ALS gene family, ALA1.”
Background
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Fu, Y., Filler, S., Spellberg, B., Fonzi, W., Ibrahim, A., Kanbe, T., Ghannoum, M., & Edwards, J. (1998) · Infection and Immunity · doi:10.1128/iai.66.5.2078-2084.1998 · Cited by 19 · see citing works
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[3]3. Fu, Y., S. G. Filler, B. J. Spellberg, W. A. Fonzi, A. S. Ibrahim, T. Kanbe, M. A. Ghannoum, and J. E. Edwards, Jr. Cloning and characterization of CAD1/AAF1 , a gene from Candida albicans that induces adherence to endothelium when expressed in Saccharomyces cerevisiae . Infect. Immun., in press.

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ARTICLES Identification of Candida albicans Genes that Induce Saccharomyces cerevisiae Cell Adhesion and
Li, F., & Palecek, S. P. (2015)

Authors: Fu, Y., Rieg, G., Fonzi, W. A., Belanger, P. H., Edwards, J. E., & Filler, S. G.
Affiliations: University of California, Los Angeles (US)
St. John’s Cardiovascular Research Center, Division of Infectious Diseases, Department of Medicine, Harbor-UCLA Research and Education Institute, Torrance, California 905021;
Georgetown University (US)
ORCID: Fu, Yue
Fonzi, William A.
Edwards, John E.
Filler, Scott G.
Venue: Infection and Immunity
Publisher: American Society for Microbiology
Published: 1998-04
Volume: 66
Issue: 4
Pages: 1783-1786
ISSN: 0019-9567 (ISSN-L)
1098-5522
DOI: 10.1128/iai.66.4.1783-1786.1998
PMC: PMC108121
OpenAlex: W1965039542
Cited by: 159
References: 12
Citations by year: 2025: 1 · 2023: 1 · 2022: 1 · 2021: 5 · 2020: 3 · 2019: 3 · 2018: 6 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 10 · 2013: 4 · 2012: 3
FWCI: 7.06
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Infection and Immunity (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
National Institute of Allergy and Infectious Diseases (P01 AI-37194, R29AI040636, R01AI019990, R01-AI19990, P01AI037194)
National Center for Research Resources (M01RR000425)
Topics: Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
Keywords: Candida albicans, adhesins, cell adhesion, endothelial cells, epithelial cells, yeast transformation, genomic library screening, fungal adhesion, host cell binding
MeSH terms: Adhesiveness, Candida albicans, Endothelium, Vascular, Epithelial Cells, Fungal Proteins, Genes, Fungal, Humans, Saccharomyces cerevisiae

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Expression of the Candida albicans Gene ALS1 in Saccharomyces cerevisiae Induces Adherence to Endothelial and Epithelial Cells

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