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Cited by 39Biochemical effects in animalsSports Performance and TrainingMuscle metabolism and nutrition

Effect of β-alanine supplementation during high-intensity interval training on repeated sprint ability performance and neuromuscular fatigue

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Milioni, Fabio, de Poli, Rodrigo Araújo Bonetti, Saunders, Bryan, Gualano, Bruno, da Rocha, Alisson L., Sanchez Ramos da Silva, Adelino, Muller, Paulo de Tarso Guerrero, Zagatto, Alessandro Moura·2019·Journal of Applied Physiology
DOI 10.1152/japplphysiol.00321.2019
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Abstract

The study investigated the influence of β-alanine supplementation during a high-intensity interval training (HIIT) program on repeated sprint ability (RSA) performance. This study was randomized, double-blinded, and placebo controlled. Eighteen men performed an incremental running test until exhaustion (T INC ) at baseline and followed by 4-wk HIIT (10 × 1-min runs 90% maximal T INC velocity [1-min recovery]). Then, participants were randomized into two groups and performed a 6-wk HIIT associated with supplementation of 6.4 g/day of β-alanine (Gβ) or dextrose (placebo group; GP). Pre- and post-6-wk HIIT + supplementation, participants performed the following tests: 1) T INC ; 2) supramaximal running test; and 3) 2 × 6 × 35-m sprints (RSA). Before and immediately after RSA, neuromuscular function was assessed by vertical jumps, maximal isometric voluntary contractions of knee extension, and neuromuscular electrical stimulations. Muscle biopsies were performed to determine muscle carnosine content, muscle buffering capacity in vitro (βm in vitro ), and content of phosphofructokinase (PFK), monocarboxylate transporter 4 (MCT4), and hypoxia-inducible factor-1α (HIF-1α). Both groups showed a significant time effect for maximal oxygen uptake (Gβ: 6.2 ± 3.6% and GP: 6.5 ± 4.2%; P > 0.01); only Gβ showed a time effect for total (−3.0 ± 2.0%; P = 0.001) and best (−3.3 ± 3.0%; P = 0.03) RSA times. A group-by-time interaction was shown after HIIT + Supplementation for muscle carnosine (Gβ: 34.4 ± 2.3 mmol·kg −1 ·dm −1 and GP: 20.7 ± 3.0 mmol·kg −1 ·dm −1 ; P = 0.003) and neuromuscular voluntary activation after RSA (Gβ: 87.2 ± 3.3% and GP: 78.9 ± 12.4%; P = 0.02). No time effect or group-by-time interaction was shown for supramaximal running test performance, βm, and content of PFK, MCT4, and HIF-1α. In summary, β-alanine supplementation during HIIT increased muscle carnosine and attenuated neuromuscular fatigue, which may contribute to an enhancement of RSA performance. NEW & NOTEWORTHY β-Alanine supplementation during a high-intensity interval training program increased repeated sprint performance. The improvement of muscle carnosine content induced by β-alanine supplementation may have contributed to an attenuation of central fatigue during repeated sprint. Overall, β-alanine supplementation may be a useful dietary intervention to prevent fatigue.

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  • The interplay between neuromuscular fatigue, muscle glycogen depletion, and Pi accumulation further complicates the scenario of repeated sprint performance.

    “The interplay between neuromuscular fatigue, muscle glycogen depletion, and Pi accumulation further complicates this scenario19,21.”

    From Evaluating bioenergetic pathway contributions from single to multiple sprints (2024) · cites this paper as [21]

  • More recent evidence highlights other significant factors, such as phosphate accumulation, muscle glycogen availability, and neuromuscular impairments, all of which contribute to performance decline.

    “While cellular acidosis has traditionally been seen as a major contributor to fatigue, more recent evidence highlights other significant factors, such as phosphate accumulation, muscle glycogen availability, and neuromuscular impairments, all of which contribute to performance decline19,21,37.”

    From Evaluating bioenergetic pathway contributions from single to multiple sprints (2024) · cites this paper as [21]

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Authors
Milioni, F., de Poli, R. A. B., Saunders, B., Gualano, B., da Rocha, A. L., Sanchez Ramos da Silva, A., Muller, P. D. T. G., & Zagatto, A. M.
Affiliations
  • Post Graduate Program in Human Movement Sciences, Laboratory of Physiology and Human Performance, São Paulo State University, Bauru, São Paulo, Brazil
  • Universidade de São Paulo (BR)
  • School of Physical Education and Sports of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
  • Laboratory of Respiratory Pathophysiology, Mato Grosso do Sul Federal University, Campo Grande, Mato Grosso do Sul, São Paulo, Brazil
  • Universidade Estadual Paulista (Unesp) (BR)
Corresponding author
Zagatto, Alessandro Moura
ORCID
Venue
Journal of Applied Physiology
Publisher
American Physiological Society
Published
2019-12-01
Volume
127
Issue
6
Pages
1599-1610
ISSN
  • 0161-7567 (ISSN-L)
  • 8750-7587
  • 1522-1601
DOI
10.1152/japplphysiol.00321.2019
OpenAlex
W2980353017
Cited by
39
References
50
Citations by year
2026: 9 · 2025: 7 · 2024: 6 · 2023: 6 · 2022: 6 · 2021: 4 · 2020: 1
FWCI
1.89
Type
journal article
Language
en
Open access
Licence
other-oa
Locations
  • Journal of Applied Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, other-oa)
Topics
  • Health Sciences › Medicine › Physiology › Biochemical effects in animals
  • Health Sciences › Medicine › Orthopedics and Sports Medicine › Sports Performance and Training
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
Keywords
beta-alanine supplementation, high-intensity interval training, repeated sprint ability, neuromuscular fatigue, muscle carnosine, muscle buffering capacity, voluntary activation, phosphofructokinase, MCT4, hypoxia-inducible factor-1α, VO2max, dextrose placebo, endurance running performance
MeSH terms
High-Intensity Interval Training, Adult, Carnosine, Double-Blind Method, Exercise Test, Humans, Isometric Contraction, Male, Oxygen Consumption, Running, beta-Alanine, Exercise, Muscle, Skeletal, Muscle Fatigue, Dietary Supplements

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