Cited by 489Muscle Physiology and DisordersNutrition and Health in AgingMuscle metabolism and nutrition

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Satellite cell content is specifically reduced in type II skeletal muscle fibers in the elderly

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Verdijk, Lex B., Koopman, René, Schaart, Gert, Meijer, Kenneth, Savelberg, Hans H. C. M., van Loon, Luc J. C.·2007·American Journal of Physiology-Endocrinology and Metabolism

Abstract

Satellite cells (SC) are essential for skeletal muscle growth and repair. Because sarcopenia is associated with type II muscle fiber atrophy, we hypothesized that SC content is specifically reduced in the type II fibers in the elderly. A total of eight elderly (E; 76 ± 1 yr) and eight young (Y; 20 ± 1 yr) healthy males were selected. Muscle biopsies were collected from the vastus lateralis in both legs. ATPase staining and a pax7-antibody were used to determine fiber type-specific SC content (i.e., pax7-positive SC) on serial muscle cross sections. In contrast to the type I fibers, the proportion and mean cross-sectional area of the type II fibers were substantially reduced in E vs. Y. The number of SC per type I fiber was similar in E and Y. However, the number of SC per type II fiber was substantially lower in E vs. Y (0.044 ± 0.003 vs. 0.080 ± 0.007; P < 0.01). In addition, in the type II fibers, the number of SC relative to the total number of nuclei and the number of SC per fiber area were also significantly lower in E. This study is the first to show type II fiber atrophy in the elderly to be associated with a fiber type-specific decline in SC content. The latter is evident when SC content is expressed per fiber or per fiber area. The decline in SC content might be an important factor in the etiology of type II muscle fiber atrophy, which accompanies the loss of skeletal muscle with aging.

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Crossref · 10.1152/ajpendo.00278.2006

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Metadata

Authors
Verdijk, L. B., Koopman, R., Schaart, G., Meijer, K., Savelberg, H. H. C. M., & van Loon, L. J. C.
Affiliations
Maastricht University (NL)
Venue
American Journal of Physiology-Endocrinology and Metabolism
Published
2007-01
Volume
292
Issue
1
Pages
E151-E157
ISSN
  • 0193-1849 (ISSN-L)
  • 1522-1555
OpenAlex
W2113612408
Cited by
489
References
51
Citations by year
2026: 14 · 2025: 17 · 2024: 21 · 2023: 20 · 2022: 35 · 2021: 33 · 2020: 27 · 2019: 27 · 2018: 35 · 2017: 25 · 2016: 28 · 2015: 28 · 2014: 35 · 2013: 36 · 2012: 27
FWCI
4.48
Type
journal article
Language
en
Licence
other-oa
Locations
  • American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (submittedVersion, other-oa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Physiology (medical) › Nutrition and Health in Aging
  • Health Sciences › Nursing › Nutrition and Dietetics › Muscle metabolism and nutrition
Keywords
satellite cells, sarcopenia, muscle atrophy, aging, older adults, Pax7, vastus lateralis, muscle fiber cross-sectional area
MeSH terms
Adult, Aged, Atrophy, Biopsy, Cell Count, Cell Nucleus, Humans, Male, Muscle Fibers, Fast-Twitch, Satellite Cells, Skeletal Muscle

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