Cited by 516Diet and metabolism studiesGenetics, Aging, and Longevity in Model OrganismsDietary Effects on Health

A Ketogenic Diet Extends Longevity and Healthspan in Adult Mice

Open access
Roberts, Megan N., Wallace, Marita A., Tomilov, Alexey A., Zhou, Zeyu, Marcotte, George R., Tran, Dianna, Perez, Gabriella, Gutierrez-Casado, Elena +12 more·2017·Cell Metabolism

Abstract

Calorie restriction, without malnutrition, has been shown to increase lifespan and is associated with a shift away from glycolysis toward beta-oxidation. The objective of this study was to mimic this metabolic shift using low-carbohydrate diets and to determine the influence of these diets on longevity and healthspan in mice. C57BL/6 mice were assigned to a ketogenic, low-carbohydrate, or control diet at 12 months of age and were either allowed to live their natural lifespan or tested for physiological function after 1 or 14 months of dietary intervention. The ketogenic diet (KD) significantly increased median lifespan and survival compared to controls. In aged mice, only those consuming a KD displayed preservation of physiological function. The KD increased protein acetylation levels and regulated mTORC1 signaling in a tissue-dependent manner. This study demonstrates that a KD extends longevity and healthspan in mice.

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Crossref · 10.1016/j.cmet.2017.08.005

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Authors
Roberts, M. N., Wallace, M. A., Tomilov, A. A., Zhou, Z., Marcotte, G. R., Tran, D., Perez, G., Gutierrez-Casado, E., Koike, S., Knotts, T. A., Imai, D. M., Griffey, S. M., Kim, K., Hagopian, K., McMackin, M. Z., Haj, F. G., Baar, K., Cortopassi, G. A., Ramsey, J. J., & Lopez-Dominguez, J. A.
Affiliations
University of California, Davis (US)
Corresponding author
  • Ramsey, Jon J.
  • Lopez-Dominguez, Jose Alberto
Venue
Cell Metabolism
Publisher
Elsevier BV
Published
2017-09
Volume
26
Issue
3
Pages
539-546.e5
ISSN
1550-4131 (ISSN-L)
OpenAlex
W2750582921
Cited by
514
References
34
Citations by year
2026: 33 · 2025: 63 · 2024: 67 · 2023: 68 · 2022: 67 · 2021: 66 · 2020: 65 · 2019: 43 · 2018: 32 · 2017: 7 · 2015: 1
FWCI
29.73
Type
journal article
Language
en
Update notices
erratum (2018-05-01)
Open access
Locations
  • Cell Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • Gredos (University of Salamanca) (acceptedVersion, other-oa)
  • Hispana (acceptedVersion, other-oa)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • NIH (2P01AG025532)
  • UC Davis (U2CDK092993)
Topics
  • Health Sciences › Medicine › Physiology › Diet and metabolism studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Health Sciences › Medicine › Physiology › Dietary Effects on Health
Keywords
ketogenic diet, low-carbohydrate diet, longevity, healthspan, lifespan extension, aging, fatty acid beta-oxidation, glycolysis, protein acetylation, mTORC1 signaling, aged mice, physiological function
MeSH terms
Mechanistic Target of Rapamycin Complex 1, Acetylation, Adaptation, Physiological, Animals, Health, Longevity, Male, Mice, Inbred C57BL, Organ Specificity, Signal Transduction, Diet, Carbohydrate-Restricted, Diet, Ketogenic

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