Cited by 147Virus-based gene therapy researchParvovirus B19 Infection StudiesVirology and Viral Diseases

The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.

Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins

Open access

Wistuba, A, Weger, S, Kern, A, Kleinschmidt, J A·1995·Journal of Virology

DOI 10.1128/jvi.69.9.5311-5319.1995PMC PMC189369

Open this paper in CoChat — free account

Abstract

The proteins encoded by the adeno-associated virus type 2 (AAV-2) rep and cap genes obtained during a productive infection of HeLa cells with AAV-2 and adenovirus type 2 were fractionated according to solubility, cellular localization, and sedimentation properties. The majority of Rep and Cap proteins accumulated in the nucleus, where they distributed into a soluble and an insoluble fraction. Analysis of the soluble nuclear fraction of capsid proteins by sucrose density gradients showed that they formed at least three steady-state pools: a monomer pool sedimenting at about 6S, a pool of oligomeric intermediates sedimenting between 10 and 15S, and a broad pool of assembly products with a peak between 60 and 110S, the known sedimentation positions of empty and full capsids. While the soluble nuclear monomer and oligomer pool contained predominantly only two capsid proteins, the 30 to 180S assembly products contained VP1, VP2, and VP3 in a stoichiometry similar to that of purified virions. They probably represent different intermediates in capsid assembly, DNA encapsidation, and capsid maturation. In contrast, the cytoplasmic fraction of capsid proteins showed a pattern of oligomers continuously increasing in size without a defined peak, suggesting that assembly of 60S particles occurs in the nucleus. Soluble nuclear Rep proteins were distributed over the whole sedimentation range, probably as a result of association with AAV DNA. Subfractions of the Rep proteins with defined sedimentation values were obtained in the soluble nuclear and cytoplasmic fractions. We were able to coimmunoprecipitate capsid proteins sedimenting between 60 and 110S with antibodies against Rep proteins, suggesting that they exist in common complexes possibly involved in AAV DNA packaging. Antibodies against the capsid proteins, however, precipitated Rep78 and Rep68 predominantly with a peak around 30S representing a second complex containing Rep and Cap proteins.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1128/jvi.69.9.5311-5319.1995

Cites

44 of 44 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Antibodies, a laboratory manual
(1989) · Biomedicine & Pharmacotherapy · doi:10.1016/0753-3322(89)90130-3 · Cited by 12,318
[6] Use of T7 RNA polymerase to direct expression of cloned genes
Studier, F. W., Rosenberg, A. H., Dunn, J. J., & Dubendorff, J. W. (1990) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(90)85008-c · Cited by 6,562
Adenovirus-Associated Defective Virus Particles
Atchison, R. W., Casto, B. C., & Hammon, W. M. (1965) · Science · doi:10.1126/science.149.3685.754 · Cited by 1,004
Site-specific integration by adeno-associated virus.
Kotin, R. M., Siniscalco, M., Samulski, R. J., Zhu, X. D., Hunter, L., Laughlin, C. A., McLaughlin, S., Muzyczka, N., Rocchi, M., & Berns, K. I. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.6.2211 · Cited by 906
Site-specific integration by adeno-associated virus.
Linden, R. M., Ward, P., Giraud, C., Winocour, E., & Berns, K. I. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.21.11288 · Cited by 805
Targeted integration of adeno‐associated virus (AAV) into human chromosome 19.
Samulski, R. J., Zhu, X., Xiao, X., Brook, J. D., Housman, D. E., Epstein, N., & Hunter, L. A. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb04964.x · Cited by 783
An octamer of histones in chromatin and free in solution.
Thomas, J. O., & Kornberg, R. D. (1975) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.72.7.2626 · Cited by 723
Nucleotide sequence and organization of the adeno-associated virus 2 genome
Srivastava, A., Lusby, E. W., & Berns, K. I. (1983) · Journal of Virology · doi:10.1128/jvi.45.2.555-564.1983 · Cited by 721
The AAV origin binding protein Rep68 is an ATP-dependent site-specific endonuclease with DNA helicase activity
Im, D., & Muzyczka, N. (1990) · Cell · doi:10.1016/0092-8674(90)90526-k · Cited by 470
Parvovirus replication
Berns, K. I. (1990) · Microbiological Reviews · doi:10.1128/mr.54.3.316-329.1990 · Cited by 363

Show all 44 references

Sorted by citations

Novel Tools for Production and Purification of Recombinant Adenoassociated Virus Vectors
Grimm, D., Kern, A., Rittner, K., & Kleinschmidt, J. A. (1998) · Human Gene Therapy · doi:10.1089/hum.1998.9.18-2745 · Cited by 701
Self-citation
In Vitro and In Vivo Gene Therapy Vector Evolution via Multispecies Interbreeding and Retargeting of Adeno-Associated Viruses
Grimm, D., Lee, J. S., Wang, L., Desai, T., Akache, B., Storm, T. A., & Kay, M. A. (2008) · Journal of Virology · doi:10.1128/jvi.00254-08 · Cited by 696
Mutational Analysis of the Adeno-Associated Virus Type 2 (AAV2) Capsid Gene and Construction of AAV2 Vectors with Altered Tropism
Wu, P., Xiao, W. S., Conlon, T. J., Hughes, J. A., Agbandje‐McKenna, M., Ferkol, T. W., Flotte, T. R., & Muzyczka, N. (2000) · Journal of Virology · doi:10.1128/jvi.74.18.8635-8647.2000 · Cited by 396
“The A20 antibody detects both full and empty particles (44).”
Background
A viral assembly factor promotes AAV2 capsid formation in the nucleolus
Sonntag, F., Schmidt, K., & Kleinschmidt, J. A. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1001673107 · Cited by 392
Self-citation
Identification of a heparin-binding motif on adeno-associated virus type 2 capsids
Kern, A., Schmidt, K., Leder, C., Müller, O. J., Wobus, C. E., Bettinger, K., Von der Lieth, C. W., King, J. A., & Kleinschmidt, J. A. (2003) · Journal of Virology · doi:10.1128/jvi.77.20.11072-11081.2003 · Cited by 380
Self-citation
Identification of amino acid residues in the capsid proteins of adeno-associated virus type 2 that contribute to heparan sulfate proteoglycan binding
Opie, S. R., Warrington, Agbandje-McKenna, M., Zolotukhin, S., & Muzyczka, N. (2003) · Journal of Virology · doi:10.1128/jvi.77.12.6995-7006.2003 · Cited by 364
“bled particles, both full and empty (49).”
Helper virus-free, optically controllable, and two-plasmid-based production of adeno-associated virus vectors of serotypes 1 to 6
Grimm, D., Kay, M. A., & Kleinschmidt, J. A. (2003) · Molecular Therapy · doi:10.1016/s1525-0016(03)00095-9 · Cited by 344
“For detection of AAV VP proteins, we used the monoclonal antibody B1 [19], which binds to an 8-amino-acid-long epitope conserved among all AAV serotypes (Fig.”
BackgroundMethodInfluentialSelf-citation
HIV-1 in semen: Determination of proviral and viral titres compared to blood, and quantification of semen leukocyte populations
Ball, J. K., Curran, R., Irving, W. L., & Dearden, A. A. (1999) · Journal of Medical Virology · doi:10.1002/(sici)1096-9071(199911)59:3 · Cited by 34
The VP1 capsid protein of adeno-associated virus type 2 is carrying a phospholipase A2 domain required for virus infectivity
Girod, A., Wobus, C. E., Zádori, Z., Ried, M., Leike, K., Tijssen, P., Kleinschmidt, J. A., & Hallek, M. (2002) · Journal of General Virology · doi:10.1099/0022-1317-83-5-973 · Cited by 354
“The next detectable event during a productive AAV-2 infection is early gene expression (Trempe et al., 1987 ; Redemann et al., 1989 ; Wistuba et al., 1995).”
BackgroundSelf-citation
DNA helicase‐mediated packaging of adeno‐associated virus type 2 genomes into preformed capsids
King, J. A., Dubielzig, R., Grimm, D., & Kleinschmidt, J. A. (2001) · The EMBO Journal · doi:10.1093/emboj/20.12.3282 · Cited by 338
Self-citation
Adeno-Associated Virus Type 2 Capsids with Externalized VP1/VP2 Trafficking Domains Are Generated prior to Passage through the Cytoplasm and Are Maintained until Uncoating Occurs in the Nucleus
Sonntag, F., Bleker, S., Leuchs, B., Fischer, R., & Kleinschmidt, J. A. (2006) · Journal of Virology · doi:10.1128/jvi.01056-06 · Cited by 297
“proteins, respectively, were used as described previously (70).”
BackgroundMethodSelf-citation
Monoclonal Antibodies against the Adeno-Associated Virus Type 2 (AAV-2) Capsid: Epitope Mapping and Identification of Capsid Domains Involved in AAV-2–Cell Interaction and Neutralization of AAV-2 Infection
Wobus, C. E., Hügle-Dörr, B., Girod, A., Petersen, G., Hallek, M., & Kleinschmidt, J. A. (2000) · Journal of Virology · doi:10.1128/jvi.74.19.9281-9293.2000 · Cited by 273
“VP1, VP2, and VP3 assemble in the nucleus (52, 53) into mature virions in a 1:1:20 stoichiometry (33).”
BackgroundSelf-citation
Epitope Mapping of Human Anti-Adeno-Associated Virus Type 2 Neutralizing Antibodies: Implications for Gene Therapy and Virus Structure
Moskalenko, M., Chen, L., van Roey, M., Donahue, B. A., Snyder, R. O., McArthur, J. G., & Patel, S. D. (2000) · Journal of Virology · doi:10.1128/jvi.74.4.1761-1766.2000 · Cited by 253
“This same pattern was observed with five serum samples (Ser3, Ser6, Ser7, Ser23, and Ser24) and also with a neutralizing anti-AAV mouse monoclonal antibody, A20 (28, 29).”
Background
Targeted adeno-associated virus vector transduction of nonpermissive cells mediated by a bispecific F(ab'γ)2 antibody
Bartlett, J. S., Kleinschmidt, J. A., Boucher, R. C., & Samulski, R. J. (1999) · Nature Biotechnology · doi:10.1038/6185 · Cited by 229
“Attachment is achieved by means of a bispecific (bs) F(ab´g) 2 antibody in which one Fab´g arm recognizes cell–surface a IIb b 3 and the other recognizes the AAV capsid structur”
BackgroundSelf-citation
Adeno-associated virus capsid assembly is divergent and stochastic
Wörner, T. P., Bennett, A. D., Habka, S., Snijder, J., Friese, O. V., Powers, T. W., Agbandje‐McKenna, M., & Heck, A. J. R. (2021) · Nature Communications · doi:10.1038/s41467-021-21935-5 · Cited by 228
AAV Capsid Structure and Cell Interactions
Agbandje-McKenna, M., & Kleinschmidt, J. (2012) · Methods in Molecular Biology · doi:10.1007/978-1-61779-370-7_3 · Cited by 215
Self-citation
Structurally mapping the diverse phenotype of adeno-associated virus serotype 4
Govindasamy, L., Padron, E., McKenna, R., Muzyczka, N., Kaludov, N., Chiorini, J. A., & Agbandje-McKenna, M. (2006) · Journal of Virology · doi:10.1128/jvi.01536-06 · Cited by 205
“Peptide mapping has been used to identify both linear and conformationdependent epitopes on the AAV2 capsid (66, 67).”
Background
Adeno-Associated Virus Type 2 VP2 Capsid Protein Is Nonessential and Can Tolerate Large Peptide Insertions at Its N Terminus
Warrington, K. H., Gorbatyuk, O. S., Harrison, J. K., Opie, S. R., Zolotukhin, S., & Muzyczka, N. (2004) · Journal of Virology · doi:10.1128/jvi.78.12.6595-6609.2004 · Cited by 205
“mulate during AAV infection (9, 21, 25, 37, 57, 58) indicate”
Background
Mutational analysis of narrow pores at the fivefold symmetry axes of adeno-associated virus type 2 capsids reveals a dual role in genome packaging and activation of phospholipase A2 activity
Bleker, S., Sonntag, F., & Kleinschmidt, J. A. (2005) · Journal of Virology · doi:10.1128/jvi.79.4.2528-2540.2005 · Cited by 200
“mixed with an equal volume of 2 protein sample buffer (76) boiled immedi-”
BackgroundMethodInfluentialSelf-citation
Subcellular compartmentalization of adeno-associated virus type 2 assembly
Wistuba, A., Kern, A., Weger, S., Grimm, D., & Kleinschmidt, J. A. (1997) · Journal of Virology · doi:10.1128/jvi.71.2.1341-1352.1997 · Cited by 219
“The transcripts from both promoters are translated from spliced and unspliced mRNAs, resulting in four proteins designated Rep78, Rep68, Rep52 and Rep40 according to their apparent molecular weight.”
BackgroundMethodResultInfluentialSelf-citation
AAV hybrid serotypes: improved vectors for gene delivery
Choi, V., McCarty, D., & Samulski, R. (2005) · Current Gene Therapy · doi:10.2174/1566523054064968 · Cited by 191
Production of Human Papillomavirus Type 16 Virus-Like Particles in Transgenic Plants
Sonnewald, S., Sonnewald, U., Galmbacher, P., Willmitzer, L., & Müller, M. (2003) · Journal of Virology · doi:10.1128/jvi.77.17.9211-9220.2003 · Cited by 190
“As a 60 S sedimentation marker, empty VLPs of adeno-associated virus were used (45).”
Method
The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes
Sonntag, F., Köther, K., Schmidt, K., Weghofer, M., Raupp, C., Nieto, K., Kuck, A., Gerlach, B., Böttcher, B., Müller, O. J., et al. (2011) · Journal of Virology · doi:10.1128/jvi.05359-11 · Cited by 184
Self-citation
Separate basic region motifs within the adeno-associated virus capsid proteins are essential for infectivity and assembly
Grieger, J. C., Snowdy, S., & Samulski, R. J. (2006) · Journal of Virology · doi:10.1128/jvi.02723-05 · Cited by 172
“Previous studies have established that AAV2 assembles in the nucleus in “intranuclear assembly centers” that increase in size over time as more capsid proteins enter and virions are assembled (23, 59, 60).”
BackgroundResult
Insertional Mutagenesis of the Adeno-Associated Virus Type 2 (AAV2) Capsid Gene and Generation of AAV2 Vectors Targeted to Alternative Cell-Surface Receptors
Shi, W., Arnold, G. S., & Bartlett, J. S. (2001) · Human Gene Therapy · doi:10.1089/104303401750476212 · Cited by 167
“The A20 monoclonal antibody detects both full and empty AAV particles (Wistuba et al ., 1995).”
Enhancement of Adeno-Associated Virus Infection by Mobilizing Capsids into and Out of the Nucleolus
Johnson, J. S., & Samulski, R. J. (2008) · Journal of Virology · doi:10.1128/jvi.02309-08 · Cited by 167
“During replication, AAV monomers and subunits can be readily detected in cytoplasm and nuclei by antibody B1 (64, 65), but in our studies and others, researches have failed to detect significant B1 staining during infection (references 38 and 57 and data not shown).”
Result
OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1–12 Vectors for Gene Therapy
Mietzsch, M., Grasse, S., Zurawski, C., Weger, S., Bennett, A. D., Agbandje‐McKenna, M., Muzyczka, N., Zolotukhin, S., & Heilbronn, R. (2013) · Human Gene Therapy · doi:10.1089/hum.2013.184 · Cited by 141
“Western blot analysis was performed as described (Winter et al., 2012) using 1:10 dilutions of hybridoma supernatants monoclonal antibody (mAb) B1 (anti-VP1–3), mAb A1 (anti-VP1), or mAb 303.9 antibody (anti-Rep) (Wistuba et al., 1995, 1997).”
BackgroundMethodInfluentialSelf-citation
Mutational analysis of adeno-associated virus Rep protein-mediated inhibition of heterologous and homologous promoters
Hörer, M., Weger, S., Butz, K., Hoppe‐Seyler, F., Geisen, C., & Kleinschmidt, J. A. (1995) · Journal of Virology · doi:10.1128/jvi.69.9.5485-5496.1995 · Cited by 135
“Monoclonal Rep antibody 303/9 (67) as well as polyclonal Rep antiserum 90 (52) have been described elsewhere.”
BackgroundSelf-citation
Mapping the AAV Capsid Host Antibody Response toward the Development of Second Generation Gene Delivery Vectors
Tseng, Y.-S., & Agbandje‐McKenna, M. (2014) · Frontiers in Immunology · doi:10.3389/fimmu.2014.00009 · Cited by 143
“(55) also used a peptide mapping strategy to determine the epitopes of three mouse MAbs, A1, A69, and B1 previously reported to react against AAV2 (72, 73).”
Method
HOW DO ANIMAL DNA VIRUSES GET TO THE NUCLEUS?
Kasamatsu, H., & Nakanishi, A. (1998) · Annual Review of Microbiology · doi:10.1146/annurev.micro.52.1.627 · Cited by 130
Endocytosis of Adeno-Associated Virus Type 5 Leads to Accumulation of Virus Particles in the Golgi Compartment
Bantel‐Schaal, U., Hub, B., & Kartenbeck, J. (2002) · Journal of Virology · doi:10.1128/jvi.76.5.2340-2349.2002 · Cited by 130
“Mouse monoclonal antibody B1 (64) (a kind gift from”
Background
Human Adeno-Associated Virus Type 5 Is Only Distantly Related to Other Known Primate Helper-Dependent Parvoviruses
Bantel-Schaal, U., Delius, H., Schmidt, R., & zur Hausen, H. (1999) · Journal of Virology · doi:10.1128/jvi.73.2.939-947.1999 · Cited by 128
Receptor targeting of adeno-associated virus vectors
Büning, H., Ried, M. U., Perabò, L., Gerner, F., Huttner, N. A., Enssle, J., & Hallek, M. J. (2003) · Gene Therapy · doi:10.1038/sj.gt.3301976 · Cited by 124
Genetic modifications of the adeno-associated virus type 2 capsid reduce the affinity and the neutralizing effects of human serum antibodies
Huttner, N. A., Girod, A., Perabo, L., Edbauer, D., Kleinschmidt, J. A., Büning, H., & Hallek, M. (2003) · Gene Therapy · doi:10.1038/sj.gt.3302123 · Cited by 124
Self-citation
Building a Better Vector: The Manipulation of AAV Virions
Rabinowitz, J. E., & Samulski, R. J. (2000) · Virology · doi:10.1006/viro.2000.0707 · Cited by 118
Heparin binding induces conformational changes in Adeno-associated virus serotype 2
Levy, H. C., Bowman, V. D., Govindasamy, L., McKenna, R., Nash, K., Warrington, K., Chen, W., Muzyczka, N., Yan, X., Baker, T. S., et al. (2009) · Journal of Structural Biology · doi:10.1016/j.jsb.2008.12.002 · Cited by 110
“…Rep-facilitated DNA packaging is proposed to occur with preformed empty capsids through the channel, these observations suggest that small portions of viral genome are pre-packaged during assembly before Rep completes the packaging (Myers and Carter, 1980; Wistuba et al., 1995; King et al., 2001).”
Background
Impact of VP1-Specific Protein Sequence Motifs on Adeno-Associated Virus Type 2 Intracellular Trafficking and Nuclear Entry
Popa-Wagner, R., Porwal, M., Kann, M., Reuss, M., Weimer, M., Florin, L., & Kleinschmidt, J. A. (2012) · Journal of Virology · doi:10.1128/jvi.00282-12 · Cited by 109
“3B, A20 signal).”
MethodInfluentialSelf-citation
Enhancing the Clinical Potential of AAV Vectors by Capsid Engineering to Evade Pre-Existing Immunity
Bartel, M. (2011) · Frontiers in Microbiology · doi:10.3389/fmicb.2011.00204 · Cited by 108
Nanobody-Enhanced Targeting of AAV Gene Therapy Vectors
Eichhoff, A. M., Börner, K., Albrecht, B., Schäfer, W., Baum, N., Haag, F., Körbelin, J., Trepel, M., Braren, I., Grimm, D., et al. (2019) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2019.09.003 · Cited by 118
“Bound AAV particles were detected with a fluorochrome-conjugated heavy chain antibody, derived from AAV2-specific mAb A20.”
BackgroundMethod
Adeno-Associated Virus Type 2 Protein Interactions: Formation of Pre-Encapsidation Complexes
Dubielzig, R., King, J., Weger, S., Kern, A., & Kleinschmidt, J. A. (1999) · Journal of Virology · doi:10.1128/jvi.73.11.8989-8998.1999 · Cited by 105
“Several reports have previously described complexes of Rep proteins with AAV-2 capsids (23, 31, 32, 46).”
BackgroundMethodInfluentialSelf-citation
Mechanistic model for production of recombinant adeno-associated virus via triple transfection of HEK293 cells
Nguyen, T. N. T., Sha, S., Hong, M. S., Maloney, A. J., Barone, P. W., Neufeld, C., Wolfrum, J., Springs, S. L., Sinskey, A. J., & Braatz, R. D. (2021) · Molecular Therapy - Methods & Clinical Development · doi:10.1016/j.omtm.2021.04.006 · Cited by 107
“The Rep protein is postulated to detach from the capsid after packaging, as there has been evidence that Rep proteins accumulate mostly inside the nucleus.(58)”
Background
Integration of adeno-associated virus vectors in CD34+ human hematopoietic progenitor cells after transduction
Fisher-Adams, G., Wong, K. J., Podsakoff, G. M., Forman, S. J., & Chatterjee, S. (1996) · Blood · doi:10.1182/blood.v88.2.492.bloodjournal882492 · Cited by 103
Properties of the Adeno-Associated Virus Assembly-Activating Protein
Naumer, M., Sonntag, F., Schmidt, K., Nieto, K., Panke, C., Davey, N. E., Popa-Wagner, R., & Kleinschmidt, J. A. (2012) · Journal of Virology · doi:10.1128/jvi.01675-12 · Cited by 97
“assay using monoclonal antibody (MAb) B1 (24) or anti-AU1 (Covance,”
BackgroundMethodInfluentialSelf-citation
In vivo gene delivery to the lung using polyethylenimine and fractured polyamidoamine dendrimers
Rudolph, C., Lausier, J., Naundorf, S., M�ller, R. H., & Rosenecker, J. (2000) · The Journal of Gene Medicine · doi:10.1002/1521-2254(200007/08)2:4 · Cited by 194
Self-citation
Detection of intact rAAV particles up to 6 years after successful gene transfer in the retina of dogs and primates
Stieger, K., Schroeder, J., Provost, N., Mendes-Madeira, A., Belbellaa, B., Meur, G. L., Weber, M., Deschamps, J.-Y., Lorenz, B., Moullier, P., et al. (2009) · Molecular Therapy · doi:10.1038/mt.2008.283 · Cited by 88
Structure of adeno-associated virus-2 in complex with neutralizing monoclonal antibody A20
McCraw, D. M., O’Donnell, J. K., Taylor, K. A., Stagg, S. M., & Chapman, M. S. (2012) · Virology · doi:10.1016/j.virol.2012.05.004 · Cited by 84
“The complex of AAV-2 with Fab’ fragments of neutralizing monoclonal antibody A20 (Wistuba et al., 1995) was visualized through cryo-EM and reconstructed to a resolution of 8.”
BackgroundMethodInfluential
Understanding Capsid Assembly and Genome Packaging for Adeno-Associated Viruses
Bennett, A., Mietzsch, M., & Agbandje-McKenna, M. (2017) · Future Virology · doi:10.2217/fvl-2017-0011 · Cited by 82
The packaging capacity of adeno‐associated virus (AAV) and the potential for wild‐type‐plus AAV gene therapy vectors
Hermonat, P. L., Quirk, J. G., Bishop, B. M., & Han, L. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)00311-6 · Cited by 98
Identification of Cellular Proteins That Interact with the Adeno-Associated Virus Rep Protein
Nash, K. R., Chen, W., Salganik, M., & Muzyczka, N. (2008) · Journal of Virology · doi:10.1128/jvi.01939-08 · Cited by 82
“In the presence of helper virus, the two larger Rep proteins, Rep78 and Rep68, are required for AAV DNA replication (40, 48, 101), control of AAV transcription (9, 58, 60, 61, 66, 77, 111, 129), alternative splicing of viral RNA (86), viral DNA packaging (24, 31, 54, 83, 119), and site-specific integration of viral DN…”
BackgroundMethod
Generation and Characterization of Adeno-Associated Virus Producer Cell Lines for Research and Preclinical Vector Production
Martin, J. M., Frederick, A., Luo, Y., Jackson, R., Joubert, M., Sol, B., Poulin, F., Pastor, E., Armentano, D., Wadsworth, S. C., et al. (2013) · Human Gene Therapy Methods · doi:10.1089/hgtb.2013.046 · Cited by 79
“Interestingly, the Rep protein proﬁle differs from that observed in wtAd and wtAAV2 co-infected HeLa cells (Wistuba et al. , 1995).”
A 110-kDa Nuclear Shuttle Protein, Nucleolin, Specifically Binds to Adeno-Associated Virus Type 2 (AAV-2) Capsid
Qiu, J., & Brown, K. E. (1999) · Virology · doi:10.1006/viro.1999.9664 · Cited by 86
Development of AAV serotype-specific ELISAs using novel monoclonal antibodies
Kuck, D., Kern, A., & Kleinschmidt, J. A. (2007) · Journal of Virological Methods · doi:10.1016/j.jviromet.2006.10.005 · Cited by 74
Self-citation
Characterization of the Transcription Profile of Adeno-Associated Virus Type 5 Reveals a Number of Unique Features Compared to Previously Characterized Adeno-Associated Viruses
Qiu, J., Nayak, R., Tullis, G. E., & Pintel, D. J. (2002) · Journal of Virology · doi:10.1128/jvi.76.24.12435-12447.2002 · Cited by 71
“AAV5-infected (15 IU/ cell) or pAV5- or PCR-generated linear AAV5 DNA-transfected 293 cells were coinfected with Ad5 at 5 PFU/cell, and lysates were subjected to immunoblot analysis as previously described (20), using monoclonal antibody 303.9 raised to an amino-terminally truncated AAV2 Rep78 (42) (American Research…”
Influential
Structure–function analysis of receptor-binding in adeno-associated virus serotype 6 (AAV-6)
Xie, Q., Lerch, T. F., Meyer, N., & Chapman, M. S. (2011) · Virology · doi:10.1016/j.virol.2011.08.011 · Cited by 71
“Several monoclonal antibodies (mAbs) reacting with AAV capsids have been described (Hermens et al., 1999;Weger et al., 1997;Wistuba et al., 1995; Wobus et al., 2000).”
Background
Isolation of targeted AAV2 vectors from novel virus display libraries
Waterkamp, D. A., Müller, O. J., Ying, Y., Trepel, M., & Kleinschmidt, J. A. (2006) · The Journal of Gene Medicine · doi:10.1002/jgm.967 · Cited by 69
“for 1 h, the membranes were incubated for 1 h at room temperature with antibody B1 [43] (diluted 1 : 10 in blocking solution).”
MethodSelf-citation
Impact of Capsid Conformation and Rep-Capsid Interactions on Adeno-Associated Virus Type 2 Genome Packaging
Bleker, S., Pawlita, M., & Kleinschmidt, J. A. (2006) · Journal of Virology · doi:10.1128/jvi.80.2.810-820.2006 · Cited by 75
“It was previously shown that Rep proteins are associated with capsids in the presence of DNA packaging by either a direct or DNA-mediated interaction (33, 44).”
BackgroundSelf-citation
Nuclear Transport of Trimeric Assembly Intermediates Exerts a Morphogenetic Control on the Icosahedral Parvovirus Capsid
Riolobos, L., Reguera, J., Mateu, M. G., & Almendral, J. M. (2006) · Journal of Molecular Biology · doi:10.1016/j.jmb.2006.01.019 · Cited by 67
The Threefold Protrusions of Adeno-Associated Virus Type 8 Are Involved in Cell Surface Targeting as Well as Postattachment Processing
Raupp, C., Naumer, M., Müller, O. J., Gurda, B. L., Agbandje‐McKenna, M., & Kleinschmidt, J. A. (2012) · Journal of Virology · doi:10.1128/jvi.00209-12 · Cited by 64
Self-citation
Intranasal Vaccination with Recombinant Adeno-Associated Virus Type 5 against Human Papillomavirus Type 16 L1
Kuck, D., Lau, T., Leuchs, B., Kern, A., Müller, M., Gissmann, L., & Kleinschmidt, J. A. (2006) · Journal of Virology · doi:10.1128/jvi.80.6.2621-2630.2006 · Cited by 66
“powder and then incubated for 1 h at room temperature with antibody B1 (69)”
MethodSelf-citation
Cellular pathways of recombinant adeno-associated virus production for gene therapy
Sha, S., Maloney, A. J., Katsikis, G., Nguyen, T. N. T., Neufeld, C., Wolfrum, J., Barone, P. W., Springs, S. L., Manalis, S. R., Sinskey, A. J., et al. (2021) · Biotechnology Advances · doi:10.1016/j.biotechadv.2021.107764 · Cited by 67
The Assembly-Activating Protein Promotes Stability and Interactions between AAV’s Viral Proteins to Nucleate Capsid Assembly
Maurer, A. C., Pacouret, S., Diaz, A. K. C., Blake, J., Andrés‐Mateos, E., & Vandenberghe, L. H. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.04.026 · Cited by 64
“The current model for capsid assembly suggests that, after translation, AAV2 VP proteins oligomerize into trimers or pentamers, potentially in the cytoplasm, and that further assembly into the icosahedron occurs in the nucleolus (Wistuba et al., 1995, 1997).”
Background
In Vitro Packaging of Adeno-Associated Virus DNA
Zhou, X., & Muzyczka, N. (1998) · Journal of Virology · doi:10.1128/jvi.72.4.3241-3247.1998 · Cited by 63
“Studies of the subcellular distribution of AAV capsid proteins suggest that the three capsid proteins form a variety of complexes with sedimentation coefficients of 10S to 180S, with major peaks at approximately 66S and 110S, the positions of empty and mature virus particles (31).”
BackgroundResultInfluential
Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells
Große, S., Penaud‐Budloo, M., Herrmann, A.-K., Börner, K., Fakhiri, J., Laketa, V., Krämer, C., Wiedtke, E., Gunkel, M., Ménard, L., et al. (2017) · Journal of Virology · doi:10.1128/jvi.01198-17 · Cited by 62
“Kleinschmidt [49]), anti-HA (sc-7392; Santa Cruz Biotechnology), and anti- -actin (sc-47778; Santa Cruz Biotechnology) were diluted 1:30, 1:10, 1:300, and 1:1,000, respectively, in blocking buffer.”
BackgroundMethod
Encapsidation of adeno-associated virus type 2 Rep proteins in wild-type and recombinant progeny virions: Rep-mediated growth inhibition of primary human cells
Kube, D. M., Ponnazhagan, S., & Srivastava, A. (1997) · Journal of Virology · doi:10.1128/jvi.71.10.7361-7371.1997 · Cited by 58
“These results are consistent with a report demonstrating that complexes of AAV Rep and capsid proteins exist during a productive infection of HeLa cells with AAV and Ad2 (52).”
Result
Large-scale production, purification and crystallization of wild-type adeno-associated virus-2
Xie, Q., Hare, J. T., Turnigan, J., & Chapman, M. S. (2004) · Journal of Virological Methods · doi:10.1016/j.jviromet.2004.07.007 · Cited by 56
Production of adeno‐associated viral vectors in insect cells using triple infection: Optimization of baculovirus concentration ratios
Aucoin, M. G., Perrier, M., & Kamen, A. (2006) · Biotechnology and Bioengineering · doi:10.1002/bit.21069 · Cited by 56
“The pattern observed in our Westerns has been previously observed for the production of rAAV in mammalian cells (Grimm et al., 1998;Urabe et al., 2006;Wistuba et al., 1995) but never in the context of varying plasmid/virus concentrations.”
Background
Accurate Titration of Infectious AAV Particles Requires Measurement of Biologically Active Vector Genomes and Suitable Controls
François, A., Bouzelha, M., Lecomte, E., Broucque, F., Penaud-Budloo, M., Adjali, O., Moullier, P., Blouin, V., & Ayuso, E. (2018) · Molecular Therapy - Methods & Clinical Development · doi:10.1016/j.omtm.2018.07.004 · Cited by 50
“It also showed the complete absence of VP1 in AAV8DVP1-GFP preparation, which was further confirmed by western blot analysis with anti-VP1/2/3 antibody B1 (Figure 1B, lane 4).(22)”
Result
Control of adeno-associated virus type 2 cap gene expression: relative influence of helper virus, terminal repeats, and Rep proteins
Weger, S., Wistuba, A., Grimm, D., & Kleinschmidt, J. A. (1997) · Journal of Virology · doi:10.1128/jvi.71.11.8437-8447.1997 · Cited by 53
“However, the results of Rep-Cap double-immunofluorescence staining of HeLa cells infected with AAV-2 and adenovirus type 2 (MOI 5 10 and 20, respectively) using a combination of monoclonal Rep antibody 76/3, recognizing only Rep78 and Rep52 (64), and a polyclonal serum recognizing all three VP proteins showed a large…”
MethodInfluentialSelf-citation
Residues within the B′ Motif Are Critical for DNA Binding by the Superfamily 3 Helicase Rep40 of Adeno-associated Virus Type 2
Yoon-Robarts, M., Blouin, A. G., Bleker, S., Kleinschmidt, J. A., Aggarwal, A. K., Escalante, C. R., & Linden, R. M. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m403900200 · Cited by 52
Self-citation
Assembly of adeno-associated virus type 2 capsids in vitro.
Steinbach, S., Kleinschmidt, J. A., Wistuba, A., & Bock, T. (1997) · Journal of General Virology · doi:10.1099/0022-1317-78-6-1453 · Cited by 52
“(Wistuba et al., 1995) using monoclonal antibodies directed against AAV capsids (A20) or against capsid proteins (A69}A1) (Wistuba et al.”
InfluentialSelf-citation
A method for the preparation of highly purified adeno-associated virus using affinity column chromatography, protease digestion and solvent extraction
Anderson, R. W., Macdonald, I., Corbett, T., Whiteway, A., & Prentice, H. G. (2000) · Journal of Virological Methods · doi:10.1016/s0166-0934(99)00150-0 · Cited by 46
High-level expression of adeno-associated virus (AAV) Rep78 or Rep68 protein is sufficient for infectious-particle formation by a rep-negative AAV mutant
Hölscher, C., Kleinschmidt, J. A., & Bürkle, A. (1995) · Journal of Virology · doi:10.1128/jvi.69.11.6880-6885.1995 · Cited by 40
“, showing that Rep78 and Rep68 could be coprecipitated with different monoclonal antibodies against capsid proteins from fractionated extracts of HeLa cells productively infected with AAV and Ad2 (32).”
BackgroundInfluentialSelf-citation
Proteins attached to viral genomes are multifunctional
Sadowy, E., Miłner, M., & Haenni, A. (2001) · Advances in virus research · doi:10.1016/s0065-3527(01)57004-9 · Cited by 38
The Adeno-Associated Virus Rep78 Major Regulatory Protein Binds the Cellular TATA-Binding Proteinin Vitroandin Vivo
Hermonat, P. L., Santin, A. D., Batchu, R. B., & Zhan, D.-J. (1998) · Virology · doi:10.1006/viro.1998.9144 · Cited by 38
Adeno-associated virus type 5 exploits two different entry pathways in human embryo fibroblasts
Bantel‐Schaal, U., Braspenning‐Wesch, I., & Kartenbeck, J. (2009) · Journal of General Virology · doi:10.1099/vir.0.005595-0 · Cited by 39
Topors, a p53 and topoisomerase I binding protein, interacts with the adeno-associated virus (AAV-2) Rep78/68 proteins and enhances AAV-2 gene expression
Weger, S., Hammer, E., & Heilbronn, R. (2002) · Journal of General Virology · doi:10.1099/0022-1317-83-3-511 · Cited by 37
“9 (Wistuba et al., 1995) and B1 (Progen), respectively ; for AAV mRNA steady state levels by Northern blot analysis (C) with a 32Plabelled AAV-2 hybridization probe; and for Flag–Topors expression levels by Western blot analysis with the monoclonal anti-Flag antibody M2 (Sigma) (E).”
MethodInfluentialSelf-citation
Long-Term Genetic Modification of Rhesus Monkey Hematopoietic Cells Following Transplantation of Adenoassociated Virus Vector-Transduced CD34 + Cells
Schimmenti, S., Boesen, J. J. B., Claassen, E. A. W., Valerio, D., & Einerhand, M. P. W. (1998) · Human Gene Therapy · doi:10.1089/hum.1998.9.18-2727 · Cited by 36
“Virus particles accumulate in the nucleus of infected cells and are assembled in an interactive process involving both the rep and cap gene products (Ruffing et al, 1992; Wistuba et al, 1995).”
Recombinant Adeno-Associated Virus Vectors for Gene Therapy of the Central Nervous System: Delivery Routes and Clinical Aspects
Słyk, Ż., Stachowiak, N., & Małecki, M. (2024) · Biomedicines · doi:10.3390/biomedicines12071523 · Cited by 40
Avian Adeno-Associated Virus Vector Efficiently Transduces Neurons in the Embryonic and Post-Embryonic Chicken Brain
Matsui, R., Tanabe, Y., & Watanabe, D. (2012) · PLoS ONE · doi:10.1371/journal.pone.0048730 · Cited by 37
“The rep and cap ORFs encode four replication regulatory proteins and three capsid proteins, respectively [19].”
Background
Characterization of Adenovirus-Induced Inverted Terminal Repeat-Independent Amplification of Integrated Adeno-Associated Virus rep-cap Sequences
Tessier, J., Chadeuf, G., Nony, P., Avet‐Loiseau, H., Moullier, P., & Salvetti, A. (2001) · Journal of Virology · doi:10.1128/jvi.75.1.375-383.2001 · Cited by 35
“Forty-eight hours later, the cells were fixed and analyzed by immunofluorescence using an anti-DBP (26) and an anti-Rep 68/40 (A, B, and C) or anti-Rep 78/52 (D, E, and F) antibody (39).”
Method
Parvovirus Capsid-Antibody Complex Structures Reveal Conservation of Antigenic Epitopes Across the Family
Emmanuel, S. N., Mietzsch, M., Tseng, Y.-S., Smith, J. K., & Agbandje‐McKenna, M. (2020) · Viral Immunology · doi:10.1089/vim.2020.0022 · Cited by 33
“For this purpose, numerous mouse monoclonal antibodies have been generated against AAV serotypes for study of capsid-antibody interactions (55,59,71,117,124,130).”
Background
Insertional Mutagenesis at Positions 520 and 584 of Adeno-Associated Virus Type 2 (AAV2) Capsid Gene and Generation of AAV2 Vectors with Eliminated Heparin- Binding Ability and Introduced Novel Tropism
Shi, X., Fang, G., Shi, W., & Bartlett, J. S. (2006) · Human Gene Therapy · doi:10.1089/hum.2006.17.353 · Cited by 33
“The A20 monoclonal antibody detects both full and empty AAV particles (Wistuba et al ., 1995). rAAV2 vector was serially diluted and tested with the A20 ELISA kit.”
Identification of Rep-Associated Factors in Herpes Simplex Virus Type 1-Induced Adeno-Associated Virus Type 2 Replication Compartments
Nicolas, A., Alazard-Dany, N., Biollay, C., Arata, L., Jolinon, N., Kühn, L., Ferro, M., Weller, S. K., Epstein, A. L., Salvetti, A., et al. (2010) · Journal of Virology · doi:10.1128/jvi.00725-10 · Cited by 33
Chemical Modulation of Endocytic Sorting Augments Adeno-associated Viral Transduction
Berry, G. E., & Asokan, A. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.687657 · Cited by 29
“Anti-capsid protein antibody B1 (24) was used to blot for capsid protein, whereas anti-capsid antibody A20 (25) was used for immunoprecipitation and immunostaining.”
Method
Ring finger protein 121 is a potent regulator of adeno-associated viral genome transcription
Madigan, V. J., Yuziuk, J. A., Chiarella, A. M., Tyson, T. O., Meganck, R. M., Elmore, Z. C., Tse, L. V., Hathaway, N. A., & Asokan, A. (2019) · PLoS Pathogens · doi:10.1371/journal.ppat.1007988 · Cited by 28
Generation and characterization of anti-Adeno-associated virus serotype 8 (AAV8) and anti-AAV9 monoclonal antibodies
Tseng, Y.-S., Vliet, K. V., Rao, L., McKENNA, R. V., Byrne, B. J., Asokan, A., & Agbandje‐McKenna, M. (2016) · Journal of Virological Methods · doi:10.1016/j.jviromet.2016.07.009 · Cited by 28
“In this assay, ADK4, a MAb that specifically recognizes AAV4 (Kuck et al. 2007) was used as a negative control while ADK8 and ADK9, known to neutralize infection by AAV8 (Gurda et al. 2012) and AAV9 (Sonntag et al. 2011), respectively, were used as positive control.”
MethodInfluential
The Golgi Calcium ATPase Pump Plays an Essential Role in Adeno-associated Virus Trafficking and Transduction
Madigan, V. J., Berry, G. E., Tyson, T. O., Nardone-White, D. T., Ark, J., Elmore, Z. C., Murlidharan, G., Vincent, H. A., & Asokan, A. (2020) · Journal of Virology · doi:10.1128/jvi.01604-20 · Cited by 25
“Briefly, Scr and ATP2C1 KO cells were transduced with AAV2 for 18 h and then subjected to immunofluorescent staining with the A20 antibody, which recognizes intact AAV2 capsids in their native conformation (13).”
Method
The Cellular TATA Binding Protein Is Required for Rep-Dependent Replication of a Minimal Adeno-Associated Virus Type 2 p5 Element
François, A., Guilbaud, M., Awedikian, R., Chadeuf, G., Moullier, P., & Salvetti, A. (2005) · Journal of Virology · doi:10.1128/jvi.79.17.11082-11094.2005 · Cited by 27
Development of a Two-Dimensional Liquid Chromatography-Mass Spectrometry Platform for Simultaneous Multi-Attribute Characterization of Adeno-Associated Viruses
Wu, Z., Wang, H., Tustian, A. D., Qiu, H., & Li, N. (2022) · Analytical Chemistry · doi:10.1021/acs.analchem.1c04873 · Cited by 27
Herpes Simplex Virus Type 1 ICP0 Protein Mediates Activation of Adeno-Associated Virus Type 2 rep Gene Expression from a Latent Integrated Form
Geoffroy, M., Epstein, A. L., Toublanc, E., Moullier, P., & Salvetti, A. (2004) · Journal of Virology · doi:10.1128/jvi.78.20.10977-10986.2004 · Cited by 23
“Rep proteins or only unspliced Rep proteins, respectively (81, 82).”
Background
Nuclear translocation of adeno-associated virus type 2 capsid proteins for virion assembly
Popa-Wagner, R., Sonntag, F., Schmidt, K., King, J., & Kleinschmidt, J. A. (2012) · Journal of General Virology · doi:10.1099/vir.0.043232-0 · Cited by 24
“11 On: Sun, 16 Dec 2018 00:40:20 capsid proteins VP1, VP2 and VP3 was analysed from whole-cell extracts by Western blotting using the mAb B1 (Wistuba et al., 1995) and expression of VP2 N terminus–GFP fusion proteins using the mAb A69 (Wistuba et al.”
MethodSelf-citation
Efficiency of HPV 16 L1/E7 DNA immunization: Influence of cellular localization and capsid assembly
Kuck, D., Leder, C., Kern, A., Müller, M., Piuko, K., Gissmann, L., & Kleinschmidt, J. A. (2005) · Vaccine · doi:10.1016/j.vaccine.2005.12.023 · Cited by 22
Self-citation
Characterization of the Rep78/Adeno-Associated Virus Complex
Prasad, K. M. R., Zhou, C., & Trempe, J. P. (1997) · Virology · doi:10.1006/viro.1996.8431 · Cited by 21
Towards a Platform Chromatography Purification Process for Adeno‐Associated Virus (AAV)
Bogdanovic, A., Donohue, N., Glennon, B., McDonnell, S., & Whelan, J. (2025) · Biotechnology Journal · doi:10.1002/biot.202400526 · Cited by 19
Subcellular localization of Aleutian mink disease parvovirus proteins and DNA during permissive infection of Crandell feline kidney cells
Oleksiewicz, M. B., Costello, F., Huhtanen, M. E., Wolfinbarger, J. B., Alexandersen, S., & Bloom, M. E. (1996) · Journal of Virology · doi:10.1128/jvi.70.5.3242-3247.1996 · Cited by 18
“However, whether the empty capsids commonly observed in infected nuclei serve as precursors for full capsids or whether full and empty capsids arise from unassembled capsid proteins by parallel pathways remains undetermined (38, 41).”
Background
A Comparative Analysis of the Structural Architecture of ssDNA Viruses
Bennett, A. D., McKENNA, R. V., & Agbandje‐McKenna, M. (2008) · Computational and Mathematical Methods in Medicine · doi:10.1080/17486700802168247 · Cited by 19
“For AAV2 10-15S, subassembly complexes containing predominantly VP2 and VP3 consistent with pentamers have been isolated in addition to the disruption of capsid assembly for VP mutations that change amino acids involved in 5-fold interactions [14,37,38].”
Background
Radioprotective gene therapy
Maier, P., Veldwijk, M. R., & Wenz, F. K. (2011) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.2011.580271 · Cited by 18
“The wild type AAV genome contains three open reading frames (ORFs): the first encodes the four non-structural Rep proteins that control and regulate AAV replication [40], whereas the second encodes the three viral capsid proteins (Figure 2) [41,42].”
Background
The adeno‐associated virus Rep78 major regulatory protein forms multimeric complexes and the domain for this activity is contained within the carboxy‐half of the molecule
Hermonat, P. L., & Batchu, R. B. (1997) · FEBS Letters · doi:10.1016/s0014-5793(96)01469-x · Cited by 17
Biophysical characterization of adeno-associated virus capsid through the viral transduction life cycle
Xiang, Y. S., & Hao, G. G. (2023) · Journal of Genetic Engineering and Biotechnology · doi:10.1186/s43141-023-00518-5 · Cited by 18
“Sucrose density sedimentation experiments showed that VPs oligomerize in the cytoplasm (trimers or pentamers), but do not form capsids [59].”
Background
Structural and Biophysical Analysis of Adeno-Associated Virus Serotype 2 Capsid Assembly Variants
Bennett, A. D., Gargas, J., Kansol, A., Lewis, J., Hsi, J., Hull, J. A., Mietzsch, M., Tartaglia, L. J., Muzyczka, N., Bhattacharya, N., et al. (2023) · Journal of Virology · doi:10.1128/jvi.01772-22 · Cited by 19

Authors: Wistuba, A., Weger, S., Kern, A., & Kleinschmidt, J. A.
Affiliations: German Cancer Research Center (DE)
ORCID: Weger, S
Venue: Journal of Virology
Publisher: American Society for Microbiology
Published: 1995-09
Volume: 69
Issue: 9
Pages: 5311-5319
ISSN: 0022-538X (ISSN-L)
1098-5514
DOI: 10.1128/jvi.69.9.5311-5319.1995
PMC: PMC189369
OpenAlex: W2172045470
Cited by: 147
References: 44
Citations by year: 2026: 3 · 2025: 4 · 2024: 5 · 2023: 6 · 2022: 2 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 6 · 2017: 4 · 2016: 2 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 7
FWCI: 3.75
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Journal of Virology (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Health Sciences › Medicine › Infectious Diseases › Parvovirus B19 Infection Studies
Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
Keywords: adeno-associated virus type 2, capsid assembly, Rep protein, capsid protein, capsid maturation, DNA encapsidation, Rep78, Rep68, co-immunoprecipitation, sedimentation analysis
MeSH terms: Dependovirus, Capsid, Cell Fractionation, Cell Nucleus, Cytoplasm, Electrophoresis, Polyacrylamide Gel, Genes, Viral, HeLa Cells, Humans, Virus Replication, Immunoblotting, Viral Nonstructural Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks