Direct evidence of a role for Nox2 in superoxide production, reduced nitric oxide bioavailability, and early atherosclerotic plaque formation in ApoE−/−mice

Judkins, Courtney P.; Diep, Henry; Broughton, Brad R. S.; Mast, Anja E.; Hooker, Elizabeth U.; Miller, Alyson A.; Selemidis, Stavros; Dusting, Gregory J.; Sobey, Christopher G.; Drummond, Grant R.

doi:10.1152/ajpheart.00799.2009

Cited by 299Nitric Oxide and Endothelin EffectsAtherosclerosis and Cardiovascular DiseasesNeutrophil, Myeloperoxidase and Oxidative Mechanisms

Direct evidence of a role for Nox2 in superoxide production, reduced nitric oxide bioavailability, and early atherosclerotic plaque formation in ApoE−/−mice

Judkins, Courtney P., Diep, Henry, Broughton, Brad R. S., Mast, Anja E., Hooker, Elizabeth U., Miller, Alyson A., Selemidis, Stavros, Dusting, Gregory J. +2 more·2010·American Journal of Physiology-Heart and Circulatory Physiology

DOI 10.1152/ajpheart.00799.2009

Open this paper in CoChat — free account

Abstract

The Nox family NADPH oxidases are reactive oxygen species (ROS)-generating enzymes that are strongly implicated in atherogenesis. However, no studies have examined which Nox isoform(s) are involved. Here we investigated the role of the Nox2-containing NADPH oxidase in atherogenesis in apolipoprotein E-null (ApoE−/−) mice. Wild-type (C57Bl6/J), ApoE−/−, and Nox2−/y/ApoE−/−mice were maintained on a high-fat (21%) diet from 5 wk of age until they were 12 or 19 wk old. Mice were euthanized and their aortas removed for measurement of Nox2 expression (Western blot analysis and immunohistochemistry), ROS production (L012-enhanced chemiluminescence), nitric oxide (NO) bioavailability (contractions to N ω-nitro-l-arginine), and atherosclerotic plaque development along the aorta and in the aortic sinus. Nox2 expression was upregulated in the aortic endothelium of ApoE−/−mice before the appearance of lesions, and this was associated with elevated ROS levels. Within developing plaques, macrophages were also a prominent source of Nox2. The absence of Nox2 in Nox2−/y/ApoE−/−double-knockout mice had minimal effects on plasma lipids or lesion development in the aortic sinus in animals up to 19 wk of age. However, an en face examination of the aorta from the arch to the iliac bifurcation revealed a 50% reduction in lesion area in Nox2−/y/ApoE−/−versus ApoE−/−mice, and this was associated with a marked decrease in aortic ROS production and an increased NO bioavailability. In conclusion, this is the first demonstration of a role for Nox2-NADPH oxidase in vascular ROS production, reduced NO bioavailability, and early lesion development in ApoE−/−mice, highlighting this Nox isoform as a potential target for future therapies for atherosclerosis.

Integrity signals

Registry

Crossref · 10.1152/ajpheart.00799.2009

Cites

30 of 30 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

The combination of NOX2−/− with ApoE−/− can decrease atherosclerotic lesions, but this is not always the case.
“The combination of NOX2−/− with ApoE−/− can decrease the atherosclerotic lesions (475), but this is not always the case (508).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [475]

The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology
Bedard, K., & Krause, K.-H. (2007) · Physiological Reviews · doi:10.1152/physrev.00044.2005 · Cited by 6,989 · see citing works
Endothelial Nitric Oxide Synthase in Vascular Disease: From Marvel to Menace
Förstermann, U., & Münzel, T. (2006) · Circulation · doi:10.1161/circulationaha.105.602532 · Cited by 1,957 · cited 1× · see citing works
“, oxygen reduction is uncoupled from L-arginine oxidation such that it switches from an antiatherogenic NO-producing enzyme to one that generates superoxide, thereby itself becoming a contributor to vascular oxidative stress (13).”
Background
Mouse model of x-linked chronic granulomatous disease, an inherited defect in phagocyte superoxide production
Pollock, J. D., Williams, D. A., Gifford, M. A. C., Li, L. L., Du, X., Fisherman, J., Orkin, S. H., Doerschuk, C. M., & Dinauer, M. C. (1995) · Nature Genetics · doi:10.1038/ng0295-202 · Cited by 921 · see citing works
Superoxide Production and Expression of Nox Family Proteins in Human Atherosclerosis
Sorescu, D., Weiss, D., Lassègue, B., Clempus, R. E., Szöcs, K., Sorescu, G. P., Valppu, L., Quinn, M. T., Lambeth, J. D., Vega, J. D., et al. (2002) · Circulation · doi:10.1161/01.cir.0000012917.74432.66 · Cited by 847 · see citing works
Functional analysis of Nox4 reveals unique characteristics compared to other NADPH oxidases
Martyn, K. D., Frederick, L. M., von Loehneysen, K., Dinauer, M. C., & Knaus, U. G. (2006) · Cellular Signalling · doi:10.1016/j.cellsig.2005.03.023 · Cited by 783 · see citing works
Endothelial Regulation of Vasomotion in ApoE-Deficient Mice: Implications for Interactions Between Peroxynitrite and Tetrahydrobiopterin
Laursen, J. B., Somers, M., Kurz, S., McCann, L., Warnholtz, A., Freeman, B. A., Tarpey, M., Fukai, T., & Harrison, D. G. (2001) · Circulation · doi:10.1161/01.cir.103.9.1282 · Cited by 721 · see citing works
Effects of Angiotensin II Infusion on the Expression and Function of NAD(P)H Oxidase and Components of Nitric Oxide/cGMP Signaling
Mollnau, H., Wendt, M., Szöcs, K., Lassègue, B., Schulz, E., Oelze, M., Li, H., Bodenschatz, M., August, M., Kleschyov, A. L., et al. (2002) · Circulation Research · doi:10.1161/01.res.0000012569.55432.02 · Cited by 676 · see citing works
Accelerated Atherosclerosis, Aortic Aneurysm Formation, and Ischemic Heart Disease in Apolipoprotein E/Endothelial Nitric Oxide Synthase Double-Knockout Mice
Kuhlencordt, P. J., Gyurko, R., Han, F., Scherrer‐Crosbie, M., Aretz, T. H., Hajjar, R. J., Picard, M. H., & Huang, P. L. (2001) · Circulation · doi:10.1161/hc2901.091399 · Cited by 631 · see citing works
Direct Interaction of the Novel Nox Proteins with p22phox Is Required for the Formation of a Functionally Active NADPH Oxidase
Ambasta, R. K., Kumar, P., Griendling, K. K., Schmidt, H. H. H. W., Busse, R., & Brandes, R. P. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m406486200 · Cited by 541 · see citing works
p47phox is required for atherosclerotic lesion progression in ApoE–/– mice
Barry-Lane, P. A., Patterson, C., van der Merwe, M., Hu, Z., Holland, S. M., Yeh, E. T. H., & Runge, M. S. (2001) · Journal of Clinical Investigation · doi:10.1172/jci200111927 · Cited by 489 · see citing works
Redox Control of Endothelial Function and Dysfunction: Molecular Mechanisms and Therapeutic Opportunities
Thomas, S. R., Witting, P. K., & Drummond, G. R. (2008) · Antioxidants & Redox Signaling · doi:10.1089/ars.2008.2027 · Cited by 383 · see citing works
Self-citation
Regulation of Superoxide Anion Production by NADPH Oxidase in Monocytes/Macrophages
Cathcart, M. K. (2003) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000097769.47306.12 · Cited by 365 · see citing works
Redox Mechanisms in Blood Vessels
Mueller, C. F. H., Laude, K. M., McNally, J. S., & Harrison, D. G. (2004) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000149143.04821.eb · Cited by 342 · see citing works
NADPH oxidases in the vasculature: Molecular features, roles in disease and pharmacological inhibition
Selemidis, S., Sobey, C. G., Wingler, K., Schmidt, H. H. H. W., & Drummond, G. R. (2008) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2008.08.005 · Cited by 241 · see citing works
Self-citation
Measurement of NAD(P)H oxidase-derived superoxide with the luminol analogue L-012
Daiber, A., August, M., Baldus, S. E., Wendt, M., Oelze, M. W., Sydow, K., Kleschyov, A. L., & Münzel, T. F. (2003) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2003.10.012 · Cited by 184 · see citing works
Atherosclerosis Is Attenuated by Limiting Superoxide Generation in Both Macrophages and Vessel Wall Cells
Vendrov, A. E., Hakim, Z. S., Madamanchi, N. R., Rojas, M., Madamanchi, C., & Runge, M. S. (2007) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/atvbaha.107.152629 · Cited by 173 · see citing works
Vitamin-mineral supplementation and the progression of atherosclerosis: a meta-analysis of randomized controlled trials
Bleys, J., Miller, E. R., Pastor‐Barriuso, R., Appel, L. J., & Güallar, E. (2006) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/84.4.880 · Cited by 173 · see citing works
Impaired Superoxide Production Due to a Deficiency in Phagocyte NADPH Oxidase Fails to Inhibit Atherosclerosis in Mice
Kirk, E. A., Dinauer, M. C., Rosen, H., Chait, A., Heinecke, J. W., & LeBoeuf, R. C. (2000) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.20.6.1529 · Cited by 171 · see citing works
Vascular Effects Following Homozygous Disruption of p47 <sup>phox</sup>: An Essential Component of NADPH Oxidase
Hsich, E., Segal, B. H., Pagano, P. J., Rey, F. E., Paigen, B., Deleonardis, J., Hoyt, R. F., Holland, S. M., & Finkel, T. (2000) · Circulation · doi:10.1161/01.cir.101.11.1234 · Cited by 158 · see citing works
Novel isoforms of NADPH oxidase in vascular physiology and pathophysiology
BENGTSSON, S. H. M., Gulluyan, L. M., Dusting, G. J., & Drummond, G. R. (2003) · Clinical and Experimental Pharmacology and Physiology · doi:10.1046/j.1440-1681.2003.03929.x · Cited by 135 · see citing works
Self-citation
Differential effects of diabetes on the expression of the gp91phox homologues nox1 and nox4
Wendt, M. C., Daiber, A., Kleschyov, A. L., Mülsch, A., Sydow, K., Schulz, E., Chen, K., Keaney, J. F., Lassègue, B., Walter, U., et al. (2005) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2005.03.020 · Cited by 122 · see citing works
Oxysterol-induced activation of macrophage NADPH-oxidase enhances cell-mediated oxidation of LDL in the atherosclerotic apolipoprotein E deficient mouse: inhibitory role for vitamin E
Rosenblat, M., & Aviram, M. (2002) · Atherosclerosis · doi:10.1016/s0021-9150(01)00563-9 · Cited by 100 · see citing works
Noxa1 is a central component of the smooth muscle NADPH oxidase in mice
Ambasta, R. K., Schreiber, J. G., Janiszewski, M., Busse, R., & Brandes, R. P. (2006) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2005.12.035 · Cited by 90 · see citing works
Gender Influences Cerebral Vascular Responses to Angiotensin II Through Nox2-Derived Reactive Oxygen Species
Silva, T. M. D., Broughton, B. R. S., Drummond, G. R., Sobey, C. G., & Miller, A. A. (2009) · Stroke · doi:10.1161/strokeaha.108.531707 · Cited by 83 · see citing works
Self-citation
Composition and Functions of Vascular Nicotinamide Adenine Dinucleotide Phosphate Oxidases
Brandes, R. P., & Schröder, K. (2008) · Trends in Cardiovascular Medicine · doi:10.1016/j.tcm.2007.11.001 · Cited by 67 · see citing works
Cloning of murine gp91phox cDNA and functional expression in a human X- linked chronic granulomatous disease cell line
Björgvinsdóttir, H., Zhen, L., & Dinauer, M. (1996) · Blood · doi:10.1182/blood.v87.5.2005.2005 · Cited by 63 · see citing works
The ‘A’s and ‘O’s of NADPH oxidase regulation: A commentary on “Subcellular localization and function of alternatively spliced Noxo1 isoforms”
Opitz, N., Drummond, G. R., Selemidis, S., Meurer, S., & Schmidt, H. H. H. W. (2006) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2006.11.003 · Cited by 60 · see citing works
Self-citation
Oxidative Stress and Compartment of Gene Expression Determine Proatherosclerotic Effects of Inducible Nitric Oxide Synthase
Ponnuswamy, P., Ostermeier, E., Schröttle, A., Chen, J., Huang, P. L., Ertl, G., Nieswandt, B., & Kuhlencordt, P. J. (2009) · American Journal Of Pathology · doi:10.2353/ajpath.2009.080730 · Cited by 60 · see citing works
Subcellular localization and function of alternatively spliced Noxo1 isoforms
Ueyama, T., Lekstrom, K. J., Tsujibe, S., Saito, N., & Leto, T. L. (2006) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2006.08.024 · Cited by 59 · see citing works
In vitro knockout of human p47phox blocks superoxide anion production and LDL oxidation by activated human monocytes
Bey, E. A., & Cathcart, M. K. (2000) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34488-6 · Cited by 45 · see citing works

Flagged first, then by citations

Sorted by citations

Reactive Oxygen Species in Metabolic and Inflammatory Signaling
Forrester, S. J., Kikuchi, D. S., Hernandes, M. S., Xu, Q., & Griendling, K. K. (2018) · Circulation Research · doi:10.1161/circresaha.117.311401 · Cited by 2,081 · see citing works
Roles of vascular oxidative stress and nitric oxide in the pathogenesis of atherosclerosis
Förstermann, U., Xia, N., & Li, H. (2017) · Circulation Research · doi:10.1161/circresaha.116.309326 · Cited by 1,519 · see citing works
Role of smooth muscle cells in vascular calcification: implications in atherosclerosis and arterial stiffness
Durham, A. L., Speer, M. Y., Scatena, M., Giachelli, C. M., & Shanahan, C. M. (2018) · Cardiovascular Research · doi:10.1093/cvr/cvy010 · Cited by 1,131 · see citing works
Combating oxidative stress in vascular disease: NADPH oxidases as therapeutic targets
Drummond, G. R., Selemidis, S., Griendling, K. K., & Sobey, C. G. (2011) · Nature Reviews Drug Discovery · doi:10.1038/nrd3403 · Cited by 899 · see citing works
Self-citation
Oxidative Stress in Cardiovascular Diseases: Still a Therapeutic Target?
Senoner, T., & Dichtl, W. (2019) · Nutrients · doi:10.3390/nu11092090 · Cited by 880 · see citing works
Reactive oxygen species in cardiovascular disease
Sugamura, K., & Keaney (2011) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2011.05.004 · Cited by 769 · see citing works
Biochemistry, Physiology, and Pathophysiology of NADPH Oxidases in the Cardiovascular System
Lassegue, B. P., Martín, A. S., & Griendling, K. K. (2012) · Circulation Research · doi:10.1161/circresaha.111.243972 · Cited by 768 · see citing works
Vascular oxidative stress, nitric oxide and atherosclerosis
Li, H., Horke, S., & Förstermann, U. (2014) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2014.09.001 · Cited by 656 · see citing works
NADPH Oxidases: Functions and Pathologies in the Vasculature
Lassègue, B., & Griendling, K. K. (2010) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/atvbaha.108.181610 · Cited by 592 · see citing works
Endothelial Cell Metabolism
Eelen, G., de Zeeuw, P., Treps, L., Harjes, U., Wong, B. W., & Carmeliet, P. (2017) · Physiological Reviews · doi:10.1152/physrev.00001.2017 · Cited by 591 · see citing works
The Role of Oxidative Stress in Atherosclerosis
Batty, M., Bennett, M. R., & Yu, E. (2022) · Cells · doi:10.3390/cells11233843 · Cited by 569 · see citing works
Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation
Tejero, J., Shiva, S., & Gladwin, M. T. (2019) · Physiological Reviews · doi:10.1152/physrev.00036.2017 · Cited by 541 · see citing works
NADPH oxidases and oxidase crosstalk in cardiovascular diseases: novel therapeutic targets
Zhang, Y., Murugesan, P., Huang, K., & Cai, H. “. (2019) · Nature Reviews Cardiology · doi:10.1038/s41569-019-0260-8 · Cited by 546 · see citing works
Evolution of nadph oxidase inhibitors: Selectivity and mechanisms for target engagement
Altenhöfer, S., Radermacher, K. A., Kleikers, P. W. M., Wingler, K., & Schmidt, H. H. H. W. (2015) · Antioxidants & Redox Signaling · doi:10.1089/ars.2013.5814 · Cited by 521 · see citing works
Molecular Biology of Atherosclerosis
Hopkins, P. N. (2013) · Physiological Reviews · doi:10.1152/physrev.00004.2012 · Cited by 456 · see citing works
NLRP3 inflammasome: Its regulation and involvement in atherosclerosis
Hoseini, Z. S., Sepahvand, F., Rashidi, B., Sahebkar, A., Masoudifar, A., & Mirzaei, H. (2017) · Journal of Cellular Physiology · doi:10.1002/jcp.25930 · Cited by 458 · see citing works
NADPH Oxidases in Vascular Pathology
Konior, A., Schramm‐Luc, A., Cześnikiewicz‐Guzik, M., & Guzik, T. J. (2013) · Antioxidants and Redox Signaling · doi:10.1089/ars.2013.5607 · Cited by 451 · see citing works
Oxidative Stress-Induced Endothelial Dysfunction in Cardiovascular Diseases
Shaito, A. A., Aramouni, K., Assaf, R., Parenti, A., Orekhov, A. N., EL‐Yazbi, A. F., Pintus, G., & Eid, A. H. (2022) · Frontiers in Bioscience-Landmark · doi:10.31083/j.fbl2703105 · Cited by 427 · see citing works
NADPH Oxidase 1 Plays a Key Role in Diabetes Mellitus–Accelerated Atherosclerosis
Gray, S. P., Di Marco, E., Okabe, J., Szyndralewiez, C., Heitz, F., Montezano, A. C., de Haan, J. B., Koulis, C., El-Osta, A., Andrews, K. L., et al. (2013) · Circulation · doi:10.1161/circulationaha.112.132159 · Cited by 386 · see citing works
Anti-inflammatory therapies for atherosclerosis
Bäck, M., & Hansson, G. K. (2015) · Nature Reviews Cardiology · doi:10.1038/nrcardio.2015.5 · Cited by 380 · see citing works
Redox control of inflammation in macrophages
Brüne, B., Dehne, N., Grossmann, N., Jung, M., Namgaladze, D., Schmid, T., von Knethen, A., & Weigert, A. (2013) · Antioxidants & Redox Signaling · doi:10.1089/ars.2012.4785 · Cited by 377 · see citing works
The Renin-Angiotensin-Aldosterone System in Vascular Inflammation and Remodeling
Pacurari, M., Kafoury, R., Tchounwou, P. B., & Ndebele, K. (2014) · International Journal of Inflammation · doi:10.1155/2014/689360 · Cited by 377 · see citing works
Endothelial NADPH oxidases: which NOX to target in vascular disease?
Drummond, G. R., & Sobey, C. G. (2014) · Trends in Endocrinology & Metabolism · doi:10.1016/j.tem.2014.06.012 · Cited by 315 · see citing works
Self-citation
CVD and Oxidative Stress
Cervantes-Gracia, K., Llanas-Cornejo, D., & Husi, H. (2017) · Journal of Clinical Medicine · doi:10.3390/jcm6020022 · Cited by 314 · see citing works
NADPH oxidase links endoplasmic reticulum stress, oxidative stress, and PKR activation to induce apoptosis
Li, G., Scull, C. M., Özcan, L., & Tabas, I. A. (2010) · The Journal of Cell Biology · doi:10.1083/jcb.201006121 · Cited by 306 · see citing works
Protective Effects of Estrogen on Cardiovascular Disease Mediated by Oxidative Stress
Xiang, D., Liu, Y., Zhou, S.-J., Zhou, E., & Wang, Y. (2021) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2021/5523516 · Cited by 262 · see citing works
Macrophage Mitochondrial Oxidative Stress Promotes Atherosclerosis and Nuclear Factor-κB–Mediated Inflammation in Macrophages
Wang, Y., Wang, G. Z., Rabinovitch, P. S., & Tabas, I. A. (2013) · Circulation Research · doi:10.1161/circresaha.114.302153 · Cited by 264 · see citing works
NADPH oxidases in cardiovascular disease
Brandes, R. P., Weißmann, N., & Schröder, K. (2010) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2010.04.030 · Cited by 266 · see citing works
Pro-inflammatory hepatic macrophages generate ROS through NADPH oxidase 2 via endocytosis of monomeric TLR4–MD2 complex
Kim, S. Y., Jeong, J., Kim, S. J., Seo, W., Kim, M., Choi, W., Yoo, W., Lee, J. H., Shim, Y., Yi, H., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-02325-2 · Cited by 258 · see citing works
Vascular Oxidative Stress: Impact and Therapeutic Approaches
Sena, C. M., Leandro, A., Azul, L., Seiça, R. M. F., & Perry, G. L. (2018) · Frontiers in Physiology · doi:10.3389/fphys.2018.01668 · Cited by 260 · see citing works
Redox Roles of Reactive Oxygen Species in Cardiovascular Diseases
He, F. J., & Zuo, L. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms161126059 · Cited by 240 · see citing works
Redox signaling in cardiovascular health and disease
Madamanchi, N. R., & Runge, M. S. (2013) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2013.04.001 · Cited by 223 · see citing works
NOX4 NADPH Oxidase-Dependent Mitochondrial Oxidative Stress in Aging-Associated Cardiovascular Disease
Vendrov, A. E., Vendrov, K. C., Smith, A., Yuan, J., Sumida, A., Robidoux, J., Runge, M. S., & Madamanchi, N. R. (2015) · Antioxidants and Redox Signaling · doi:10.1089/ars.2014.6221 · Cited by 219 · see citing works
Oxidative stress and cardiovascular disease: new insights
Pignatelli, P., Menichelli, D., Pastori, D., & Violi, F. (2018) · Kardiologia Polska · doi:10.5603/kp.a2018.0071 · Cited by 212 · see citing works
Discovery of GSK2795039, a Novel Small Molecule NADPH Oxidase 2 Inhibitor
Hirano, K., Chen, W. S., Chueng, A. L. W., Dunne, A. A., Seredenina, T., Filippova, A., Ramachandran, S., Bridges, A., Chaudry, L., Pettman, G., et al. (2015) · Antioxidants & Redox Signaling · doi:10.1089/ars.2014.6202 · Cited by 205 · see citing works
Reactive Oxygen Species Can Provide Atheroprotection via NOX4-Dependent Inhibition of Inflammation and Vascular Remodeling
Gray, S. P., Di Marco, E., Kennedy, K., Chew, P., Okabe, J., El-Osta, A., Calkin, A. C., Biessen, E. A. L., Touyz, R. M., Cooper, M. E., et al. (2016) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/atvbaha.115.307012 · Cited by 189 · see citing works
NADPH Oxidases: From Molecular Mechanisms to Current Inhibitors
Cipriano, A., Viviano, M., Feoli, A., Milite, C., Sarno, G., Castellano, S., & Sbardella, G. (2023) · Journal of Medicinal Chemistry · doi:10.1021/acs.jmedchem.3c00770 · Cited by 190 · see citing works
NADPH oxidases in cardiovascular disease: insights from in vivo models and clinical studies
Sirker, A. A., Zhang, M., & Shah, A. M. (2011) · Basic Research in Cardiology · doi:10.1007/s00395-011-0190-z · Cited by 180 · see citing works
The NADPH oxidase Nox4 has anti-atherosclerotic functions
Schürmann, C., Rezende, F., Kruse, C., Yasar, Y., Löwe, O., Fork, C., van de Sluis, B., Bremer, R., Weißmann, N., Shah, A. M., et al. (2015) · European Heart Journal · doi:10.1093/eurheartj/ehv460 · Cited by 178 · see citing works
From form to function: the role of Nox4 in the cardiovascular system
Chen, F., Haigh, S. B., Barman, S. A., & Fulton, D. J. (2012) · Frontiers in Physiology · doi:10.3389/fphys.2012.00412 · Cited by 172 · see citing works
Rac1 in human diseases: The therapeutic potential of targeting Rac1 signaling regulatory mechanisms
Marei, H., & Malliri, A. (2016) · Small GTPases · doi:10.1080/21541248.2016.1211398 · Cited by 168 · see citing works
Redox signaling in cardiovascular pathophysiology: A focus on hydrogen peroxide and vascular smooth muscle cells
Byon, C. H., Heath, J. M., & Chen, Y. (2016) · Redox Biology · doi:10.1016/j.redox.2016.08.015 · Cited by 164 · see citing works
Recent novel approaches to limit oxidative stress and inflammation in diabetic complications
Pickering, R. J., Rosado, C. J., Sharma, A., Buksh, S., Tate, M., & de Haan, J. B. (2018) · Clinical & Translational Immunology · doi:10.1002/cti2.1016 · Cited by 164 · see citing works
Dysfunction of Cerebrovascular Endothelial Cells: Prelude to Vascular Dementia
Wang, F., Cao, Y., Ma, L., Pei, H., Rausch, W., & Li, H. (2018) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2018.00376 · Cited by 160 · see citing works
Scavenger receptor CD36 is essential for the cerebrovascular oxidative stress and neurovascular dysfunction induced by amyloid-β
Park, L., Wang, G., Zhou, P., Zhou, J., Pitstick, R., Previti, M. L., Younkin, L., Younkin, S. G., Van Nostrand, W. E., Cho, S., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1015413108 · Cited by 156 · see citing works
Endosomal nox2 oxidase exacerbates virus pathogenicity and is a target for antiviral therapy
To, E. E., Vlahos, R., Luong, R., Halls, M. L., Reading, P. C., King, P. T., Chan, C., Drummond, G. R., Sobey, C. G., Broughton, B. R. S., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-00057-x · Cited by 154 · see citing works
Self-citation
Lipid Peroxidation in Atherosclerotic Cardiovascular Diseases
Gianazza, E., Brioschi, M., Fernandez, A. M., Casalnuovo, F., Altomare, A., Aldini, G., & Banfi, C. (2020) · Antioxidants and Redox Signaling · doi:10.1089/ars.2019.7955 · Cited by 158 · see citing works
ATP-Binding Membrane Cassette Transporter A1 (ABCA1): A Possible Link between Inflammation and Reverse Cholesterol Transport
Yin, K., Liao, D., & Tang, C. (2010) · Molecular Medicine · doi:10.2119/molmed.2010.00004 · Cited by 152 · see citing works
Role for Nox1 NADPH oxidase in atherosclerosis
Sheehan, A. L., Carrell, S., Johnson, B., Stanić, B., Bánfi, B., & Miller, F. J. (2011) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2011.02.028 · Cited by 148 · see citing works
The Role of Oxidative Stress and Autophagy in Atherosclerosis
Perrotta, I. D., & Aquila, S. (2015) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2015/130315 · Cited by 147 · see citing works
NADPH oxidases: key modulators in aging and age-related cardiovascular diseases?
Sahoo, S., Meijles, D. N., & Pagano, P. J. (2016) · Clinical Science · doi:10.1042/cs20150087 · Cited by 146 · see citing works
The Nox Family of NADPH Oxidases: Friend or Foe of the Vascular System?
Takac, I., Schröder, K., & Brandes, R. P. (2011) · Current Hypertension Reports · doi:10.1007/s11906-011-0238-3 · Cited by 142 · see citing works
Role of Vascular Oxidative Stress in Obesity and Metabolic Syndrome
Youn, J.-Y., Siu, K. L., Lob, H. E., Itani, H. A., Harrison, D. G., & Cai, H. “. (2014) · Diabetes · doi:10.2337/db13-0719 · Cited by 138 · see citing works
NADPH oxidases and vascular remodeling in cardiovascular diseases
García‐Redondo, A. B., Aguado, A., Briones, A. M., & Salaíces, M. (2016) · Pharmacological Research · doi:10.1016/j.phrs.2016.10.015 · Cited by 137 · see citing works
NADPH oxidase 4 protects against development of endothelial dysfunction and atherosclerosis in LDL receptor deficient mice
Langbein, H., Brunssen, C., Hofmann, A., Cimalla, P., Brux, M., Bornstein, S. R., Deussen, A., Koch, E., & Morawietz, H. (2016) · European Heart Journal · doi:10.1093/eurheartj/ehv564 · Cited by 133 · see citing works
Endothelial Cell–Specific Reactive Oxygen Species Production Increases Susceptibility to Aortic Dissection
Fan, L. M., Douglas, G., Bendall, J. K., McNeill, E., Crabtree, M. J., Hale, A., Mai, A., Li, J., McAteer, M. A., Schneider, J. E., et al. (2014) · Circulation · doi:10.1161/circulationaha.113.005062 · Cited by 134 · see citing works
Oxidative Stress in Cardiovascular Diseases and Obesity: Role of p66Shc and Protein Kinase C
Marchi, E. D., Baldassari, F., Bononi, A., Wieckowski, M. R., & Pinton, P. (2013) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2013/564961 · Cited by 132 · see citing works
Oxidative Stress in Cell Death and Cardiovascular Diseases
Xu, T., Ding, W., Ji, X., Ao, X., Liu, Y., Yu, W., & Wang, J. (2019) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2019/9030563 · Cited by 131 · see citing works
The NADPH Oxidase Family and its Inhibitors
Kleniewska, P., Piechota-Polańczyk, A., Skibska, B., & Gorąca, A. (2012) · Archivum Immunologiae et Therapiae Experimentalis · doi:10.1007/s00005-012-0176-z · Cited by 126 · see citing works
Oxidative Stress in Cardiovascular Disease
Csányi, G., & Jr., F. (2014) · International Journal of Molecular Sciences · doi:10.3390/ijms15046002 · Cited by 130 · see citing works
On the use of L-012, a luminol-based chemiluminescent probe, for detecting superoxide and identifying inhibitors of NADPH oxidase: a reevaluation
Zielonka, J., Lambeth, J. D., & Kalyanaraman, B. (2013) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2013.09.017 · Cited by 128 · see citing works
Redox regulation of the actin cytoskeleton and its role in the vascular system
Xu, Q., Huff, L. P., Fujii, M., & Griendling, K. K. (2017) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2017.03.004 · Cited by 129 · see citing works
Actions of “antioxidants” in the protection against atherosclerosis
Lönn, M. E., Dennis, J. M., & Stocker, R. (2012) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2012.05.027 · Cited by 124 · see citing works
Nitric oxide and mitochondria in metabolic syndrome
Litvinova, L. S., Atochin, D. N., Fattakhov, N. S., Василенко, М., Затолокин, П. А., & Кириенкова, Е. В. (2015) · Frontiers in Physiology · doi:10.3389/fphys.2015.00020 · Cited by 127 · see citing works
Impairment of both nitric oxide‐mediated and EDHF‐type relaxation in small mesenteric arteries from rats with streptozotocin‐induced diabetes
Leo, C., Hart, J. L., & Woodman, O. L. (2010) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2010.01023.x · Cited by 122 · see citing works
Evolving Concepts of Oxidative Stress and Reactive Oxygen Species in Cardiovascular Disease
Chen, K., & Keaney, J. F. (2012) · Current Atherosclerosis Reports · doi:10.1007/s11883-012-0266-8 · Cited by 119 · see citing works
Atherothrombosis and Oxidative Stress: Mechanisms and Management in Elderly
Violi, F., Loffredo, L., Carnevale, R., Pignatelli, P., & Pastori, D. (2017) · Antioxidants and Redox Signaling · doi:10.1089/ars.2016.6963 · Cited by 121 · see citing works
Oxidative Stress, Nox Isoforms and Complications of Diabetes—Potential Targets for Novel Therapies
Sedeek, M. H., Montezano, A. C. I., Hébert, R., Gray, S. P., Marco, E. D., Jha, J. C., Cooper, M. E., Jandeleit-Dahm, K. A. M., Schiffrin, E. L., Wilkinson‐Berka, J. L., et al. (2012) · Journal of Cardiovascular Translational Research · doi:10.1007/s12265-012-9387-2 · Cited by 116 · see citing works
Regulation of NAD(P)H oxidases by AMPK in cardiovascular systems
Song, P., & Zou, M. (2012) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2012.01.025 · Cited by 119 · see citing works
Quercetin attenuates high fat diet-induced atherosclerosis in apolipoprotein E knockout mice: A critical role of NADPH oxidase
Xiao, L., Liu, L., Guo, X., Zhang, S., Wang, J., Zhou, F., Liu, L., Tang, Y., & Yao, P. (2017) · Food and Chemical Toxicology · doi:10.1016/j.fct.2017.03.048 · Cited by 113 · see citing works
Endothelial-specific Nox2 overexpression increases vascular superoxide and macrophage recruitment in ApoE−/− mice
Douglas, G., Bendall, J. K., Crabtree, M. J., Tatham, A. L., Carter, E. E., Hale, A. B., & Channon, K. M. (2012) · Cardiovascular Research · doi:10.1093/cvr/cvs026 · Cited by 111 · see citing works
Role of oxidative stress in calcific aortic valve disease and its therapeutic implications
Greenberg, H. Z. E., Zhao, G., Shah, A. M., & Zhang, M. (2021) · Cardiovascular Research · doi:10.1093/cvr/cvab142 · Cited by 110 · see citing works
S100A12 in Vascular Smooth Muscle Accelerates Vascular Calcification in Apolipoprotein E–Null Mice by Activating an Osteogenic Gene Regulatory Program
Hofmann Bowman, M. A., Gawdzik, J., Bukhari, U., Husain, A. N., Toth, P. T., Kim, G., Earley, J., & McNally, E. M. (2011) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/atvbaha.110.217745 · Cited by 111 · see citing works
Upregulation of phase II enzymes through phytochemical activation of Nrf2 protects cardiomyocytes against oxidant stress
Reuland, D. J., Khademi, S., Castle, C. J., Irwin, D. C., McCord, J. M., Miller, B. F., & Hamilton, K. L. (2012) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2012.11.016 · Cited by 110 · see citing works
Emerging role of PKA/eNOS pathway in therapeutic angiogenesis for ischaemic tissue diseases
Bir, S. C., Xiong, Y., Kevil, C. G., & Luo, J. (2012) · Cardiovascular Research · doi:10.1093/cvr/cvs143 · Cited by 107 · see citing works
ROS and RNS Signaling in Heart Disorders: Could Antioxidant Treatment Be Successful?
Afanasʹev, I. B. (2011) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2011/293769 · Cited by 106 · see citing works
Nox isoforms in vascular pathophysiology: insights from transgenic and knockout mouse models
Rivera, J., Sobey, C. G., Walduck, A. K., & Drummond, G. R. (2010) · Redox Report · doi:10.1179/174329210x12650506623401 · Cited by 105 · see citing works
Self-citation
IL-4 and IL-13 induce equivalent expression of traditional M2 markers and modulation of reactive oxygen species in human macrophages
Scott, T., Lewis, C., Zhu, M., Wang, C., Samuel, C. S., Drummond, G. R., & Kemp‐Harper, B. K. (2023) · Scientific Reports · doi:10.1038/s41598-023-46237-2 · Cited by 106 · see citing works
Self-citation
NADPH oxidases: redox regulation of cell homeostasis and disease
Kračun, D., Lopes, L. R., Cifuentes-Pagano, E., & Pagano, P. J. (2025) · Physiological Reviews · doi:10.1152/physrev.00034.2023 · Cited by 104 · see citing works
CB1 receptor inhibition leads to decreased vascular AT1 receptor expression, inhibition of oxidative stress and improved endothelial function
Tiyerili, V., Zimmer, S., Jung, S., Wassmann, K., Naehle, C. P., Lütjohann, D., Zimmer, A., Nickenig, G., & Waßmann, S. (2010) · Basic Research in Cardiology · doi:10.1007/s00395-010-0090-7 · Cited by 99 · see citing works
Dihydromyricetin ameliorates atherosclerosis in LDL receptor deficient mice
Liu, T. T., Zeng, Y., Tang, K., Chen, X., Zhang, W., & Xu, X. L. (2017) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2017.05.003 · Cited by 100 · see citing works
NADPH Oxidase-2 and Atherothrombosis
Violi, F., Carnevale, R., Loffredo, L., Pignatelli, P., & Gallin, J. I. (2016) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/atvbaha.116.308351 · Cited by 99 · see citing works
NADPH Oxidases as Regulators of Tumor Angiogenesis: Current and Emerging Concepts
Coso, S., Harrison, I., Harrison, C. B., Vinh, A., Sobey, C. G., Drummond, G. R., Williams, E. D., & Selemidis, S. (2012) · Antioxidants and Redox Signaling · doi:10.1089/ars.2011.4489 · Cited by 99 · see citing works
Self-citation
Role of reactive oxygen species in atherosclerosis: Lessons from murine genetic models
Nègre‐Salvayre, A., Guerby, P., Gayral, S., Laffargue, M., & Salvayre, R. (2019) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2019.10.011 · Cited by 98 · see citing works
The opposing roles of NO and oxidative stress in cardiovascular disease
Ritchie, R. H., Drummond, G. R., Sobey, C. G., Silva, T. M. D., & Kemp‐Harper, B. K. (2016) · Pharmacological Research · doi:10.1016/j.phrs.2016.12.017 · Cited by 98 · see citing works
Self-citation
NOX2 As a Target for Drug Development: Indications, Possible Complications, and Progress
Diebold, B. A., Smith, S. M., Yang, L., & Lambeth, J. D. (2014) · Antioxidants and Redox Signaling · doi:10.1089/ars.2014.5862 · Cited by 96 · see citing works
Reactive Oxygen Species in Endothelial Function – From Disease to Adaptation –
Craige, S. M., Kant, S., & Keaney, J. F. (2015) · Circulation Journal · doi:10.1253/circj.cj-15-0464 · Cited by 91 · see citing works
Age-related diseases as vicious cycles
Belikov, A. V. (2018) · Ageing Research Reviews · doi:10.1016/j.arr.2018.11.002 · Cited by 92 · see citing works
Identification of a Non-Growth Factor Role for GM-CSF in Advanced Atherosclerosis
Subramanian, M., Thorp, E. B., & Tabas, I. A. (2014) · Circulation Research · doi:10.1161/circresaha.116.304794 · Cited by 90 · see citing works
Activin and NADPH-oxidase in preeclampsia: insights from in vitro and murine studies
Lim, R., Acharya, R. Y., Delpachitra, P., Hobson, S. R., Sobey, C. G., Drummond, G. R., & Wallace, E. M. (2014) · American Journal of Obstetrics and Gynecology · doi:10.1016/j.ajog.2014.07.021 · Cited by 87 · see citing works
Self-citation
Cannabidiol modulation of oxidative stress and signalling
Pereira, S. R., Hackett, B., O’Driscoll, D. N., Sun, M. C., & Downer, E. J. (2021) · Neuronal Signaling · doi:10.1042/ns20200080 · Cited by 90 · see citing works
The pathobiology of diabetic vascular complications—cardiovascular and kidney disease
Gray, S. P., & Jandeleit-Dahm, K. A. M. (2014) · Journal of Molecular Medicine · doi:10.1007/s00109-014-1146-1 · Cited by 83 · see citing works
Nox2 Oxidase Activity Accounts for the Oxidative Stress and Vasomotor Dysfunction in Mouse Cerebral Arteries following Ischemic Stroke
Silva, T. M. D., Brait, V. H., Drummond, G. R., Sobey, C. G., & Miller, A. A. (2011) · PLoS ONE · doi:10.1371/journal.pone.0028393 · Cited by 78 · see citing works
Self-citation
Myeloperoxidase: A versatile mediator of endothelial dysfunction and therapeutic target during cardiovascular disease
Maiocchi, S. L., Ku, J., Thai, T., Chan, E., Rees, M. D., & Thomas, S. R. (2020) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2020.107711 · Cited by 75 · see citing works
Nox1 in cardiovascular diseases: regulation and pathophysiology
Gimenez, M., Schickling, B., Lopes, L. R., & Miller, F. J. (2015) · Clinical Science · doi:10.1042/cs20150404 · Cited by 75 · see citing works
Nitroxyl (HNO) as a Vasoprotective Signaling Molecule
Bullen, M. L., Miller, A. A., Andrews, K. L., Irvine, J. C., Ritchie, R. H., Sobey, C. G., & Kemp‐Harper, B. K. (2010) · Antioxidants and Redox Signaling · doi:10.1089/ars.2010.3327 · Cited by 74 · see citing works
Self-citation
Augmented Superoxide Production By Nox2-Containing NADPH Oxidase Causes Cerebral Artery Dysfunction During Hypercholesterolemia
Miller, A. A., Silva, T. M. D., Judkins, C. P., Diep, H., Drummond, G. R., & Sobey, C. G. (2010) · Stroke · doi:10.1161/strokeaha.109.575365 · Cited by 74 · see citing works
Self-citation
Shared mechanisms of multimorbidity in COPD, atherosclerosis and type-2 diabetes: the neutrophil as a potential inflammatory target
Hughes, M., McGettrick, H. M., & Sapey, E. (2020) · European Respiratory Review · doi:10.1183/16000617.0102-2019 · Cited by 73 · see citing works
Opportunity Nox: The Future of NADPH Oxidases as Therapeutic Targets in Cardiovascular Disease
Streeter, J., Thiel, W. H., Brieger, K., & Miller, F. J. (2012) · Cardiovascular Therapeutics · doi:10.1111/j.1755-5922.2011.00310.x · Cited by 73 · see citing works
Hemodynamic Regulation of Reactive Oxygen Species: Implications for Vascular Diseases
Raaz, U., Toh, R., Mäegdefessel, L., Adam, M., Nakagami, F., Emrich, F. C., Spin, J. M., & Tsao, P. S. (2013) · Antioxidants and Redox Signaling · doi:10.1089/ars.2013.5507 · Cited by 73 · see citing works

Abstract 5462: Dual Effects of Nox2-NADPH Oxidase During Atherosclerotic Plaque Development in Apolipoprotein E-Null Mice
Judkins, C. P., Diep, H., Broughton, B. R. S., Mast, A., Hooker, E. U., Miller, A. A., Selemidis, S., Dusting, G. J., Sobey, C. G., & Drummond, G. R. (2009) · Circulation · doi:10.1161/circ.120.suppl_18.s1096-c
Self-citation
Nox2‐dependent NADPH oxidase activity contributes to atherogenesis in Apolipoprotein E‐Null (ApoE−/−) mice
Drummond, G. R., Diep, H., Mast, A., Selemidis, S., Dusting, G. J., Sobey, C. G., & Judkins, C. P. (2008) · The FASEB Journal · doi:10.1096/fasebj.22.2_supplement.35
Self-citation
Role for Nox1 NADPH oxidase in atherosclerosis
Sheehan, A. L., Carrell, S., Johnson, B., Stanić, B., Bánfi, B., & Miller, F. J. (2011) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2011.02.028 · Cited by 148 · see citing works
Endothelial-specific Nox2 overexpression increases vascular superoxide and macrophage recruitment in ApoE−/− mice
Douglas, G., Bendall, J. K., Crabtree, M. J., Tatham, A. L., Carter, E. E., Hale, A. B., & Channon, K. M. (2012) · Cardiovascular Research · doi:10.1093/cvr/cvs026 · Cited by 111 · see citing works
Endothelial-specific Nox2 over-expression increases vascular superoxide production but does not alter atherosclerotic plaque or AngII-dependent aneurysm formation
Douglas, G., Bendall, J. K., & Channon, K. M. (2010) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2010.08.025 · Cited by 2 · see citing works

Authors: Judkins, C. P., Diep, H., Broughton, B. R. S., Mast, A. E., Hooker, E. U., Miller, A. A., Selemidis, S., Dusting, G. J., Sobey, C. G., & Drummond, G. R.
Affiliations: Monash University (AU)
The University of Melbourne (AU)
ORCID: Judkins, Courtney P.
Diep, Henry
Broughton, Brad R. S.
Miller, Alyson A.
Selemidis, Stavros
Dusting, Gregory J.
Sobey, Christopher G.
Drummond, Grant R.
Venue: American Journal of Physiology-Heart and Circulatory Physiology
Publisher: American Physiological Society
Published: 2010-01
Volume: 298
Issue: 1
Pages: H24-H32
ISSN: 0363-6135 (ISSN-L)
1522-1539
DOI: 10.1152/ajpheart.00799.2009
OpenAlex: W2157615046
Cited by: 299
References: 30
Citations by year: 2026: 10 · 2025: 11 · 2024: 12 · 2023: 15 · 2022: 10 · 2021: 16 · 2020: 12 · 2019: 11 · 2018: 15 · 2017: 20 · 2016: 23 · 2015: 30 · 2014: 34 · 2013: 23 · 2012: 25
FWCI: 12.37
Type: journal article
Language: en
Open access: closed
Locations: American Journal of Physiology-Heart and Circulatory Physiology (publishedVersion)
PubMed (publishedVersion)
Research Bank (Australian Catholic University) (submittedVersion)
Topics: Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Keywords: NOX2, NADPH oxidase, superoxide production, reactive oxygen species, nitric oxide bioavailability, atherosclerosis, atherosclerotic plaque formation, apolipoprotein E deficiency, macrophages, aortic endothelium, high-fat diet
MeSH terms: NADPH Oxidase 2, Animals, Aorta, Thoracic, Apolipoproteins E, Biological Availability, Enzyme Inhibitors, Hypercholesterolemia, Immunohistochemistry, Isoenzymes, Lipids, Male, Membrane Glycoproteins, Muscle Contraction, Muscle, Smooth, Vascular, Nitric Oxide, Superoxides, Blotting, Western, Reactive Oxygen Species, Mice, Knockout, NADPH Oxidases, NG-Nitroarginine Methyl Ester, Atherosclerosis, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Direct evidence of a role for Nox2 in superoxide production, reduced nitric oxide bioavailability, and early atherosclerotic plaque formation in ApoE−/−mice

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Cites

Cited by

Related

Record

About these checks