Cited by 177Metalloenzymes and iron-sulfur proteinsMicrobial metabolism and enzyme functionMicrobial Fuel Cells and Bioremediation

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Electron Bifurcation Involved in the Energy Metabolism of the Acetogenic Bacterium Moorella thermoacetica Growing on Glucose or H 2 plus CO 2

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Huang, Haiyan, Wang, Shuning, Moll, Johanna, Thauer, Rudolf K.·2012·Journal of Bacteriology

DOI 10.1128/jb.00385-12PMC PMC3393501

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Abstract

Moorella thermoacetica ferments glucose to three acetic acids. In the oxidative part of the fermentation, the hexose is converted to 2 acetic acids and 2 CO 2 molecules with the formation of 2 NADH and 2 reduced ferredoxin (Fd red 2− ) molecules. In the reductive part, 2 CO 2 molecules are reduced to acetic acid, consuming the 8 reducing equivalents generated in the oxidative part. An open question is how the two parts are electronically connected, since two of the four oxidoreductases involved in acetogenesis from CO 2 are NADP specific rather than NAD specific. We report here that the 2 NADPH molecules required for CO 2 reduction to acetic acid are generated by the reduction of 2 NADP + molecules with 1 NADH and 1 Fd red 2− catalyzed by the electron-bifurcating NADH-dependent reduced ferredoxin:NADP + oxidoreductase (NfnAB). The cytoplasmic iron-sulfur flavoprotein was heterologously produced in Escherichia coli , purified, and characterized. The purified enzyme was composed of 30-kDa (NfnA) and 50-kDa (NfnB) subunits in a 1-to-1 stoichiometry. NfnA harbors a [2Fe2S] cluster and flavin adenine dinucleotide (FAD), and NfnB harbors two [4Fe4S] clusters and FAD. M. thermoacetica contains a second electron-bifurcating enzyme. Cell extracts catalyzed the coupled reduction of NAD + and Fd with 2 H 2 molecules. The specific activity of this cytoplasmic enzyme was 3-fold higher in H 2 -CO 2 -grown cells than in glucose-grown cells. The function of this electron-bifurcating hydrogenase is not yet clear, since H 2 -CO 2 -grown cells additionally contain high specific activities of an NADP + -dependent hydrogenase that catalyzes the reduction of NADP + with H 2 . This activity is hardly detectable in glucose-grown cells.

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Authors: Huang, H., Wang, S., Moll, J., & Thauer, R. K.
Affiliations: Max Planck Institute for Terrestrial Microbiology (DE)
Shandong University (CN)
ORCID: Huang, Haiyan
Wang, Shuning
Thauer, Rudolf K.
Venue: Journal of Bacteriology
Publisher: American Society for Microbiology
Published: 2012-07-15
Volume: 194
Issue: 14
Pages: 3689-3699
ISSN: 0021-9193 (ISSN-L)
1098-5530
DOI: 10.1128/jb.00385-12
PMC: PMC3393501
OpenAlex: W2117142868
Cited by: 177
References: 74
Citations by year: 2026: 12 · 2025: 6 · 2024: 12 · 2023: 9 · 2022: 8 · 2021: 13 · 2020: 6 · 2019: 13 · 2018: 15 · 2017: 15 · 2016: 16 · 2015: 18 · 2014: 15 · 2013: 12 · 2012: 7
FWCI: 6.38
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Journal of Bacteriology (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Max Planck Institute for Plasma Physics (submittedVersion)
MPG.PuRe (Max Planck Society) (submittedVersion)
Funders: Max-Planck-Gesellschaft
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Metalloenzymes and iron-sulfur proteins
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
Keywords: electron bifurcation, Moorella thermoacetica, acetogenesis, hydrogenase, NADPH production, ferredoxin, iron-sulfur flavoprotein, glucose fermentation, energy metabolism, NADH oxidation
MeSH terms: Carbon Dioxide, Electrons, Energy Metabolism, Glucose, Hydrogen, Molecular Sequence Data, Oxidoreductases, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Enzymologic, Moorella

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Electron Bifurcation Involved in the Energy Metabolism of the Acetogenic Bacterium Moorella thermoacetica Growing on Glucose or H 2 plus CO 2

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