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Cited by 123Muscle Physiology and DisordersEstrogen and related hormone effectsNutrition and Health in Aging

Removal of ovarian hormones from mature mice detrimentally affects muscle contractile function and myosin structural distribution

Moran, Amy L., Warren, Gordon L., Lowe, Dawn A.·2006·Journal of Applied Physiology
DOI 10.1152/japplphysiol.01029.2005
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Abstract

The purposes of this study were to determine the effects of ovarian hormone removal on force-generating capacities and contractile proteins in soleus and extensor digitorum longus (EDL) muscles of mature female mice. Six-month-old female C57BL/6 mice were randomly assigned to either an ovariectomized (OVX; n = 13) or a sham-operated (sham; n = 13) group. In vitro contractile function of soleus and EDL muscles were determined 60 days postsurgery. Total protein and contractile protein contents were quantified, and electron paramagnetic resonance (EPR) spectroscopy was used to determine myosin structural distribution during contraction. OVX mice weighed 15% more than sham mice 60 days postsurgery, and soleus and EDL muscle masses were 19 and 15% greater in OVX mice, respectively ( P ≤ 0.032). Soleus and EDL muscles from OVX mice generated less maximal isometric force than did those from sham mice [soleus: 0.27 (SD 0.04) vs. 0.22 N·cm·mg−1 (SD 0.04); EDL: 0.33 (SD 0.04) vs. 0.27 N·cm·mg−1 (SD 0.04); P ≤ 0.006]. Total and contractile protein contents of soleus and EDL muscles were not different between OVX and sham mice ( P ≥ 0.242), indicating that the quantity of contractile machinery was not affected by removing ovarian hormones. EPR spectroscopy showed that the fraction of strong-binding myosin during contraction was 15% lower in EDL muscles from OVX mice compared with shams [0.277 (SD 0.039) vs. 0.325 (SD 0.020); P = 0.004]. These results indicate that the loss of ovarian hormones has detrimental effects on skeletal muscle force-generating capacities that can be explained by altered actin-myosin interactions.

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Crossref · 10.1152/japplphysiol.01029.2005

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  • Animal models of low estrogen levels, which occur after ovariectomy (OVX), reveal an increase in overall body mass and in the mass of individual muscles.

    “Animal models of low estrogen levels, which occur after ovariectomy (OVX), reveal an increase in overall body mass and in the mass of individual muscles (68).”

    From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [68]

  • OVX leads to an increase in overall body weight and muscle weight, and a 70% increase in ERα mRNA with no change in ERβ mRNA.

    “OVX leads to an increase in overall body weight and muscle weight (68), and a 70% increase in ERα mRNA with no change in ERβ mRNA (7).”

    From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [68]

  • The fraction of strong binding myosin in OVX muscle is 0.263 or ∼15% lower than baseline. Following estrogen treatment, the fraction of strongly bound myosin increases to 0.311, which is indistinguishable from sham levels (0.300).

    “The fraction of strong binding myosin in OVX muscle is 0.263 or ∼15% lower than baseline (67, 68). Following estrogen treatment, the fraction of strongly bound myosin increases to 0.311, which is indistinguishable from sham levels (0.300) (67).”

    From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [68]

  • Analysis of contractile function in OVX mice reveals a significant decrease in maximal isometric tetanic force from sham levels (180 mN) to 168 mN. This decline is attenuated to 187 mN with estrogen supplementation.

    “Analysis of contractile function in OVX mice reveals a significant decrease in maximal isometric tetanic force from sham levels (180 mN) to 168 mN (67, 68). This decline is attenuated to 187 mN with estrogen supplementation (67).”

    From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [68]

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Authors
Moran, A. L., Warren, G. L., & Lowe, D. A.
Affiliations
  • University of Minnesota (US)
  • Georgia State University (US)
ORCID
Venue
Journal of Applied Physiology
Publisher
American Physiological Society
Published
2006-02
Volume
100
Issue
2
Pages
548-559
ISSN
  • 0161-7567 (ISSN-L)
  • 8750-7587
  • 1522-1601
DOI
10.1152/japplphysiol.01029.2005
OpenAlex
W2071620311
Cited by
123
References
38
Citations by year
2026: 6 · 2025: 5 · 2024: 8 · 2023: 8 · 2022: 4 · 2021: 4 · 2020: 5 · 2019: 8 · 2018: 9 · 2017: 5 · 2016: 3 · 2015: 8 · 2014: 7 · 2013: 6 · 2012: 5
FWCI
0.75
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Applied Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institute on Aging (R03AG025861, K01AG020990, AG-20990)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (T32 AR-07612, T32AR007612)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
Keywords
ovariectomy, ovarian hormones, estrogen deficiency, skeletal muscle contractile function, soleus muscle, extensor digitorum longus, actin-myosin interaction, isometric force, muscle mass, contractile proteins, female mice
MeSH terms
Actins, Animals, Female, Hindlimb, Hypertrophy, Mice, Inbred C57BL, Actin Cytoskeleton, Muscle Contraction, Ovariectomy, Muscle, Skeletal, Skeletal Muscle Myosins, Gonadal Hormones, Mice

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