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A SELECTIVE MEDIUM FOR THE ISOLATION AND ENUMERATION OF ORAL AND FECAL LACTOBACILLI
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“Where high populations of lactococci are expected in conjunction with lacticaseibacilli; such as is the case with cheese LBS is the medium of choice.”
From Protection of candidate probiotic lactobacilli by Cheddar cheese matrix during simulated gastrointestinal digestion (2022) · cites this paper as Rogosa et al., 1951
In the citing paper
“MRS and LBS, based on the work of Rogosa et al (1951) are designed to support the growth of lacticaseibacilli.The key difference between both media is that LBS contains ammonium citrate, sodium acetate, acetic acid and ferrous sulphate that act as inhibitors of lactococci and provide a low pH environment which is tolerated by lactobacilli.”
From Protection of candidate probiotic lactobacilli by Cheddar cheese matrix during simulated gastrointestinal digestion (2022) · cites this paper as Rogosa et al., 1951
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“Rogosa broth (Rogosa): tryptone 10 g, yeast extract 5 g, glucose 10 g, arabinose 5 g, sucrose 5 g, KH2PO4 6 g, ammonium citrate 2 g, sodium acetate 15 g, Tween80 1 ml, MgSO4 0.57 g, MgSO4 0.12 g, FeSO4 0.03 g, distilled water 1000 ml, pH 5.3–5.5 (Rogosa et al., 1951).”
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“A high level was considered to be >105 cfu/mL for MS, >104 cfu/mL for LB and ≥102 cfu/ mL for yeast (27,28,29).”
BackgroundThe Effects of Irrigation, Genotype and Additives on Tef Silage Making
Wagali, P., Sabastian, C., Saranga, Y., Ben‐Zeev, S., & Mabjeesh, S. J. (2023) · Animals · doi:10.3390/ani13030470 · Cited by 6
“A portion of the filtrate was used for microbial analysis; enumeration of LAB was performed using Rogosa SL Agar (Himedia®, Mumbai, India) according to [35], and enumeration of moulds and yeast was performed using Malt Extract Agar (Sigma_Aldrich®, Rehovot, Israel).”
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BackgroundMethodRecovery of Lactobacillus bulgaricus and Streptococcus thermophilus on Nine Commonly Used Agar Media
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“2%) and Group II: included (20) children who rinsed with Sodium Hexametaphosphate (7%).”
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“Microbiological counts (log10UFC g−1) were carried out in duplicates, of different DOI: 10.4236/fns.2018.912101 1395 Food and Nutrition Sciences dilutions, pour plating in selective media: Rogosa agar medium (Difco) for Lactobacillus [32], modified MRS agar (Difco) [33] for Bifidobacterium, RCM agar (Difco) for Clostr…”
MethodInfluentialISOLATION OF ANTIBIOTIC-RESISTANT LACTOBACILLI
Ellis, R. H., & Sarles, W. B. (1958) · Journal of Bacteriology · doi:10.1128/jb.75.3.272-277.1958 · Cited by 3
Wagali, P., Pelech, I., Sabastian, C., Ari, J. B., Tagari, H., & Mabjeesh, S. J. (2023) · Animals · doi:10.3390/ani13132197 · Cited by 5
“A portion of the filtrate was used for microbial analysis, enumeration of LAB using Rogosa SL Agar (Himedia, Mumbai, India) according to [16], enumeration of molds and yeast using Malt Extract Agar (Sigma-Aldrich, Rehovot, Israel) according to the detailed method described by [17].”
MethodStudies on the Amino Acid Decarboxylases of Oral Lactobacilli
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A Comparison of the Snyder Colorimetric Test with the Lactobacillus Count Using Rogosa Medium
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The microbial flora of Awshari cheese with special reference to lactobacilli
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Section B. Bacteriology and mycology applied to dairying
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LACTOBACILLUS | Lactobacillus Acidophilus
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EBC ANALYTICA MICROBIOLOGICA: PART II CONTINUED 2. ANALYTICAL METHODS
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“Lactic acid bacteriawere enumerated in two different culture media: M17 agar (Merck), a selective medium for lactococci [22], after incubation at 30 1°C for 18–24 h; Man–Rogosa–Sharpe (MRS)-agar (Oxoid), a selective medium for lactobacilli [23], after incubation anaerobically at 30 1°C for 72 h.”
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“) after incubation at 30°C for 5 days (Rogosa et al., 1951).”
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“- Group II: included (20) children who rinsed with Sodium Hexametaphosphate (7%).”
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Effect of Calcium, Phosphorus and Manganese on the Intestinal Synthesis of Thiamine in Rats
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Metadata
- Authors
- Rogosa, M., Mitchell, J. A., & Wiseman, R. F.
- Affiliations
- National Institutes of Health (US)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1951-07
- Volume
- 62
- Issue
- 1
- Pages
- 132-133
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC386093
- OpenAlex
- W1589430658
- Cited by
- 653
- References
- 0
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 5 · 2023: 9 · 2022: 5 · 2021: 4 · 2020: 10 · 2019: 5 · 2018: 9 · 2017: 15 · 2016: 10 · 2015: 20 · 2014: 13 · 2013: 7 · 2012: 10
- FWCI
- 26.74
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- lactobacilli, selective culture media, bacterial isolation, oral bacteria, fecal bacteria, lactic acid bacteria
- MeSH terms
- Feces, Humans, Lactobacillus
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