The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Calorie restriction increases fatty acid synthesis and whole body fat oxidation rates
Open accessAbstract
Calorie restriction (CR) increases longevity and retards the development of many chronic diseases, but the underlying metabolic signals are poorly understood. Increased fatty acid (FA) oxidation and reduced FA synthesis have been hypothesized to be important metabolic adaptations to CR. However, at metabolic steady state, FA oxidation must match FA intake plus synthesis; moreover, FA intake is low, not high, during CR. Therefore, it is not clear how FA dynamics are altered during CR. Accordingly, we measured food intake patterns, whole body fuel selection, endogenous FA synthesis, and gene expression in mice on CR. Within 2 days of CR being started, a shift to a cyclic, diurnal pattern of whole body FA metabolism occurred, with an initial phase of elevated endogenous FA synthesis [respiratory exchange ratio (RER) >1.10, lasting 4–6 h after food provision], followed by a prolonged phase of FA oxidation (RER = 0.70, lasting 18–20 h). CR mice oxidized four times as much fat per day as ad libitum (AL)-fed controls (367 ± 19 vs. 97 ± 14 mg/day, P < O.001) despite reduced energy intake from fat. This increase in FA oxidation was balanced by a threefold increase in adipose tissue FA synthesis compared with AL. Expression of FA synthase and acetyl-CoA carboxylase mRNA were increased in adipose and liver in a time-dependent manner. We conclude that CR induces a surprising metabolic pattern characterized by periods of elevated FA synthesis alternating with periods of FA oxidation disproportionate to dietary FA intake. This pattern may have implications for oxidative damage and disease risk.
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A Ketogenic Diet Extends Longevity and Healthspan in Adult Mice
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“…profiles showed an enhancement in mitochondrial respiration driven by fatty acid degradation, which was accompanied by increased expression of respiratory complexes and which is expected from greater use of fat as fuel source (Bruss et al., 2010) and concomitant alteration in body fat composition.”
Background
Metadata
- Authors
- Bruss, M. D., Khambatta, C. F., Ruby, M. A., Aggarwal, I., & Hellerstein, M. K.
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2010-01
- Volume
- 298
- Issue
- 1
- Pages
- E108-E116
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- OpenAlex
- W2107874132
- Cited by
- 320
- References
- 45
- FWCI
- 5.90
- Type
- journal article
- Language
- en
- Open access
- green
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- Bruss, M. D., Khambatta, C. F., Ruby, M. A., Aggarwal, I., & Hellerstein, M. K. (2010). Calorie restriction increases fatty acid synthesis and whole body fat oxidation rates. American Journal of Physiology-Endocrinology and Metabolism. https://doi.org/10.1152/ajpendo.00524.2009
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