Estrogen receptor-α and -β and aromatase knockout effects on lower limb muscle mass and contractile function in female mice
Open accessAbstract
Estrogen (E 2) is reported to regulate skeletal muscle mass and contractile function; whether E 2 exerts its effects through estrogen receptor-α (ERα) or -β (ERβ) is unclear. We determined the effect of ERα or ERβ elimination on muscle mass and contractile function in multiple muscles of the lower limb, muscles with different locomotor tasks and proportions of fiber types I and II: soleus (Sol), plantaris (Plan), tibialis anterior (TA), and gastrocnemius (Gast) in mature female mice. To determine E 2 elimination effects on muscle, we also used aromatase (Ar) knockout (KO) and wild-type (WT) mice. ERα and ArKO body weights were ∼10 and 20% higher than WT. Although muscle mass tended to show a commensurate increase in both groups, only the TA was significantly larger in ERα ( P < 0.05). Ratios of muscle mass to body mass revealed significantly lower values for Gast and TA in ArKO mice ( P < 0.05). Tetanic tension (P o) per calculated anatomical cross-sectional area (aCSA) in ERα KO was lower in TA and Gast than in WT. Lower P o/aCSA in ERα KO Gast and TA was also supported histologically by significantly less P o/fiber areas ( P < 0.05). ArKO mice also had lower P o/aCSA in Gast and TA compared with WT. ERβ KO and WT mice were comparable in all measures. Our results support the hypothesis that E 2 effects on skeletal muscle are mediated in part via the ERα but that E 2 effects may be mediated via more than one mechanism or receptor.
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Skeletal muscle expresses two forms of ERs (α and β), and the effects of estrogen are mediated, in part, through these receptors.
“Skeletal muscle expresses two forms of ERs (α and β), and the effects of estrogen are mediated, in part, through these receptors (14, 21, 56, 98).”
From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [14]
ERα-null mice have decreased tetanic tension of gastrocnemius and TA muscles in females compared with wild-type controls.
“ERα-null mice have decreased tetanic tension of gastrocnemius and TA muscles in females compared with wild-type controls (14).”
From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [14]
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- Authors
- Brown, M., Ning, J., Ferreira, J. A., Bogener, J. L., & Lubahn, D. B.
- Affiliations
- University of Missouri (US)
- ORCID
- Ning, Jie
- Venue
- American Journal of Physiology-Endocrinology and Metabolism
- Publisher
- American Physiological Society
- Published
- 2009-04
- Volume
- 296
- Issue
- 4
- Pages
- E854-E861
- ISSN
- 0193-1849 (ISSN-L)
- 1522-1555
- PMC
- PMC2670624
- OpenAlex
- W2169782763
- Cited by
- 60
- References
- 52
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 2 · 2021: 4 · 2020: 3 · 2019: 4 · 2018: 5 · 2017: 3 · 2016: 7 · 2015: 7 · 2014: 1 · 2013: 6 · 2012: 3
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- American Journal of Physiology-Endocrinology and Metabolism (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Environmental Health Sciences (P01ES010535, P01 ES 10535)
- National Center for Complementary and Integrative Health (R01AT002978)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R21HD058834, HD058834)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Keywords
- estrogen receptor alpha, estrogen receptor beta, estrogen, skeletal muscle mass, muscle contractility, lower limb muscles, tibialis anterior, gastrocnemius, soleus muscle, plantaris, tetanic tension, muscle fiber composition, female mice
- MeSH terms
- Animals, Aromatase, Body Weight, Estradiol, Female, Mice, Inbred C57BL, Muscle Contraction, Muscle Proteins, Organ Size, Mice, Knockout, Muscle, Skeletal, Muscle Fibers, Skeletal, Lower Extremity, Estrogen Receptor alpha, Estrogen Receptor beta, Mice
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