The APO(*)E3-Leiden mouse as an animal model for basal laminar deposit
Open accessAbstract
AIM: To investigate the APO(*)E3-Leiden mouse as an animal model for age related maculopathy (ARM) related extracellular deposits. METHODS: Eyes were obtained from APO(*)E3-Leiden transgenic mice on a high fat/cholesterol (HFC) diet (n=12) or on a normal mouse chow (n=6), for 9 months. As controls, eyes were collected from APO-E knockout mice on the same diets. From each mouse one eye was processed for microscopic evaluation and immunohistochemistry with a polyclonal antibody directed against human apo-E. Electron microscopy was also performed. RESULTS: All 12 eyes of the APO(*)E3-Leiden mice on an HFC diet contained basal laminar deposit (BLD; class 1 to class 3), whereas two of six APO(*)E3-Leiden mice on normal chow showed BLD class 1. The ultrastructural aspects of this BLD were comparable with those seen in early BLD in humans, and BLD showed immunoreaction with anti-human-apo-E antibodies. No BLD was found in any of the control mice. Drusen were not detected in any of the mice. CONCLUSION: These results indicate that APO(*)E3-Leiden mice can be used as animal model for the pathogenesis of BLD, and that a HFC diet enhances the accumulation of this deposit. Furthermore, this study supports the previously suggested involvement of dysfunctional apo-E in the accumulation of extracellular deposits in ARM.
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After 9 months on a high fat/cholesterol diet, 100% of APO*E3 Leiden mice eyes have BlamD, while 33% of APO*E3 Leiden mice on a normal diet have BlamD.
“After 9 months on a high fat/cholesterol diet, 100% of APO*E3 Leiden mice eyes have BlamD, while 33% of APO*E3 Leiden mice on a normal diet have BlamD (Kliffen et al., 2000).”
From Retinal ultrastructure of murine models of dry age-related macular degeneration (AMD) (2010) · cites this paper as Kliffen et al., 2000
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- Authors
- Kliffen, M.
- Affiliations
- Erasmus MC (NL)
- Corresponding author
- Kliffen, M.
- Venue
- British Journal of Ophthalmology
- Publisher
- BMJ
- Published
- 2000-12-01
- Volume
- 84
- Issue
- 12
- Pages
- 1415-1419
- ISSN
- 0007-1161 (ISSN-L)
- PMC
- PMC1723360
- OpenAlex
- W2165816509
- Cited by
- 71
- References
- 43
- Citations by year
- 2025: 2 · 2024: 1 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 2 · 2015: 3 · 2014: 4 · 2013: 5 · 2012: 4
- FWCI
- 1.90
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- British Journal of Ophthalmology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (publishedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion, other-oa)
- RePub (Erasmus University Rotterdam) (submittedVersion, other-oa)
- Pure Amsterdam UMC (submittedVersion)
- KNAW Research Portal (The Royal Netherlands Academy of Arts and Sciences) (submittedVersion)
- RePub (Erasmus University Rotterdam) (submittedVersion)
- Topics
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Keywords
- basal laminar deposits, age-related maculopathy, animal models, high-fat high-cholesterol diet, apolipoprotein E, drusen, retinal ultrastructure, ApoE-deficient mice
- MeSH terms
- Animals, Apolipoproteins E, Basement Membrane, Dietary Fats, Disease Models, Animal, Humans, Immunoenzyme Techniques, Macular Degeneration, Mice, Transgenic, Retina, Mice, Apolipoprotein E3
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