Glycan microarray analysis of Candida glabrata adhesin ligand specificity
Open accessAbstract
The Candida glabrata genome encodes at least 23 members of the EPA (epithelial adhesin) family responsible for mediating adherence to host cells. To better understand the mechanism by which the Epa proteins contribute to pathogenesis, we have used glycan microarray analysis to characterize their carbohydrate-binding specificities. Using Saccharomyces cerevisiae strains surface-expressing the N-terminal ligand-binding domain of the Epa proteins, we found that the three Epa family members functionally identified as adhesins in Candida glabrata (Epa1, Epa6 and Epa7) bind to ligands containing a terminal galactose residue. However, the specificity of the three proteins for glycans within this class varies, with Epa6 having a broader specificity range than Epa1 or Epa7. This result is intriguing given the close homology between Epa6 and Epa7, which are 92% identical at the amino acid level. We have mapped a five-amino-acid region within the N-terminal ligand-binding domain that accounts for the difference in specificity of Epa6 and Epa7 and show that these residues contribute to adherence to both epithelial and endothelial cell lines in vitro.
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Epa6 and Epa7 adhesins mediate adherence to epithelial and endothelial cells.
“The Epa6 and Epa7 adhesins have been shown to mediate adherence to epithelial and endothelial cells [30,35].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [35]
Epa1, Epa6 and Epa7 show glycan ligand specificity, with Epa6 having the widest substrate specificity.
“Despite the preference for the terminal galactose, Epa1, 6 and 7 showed glycan ligand specificity, with Epa6 having the widest substrate specificity [35,43].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [35]
Epa1, Epa6 and Epa7 bind to oligosaccharides containing a terminal galactose residue.
“With regard to ligands, Epa1, Epa6 and Epa7 were found to bind to oligosaccharides containing a terminal galactose residue [35].”
From Candida glabrata: A Lot More Than Meets the Eye (2019) · cites this paper as [35]
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- Authors
- Zupancic, M. L., Frieman, M., Smith, D., Alvarez, R. A., Cummings, R. D., & Cormack, B. P.
- Affiliations
- Johns Hopkins University (US)
- University of North Carolina at Chapel Hill (US)
- Emory University (US)
- University of Oklahoma Health Sciences Center (US)
- Corresponding author
- Cormack, Brendan P.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2008-03-25
- Volume
- 68
- Issue
- 3
- Pages
- 547-559
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2102496935
- Cited by
- 143
- References
- 43
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 3 · 2023: 7 · 2022: 2 · 2021: 11 · 2020: 6 · 2019: 6 · 2018: 8 · 2017: 6 · 2016: 7 · 2015: 6 · 2014: 11 · 2013: 14 · 2012: 8
- FWCI
- 8.62
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM 62116, U54GM062116)
- National Institute of Allergy and Infectious Diseases (R01AI046223)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK075732, DK075732, DK 49720, P01DK049720)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Keywords
- Candida glabrata, carbohydrate binding specificity, galactose, ligand-binding domain, fungal adhesion, host cell adhesion, endothelial cell adhesion, glycan microarrays
- MeSH terms
- Amino Acid Sequence, Animals, Cell Adhesion, Cell Line, Fungal Proteins, Guinea Pigs, Ligands, Molecular Sequence Data, Polysaccharides, Protein Binding, Saccharomyces cerevisiae, Structure-Activity Relationship, Cell Adhesion Molecules, Sequence Alignment, Sequence Homology, Amino Acid, Protein Structure, Tertiary, Amino Acid Motifs, Protein Interaction Mapping, Protein Array Analysis, Candida glabrata, Endothelial Cells, Host-Pathogen Interactions
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