CoChat
Sign Up
Cited by 32Muscle metabolism and nutritionMetabolism, Diabetes, and CancerGlycogen Storage Diseases and Myoclonus

Increases in glycogenin and glycogenin mRNA accompany glycogen resynthesis in human skeletal muscle

Shearer, Jane, Wilson, Rhonda J., Battram, Danielle S., Richter, Erik A., Robinson, Deborah L., Bakovic, Marica, Graham, Terry E.·2005·American Journal of Physiology-Endocrinology and Metabolism
DOI 10.1152/ajpendo.00100.2005
Open this paper in CoChat — free account

Abstract

Glycogenin is the self-glycosylating protein primer that initiates glycogen granule formation. To examine the role of this protein during glycogen resynthesis, eight male subjects exercised to exhaustion on a cycle ergometer at 75% V̇o 2 max followed by five 30-s sprints at maximal capacity to further deplete glycogen stores. During recovery, carbohydrate (75 g/h) was supplied to promote rapid glycogen repletion, and muscle biopsies were obtained from the vastus lateralis at 0, 30, 120, and 300 min postexercise. At time 0, no free (deglycosylated) glycogenin was detected in muscle, indicating that all glycogenin was complexed to carbohydrate. Glycogenin activity, a measure of the glycosylating ability of the protein, increased at 30 min and remained elevated for the remainder of the study. Quantitative RT-PCR showed elevated glycogenin mRNA at 120 min followed by increases in protein levels at 300 min. Glycogenin specific activity (glycogenin activity/relative protein content) was also elevated at 120 min. Proglycogen increased at all time points, with the highest rate of resynthesis occurring between 0 and 30 min. In comparison, macroglycogen levels did not significantly increase until 300 min postexercise. Together, these results show that, during recovery from prolonged exhaustive exercise, glycogenin mRNA and protein content and activity increase in muscle. This may facilitate rapid glycogen resynthesis by providing the glycogenin backbone of proglycogen, the major component of glycogen synthesized in early recovery.

Integrity signals

Registry

Crossref · 10.1152/ajpendo.00100.2005

Cites

36 of 36 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • A modified glycogen loading protocol of high carbohydrate intake and exercise taper, deleting the depletion phase, was similarly successful in athletes in the study by Sherman and colleagues.

    “A 'modified' protocol of high CHO intake and exercise taper, deleting the depletion phase, was found to be similarly successful in athletes in the latter study (96).”

    From Postexercise muscle glycogen resynthesis in humans. (2017) · cites this paper as [96]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Shearer, J., Wilson, R. J., Battram, D. S., Richter, E. A., Robinson, D. L., Bakovic, M., & Graham, T. E.
Affiliations
University of Guelph (CA)
ORCID
Venue
American Journal of Physiology-Endocrinology and Metabolism
Publisher
American Physiological Society
Published
2005-09
Volume
289
Issue
3
Pages
E508-E514
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
DOI
10.1152/ajpendo.00100.2005
OpenAlex
W2151408372
Cited by
32
References
36
Citations by year
2023: 1 · 2021: 1 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 5
FWCI
1.22
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
Funders
University of Guelph
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Rheumatology › Glycogen Storage Diseases and Myoclonus
Keywords
glycogenin, glycogen resynthesis, skeletal muscle, carbohydrate supplementation, exhaustive exercise, post-exercise recovery, vastus lateralis, glycogen synthesis
MeSH terms
Adult, Blood Glucose, Exercise Test, Glucosyltransferases, Glycogen, Glycoproteins, Humans, Insulin, Male, Oxygen Consumption, RNA, Messenger, Transcription, Genetic, Gene Expression, Muscle, Skeletal

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review