The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Proteomic identification of secreted proteins from human skeletal muscle cells and expression in response to strength training
Abstract
Regular physical activity protects against several types of diseases. This may involve altered secretion of signaling proteins from skeletal muscle. Our aim was to identify the most abundantly secreted proteins in cultures of human skeletal muscle cells and to monitor their expression in muscles of strength-training individuals. A total of 236 proteins were detected by proteome analysis in medium conditioned by cultured human myotubes, which was narrowed down to identification of 18 classically secreted proteins expressed in skeletal muscle, using the SignalP 3.0 and Human Genome Expression Profile databases together with a published mRNA-based reconstruction of the human skeletal muscle secretome. For 17 of the secreted proteins, expression was confirmed at the mRNA level in cultured human myotubes as well as in biopsies of human skeletal muscles. RT-PCR analyses showed that 15 of the secreted muscle proteins had significantly enhanced mRNA expression in m. vastus lateralis and/or m. trapezius after 11 wk of strength training among healthy volunteers. For example, secreted protein acidic and rich in cysteine, a secretory protein in the membrane fraction of skeletal muscle fibers, was increased 3- and 10-fold in m. vastus lateralis and m. trapezius, respectively. Identification of proteins secreted by skeletal muscle cells in vitro facilitated the discovery of novel responses in skeletal muscles of strength-training individuals.
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Cites
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“Whereas endurance training typically improves cardiovascular function (31), strength training reduces sarcopenia (44).”
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MethodInfluentialIL-7 is expressed and secreted by human skeletal muscle cells
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Self-citation
Cited by
Muscles, exercise and obesity: skeletal muscle as a secretory organ
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Metabolic Syndrome and Insulin Resistance: Underlying Causes and Modification by Exercise Training
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Exercise benefits in cardiovascular disease: beyond attenuation of traditional risk factors
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Exercise is the real polypill.
Fiuza-Luces, C., Garatachea, N., Berger, N. A., & Lucia, A. (2013) · Physiology · doi:10.1152/physiol.00019.2013 · Cited by 791
Pedersen, B. K. (2013) · Comprehensive physiology · doi:10.1002/cphy.c120033 · Cited by 516
Metadata
- Authors
- Norheim, F., Raastad, T., Thiede, B., Rustan, A. C., Drevon, C. A., & Haugen, F.
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2011-11
- Volume
- 301
- Issue
- 5
- Pages
- E1013-E1021
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2114129845
- Cited by
- 181
- References
- 10
- FWCI
- 5.17
- Type
- journal article
- Language
- en
- Open access
- closed
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