Acid Stress Triggers Resistance to Acetic Acid-Induced Regulated Cell Death through Hog1 Activation Which Requires RTG2 in Yeast
Open accessAbstract
Acid stress causes resistance to acetic acid-induced regulated cell death (AA-RCD) in budding yeast, resulting in catalase activation. In order to explore the molecular determinants of evasion of AA-RCD triggered by acid stress adaptation, we studied the involvement and the possible interplay of the master regulator of transcription high-osmolarity glycerol 1 (HOG1) andRTG2, a positive regulator of theRTG-dependent mitochondrial retrograde signaling. Viability, DNA fragmentation, and ROS accumulation have been analyzed in wild-type and mutant cells lackingHOG1and/orRTG2. Catalase activity and transcription ofCTT1andCTA1, coding the cytosolic and peroxisomal/mitochondrial catalase, respectively, as well asHog1phosphorylation, were also analyzed. Our results show thatHOG1is essential for resistance to AA-RCD and its activation results in the upregulation ofCTT1, but notCTA1, transcription during acid stress adaptation.RTG2is required forHog1-dependentCTT1upregulation upon acid stress, despite failure ofRTGpathway activation. We give evidence thatRtg2has a cytoprotective role and can act as a general cell stress sensor independent of Rtg1/3-dependent transcription.
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An earlier study proposed a hormetic induction of antioxidant defense mediated by the Rtg pathway in S. cerevisiae, which was subsequently shown to involve cooperation between the HOG and Rtg pathways.
“An earlier study (Torelli et al. 2015) proposed a hormetic induction of antioxidant defense mediated by the Rtg pathway in S. cerevisiae, that was subsequently shown to involve the cooperation between the HOG and Rtg pathways (Guaragnella et al. 2019).”
From Mitochondrial retrograde control of transcription evolves with respiratory stress, metabolic adaptation, and virulence in budding yeasts (2026) · cites this paper as Guaragnella et al. 2019
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- Authors
- Guaragnella, N., Stirpe, M., Marzulli, D., Mazzoni, C., & Giannattasio, S.
- Affiliations
- Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies (IT)
- Istituto Pasteur (IT)
- Corresponding author
- Guaragnella, Nicoletta
- Giannattasio, Sergio
- Venue
- Oxidative Medicine and Cellular Longevity
- Publisher
- Wiley
- Published
- 2019-02-25
- Volume
- 2019
- Pages
- 1-9
- ISSN
- 1942-0994 (ISSN-L)
- 1942-0900
- PMC
- PMC6410445
- OpenAlex
- W2917093278
- Cited by
- 37
- References
- 38
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 10 · 2023: 6 · 2022: 4 · 2021: 8 · 2020: 1
- FWCI
- 1.08
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Oxidative Medicine and Cellular Longevity (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- IRIS Research product catalog (Sapienza University of Rome) (submittedVersion, cc-by)
- CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion, cc-by-nc-nd)
- Funders
- Progetto POR Puglia FESR-FSE (1JLZKD0)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- acid stress, Hog1, catalase, CTT1, DNA fragmentation, reactive oxygen species, Saccharomyces cerevisiae, stress adaptation, cell viability
- MeSH terms
- Saccharomyces cerevisiae, Signal Transduction, Cell Death, Acetic Acid, Mitogen-Activated Protein Kinases, Saccharomyces cerevisiae Proteins, Intracellular Signaling Peptides and Proteins
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