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Cited by 1,138

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Autophosphorylation of the Focal Adhesion Kinase, ppl25FAK, Directs SH2-Dependent Binding of pp60src

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Schaller, Michael D., Hildebrand, Jeffrey D., Shannon, John D., Fox, Jay W., Vines, Richard R., Parsons, J. Thomas·1994·Molecular and Cellular Biology
DOI 10.1128/mcb.14.3.1680-1688.1994
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Abstract

The phosphorylation of protein tyrosine kinases (PTKs) on tyrosine residues is a critical regulatory event that modulates catalytic activity and triggers the physical association of PTKs with Src homology 2 (SH2)-containing proteins. The integrin-linked focal adhesion kinase, pp125FAK, exhibits extracellular matrix-dependent phosphorylation on tyrosine and physically associates with two nonreceptor PTKs, pp60src and pp59fyn, via their SH2 domains. Herein, we identify Tyr-397 as the major site of tyrosine phosphorylation on pp125FAK both in vivo and in vitro. Tyrosine 397 is located at the juncture of the N-terminal and catalytic domains, a novel site for PTK autophosphorylation. Mutation of Tyr-397 to a nonphosphorylatable residue dramatically impairs the phosphorylation of pp125FAK on tyrosine in vivo and in vitro. The mutation of Tyr-397 to Phe also inhibits the formation of stable complexes with pp60src in cells expressing Src and FAK397F, suggesting that autophosphorylation of pp125FAK may regulate the association of pp125FAK with Src family kinases in vivo. The identification of Tyr-397 as a major site for FAK autophosphorylation provides one of the first examples of a cellular protein containing a high-affinity binding site for a Src family kinase SH2 domain. This finding has implications for models describing the mechanisms of action of pp125FAK, the regulation of the Src family of PTKs, and signal transduction through the integrins.

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Research works printed but not linked: 59

  1. Aebersold R., 1990, Anal. Biochem., 187, 56, 10.1016/0003-2697(90)90417-8 · DOI
  2. 10.1002/j.1460-2075.1992.tb05426.x · DOI · Paper page
  3. 10.1016/0076-6879(91)01013-R · DOI · Paper page
  4. 10.1083/jcb.119.4.893 · DOI · Paper page
  5. 10.1016/0092-8674(91)90639-G · DOI · Paper page
  6. 10.1016/S0021-9258(18)98375-4 · DOI · Paper page
  7. 10.1128/mcb.14.1.147-155.1994 · DOI
  8. 10.1016/0092-8674(93)90634-3 · DOI
  9. 10.1126/science.2466336 · DOI
  10. Coull J. M., 1991, Anal. Biochem., 194, 110, 10.1016/0003-2697(91)90157-O · DOI
  11. 10.1038/362087a0 · DOI · Paper page
  12. 10.1016/0092-8674(92)90444-H · DOI
  13. 10.1126/science.1848725 · DOI · Paper page
  14. 10.1038/358690a0 · DOI
  15. 10.1091/mbc.2.11.951 · DOI · Paper page
  16. 10.1073/pnas.89.18.8487 · DOI · Paper page
  17. 10.1083/jcb.123.4.993 · DOI · Paper page
  18. 10.1128/jvi.61.10.3004-3012.1987 · DOI
  19. 10.1016/0092-8674(92)90115-S · DOI · Paper page
  20. 10.1083/jcb.120.3.577 · DOI · Paper page
  21. Kanner S. B., 1989, Methods, 120, 115
  22. 10.1073/pnas.87.9.3328 · DOI
  23. 10.1016/0092-8674(91)90461-7 · DOI · Paper page
  24. 10.1016/S0021-9258(18)35853-8 · DOI · Paper page
  25. 10.1073/pnas.88.19.8392 · DOI · Paper page
  26. 10.1073/pnas.86.21.8232 · DOI
  27. Lau A. F., 1986, Virology, 151, 86, 10.1016/0042-6822(86)90106-6 · DOI
  28. 10.1083/jcb.119.4.905 · DOI · Paper page
  29. Liu X., 1993, Oncogene, 8, 1119
  30. 10.1016/0076-6879(91)01014-S · DOI
  31. 10.1126/science.1694307 · DOI · Paper page
  32. Mayer B. J., 1990, Proc. Natl. Acad. Sci. USA, 86, 2638, 10.1073/pnas.87.7.2638 · DOI
  33. 10.1038/363015a0 · DOI · Paper page
  34. Meyers M. G., 1992, Proc. Natl. Acad. Sci. USA, 89, 10350, 10.1073/pnas.89.21.10350 · DOI
  35. 10.1002/j.1460-2075.1993.tb05879.x · DOI
  36. Morrison D. K., 1990, Mol. Cell. Biol., 10, 2359
  37. 10.1038/351069a0 · DOI · Paper page
  38. Naldini L., 1991, Mol. Cell. Biol., 11, 1793
  39. 10.1126/science.1700866 · DOI
  40. 10.1002/j.1460-2075.1992.tb05524.x · DOI
  41. 10.1128/jvi.51.2.272-282.1984 · DOI
  42. 10.1016/0092-8674(92)90504-6 · DOI · Paper page
  43. 10.1016/0960-9822(93)90350-W · DOI · Paper page
  44. 10.1128/MCB.9.9.3951 · DOI
  45. 10.1128/MCB.9.2.629 · DOI · Paper page
  46. 10.1073/pnas.80.11.3237 · DOI
  47. 10.1073/pnas.89.11.5192 · DOI · Paper page
  48. 10.1128/MCB.13.2.785 · DOI · Paper page
  49. 10.1016/0962-8924(93)90053-4 · DOI
  50. 10.1016/0896-6273(92)90177-F · DOI · Paper page
  51. 10.1016/0962-8924(92)90120-C · DOI
  52. 10.1016/0955-0674(91)90062-4 · DOI
  53. 10.1016/0092-8674(93)90404-E · DOI · Paper page
  54. 10.1002/j.1460-2075.1992.tb05492.x · DOI · Paper page
  55. 10.1016/0092-8674(90)90801-K · DOI · Paper page
  56. Waksman G., 1993, Cell, 72, 770, 10.1016/0092-8674(93)90405-F · DOI
  57. 10.1016/0092-8674(93)90221-B · DOI
  58. 10.1016/S0021-9258(17)42986-3 · DOI
  59. 10.1016/0092-8674(92)90385-P · DOI · Paper page

Cited by

Metadata

Authors
Schaller, M. D., Hildebrand, J. D., Shannon, J. D., Fox, J. W., Vines, R. R., & Parsons, J. T.
Venue
Molecular and Cellular Biology
Publisher
Informa UK Limited
Published
1994-03-01
Volume
14
Issue
3
Pages
1680-1688
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
DOI
10.1128/mcb.14.3.1680-1688.1994
OpenAlex
W1785533687
Cited by
1,138
References
1
FWCI
16.92
Type
journal article
Language
en
Open access
green
Licence
journals.asm.org/non-commercial-tdm-license (tdm)

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