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Cited by 103Estrogen and related hormone effectsHormonal and reproductive studiesAdrenal Hormones and Disorders

Effects of estrogen and progesterone administration on extracellular fluid.

Stachenfeld, Nina S., Taylor, Hugh S.·2004·Journal of Applied Physiology
DOI 10.1152/japplphysiol.01032.2003
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Abstract

To determine the effect of estrogen and progesterone on plasma volume (PV) and extracellur fluid volume (ECFV), we suppressed endogenous estrogen and progesterone by using the gonadotropin-releasing hormone (GnRH) antagonist ganirelix acetate in seven healthy women (22 ± 1 yr). Subjects were administered GnRH antagonist for 16 days. Beginning on day 5 of GnRH antagonist administration, subjects were administered estrogen (E 2) for 11 days, and beginning on day 12 of GnRH antagonist administration, subjects added progesterone (E 2-P 4) for 4 days. On days 2, 9, and 16 of GnRH antagonist administration, we estimated ECFV (inulin washout), transcapillary escape rate of albumin (TER alb), and PV (Evans blue dye). Plasma E 2 concentration increased from 17.9 ± 4.5 (GnRH antagonist) to 195.9 ± 60.1 (E 2, P < 0.05) to 245.6 ± 62.9 pg/ml (E 2-P 4, P < 0.05). Compared with GnRH antagonist (1.3 ± 0.5 ng/ml), plasma P 4 concentration was unchanged during E 2(0.9 ± 0.3 ng/ml) and increased to 9.4 ± 3.1 ng/ml during E 2-P 4( P < 0.05). Both E 2(44.1 ± 3.1 ml/kg) and E 2-P 4(47.7 ± 2.8 ml/kg) increased PV compared with GnRH antagonist (42.8 ± 1.3 ml/kg, P < 0.05). Within-subjects TER alb was a strong negative predictor of PV (mean r = 0.92 ± 0.03, P < 0.05), and TER alb was lowest during E 2-P 4(5.7 ± 0.5, 4.1.0 ± 1.1, and 2.8 ± 0.9%/h, P < 0.05, for GnRH antagonist, E 2, and E 2-P 4, respectively). ECFV was reduced during E 2(227 ± 31 ml/kg, P < 0.05) compared with both GnRH antagonist (291 ± 37 ml/kg) and E 2-P 4(283 ± 19 ml/kg). Thus the percentage of extracellular fluid in the plasma compartment increased to 21.0% ( P < 0.05) during E 2 compared with GnRH antagonist (16.1%) and E 2-P 4(17.2%) admistration. Thus E 2 increased PV via actions on the capillary endothelium to lower TER alb and favor intravascular water retention, whereas during E 2-P 4 PV increased via the combined responses of ECFV expansion and lower TER alb.

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Authors
Stachenfeld, N. S., & Taylor, H. S.
Affiliations
Yale University (US)
ORCID
Venue
Journal of Applied Physiology
Publisher
American Physiological Society
Published
2004-03
Volume
96
Issue
3
Pages
1011-1018
ISSN
  • 0161-7567 (ISSN-L)
  • 8750-7587
  • 1522-1601
DOI
10.1152/japplphysiol.01032.2003
OpenAlex
W2143893112
Semantic Scholar
6fb33232e376ee898792a20cb8913c3c80272a2a
Cited by
103
References
42
Citations by year
2026: 5 · 2025: 4 · 2024: 6 · 2023: 11 · 2022: 5 · 2021: 5 · 2020: 3 · 2019: 6 · 2018: 5 · 2017: 2 · 2016: 5 · 2015: 3 · 2014: 3 · 2013: 2 · 2012: 5
FWCI
2.13
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Applied Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Heart, Lung, and Blood Institute (R01 HL-071159-01A1, R01-HL-62240-01A1, R01HL062240, R01HL071159)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
Keywords
estrogen, progesterone, extracellular volume, plasma volume, Evans blue, ganirelix, GnRH antagonist, women, fluid balance, plasma protein binding, albumin binding, estrogen-progesterone interaction, hormone replacement therapy, hyperlipidemia
MeSH terms
Adult, Estrogens, Female, Humans, Gonadotropin-Releasing Hormone, Luteal Phase, Plasma Volume, Progesterone, Extracellular Fluid

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