The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
West Nile Virus Recombinant DNA Vaccine Protects Mouse and Horse from Virus Challenge and Expresses In Vitro a Noninfectious Recombinant Antigen That Can Be Used in Enzyme-Linked Immunosorbent Assays
Open accessAbstract
Introduction of West Nile (WN) virus into the United States in 1999 created major human and animal health concerns. Currently, no human or veterinary vaccine is available to prevent WN viral infection, and mosquito control is the only practical strategy to combat the spread of disease. Starting with a previously designed eukaryotic expression vector, we constructed a recombinant plasmid (pCBWN) that expressed the WN virus prM and E proteins. A single intramuscular injection of pCBWN DNA induced protective immunity, preventing WN virus infection in mice and horses. Recombinant plasmid-transformed COS-1 cells expressed and secreted high levels of WN virus prM and E proteins into the culture medium. The medium was treated with polyethylene glycol to concentrate proteins. The resultant, containing high-titered recombinant WN virus antigen, proved to be an excellent alternative to the more traditional suckling-mouse brain WN virus antigen used in the immunoglobulin M (IgM) antibody-capture and indirect IgG enzyme-linked immunosorbent assays. This recombinant antigen has great potential to become the antigen of choice and will facilitate the standardization of reagents and implementation of WN virus surveillance in the United States and elsewhere.
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Cites
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Cited by
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Richner, J. M., Himansu, S., Dowd, K. A., Butler, S. L., Salazar, V., Fox, J. M., Julander, J. G., Tang, W. W., Shresta, S., Pierson, T. C., et al. (2017) · Cell · doi:10.1016/j.cell.2017.02.017 · Cited by 909
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Delany, I., Rappuoli, R., & De Gregorio, E. (2014) · EMBO Molecular Medicine · doi:10.1002/emmm.201403876 · Cited by 420
Rapid development of a DNA vaccine for Zika virus
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Localization and Characterization of Flavivirus Envelope Glycoprotein Cross-Reactive Epitopes
Crill, W. D., & Chang, G.-J. J. (2004) · Journal of Virology · doi:10.1128/jvi.78.24.13975-13986.2004 · Cited by 300
Self-citation
Metadata
- Authors
- Davis, B. S., Chang, G.-J. J., Cropp, B., Roehrig, J. T., Martin, D. A., Mitchell, C. J., Bowen, R., & Bunning, M. L.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 2001-05
- Volume
- 75
- Issue
- 9
- Pages
- 4040-4047
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2033094913
- Cited by
- 447
- References
- 32
- FWCI
- 26.57
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: West Nile Virus Recombinant DNA Vaccine Protects Mouse and Horse from Virus Challenge and Expresses In Vitro a Noninfectious Recombinant Antigen That Can Be Used in Enzyme-Linked Immunosorbent Assays. https://app.cochat.ai/papers/10.1128/jvi.75.9.4040-4047.2001
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- Davis, B. S., Chang, G.-J. J., Cropp, B., Roehrig, J. T., Martin, D. A., Mitchell, C. J., Bowen, R., & Bunning, M. L. (2001). West Nile Virus Recombinant DNA Vaccine Protects Mouse and Horse from Virus Challenge and Expresses In Vitro a Noninfectious Recombinant Antigen That Can Be Used in Enzyme-Linked Immunosorbent Assays. Journal of Virology. https://doi.org/10.1128/jvi.75.9.4040-4047.2001
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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