Set of Classical PCRs for Detection of Mutations in Candida glabrata FKS Genes Linked with Echinocandin Resistance
Open accessAbstract
Clinical echinocandin resistance among Candida glabrata strains is increasing, especially in the United States. Antifungal susceptibility testing is considered mandatory to guide therapeutic decisions. However, these methodologies are not routinely performed in the hospital setting due to their complexity and the time needed to obtain reliable results. Echinocandin failure in C. glabrata is linked exclusively to Fks1p and Fks2p amino acid substitutions, and detection of such substitutions would serve as a surrogate marker to identify resistant isolates. In this work, we report an inexpensive, simple, and quick classical PCR set able to objectively detect the most common mechanisms of echinocandin resistance in C. glabrata within 4 h. The usefulness of this assay was assessed using a blind collection of 50 C. glabrata strains, including 16 FKS1 and/or FKS2 mutants.
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- Authors
- Dudiuk, C., Gamarra, S., Leonardeli, F., Jimenez-Ortigosa, C., Vitale, R. G., Afeltra, J., Perlin, D. S., & Garcia-Effron, G.
- Affiliations
- Consejo Nacional de Investigaciones Científicas y Técnicas (AR)
- Universidad Nacional del Litoral (AR)
- Public Health Research Institute, Rutgers, The State University of New Jersey, Newark, New Jersey, USA
- Rutgers, The State University of New Jersey (US)
- Laboratorio de Micología y Diagnóstico Molecular, Cátedra de Parasitología y Micología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina
- Venue
- Journal of Clinical Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2014-07
- Volume
- 52
- Issue
- 7
- Pages
- 2609-2614
- ISSN
- 0095-1137 (ISSN-L)
- 1098-660X
- PMC
- PMC4097693
- OpenAlex
- W1984516995
- Cited by
- 50
- References
- 39
- Citations by year
- 2026: 1 · 2025: 3 · 2023: 3 · 2022: 3 · 2021: 11 · 2020: 4 · 2019: 6 · 2018: 3 · 2017: 8 · 2016: 1 · 2015: 4 · 2014: 3
- FWCI
- 3.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Americanae (AECID Library) (submittedVersion, cc-by-nc)
- Conicet (submittedVersion, cc-by-nc-sa)
- Funders
- Pfizer
- Consejo Nacional de Investigaciones Científicas y Técnicas
- Universidad Nacional del Litoral
- National Institute of Allergy and Infectious Diseases (AI069397, R01AI069397)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fungal Plant Pathogen Control
- Keywords
- Candida glabrata, echinocandin resistance, FKS1, FKS2, amino acid substitutions, antifungal susceptibility testing, surrogate markers
- MeSH terms
- Antifungal Agents, Fungal Proteins, Glucosyltransferases, Humans, Microbial Sensitivity Tests, United States, Polymerase Chain Reaction, Amino Acid Substitution, Mutation, Missense, Drug Resistance, Fungal, Molecular Diagnostic Techniques, Candida glabrata, Mutant Proteins, Echinocandins
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