Bacterial topography of the healthy human lower respiratory tract
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ABSTRACT Although culture-independent techniques have refuted lung sterility in health, controversy about contamination during bronchoscope passage through the upper respiratory tract (URT) has impeded research progress. We sought to establish whether bronchoscopic sampling accurately reflects the lung microbiome in health and to distinguish between two proposed routes of authentic microbial immigration, (i) dispersion along contiguous respiratory mucosa and (ii) subclinical microaspiration. During bronchoscopy of eight adult volunteers without lung disease, we performed seven protected specimen brushings (PSB) and bilateral bronchoalveolar lavages (BALs) per subject. We amplified, sequenced, and analyzed the bacterial 16S rRNA gene V4 regions by using the Illumina MiSeq platform. Rigorous attention was paid to eliminate potential sources of error or contamination, including a randomized processing order and the inclusion and analysis of exhaustive procedural and sequencing control specimens. Indices of mouth-lung immigration (mouth-lung community similarity, bacterial burden, and community richness) were all significantly greater in airway and alveolar specimens than in bronchoscope contamination control specimens, indicating minimal evidence of pharyngeal contamination. Ecological indices of mouth-lung immigration peaked at or near the carina, as predicted for a primary immigration route of microaspiration. Bacterial burden, diversity, and mouth-lung similarity were greater in BAL than PSB samples, reflecting differences in the sampled surface areas. (This study has been registered at ClinicalTrials.gov under registration no. NCT02392182.) IMPORTANCE This study defines the bacterial topography of the healthy human respiratory tract and provides ecological evidence that bacteria enter the lungs in health primarily by microaspiration, with potential contribution in some subjects by direct dispersal along contiguous mucosa. By demonstrating that contamination contributes negligibly to microbial communities in bronchoscopically acquired specimens, we validate the use of bronchoscopy to investigate the lung microbiome.
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In healthy individuals, the microbial community richness is lower in the LRT than that in the URT.
“In healthy individuals, the microbial community richness (i.e., the total number of bacterial taxa) is lower in the LRT than that in the URT”
From Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia. (2018) · cites this paper as [74]
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R: A Language and Environment for Statistical Computing
R Core Team (2000) · doi:10.32614/r.manuals · Cited by 355,706 · see citing works
A new look at the statistical model identification
Akaike, H. (1974) · IEEE Transactions on Automatic Control · doi:10.1109/tac.1974.1100705 · Cited by 51,879 · see citing works
Schloss, P. D., Westcott, S. L., Ryabin, T., Hall, J. R., Hartmann, M., Hollister, E. B., Lesniewski, R. A., Oakley, B. B., Parks, D. H., Robinson, C. J., et al. (2009) · Applied and Environmental Microbiology · doi:10.1128/aem.01541-09 · Cited by 20,966 · see citing works
Complex heatmaps reveal patterns and correlations in multidimensional genomic data
Gu, Z., Eils, R., & Schlesner, M. (2016) · Bioinformatics · doi:10.1093/bioinformatics/btw313 · Cited by 10,122 · see citing works
Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample
Caporaso, J. G., Lauber, C. L., Walters, W. A., Berg-Lyons, D., Lozupone, C., Turnbaugh, P. J., Fierer, N., & Knight, R. D. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1000080107 · Cited by 9,633 · see citing works
Kozich, J. J., Westcott, S. L., Baxter, N. T., Highlander, S. K., & Schloss, P. D. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.01043-13 · Cited by 7,004 · see citing works
Vegan : community ecology package. R package version 1.17-9
Oksanen, J. (2007) · Medical Entomology and Zoology · Cited by 6,606 · see citing works
(1980) · Annals of Internal Medicine · doi:10.7326/0003-4819-92-3-460_4 · Cited by 4,593 · see citing works
Reagent and laboratory contamination can critically impact sequence-based microbiome analyses
Salter, S. J., Cox, M. J., Turek, E. M., Calus, S. T., Cookson, W. O., Moffatt, M. F., Turner, P., Parkhill, J., Loman, N. J., & Walker, A. W. (2014) · BMC Biology · doi:10.1186/s12915-014-0087-z · Cited by 3,463 · see citing works
Disordered Microbial Communities in Asthmatic Airways
Hilty, M., Burke, C. M., Pedro, H., Cárdenas, P., Bush, A., Bossley, C. J., Davies, J. C., Ervine, A., Poulter, L. W., Pachter, L. S., et al. (2010) · PLoS ONE · doi:10.1371/journal.pone.0008578 · Cited by 1,825 · see citing works
mvabund– an R package for model‐based analysis of multivariate abundance data
Wang, Y., Naumann, U., Wright, S. T., & Warton, D. I. (2012) · Methods in Ecology and Evolution · doi:10.1111/j.2041-210x.2012.00190.x · Cited by 1,587 · see citing works
Topographical Continuity of Bacterial Populations in the Healthy Human Respiratory Tract
Charlson, E. S., Bittinger, K., Haas, A. R., Fitzgerald, A. S., Frank, I., Yadav, A., Bushman, F. D., & Collman, R. G. (2011) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201104-0655oc · Cited by 1,144 · see citing works
Analysis of the lung microbiome in the “healthy” smoker and in COPD.
Erb-Downward, J. R., Thompson, D. L., Han, M. K., Freeman, C. M., McCloskey, L., Schmidt, L. A., Young, V. B., Toews, G. B., Curtis, J. L., Sundaram, B., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016384 · Cited by 973 · see citing works
Self-citationThe Microbiome and the Respiratory Tract
Dickson, R. P., Erb-Downward, J. R., Martinez, F. J., & Huffnagle, G. B. (2016) · Annual Review of Physiology · doi:10.1146/annurev-physiol-021115-105238 · Cited by 953 · see citing works
Self-citationBassis, C. M., Erb-Downward, J. R., Dickson, R. P., Freeman, C. M., Schmidt, T. M., Young, V. B., Beck, J. M., Curtis, J. L., & Huffnagle, G. B. (2015) · mBio · doi:10.1128/mbio.00037-15 · Cited by 839 · see citing works
Self-citationComparison of the Respiratory Microbiome in Healthy Nonsmokers and Smokers
Morris, A., Beck, J. M., Schloss, P. D., Campbell, T. B., Crothers, K., Curtis, J. L., Flores, S. C., Fontenot, A. P., Ghedin, E., Huang, L., et al. (2013) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201210-1913oc · Cited by 822 · see citing works
Self-citationPharyngeal aspiration in normal adults and patients with depressed consciousness
Huxley, E. J., Viroslav, J., Gray, W. R., & Pierce, A. K. (1978) · The American Journal of Medicine · doi:10.1016/0002-9343(78)90574-0 · Cited by 739 · cited 1× · see citing works
“onstrated repeatedly by using radiographic techniques [17, 33, 34]).”
Goodrich, J. K., Di Rienzi, S. C., Poole, A. C., Koren, O., Walters, W. A., Caporaso, J. G., Knight, R., & Ley, R. E. (2014) · Cell · doi:10.1016/j.cell.2014.06.037 · Cited by 714 · see citing works
Segal, L. N., Clemente, J. C., Tsay, J.-C. J., Koralov, S. B., Keller, B. C., Wu, B. G., Li, Y., Shen, N., Ghedin, E., Morris, A., et al. (2016) · Nature Microbiology · doi:10.1038/nmicrobiol.2016.31 · Cited by 685 · see citing works
Pharyngeal aspiration in normal adults and patients with depressed consciousness
(1978) · The American Journal of Medicine · doi:10.1016/0002-9343(78)90607-1 · Cited by 607 · see citing works
Dickson, R. P., Erb-Downward, J. R., Freeman, C. M., McCloskey, L., Beck, J. M., Huffnagle, G. B., & Curtis, J. L. (2015) · Annals of the American Thoracic Society · doi:10.1513/annalsats.201501-029oc · Cited by 567 · see citing works
Self-citationThe Lung Microbiome: New Principles for Respiratory Bacteriology in Health and Disease
Dickson, R. P., & Huffnagle, G. B. (2015) · PLoS Pathogens · doi:10.1371/journal.ppat.1004923 · Cited by 562 · see citing works
Self-citationThe Lung Tissue Microbiome in Chronic Obstructive Pulmonary Disease
Sze, M. A., Dimitriu, P. A., Hayashi, S., Elliott, W. M., McDonough, J. E., Gosselink, J. V., Cooper, J. D., Sin, D. D., Mohn, W. W., & Hogg, J. C. (2012) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201111-2075oc · Cited by 552 · see citing works
Robbins Pathologic Basis of Disease
Chejfec, G. (1999) · Archives of Pathology & Laboratory Medicine · doi:10.5858/1999-123-859a-rpbod · Cited by 517 · see citing works
The role of the microbiome in exacerbations of chronic lung diseases
Dickson, R. P., Martinez, F. J., & Huffnagle, G. B. (2014) · The Lancet · doi:10.1016/s0140-6736(14)61136-3 · Cited by 495 · see citing works
Self-citationSegal, L. N., Alekseyenko, A. V., Clemente, J. C., Kulkarni, R., Wu, B., Chen, H., Berger, K. I., Goldring, R. M., Rom, W. N., Blaser, M. J., et al. (2013) · Microbiome · doi:10.1186/2049-2618-1-19 · Cited by 482 · see citing works
Quantitative Aspiration During Sleep in Normal Subjects
Gleeson, K., Maxwell, S. L., & Eggli, D. F. (1997) · CHEST Journal · doi:10.1378/chest.111.5.1266 · Cited by 448 · see citing works
Application of a Neutral Community Model To Assess Structuring of the Human Lung Microbiome
Venkataraman, A., Bassis, C. M., Beck, J. M., Young, V. B., Curtis, J. L., Huffnagle, G. B., & Schmidt, T. M. (2015) · mBio · doi:10.1128/mbio.02284-14 · Cited by 428 · see citing works
Self-citationWimberley, N., Faling, L. J., & Bartlett, J. G. (1979) · PubMed · doi:10.1164/arrd.1979.119.3.337 · Cited by 408 · see citing works
Han, M. K., Zhou, Y., Murray, S., Tayob, N., Noth, I., Lama, V. N., Moore, B. B., White, E. S., Flaherty, K. R., Hu, B., et al. (2014) · The Lancet Respiratory Medicine · doi:10.1016/s2213-2600(14)70069-4 · Cited by 408 · see citing works
Dickson, R. P., Erb-Downward, J. R., & Huffnagle, G. B. (2014) · The Lancet Respiratory Medicine · doi:10.1016/s2213-2600(14)70028-1 · Cited by 371 · see citing works
Self-citationDickson, R. P., Erb‐Downward, J. R., Freeman, C. M., Walker, N., Scales, B. S., Beck, J. M., Martínez, F. J., Curtis, J. L., Lama, V. N., & Huffnagle, G. B. (2014) · PLoS ONE · doi:10.1371/journal.pone.0097214 · Cited by 203 · see citing works
Self-citationEpidemiology of Ventilator-acquired Pneumonia Based on Protected Bronchoscopic Sampling
George, D. L., Falk, P. S., Wunderink, R. G., Leeper, K. V., Meduri, G. U., Steere, E. L., Corbett, C., & Mayhall, C. G. (1998) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.158.6.9610069 · Cited by 196 · see citing works
Charlson, E. S., Bittinger, K. L., Chen, J., Diamond, J. M., Li, H., Collman, R. G., & Bushman, F. D. (2012) · PLoS ONE · doi:10.1371/journal.pone.0042786 · Cited by 160 · see citing works
The internal surface area of the adult human lung.
Hasleton, P. S. (1972) · PubMed · Cited by 107 · see citing works
THE RELATIONSHIP OF SINUSITIS AND BRONCHIECTASIS
QUINN, L. H., & MEYER, O. O. (1929) · Archives of Otolaryngology - Head and Neck Surgery · doi:10.1001/archotol.1929.00620050048003 · Cited by 96 · see citing works
Cell-associated bacteria in the human lung microbiome
Dickson, R. P., Erb‐Downward, J. R., Prescott, H. C., Martínez, F. J., Curtis, J. L., Lama, V. N., & Huffnagle, G. B. (2014) · Microbiome · doi:10.1186/2049-2618-2-28 · Cited by 89 · see citing works
Self-citationInvestigative Bronchoprovocation and Bronchoscopy in Airway Diseases
Busse, W. W., Wanner, A., Adams, K., Reynolds, H. Y., Castro, M., Chowdhury, B. A., Kraft, M., Levine, R. J., Peters, S. P., & Sullivan, E. J. (2005) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200407-966ws · Cited by 82 · see citing works
Möller, D. R., Koth, L. L., Maier, L. A., Morris, A., Drake, W. P., Rossman, M. D., Leader, J. K., Collman, R. G., Hamzeh, N. Y., Sweiss, N., et al. (2015) · Annals of the American Thoracic Society · doi:10.1513/annalsats.201503-172ot · Cited by 81 · see citing works
Safety of investigative bronchoscopy in the Severe Asthma Research Program
Moore, W. C., Evans, M. D., Bleecker, E. R., Busse, W. W., Calhoun, W. J., Castro, M., Chung, K. F., ERZURUM, S. C., Curran‐Everett, D., Dweik, R. A., et al. (2011) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2011.02.042 · Cited by 78 · see citing works
Boersma, W. G., Erjavec, Z., van der Werf, T. S., de Vries-Hosper, H. G., Gouw, A. S. H., & Manson, W. L. (2006) · Respiratory Medicine · doi:10.1016/j.rmed.2006.04.021 · Cited by 68 · see citing works
The lung microbiome and exacerbations of COPD
Dy, R. V., & Sethi, S. (2016) · Current Opinion in Pulmonary Medicine · doi:10.1097/mcp.0000000000000268 · Cited by 60 · see citing works
Freeman, C. M., Crudgington, S., Stolberg, V. R., Brown, J. P., Sonstein, J., Alexis, N. E., Doerschuk, C. M., Basta, P. V., Carretta, E. E., Couper, D. J., et al. (2015) · Journal of Translational Medicine · doi:10.1186/s12967-014-0374-z · Cited by 48 · see citing works
Self-citationPresence of Tropheryma whipplei in Different Body Sites in a Cohort of Healthy Subjects
Qin, S., Clausen, E. S., Lucht, L. A., Michael, H., Beck, J. M., Curtis, J. L., Freeman, C. M., & Morris, A. M. (2016) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201601-0162le · Cited by 25 · see citing works
Self-citationStrange, C., Senior, R. M., Sciurba, F. C., O’Neal, S., Morris, A. M., Wisniewski, S. R., Bowler, R. P., Hochheiser, H., Becich, M. J., Zhang, Y., et al. (2015) · Annals of the American Thoracic Society · doi:10.1513/annalsats.201503-143oc · Cited by 16 · see citing works
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The Role of Lung and Gut Microbiota in the Pathology of Asthma
Barcik, W., Boutin, R. C. T., Sokołowska, M., & Finlay, B. B. (2020) · Immunity · doi:10.1016/j.immuni.2020.01.007 · Cited by 618 · see citing works
The dynamic lung microbiome in health and disease
Natalini, J. G., Singh, S., & Segal, L. N. (2022) · Nature Reviews Microbiology · doi:10.1038/s41579-022-00821-x · Cited by 528 · see citing works
Analysis of Gut Microbiota in Patients with Parkinson’s Disease
Petrov, V. A., Saltykova, I. V., Zhukova, I. A., Alifirova, V. M., Zhukova, N. G., Dorofeeva, Y. B., Tyakht, A. V., Kovarsky, B. A., Alekseev, D. G., Kostryukova, E. S., et al. (2017) · Bulletin of Experimental Biology and Medicine · doi:10.1007/s10517-017-3700-7 · Cited by 487 · see citing works
Respiratory viral infection-induced microbiome alterations and secondary bacterial pneumonia.
Hanada, S., Pirzadeh, M., Carver, K. Y., & Deng, J. C. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02640 · Cited by 495 · see citing works
Functional effects of the microbiota in chronic respiratory disease
Budden, K. F., Shukla, S. D., Rehman, S. F., Bowerman, K. L., Keely, S., Hugenholtz, P., Armstrong-James, D. P. H., Adcock, I. M., Chotirmall, S. H., Chung, K. F., et al. (2019) · The Lancet Respiratory Medicine · doi:10.1016/s2213-2600(18)30510-1 · Cited by 450 · see citing works
The role of the lung microbiota and the gut–lung axis in respiratory infectious diseases
Dumas, A., Bernard, L., Poquet, Y., Lugo-Villarino, G., & Neyrolles, O. (2018) · Cellular Microbiology · doi:10.1111/cmi.12966 · Cited by 444 · see citing works
Lung microbiome: new insights into the pathogenesis of respiratory diseases
Li, R., Li, J., & Zhou, X. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01722-y · Cited by 466 · see citing works
Langelier, C., Kalantar, K. L., Moazed, F., Wilson, M. R., Crawford, E. D., Deiss, T., Belzer, A., Bolourchi, S., Caldera, S., Fung, M., et al. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1809700115 · Cited by 411 · see citing works
Diet, Microbiota and Gut-Lung Connection
Anand, S., & Mande, S. S. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02147 · Cited by 409 · see citing works
Pathogenesis of pneumonia and acute lung injury
Long, M. E., Mallampalli, R. K., & Horowitz, J. C. (2022) · Clinical Science · doi:10.1042/cs20210879 · Cited by 410 · see citing works
Lung Microbiota Predict Clinical Outcomes in Critically Ill Patients
Dickson, R. P., Schultz, M. J., van der Poll, T., Schouten, L. R. A., Falkowski, N. R., Luth, J. E., Sjoding, M. W., Brown, C. A., Chanderraj, R., Huffnagle, G. B., et al. (2020) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201907-1487oc · Cited by 365 · see citing works
Self-citationThe role of the microbiota in infectious diseases
Libertucci, J., & Young, V. B. (2018) · Nature Microbiology · doi:10.1038/s41564-018-0278-4 · Cited by 360 · see citing works
Commensal Fungi in Health and Disease
Limon, J. J., Skalski, J. H., & Underhill, D. M. (2017) · Cell Host & Microbe · doi:10.1016/j.chom.2017.07.002 · Cited by 336 · see citing works
Lower Airway Dysbiosis Affects Lung Cancer Progression
Tsay, J.-C. J., Wu, B. G., Sulaiman, I., Gershner, K., Schluger, R., Li, Y., Yie, T.-A., Meyn, P., Olsen, E., Perez, L., et al. (2021) · Cancer Discovery · doi:10.1158/2159-8290.cd-20-0263 · Cited by 322 · see citing works
At the Root: Defining and Halting Progression of Early Chronic Obstructive Pulmonary Disease
Martínez, F. J., Han, M. K., Allinson, J. P., Barr, R. G., Boucher, R. C., Calverley, P. M. A., Celli, B. R., Christenson, S. A., Crystal, R. G., Fagerås, M., et al. (2018) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201710-2028pp · Cited by 284 · see citing works
Self-citationYildiz, S., Mazel-Sanchez, B., Kandasamy, M., Manicassamy, B., & Schmolke, M. (2018) · Microbiome · doi:10.1186/s40168-017-0386-z · Cited by 264 · see citing works
Theis, K. R., Romero, R. J., Winters, A. D., Greenberg, J. M., Gomez‐Lopez, N., Alhousseini, A., Bieda, J., Maymon, E., Pacora, P., Fettweis, J. M., et al. (2019) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2018.10.018 · Cited by 254 · see citing works
Dickson, R. P., Erb‐Downward, J. R., Falkowski, N. R., Hunter, E. M., Ashley, S. L., & Huffnagle, G. B. (2018) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201711-2180oc · Cited by 244 · see citing works
Self-citationLiu, H., Tao, L., Zhang, J., Zhu, Y., Zheng, Y., Liu, D., Zhou, M., Ke, H., Shi, M., & Qu, J. (2018) · International Journal of Cancer · doi:10.1002/ijc.31098 · Cited by 235 · see citing works
The human microbiome in disease and pathology
Manos, J. (2022) · Apmis · doi:10.1111/apm.13225 · Cited by 231 · see citing works
Man, W. H., van Houten, M. A., Mérelle, M. E., Vlieger, A. M., Chu, M. L. J. N., Jansen, N. J. G., Sanders, E. A. M., & Bogaert, D. (2019) · The Lancet Respiratory Medicine · doi:10.1016/s2213-2600(18)30449-1 · Cited by 213 · see citing works
The potential application of probiotics and prebiotics for the prevention and treatment of COVID-19
Olaimat, A. N., Aolymat, I., Al‐Holy, M., Ayyash, M. M., Abughoush, M. H., Al‐Nabulsi, A. A., Osaili, T. M. I., Apostolopoulos, V., Liu, S., & Shah, N. P. (2020) · npj Science of Food · doi:10.1038/s41538-020-00078-9 · Cited by 215 · see citing works
The lung microbiome in health and disease
Moffatt, M. F., & Cookson, W. (2017) · Clinical Medicine · doi:10.7861/clinmedicine.17-6-525 · Cited by 204 · see citing works
The lung microbiome: progress and promise
Whiteside, S. A., McGinniss, J. E., & Collman, R. G. (2021) · Journal of Clinical Investigation · doi:10.1172/jci150473 · Cited by 194 · see citing works
Methods in Lung Microbiome Research
Carney, S. M., Clemente, J. C., Cox, M. J., Dickson, R. P., Huang, Y. J., Kitsios, G. D., Kloepfer, K. M., Leung, J. M., LeVan, T. D., Molyneaux, P. L., et al. (2019) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2019-0273tr · Cited by 189 · see citing works
Self-citationThe Human Respiratory System and its Microbiome at a Glimpse
Santacroce, L., Charitos, I. A., Ballini, A., Inchingolo, F., Luperto, P., Nitto, E. D., & Topi, S. (2020) · Biology · doi:10.3390/biology9100318 · Cited by 186 · see citing works
Oral-lung microbiome interactions in lung diseases.
Mammen, M. J., Scannapieco, F. A., & Sethi, S. (2020) · Periodontology 2000 · doi:10.1111/prd.12301 · Cited by 179 · see citing works
Poh, T. Y., Ali, N. A. B. M., Aogáin, M. M., Kathawala, M. H., Setyawati, M. I., Ng, K. W., & Chotirmall, S. H. (2018) · Particle and Fibre Toxicology · doi:10.1186/s12989-018-0282-0 · Cited by 172 · see citing works
Ashley, S. L., Sjoding, M. W., Popova, A. P., Cui, T. X., Hoostal, M. J., Schmidt, T. M., Branton, W. R., Dieterle, M. G., Falkowski, N. R., Baker, J. M., et al. (2020) · Science Translational Medicine · doi:10.1126/scitranslmed.aau9959 · Cited by 170 · see citing works
Self-citationMuhlebach, M. S., Zorn, B., Esther, C. R., Hatch, J., Murray, C. P. J., Turkovic, L., Ranganathan, S., Boucher, R. C., Stick, S. M., & Wolfgang, M. C. (2018) · PLoS Pathogens · doi:10.1371/journal.ppat.1006798 · Cited by 165 · see citing works
The lung tissue microbiota of mild and moderate chronic obstructive pulmonary disease
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Early Life Microbiota and Respiratory Tract Infections
Piters, W. A. A. D. S., Binkowska, J. M., & Bogaert, D. (2020) · Cell Host & Microbe · doi:10.1016/j.chom.2020.07.004 · Cited by 148 · see citing works
The gut–airway microbiome axis in health and respiratory diseases
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Microbiome dysbiosis in lung cancer: from composition to therapy
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Ramsheh, M. Y., Haldar, K., Esteve‐Codina, A., Purser, L., Richardson, M., Müller–Quernheim, J., Greulich, T., Nowiński, A., Barta, I., Stendardo, M., et al. (2021) · The Lancet Microbe · doi:10.1016/s2666-5247(21)00035-5 · Cited by 137 · see citing works
Wu, B. G., Sulaiman, I., Tsay, J.-C. J., Perez, L., Franca, B., Li, Y., Wang, J., Gonzalez, A. N., El-Ashmawy, M., Carpenito, J., et al. (2020) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.202005-1596oc · Cited by 136 · see citing works
Dumas, A., Corral, D., Colom, A., Levillain, F., Peixoto, A., Hudrisier, D., Poquet, Y., & Neyrolles, O. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02656 · Cited by 130 · see citing works
Bacterial-Host Interactions: Physiology and Pathophysiology of Respiratory Infection
Håkansson, A., Orihuela, C. J., & Bogaert, D. (2018) · Physiological Reviews · doi:10.1152/physrev.00040.2016 · Cited by 126 · see citing works
Byrd, K. M., & Gulati, A. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.620124 · Cited by 118 · see citing works
Ran, Z., An, Y., Zhou, J., Yang, H. J., Zhang, Y., Yang, J., Wang, L., Li, X., Lu, D., Zhong, J. S., et al. (2020) · Environmental Pollution · doi:10.1016/j.envpol.2020.115987 · Cited by 121 · see citing works
Bacterial biogeography of adult airways in atopic asthma
Durack, J., Huang, Y. J., Nariya, S., Christian, L. S., Ansel, K. M., Beigelman, A., Castro, M., Dyer, A., Israel, E., Kraft, M., et al. (2018) · Microbiome · doi:10.1186/s40168-018-0487-3 · Cited by 117 · see citing works
Evaluation of the airway microbiome in nontuberculous mycobacteria disease
Sulaiman, I., Wu, B. G., Li, Y., Scott, A. S., Malecha, P. W., Scaglione, B. D., Wang, J., Basavaraj, A., Chung, S., Bantis, K., et al. (2018) · European Respiratory Journal · doi:10.1183/13993003.00810-2018 · Cited by 111 · see citing works
Yang, L., Haidar, G., Zia, H., Nettles, R., Qin, S., Wang, X., Shah, F. A., Rapport, S. F., Charalampous, T., Methé, B. A., et al. (2019) · Respiratory Research · doi:10.1186/s12931-019-1218-4 · Cited by 112 · see citing works
Kell, D. B., & Pretorius, E. (2018) · Biological reviews/Biological reviews of the Cambridge Philosophical Society · doi:10.1111/brv.12407 · Cited by 113 · see citing works
Su, Y.-C., Jalalvand, F., Thegerström, J., & Riesbeck, K. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02530 · Cited by 107 · see citing works
Pathophysiological role of respiratory dysbiosis in hospital-acquired pneumonia
Roquilly, A., Torres, A., Villadangos, J. A., Netea, M. G., Dickson, R. P., Becher, B., & Asehnoune, K. (2019) · The Lancet Respiratory Medicine · doi:10.1016/s2213-2600(19)30140-7 · Cited by 107 · see citing works
Self-citationThe microbiota in pneumonia: From protection to predisposition
Thibeault, C., Suttorp, N., & Opitz, B. (2021) · Science Translational Medicine · doi:10.1126/scitranslmed.aba0501 · Cited by 107 · see citing works
BTS clinical statement on aspiration pneumonia
Simpson, A. J., Allen, J.-L., Chatwin, M., Crawford, H., Elverson, J., Ewan, V. C., Forton, J. T., McMullan, R., Plevris, J. N., Renton, K., et al. (2023) · Thorax · doi:10.1136/thorax-2022-219699 · Cited by 104 · see citing works
Topography of the respiratory tract bacterial microbiota in cattle
McMullen, C., Alexander, T. W., Léguillette, R., Workentine, M. L., & Timsit, E. (2020) · Microbiome · doi:10.1186/s40168-020-00869-y · Cited by 102 · see citing works
Akkermansia muciniphila in neuropsychiatric disorders: friend or foe?
Lei, W., Cheng, Y., Gao, J., Liu, X., Shao, L., Kong, Q., Zheng, N., Ling, Z., & Hu, W. (2023) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2023.1224155 · Cited by 101 · see citing works
Durack, J., Christian, L. S., Nariya, S., Gonzalez, J. R., Bhakta, N. R., Ansel, K. M., Beigelman, A., Castro, M., Dyer, A., Israel, E., et al. (2020) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2020.03.028 · Cited by 97 · see citing works
Microbial dysbiosis and mortality during mechanical ventilation: a prospective observational study
for the PROSPECT Investigators, Lamarche, D., Canadian Critical Care Trials Group, Canadian Critical Care Translational Biology Group, Johnstone, J., Zytaruk, N. L., Clarke, F. J., Hand, L. E., Loukov, D., Szamosi, J. C., et al. (2018) · Respiratory Research · doi:10.1186/s12931-018-0950-5 · Cited by 95 · see citing works
Horn, K. J., Schopper, M. A., Drigot, Z., & Clark, S. E. (2022) · Nature Communications · doi:10.1038/s41467-022-31074-0 · Cited by 94 · see citing works
The Lung Microbiome and Its Role in Pneumonia
Wu, B. G., & Segal, L. N. (2018) · Clinics in Chest Medicine · doi:10.1016/j.ccm.2018.07.003 · Cited by 90 · see citing works
Kyo, M., Nishioka, K., Nakaya, T., Kida, Y., Tanabe, Y., Ohshimo, S., & Shime, N. (2019) · Respiratory Research · doi:10.1186/s12931-019-1203-y · Cited by 90 · see citing works
Healy, C. M., Muñoz‐Wolf, N., Strydom, J., Faherty, L., Williams, N. C., Kenny, S., Donnelly, S. C., & Cloonan, S. M. (2021) · Respiratory Research · doi:10.1186/s12931-021-01722-y · Cited by 89 · see citing works
Combs, M. P., Wheeler, D., Luth, J. E., Falkowski, N. R., Walker, N., Erb‐Downward, J. R., Lama, V. N., & Dickson, R. P. (2021) · The Lancet Respiratory Medicine · doi:10.1016/s2213-2600(20)30405-7 · Cited by 88 · see citing works
Self-citationTherapeutic Targeting of the Respiratory Microbiome
Chotirmall, S. H., Bogaert, D., Chalmers, J. D., Cox, M. J., Hansbro, P. M., Huang, Y. J., Molyneaux, P. L., O’Dwyer, D. N., Pragman, A. A., Rogers, G. B., et al. (2022) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.202112-2704pp · Cited by 88 · see citing works
Self-citationFilho, F. S. L., Takiguchi, H., Akata, K., Won, S. H., Moon, J.-Y., Kim, H. K., Cho, Y., Yamasaki, K., Milne, S. B., Yang, J., et al. (2021) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.202102-0289oc · Cited by 85 · see citing works
Robust airway microbiome signatures in acute respiratory failure and hospital-acquired pneumonia
Montassier, E., Kitsios, G. D., Radder, J. E., Bastard, Q. L., Kelly, B. J., Panzer, A. R., Lynch, S. V., Calfee, C. S., Dickson, R. P., & Roquilly, A. (2023) · Nature Medicine · doi:10.1038/s41591-023-02617-9 · Cited by 85 · see citing works
Self-citationNeisseria species as pathobionts in bronchiectasis
Li, L., Aogáin, M. M., Xu, T., Jaggi, T. K., Chan, L., Qu, J., Wei, L., Liao, S., Cheng, H. S., Keir, H. R., et al. (2022) · Cell Host & Microbe · doi:10.1016/j.chom.2022.08.005 · Cited by 81 · see citing works
Kitsios, G. D., Fitch, A. C., Manatakis, D. V., Rapport, S. F., Li, K., Qin, S., Huwe, J., Zhang, Y., Doi, Y., Evankovich, J. W., et al. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.01413 · Cited by 80 · see citing works
Nasopharyngeal Microbial Communities of Patients Infected With SARS-CoV-2 That Developed COVID-19
Ventero, M. P., Cuadrat, R. R. C., Vidal, I., Andrade, B. G. N., Molina-Pardines, C., Haro-Moreno, J. M., Coutinho, F. H., Merino, E., Regitano, L. C. D. A., Silveira, C. B., et al. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.637430 · Cited by 80 · see citing works
Facciponte, D. N., Bough, M. W., Seidler, D., Carroll, J. L., Ashare, A., Andrew, A. S., Tsongalis, G. J., Vaickus, L. J., Henegan, P. L., Butt, T. H., et al. (2018) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2018.07.226 · Cited by 79 · see citing works
The importance of airway and lung microbiome in the critically ill
Martín‐Loeches, I., Dickson, R. P., Torres, A., Hanberger, H., Lipman, J., Antonelli, M. M., PASCALE, G. D., Bozza, F. A., Vincent, J., Murthy, S., et al. (2020) · Critical Care · doi:10.1186/s13054-020-03219-4 · Cited by 79 · see citing works
Self-citationThe human lung microbiome—A hidden link between microbes and human health and diseases
Yi, X., Gao, J., & Wang, Z. (2022) · iMeta · doi:10.1002/imt2.33 · Cited by 77 · see citing works
Anterior Nares Diversity and Pathobionts Represent Sinus Microbiome in Chronic Rhinosinusitis
Boeck, I. D., Wittouck, S., Martens, K., Claes, J., Jorissen, M., Steelant, B., van den Broek, M. F. L., Seys, S., Hellings, P. W., Vanderveken, O. M., et al. (2019) · mSphere · doi:10.1128/msphere.00532-19 · Cited by 75 · see citing works
Role of Gut Microbiome in COVID-19: An Insight Into Pathogenesis and Therapeutic Potential
Hussain, I., Cher, G. L. Y., Abid, M. A., & Abid, M. B. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.765965 · Cited by 72 · see citing works
Role of Lung Microbiome in Innate Immune Response Associated With Chronic Lung Diseases
Paudel, K. R., Dharwal, V., Patel, V. K., Galvão, I., Wadhwa, R., Malyla, V., Shen, S., Budden, K. F., Hansbro, N. G., Vaughan, A., et al. (2020) · Frontiers in Medicine · doi:10.3389/fmed.2020.00554 · Cited by 74 · see citing works
Jorth, P. A., Ehsan, Z., Rezayat, A., Caldwell, E., Pope, C. E., Brewington, J. J., Goss, C. H., Benscoter, D. T., Clancy, J. P., & Singh, P. K. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.03.086 · Cited by 72 · see citing works
Invernizzi, R., Barnett, J. L., Rawal, B., Nair, A., Ghai, P., Kingston, S., Chua, F., Wu, Z., Wells, A. U., Renzoni, E., et al. (2020) · European Respiratory Journal · doi:10.1183/13993003.01519-2019 · Cited by 72 · see citing works
Respiratory microbiome in mechanically ventilated patients: a narrative review
Fromentin, M., Ricard, J.-D., & Roux, D. (2021) · Intensive Care Medicine · doi:10.1007/s00134-020-06338-2 · Cited by 72 · see citing works
Das, S., Bernasconi, E., Koutsokera, A., Wurlod, D.-A., Tripathi, V., Bonilla‐Rosso, G., Aubert, J., Derkenne, M.-F., Mercier, L., Pattaroni, C., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-22344-4 · Cited by 72 · see citing works
The Lung Microbiome and Pneumonia
Pettigrew, M. M., Tanner, W. D., & Harris, A. D. (2020) · The Journal of Infectious Diseases · doi:10.1093/infdis/jiaa702 · Cited by 70 · see citing works
More of the Gut in the Lung: How Two Microbiomes Meet in ARDS.
Mukherjee, S., & Hanidziar, D. (2018) · Europe PMC (PubMed Central) · Cited by 66 · see citing works
Microbiota and Immunity during Respiratory Infections: Lung and Gut Affair
Marrella, V., Nicchiotti, F., & Cassani, B. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25074051 · Cited by 65 · see citing works
Understanding respiratory microbiome–immune system interactions in health and disease
Simone, S. K. D., Rudloff, I., Nold‐Petry, C. A., Forster, S. C., & Nold, M. F. (2023) · Science Translational Medicine · doi:10.1126/scitranslmed.abq5126 · Cited by 61 · see citing works
Past, Present, and Future Research on the Lung Microbiome in Inflammatory Airway Disease
Caverly, L. J., Huang, Y. J., & Sze, M. A. (2019) · CHEST Journal · doi:10.1016/j.chest.2019.05.011 · Cited by 61 · see citing works
Hérivaux, A., Willis, J. R., Mercier, T., Lagrou, K., Gonçalves, S. M., Gonçales, R. A., Maertens, J. A., Carvalho, A., Gabaldón, T., & Cunha, C. (2021) · Thorax · doi:10.1136/thoraxjnl-2020-216179 · Cited by 61 · see citing works
The Lung Microbiome in Idiopathic Pulmonary Fibrosis: A Promising Approach for Targeted Therapies
Fastrès, A., Felice, F., Roels, É., Moermans, C., Corhay, J.-L., Bureau, F., Louis, R. E., Clercx, C., & Guiot, J. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18122735 · Cited by 59 · see citing works
Effects of smoking on the lower respiratory tract microbiome in mice
Zhang, R., Chen, L., Cao, L., Li, K., Huang, Y. H., Luan, X.-Q., & Li, G. (2018) · Respiratory Research · doi:10.1186/s12931-018-0959-9 · Cited by 59 · see citing works
Langelier, C., Kalantar, K., Moazed, F., Wilson, M. R., Crawford, E. D., Deiss, T. J., Belzer, A., Bolourchi, S., Caldera, S., Fung, M., et al. (2019) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00476.2018 · Cited by 59 · see citing works
Microbiome dysbiosis and epigenetic modulations in lung cancer: From pathogenesis to therapy
Khan, F. H., Bhat, B. A., Sheikh, B. A., Tariq, L., Padmanabhan, R., Verma, J. P., Shukla, A. C., Dowlati, A., & Abbas, A. (2021) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2021.07.005 · Cited by 59 · see citing works
Laboratory contamination in airway microbiome studies
Drengenes, C., Wiker, H. G., Kalananthan, T., Nordeide, E., Eagan, T., & Nielsen, R. (2019) · BMC Microbiology · doi:10.1186/s12866-019-1560-1 · Cited by 58 · see citing works
Phage therapy for pulmonary infections: lessons from clinical experiences and key considerations
Mitropoulou, G., Koutsokera, A., Csajka, C., Blanchon, S., Sauty, A., Brunet, J.-F., von Garnier, C., Resch, G., & Guery, B. P. (2022) · European Respiratory Review · doi:10.1183/16000617.0121-2022 · Cited by 55 · see citing works
The Lung Microbiome: A New Frontier for Lung and Brain Disease
Chen, J., Li, T., Ye, C., Zhong, J., Huang, J., Ke, Y., & Sun, H. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24032170 · Cited by 55 · see citing works
Wu, X., Chen, J., Xu, M., Zhu, D., Wang, X., Chen, Y., Wu, J., Cui, C., Zhang, W., & Yu, L. (2017) · Journal of Oral Microbiology · doi:10.1080/20002297.2017.1324725 · Cited by 55 · see citing works
Critical Relevance of Stochastic Effects on Low-Bacterial-Biomass 16S rRNA Gene Analysis
Erb‐Downward, J. R., Falkowski, N. R., D’Souza, J. C., McCloskey, L., McDonald, R. A., Brown, C. A., Shedden, K., Dickson, R. P., Freeman, C. M., Stringer, K. A., et al. (2020) · mBio · doi:10.1128/mbio.00258-20 · Cited by 55 · see citing works
Self-citationThe porcine respiratory microbiome: recent insights and future challenges
Pirolo, M., Espinosa‐Gongora, C., Bogaert, D., & Guardabassi, L. (2021) · Animal Microbiome · doi:10.1186/s42523-020-00070-4 · Cited by 54 · see citing works
Pulvirenti, G., Parisi, G. F., Giallongo, A., Papale, M. R., Manti, S., Savasta, S., Licari, A., Marseglia, G. L., & Leonardi, S. (2019) · Frontiers in Pediatrics · doi:10.3389/fped.2019.00393 · Cited by 52 · see citing works
Baker, J. M., Hinkle, K. J., McDonald, R. A., Brown, C. A., Falkowski, N. R., Huffnagle, G. B., & Dickson, R. P. (2021) · Microbiome · doi:10.1186/s40168-021-01055-4 · Cited by 52 · see citing works
Self-citationDifferent responses of the oral, nasal and lung microbiomes to cigarette smoke
Pfeiffer, S., Herzmann, C., Gaede, K. I., Kovacevic, D., Krauss‐Etschmann, S., & Schloter, M. (2021) · Thorax · doi:10.1136/thoraxjnl-2020-216153 · Cited by 52 · see citing works
Kitsios, G. D., Sayed, K., Fitch, A. C., Yang, H., Britton, N., Shah, F. A., Bain, W., Evankovich, J. W., Qin, S., Wang, X., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-48819-8 · Cited by 52 · see citing works
Invernizzi, R., & Molyneaux, P. L. (2019) · European Respiratory Review · doi:10.1183/16000617.0045-2019 · Cited by 53 · see citing works
Sputum microbiome profiling in COPD: beyond singular pathogen detection
Ditz, B., Christenson, S. A., Rossen, J. W. A., Brightling, C. E., Kerstjens, H. A. M., van den Berge, M., & Faiz, A. (2020) · Thorax · doi:10.1136/thoraxjnl-2019-214168 · Cited by 52 · see citing works
The chemistry of gut microbiome in health and diseases
Lau, A., Tan, L. T., Mutalib, N. A., Wong, S. H., Letchumanan, V., & Lee, L. (2021) · Progress In Microbes & Molecular Biology · doi:10.36877/pmmb.a0000175 · Cited by 52 · see citing works
The lung microbiome in lung transplantation
McGinniss, J. E., Whiteside, S. A., Simón‐Soro, Á., Diamond, J. M., Christie, J. D., Bushman, F. D., & Collman, R. G. (2021) · The Journal of Heart and Lung Transplantation · doi:10.1016/j.healun.2021.04.014 · Cited by 52 · see citing works
Immunomodulatory Properties of Vitamin D in the Intestinal and Respiratory Systems
Hamza, F. N., Daher, S., Fakhoury, H. M. A., Grant, W. B., Kvietys, P. R., & AlKattan, K. M. (2023) · Nutrients · doi:10.3390/nu15071696 · Cited by 51 · see citing works
Microbiome in interstitial lung disease
Salisbury, M. L., Han, M. K., Dickson, R. P., & Molyneaux, P. L. (2017) · Current Opinion in Pulmonary Medicine · doi:10.1097/mcp.0000000000000399 · Cited by 51 · see citing works
Self-citationHost-microbe interactions in the pathogenesis and clinical course of sarcoidosis
Inaoka, P. T., Shono, M., Kamada, M., & Espinoza, J. L. (2019) · Journal of Biomedical Science · doi:10.1186/s12929-019-0537-6 · Cited by 51 · see citing works
- Authors
- Dickson, R. P., Erb-Downward, J. R., Freeman, C. M., McCloskey, L., Falkowski, N. R., Huffnagle, G. B., & Curtis, J. L.
- Affiliations
- Michigan Medicine (US)
- Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Health System, Ann Arbor, Michigan, USA
- VA Ann Arbor Healthcare System (US)
- University of Michigan (US)
- Corresponding author
- Curtis, Jeffrey L.
- Venue
- mBio
- Publisher
- American Society for Microbiology
- Published
- 2017-03-08
- Volume
- 8
- Issue
- 1
- ISSN
- 2150-7511 (ISSN-L)
- 2161-2129
- PMC
- PMC5312084
- OpenAlex
- W2588100341
- Cited by
- 548
- References
- 45
- Citations by year
- 2026: 46 · 2025: 60 · 2024: 101 · 2023: 48 · 2022: 68 · 2021: 64 · 2020: 55 · 2019: 40 · 2018: 48 · 2017: 18
- FWCI
- 17.99
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- mBio (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- HHS | National Institutes of Health (UL1 TR000433, K23 HL130641, U01 HL098961, R01 HL114447)
- U.S. Department of Veterans Affairs (I01 BX001389, I01 CX000911)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Medicine › Critical Care and Intensive Care Medicine › Nosocomial Infections in ICU
- Keywords
- lung microbiome, lower respiratory tract, bronchoscopic sampling, bronchoalveolar lavage, microaspiration, 16S rRNA gene sequencing
- MeSH terms
- Adult, Aged, Bacteria, Bronchoalveolar Lavage Fluid, Bronchoscopy, DNA, Bacterial, Female, Humans, Lung, Male, Middle Aged, Mouth, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Bronchoalveolar Lavage, Metagenome, Microbiota, Healthy Volunteers
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